• Nutrition Research and Practice
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• 제7권3호
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• pp.185-191
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• 2013

In previous studies, we found that the consumption of legumes decreased bone turnover in ovariectomized rats. The purpose of the present study is to determine whether the protective effects on bone mineral density (BMD) and the microarchitecture of a diet containing legumes are comparable. In addition, we aim to determine their protective actions in bones by studying bone specific gene expression. Forty-two Sprague-Dawley rats are being divided into six groups during the 12 week study: 1) rats that underwent sham operations (Sham), 2) ovariectomized rats fed an AIN-93M diet (OVX), 3) ovariectomized rats fed an AIN-93M diet with soybeans (OVX-S), 4) ovariectomized rats fed an AIN-93M diet with mung beans (OVX-M), 5) ovariectomized rats fed an AIN-93M diet with cowpeas (OVX-C), and 6) ovariectomized rats fed an AIN-93M diet with azuki beans (OVX-A). Consumption of legumes significantly increased BMD of the spine and femur and bone volume of the femur compared to the OVX. Serum calcium and phosphate ratio, osteocalcin, expression of osteoprotegerin (OPG), and the receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL) ratio increased significantly, while urinary excretion of calcium and deoxypyridinoline and expression of TNF-${\alpha}$ and IL-6 were significantly reduced in OVX rats fed legumes, compared to OVX rats that were not fed legumes. This study demonstrates that consumption of legumes has a beneficial effect on bone through modulation of OPG and RANKL expression in ovariectomized rats and that legume consumption can help compensate for an estrogen-deficiency by preventing bone loss induced by ovarian hormone deficiency.

• 생약학회지
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• 제37권4호
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• pp.324-330
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• 2006

The aim of this study was to evaluate the effects of Artemisia Princeps Var. Orientalis (AP) on serum lipids and the collagen content of the connective tissues in ovariectomized rats. From day 2 until day 37 after the ovariectomy, Sprague-Dawley female rats were randomly assigned to the following groups: sham-operated rats (Sham), ovariectomized control rats (OVX-control), and ovariectomized rats supplemented with the AP 50 mg/kg bw/day (OVX-AP). The AP ethanol extracts were orally administrated 1 mL per day. The OVX rats were significantly heavier than the sham-operated rats at all time points, but supplementation with the AP extracts tended to gain weight less than OVX-control. Although total-cholesterol was increased at OVX-control, supplementation with the AP extracts tended to result in less than OVX-control. Triglyceride was significantly decreased after supplemented with the AP extracts (p<0.05). HDL-cholesterol is appeared higher AP extracts group than OVX-control. According to the results, we could know the fact that AP extracts were effective on serum lipids content throughout decreasing total-cholesterol, triglyceride and increasing HDL-cholesterol in ovariectomized rats. Supplementation with the AP extracts prevented a decrease in the collagen level in bone and cartilage tissues. These results are consistent with the conclusions based on the estrogenic activities of AP. Therefore, it may be used to possibly improve the quality of life in menopausal women.

• 생명과학회지
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• 제9권2호
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• pp.216-221
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• 1999

In order to observe the bioactivity of ovariectomized rats, ovariectomized group (Ovx), nonovariectomized group(Sham) and ovariectomized aloe-treat group(Ovx＋Aloe) were made. Lipidperoxides of liver and kidney, serum AST.ALT, BUN, total cholesterol and HDL-cholesterol were investigated as follows. Lipidoxides of the liver and the kidney in Ovx group were 1.74 times and 1.38 times increased compared to Sham group respectively. But they were significantly decreased in Ovx+Aloe group compared to Ovx group. Serum AST and ALT in Ovx group were increased 1.49 times and 1.65 times respectively compared to Sham group respectively. But they were decreased compared to Ovx group. Serum total cholesterol in Ovx group was increased 1.48 times compared to Sham group. While it was increased 50% in Ovx＋Aloe group. Serum HDL-cholesterol in Ovx group was decreasd 23$\%$ compared to Sham group. While it was increasd 58% in Ovx＋Aloe group compared to Ovx group. Serum BUN in Ovx group was increased slightly compared to Sham group. In Ovx＋Aloe serum BUN had no change compared to Ovx group.

• 운동영양학회지
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• 제13권1호
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• pp.37-44
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• 2009

The purpose of this study was to investigate the change of bone metabolic parameters between climbing exercise training and treadmill running training with alpha lipoic acid supplementation in ovariectomized rats. Fifty-six Sparague-Dawley, 8 weeks of age, were assigned into nine groups: normal control at pre-test (Pre-NC), normal control at post-test (NC), sham-sedentary (SS), ovariectomized-control (OVX-Con), ovariectomized-ALA inject (OVX-ALA), ovariectomized-climbing exercise (OVX-CE), ovariectomized-ALA inject+climbing exercise (OVX-ACE), ovariectomized-treadmill exercise (OVX-TE) and ovariectomized-ALA inject+treadmill exercise (OVX-ATE). The climbing exercise group voluntarily climbed the 200 cm tower to drinking water from the bottle set at the top of it. The treadmill exercise group was performed the 60 min treadmill running with grade 6-8%, and velocity 10-15 m/min. The frequency of exercise training was performed 6 days per wk in both training intervention. For the comparison of bone metabolic responses following to different training intervention, the strength, length and weight of femur, total bone density, bone mineral contents, deoxypyridioline, osteocalcin and parathyroid hormone, estradiol and lutenizing hormone were measured in this study. The body weight was higher in the OVX-Con, OVX-ALA and OVX-TE groups as compared to NC group. The total bone mineral density of OVX-ACE showed a higher value than SS, OVX-Con, OVX-ATE and OVX-TE. But urine and blood metabolic parameters showed no significance among groups. In conclusion, this study results suggested that climbing exercise training and alpha lipoic acid supplementation may an effective intervention for prevention of osteoporosis in ovriectomized rats.

• Asian-Australasian Journal of Animal Sciences
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• 제20권8호
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• pp.1266-1271
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• 2007

This study examined effects of calcium supplemented milk on bone loss in ovariectomized rats. Twenty four Sprague-Dawley female rats, 7 weeks-old, were divided into 4 groups, ovariectomized and fed diets containing: 1) control, no Ca supplemented milk, 2) ovx 1, Ca carbonate supplemented milk, 3) ovx 2, ionized Ca supplemented milk, and 4) ovx 3, nano Ca supplemented milk. All rats were fed 1 ml of milk containing 20 mg supplemented Ca. After 18 wk feeding, body weight gain and food efficiency ratio were significantly different between ovx 1 and ovx 3. Serum concentration of calcium and phosphorus were not different among groups. However, there was a significant difference in calcium content of dry femoral weight in ovx 3 compared with the control and ovx 2. In addition, femoral bone mineral density ($g/cm^2$) was significantly greater in ovx 3 than in other groups (p<0.05). The ovx 3 group showed the highest stiffness (N/mm), maximum energy (N) in femur and trabecular bone area (%). The present study indicated that nano Ca supplementation in milk may be an effective way to enhance bone calcium metabolism for ovariectomized rats.

• 생명과학회지
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• 제18권1호
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• pp.46-51
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• 2008

석류(Punica granatum L.)는 생리활성이 강한 천연성분인 폴리페놀과 탄닌이 많이 함유되어 있어 예로부터 전통요법의 치료제로 사용되어 왔으며, 최근에는 석류의 phytoestrogen을 이용하여 갱년기 치료에 대한 연구가 활발히 진행중이다. 따라서 본 연구에서는 석류의 씨와 껍질의 ethanol 추출물과 석류 껍질 분획물을 이용하여 인위적으로 난소를 절제하여 폐경을 야기 시킨 흰쥐에서의 혈 중 지질 농도의 변화를 검토하였다. 그 결과 혈 중 total-cholesterol 및 triglyceride 농도에 있어서는 석류 ethanol추출물 투여에 의한 유의적인 효과는 없었으나, triglyceride의 경우 석류 껍질 추출물의 $200mg/kg{\cdot}bw/day$투여군에서는 OVX-control에 비하여 낮게 나타났다. 또한 혈 중 HDL-cholesterol농도는 석류씨와 껍질부분을 추출한 모든 실험군에서 동맥경화의 지표로서 관상성 심장질환에 대한 방어 작용을 가지고 있는 HDL-cholesterol 함량이 높게 나타났다. 특히 석류 껍질부분의 ethanol추출물의 경우 OVX-control에 비하여 유의적으로 나타나 갱년기장애로 인한 심혈관계 질환을 예방하는 천연 에스트로겐 대체 식품으로서의 귀추가 주목된다.

• 생명과학회지
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• 제18권2호
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• pp.249-254
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• 2008

폐경 후 여성의 비만은 독특한 생리적 현상으로 음식물 섭취가 촉진되고, 몸무게와 지방조직이 증가되고, 정상 여성보다 혈류 관련 질환 발병이 높아지는 것으로 알려져 있다. 이에 본 연구에서는 갱년기를 유도한 흰쥐에 다시마 추출물을 투여하여 체내 지질 함량 변화에 대해 그 효과를 검토하였다. 그 결과 난소를 절제한 OVX-control군이 난소를 절제하지 않은 Sham군보다 체중이 뚜렷이 증가하는 경향을 보였으며, 다시마 추출물이 체중감소에는 크게 영향을 미치지 않았다. GOT 활성에서는 난소를 절제한 OVX-control군이 난소를 절제하지 않은 Sham군에 비해 GOT 활성이 증가하였으나, 난소를 절제한 후 다시마 추출물 투여에 의해 난소를 절제하지 않은 Sham군보다 GOT활성이 더 감소하는 결과가 나타났다. GPT 활성에서는 난소를 절제 한 후 다시마 추출물을 투여한 군에서는 OVX-control군에 비해 감소하는 경향을 보였는데, 특히 OVX-ST200군에서 감소하는 결과가 나타났다. 난소 절제 후 다시마 추출물 투여는 난소 절제로 인해 야기되는 혈중 총 콜레스테롤의 함량 및 혈청 지질 농도의 증가를 감소시켰다. 또한, HDL-콜레스테롤은 난소절제 군에 비해 난소절제 후 다시마 추출물을 투여하였을 때 혈중 함량이 증가하였다. 이상의 결과에서 다시마 추출물이 갱년기 장애 시 발생할 수 있는 혈 중 지질 함량 변화에 효과가 있는 것으로 나타나 혈류 관련 질환에 대한 개선 식품으로의 활용이 기대된다.

• 생명과학회지
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• 제15권5호
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• pp.834-839
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• 2005

여성은 폐경 시 음식물 섭취가 증가하여 체중이 증가되고, 정상 여성보다 혈류관련질환 발병이 높아진다. 이에 본 연구에서는 갱년기 장애시 유발되는 체내 지질 함량 증가에 있어 오디 추출물이 미치는 영향을 조사하기 위해 인위적 폐경을 유발시킨 난소절제 횐쥐에 오디추출물을 투여하여 그에 따른 혈 중 효소 활성 및 지질 함량에 미치는 영향을 검토하였다. 그 결과 난소 절제 후 오디추출물 투여는 난소절제로 인해 야기되는 혈 중 total- cholesterol 함량 및 혈청 지질농도의 증가를 감소하였다. 하지만 HDL-cholesterol은 난소절제 군에 비해 난소절제 후 오디추출물을 투여할 경우 그 수치가 증가하였으며, LDL-cholesterol은 반대로 감소하는 것을 볼 수 있어 오디추출물이 갱년기 장애 시 발생할 수 있는 혈 중 지질함량 변화에 효과가 있는 것으로 나타나 혈류관련질환에 대한 개선 식품으로의 활용이 기대된다.

• 한국동물위생학회지
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• 제33권3호
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• pp.309-312
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• 2010

This was conducted to determine the effects of body weight, organ weight, hematological values and biochemical parameters by L-carnitine in the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group (n=10) received operation and no treatment. OVX+Carn group (n=10) received operation and L-carnitine. Body weight was significantly lower in OVX+Carn group than in all other groups. Also, organ weight such as heart, liver, spleen and kidney was measured. The heart and spleen weight were significantly lower in the OVX+Carn group than in the Intact and Sham group. The liver weight in the OVX+Carn group was significantly differences in comparison with those in the other groups. Also, there was significantly differences in the organ weight of kidney between in the OVX+Carn group and in the other groups. The hematological values of WBC, RBC, MCV, MCH and MCHC were no significant differences in any other groups. The total cholesterol, triglyceride and high density lipoprotein decreased significantly in the OVX+Carn group as compared to those in the OVX group. But, there were no significant differences in low density lipoprotein in any other groups. We conclude that L-carnitine enhanced the body weight in the ovariectomized rats. Our findings suggest that L-carnitine may influence the process of absorption of fat in the ovariectomized rats.

• 생명과학회지
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• 제15권6호
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• pp.1028-1033
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• 2005

여성의 폐경에 의한 eskogen의 감소는 심혈관계질환을 빠르게 진행시킨다고 알려져 있다. 최근 새로운 식품으로 각광받고 있는 매생이 추출물을 갱년기장애를 일으킨 횐쥐에 투여하여 혈 중 지질 수준변화와 항 혈소판 응집 능을 통해서 그 효과를 검토해 보았다. 매생이 추출물을 갱년기를 유도한 횐쥐에 투여하여 혈소판 응집 능에 미치는 영향을 검토한 결과 난소절제에 의하여 혈소판응집이 활성화되었는데, 매생이 추출물을 투여한 군에서 혈소판응집이 억제되는 것을 확인할 수 있었다. 또한 난소 절제에 의해 total-cholesterol의 함량이 증가되었는데, 이는 매생이 추출물을 투여함으로써 감소하는 경향을 보였다. 혈 중 중성 지방에서는 매생이을 투여한 모든 군에서 난소를 절제하지 않는 군과 비슷한 함량으로 감소하였고, 또한 혈중 HDL-cholesterol은 매생이 추출물 모두 난소 절제한 군과 비교해서 높은 경향을 나타내어 매생이 추출물이 혈액과 관련된 심혈관계 질환에 유효한 효과를 나타낼 것으로 사료된다.