• Journal of Nutrition and Health
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• 제41권4호
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• pp.299-306
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• 2008

The purpose of this research was to investigate the effect of the calcium intake on lipid profile and antioxidant capacities in ovariectomized (OVX) rats. Rats were divided into 3 groups and fed diet with different levels of calcium (low 0.1%, adequate 0.5%, high 1.5%) for 4 weeks. The half of rats in each group was ovariectomized and the others were sham-operated. And rats were fed same diets for 8 weeks after operation. Feed intake and weight gain were significantly higher in OVX group than those in sham-operated. Serum HDL-cholesterol was the highest in high-calcium group of OVX. Hepatic triglyceride of low-calcium group in sham-operated was the highest, while that of highcalcium group in OVX was the highest. Hepatic activities of glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), and catalase were significantly decreased by increasement of calcium intake. Hepatic TBARS level was the lowest in high-calcium group of OVX. And hepatic level of TBARS induced by AAPH was significantly decreased by increasement of calcium intake. These results may indicate that the high calcium intake have the potential role to improve lipid profiles and antioxidant capacities in OVX rats.

• 한국동물위생학회지
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• 제33권4호
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• pp.411-415
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• 2010

The present study was devised to determine the effects of body weight, organ weight, hematological values and biochemical parameters by vitamin E and selenium (Selevit) in the ovariectomized (OVX) rats. The animals were divided into 4 groups. Intact group (n=10) received no treatment and operation. Sham group (n=10) received only sham operation and no treatment. OVX group received operation and no treatment. OVX+Selevit received operation and Selevit. The body weights of the all group increased, and that of OVX+Selevit group was the lowest rates. There were significant differences (P<0.01, P<0.001) of body weights between OVX+Selevit group and all other groups. Also, organ weights such as heart, liver, spleen and kidney were measured. The heart and liver weight were significantly lower (P<0.01, P<0.001) in the OVX+Selevit group than in the Intact and Sham group. Also, the kidney weight was significantly lower (P<0.05, P<0.01) in the OVX+Selevit group than in the all other group. On the other hand, there was no significantly difference in the organ weight of spleen between the OVX+Selevit group and the other groups. The number of WBC was significantly higher (P<0.05) in the OVX+Selevit group than in the all other groups. The hematological values of RBC, MCV, MCH and MCHC were no significant differences in any groups. The biochemical parameters of serum total protein and alkaline phosphatase increased significantly in the OVX+Selevit group as compared to that in the OVX group. But, there were no significant differences in AST and ALT in any groups. We conclude that vitamin E and selenium were significantly decreased the body weights in the ovariectomized rats. Our findings suggest that vitamin E and selenium may influence the process of lipid packaging and absorption in the ovariectomized rats.

• 생명과학회지
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• 제18권11호
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• pp.1578-1583
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• 2008

폐경을 앞둔 여성에게는 폐경을 전후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적 폐경을 유발시킨 난소 절제 쥐에서의 골 손실의 유무를 결합조직 중의 collagen 함량 변화를 통하여 그 효과를 검토하였다. 그 결과 생화학적 골 대사 지표인 혈 중 ALP 활성은 난소 절제(OVX-control)로 인하여 증가하는 반면, 난소 절제 후 다시마 추출물 투여(OVX-ST50, OVX-ST200)로 혈중 ALP 활성은 감소하는 경향을 보여 골 흡수를 저하시킬 수 있는 가능성을 나타내었다. 한편 난소 절제 후 collagen 함량이 감소하였으나, 다시마 추출물 투여에 의해 피부조직과 골.연골 조직에서 collagen 함량이 증가하는 경향을 보였고, 특히 OVX-ST200군은 각 조직 모두 OVX-control에 비하여 유의적으로 증가하였다(p<0.05). Collagen의 성숙 가교물질인 pyridinoline 함량 또한 난소 절제 후 골, 연골 조직 중에서 감소하였으나 다시마 추출물을 투여한 군에서 증가하는 경향을 나타내었다. 골에서의 deoxypylidinoline 함량은 난소를 절제하지 않은 Sham군이 OVX-control군에 비해서 유의적으로 높은 값을 나타냈고(p<0.05), 난소 절제 후 다시마 추출물을 농도별로 투여한 군(OVX-ST50 및 OVX-ST200)에서 유의 적으로 높은 값을 나타내어(p<0.05), 난소 절제 시 합성이 감소되는 deoxypyridinoline 생성량을증가시켰다. 에스트로겐 감소로 인한 결합조직 중 collagen 함량 저하가 다시마 추출물 투여에 의해 증가된 본 실험 결과로 미루어 보아 다시마 중의 phytoestrogen이 에스트로겐 유사효과를 나타냄을 알 수 있었다. 이상과 같이 폐경으로 인한 장애를 감소시키기 위하여 에스트로겐의 장기간 복용에 의한 부작용을 다시마 중의 phytoestrogen이 estrogen 대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요 한 것으로 생각된다.

• Food Science and Biotechnology
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• 제14권2호
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• pp.242-248
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• 2005

The effect of calcium and boron supplementation on bone strength was determined in growing and ovariectomized (OVX) Sparague-Dawley rats. Rats were divided into 9 groups and fed diet with different intake levels of calcium and boron for 4 weeks. About fifty percentages of rats in each group were OVX and the others were sham-operated. The rats were fed same diets after operation for 8 weeks. The feed intake, body weight gain, and FER were significantly higher in OVX rats than those in sham-operated ones. Serum osteocalcin, bone formation biomarker, was significantly increased with increment in calcium and boron intakes. Serum estradiol was lower in OVX rats than in sham-operated ones. Bone mineral density of femur was significantly lower in OVX rats than in other group. The breaking forces of bones were not significantly different among the groups. The urinary excretion of deoxypyridinoline, osteolytic marker was significantly increased with increment in calcium intake and ovariectomy. The urinary calcium excretion was significantly increased with increment in calcium intake, but decreased with increment in boron intake. According to theses results, the boron supplementation resulted in higher serum osteocalcin and lower urinary calcium excretion. Therefore, it could be suggested that the boron supplementation may be complementary and useful to calcium nutrition for bone health.

• 생명과학회지
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• 제14권2호
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• pp.356-361
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• 2004

폐경과 더불어 시작되는 폐경기의 에스트로겐 변화로 발생되는 건강 문제 중 심혈관계질환이 문제점으로 대두되고 있다. 그러므로 본 연구는 갱년기 장애 시 유발되는 체내 지질 함량 증가에 있어서 김 추출물이 미치는 영향을 조사하기 위해 인위적 폐경을 유발시킬 수 있는 난소 절제 쥐에서 김 추출물의 혈 중 지질 함량 변화에 미치는 영향을 검토하였다. 그 결과 난소 절제에 의한 total-cholesterol의 증가가 김 추출물을 투여함으로써 감소하는 경향을 보였다. 특히 김 추출물 50 mg/kg bw/day에서 유의적으로 감소하였다. 혈 중성 지방에서는 김 추출물 농도별 투여한 OVX-PT50, OVX-PT200 모든 군에서 난소를 절제하지 않은 Sham군과 비슷한 수준으로 감소하는 경향을 보였고, 또한 혈 중 HDL-cholesterol은 김 추출물 투여로 인해 난소 절제한 군과 비교해서 높은 경향을 나타내었다. 이상의 결과는 갱년기 장애 시 유발되는 지질 대사 이상으로 오는 심혈관계질환에 김의 섭취가 유익한 결과를 줄 것으로 사료되며, 또한 잘못된 식습관으로 오는 비만이나 심혈관계 질환에도 유익한 효과를 가져다 줄 것으로 기대된다.

• 한국약용작물학회지
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• 제20권1호
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• pp.42-46
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• 2012

Soy isoflavones have been suggested to improve bone loss and lipid profile in postmenopausal women and ovariectomized rats. In present study, we investigated the hypothesis that consumption of soybean, mung bean, cowpea and azuki bean has a beneficial effect on lipid profile which associates with bone mass in ovariectomized rats. Forty two female Sprague-Dawley rats were either sham-operated (Sham) or surgically ovariectomized (OVX). Sham and OVX groups were fed a regular AIN-93M diet, but ovariectomized rats with soybean (OS), mung bean (OM), cowpea (OC) or adzuki bean (OA) were fed AIN-93M diet replacing 35% of corn starch with powdered OS, OM, OC or OA for 10 weeks. Total- and LDL-cholesterol concentrations were significantly lower in Sham and OC groups than other OVX groups. There was significant negative association between total cholesterol concentration and bone mineral density (BMD) of tibia in only OC group. In conclusion, total-cholesterol concentration was significantly and negatively correlated with BMD in rats consumed cowpea, suggesting that the reduced cholesterol concentration may have a beneficial effect on bone mass.

• Nutrition Research and Practice
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• 제2권1호
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• pp.13-16
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• 2008

Taurine supplementation has been shown to have an effect on lowering blood lipids in ovariectomized (OVX) rats. It therefore seemed desirable to find out whether the beneficial effect of taurine on OVX rats fed calcium-deficient diet could also be reproduced. Forty female Sprague-Dawley rats were divided into two groups. One group was OVX and the other group received a sham operation (Sham). Each rat group was further divided into the control diet and the taurine supplemented (2.0g/100g diet) diet group. All rats were fed on calcium-deficient diet and deionized water ad libitum for 6 weeks. Plasma and liver lipids were determined by using commercial kits. LDL-cholesterol concentrations were estimated with the equation of Friedewald et al. (1972). There were no significant differences in body weight gain and food intake between the control and taurine group within Sham and OVX groups, but body weight gain, food intake, and food efficiency ratio was higher in the OVX group. Concentrations of plasma total cholesterol, triglyceride, LDL-cholesterol were significantly lower in the taurine fed group of OVX rats fed Ca deficient diet, while HDL-cholesterol concentration was increased in the taurine fed group. Therefore, in this study, we examined whether taurine also prevented hypercholesterolemia induced by ovarian hormone deficiency in ovariectomized rats when they were fed a calcium-deficient diet. These results indicate that taurine may have some beneficial effects on hypercholesterolemia and hypertriglyceridemia in OVX rats fed calcium-deficient diet.

• Food Science and Biotechnology
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• 제17권3호
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• pp.553-557
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• 2008

The effects of cheonggukjang and doenjang on bone mineral density, trabecular area and cortical thickness of the tibia, and serum osteocalcin level in ovariectomized rats were investigated. After 4 weeks, bone mineral density, bone trabecular area, the cortical thickness index, and serum osteocalcin level were analyzed. The cheonggukjang and doenjang diet groups showed significant prevention of ovariectomized (OVX)-related body weight gain. Whole body bone mineral density of the OVX group was significantly lower than that of the sham group, whereas the cheonggulgang and doenjang diets resulted in complete restoration of bone mineral density. Trabecular area in the proximal diaphysis and cortical thickness in the distal diaphysis of the tibia were increased significantly in the cheonggukjang and doenjang diet fed groups. The cheonggukjang and doenjang diets significantly reduced serum osteocalcin level in the OVX rats. These results suggest that cheonggukjang and doenjang might have inhibitory effects on osteoporosis, by showing accelerated bone formation in OVX rats.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1114-1119
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• 2003

폐경을 앞둔 여성에게는 폐경을 전후하여 지속되는 골 손실 가속화 등의 문제점을 가지고 있다. 그러므로 본 연구에서는 인위적 폐경을 유발시킬 수 있는 난소 절제 쥐에서의 골 손실의 유무를 결합조직 중의 collagen함량 변화를 통하여 그 효과를 검토하였다. 난소 절제 후 결합조직 중의 Collagen 함량은 난소를 절제하지 않은 Sham군에 비해 낮았으나 돌나물 추출물 투여에 의해 투여하지 않은 군보다 전체적으로 높게 나타났다. 난소 절제시 나타나는 결합조직 중 collagen 함량 저하에 대하여 돌나물 추출물의 작용 가능성이 밝혀졌으므로, 돌나물을 분획하여 그 효능을 검증하였다. 그 결과 생화학적 골 대사 지표인 혈 중 ALP 활성은 난소 절제로 (OVX-control)로 인하여 증가하는 반면, 난소 절제 후 돌나물 분획 투여 (OVX-EE, OVX-EA)로 혈 중 ALP 활성은 감소하는 경향을 보여 골 흡수를 저하시킬 수 있는 가능성을 나타내었다. 한편 난소 절제 후 돌나물 분획물 투여에 의해 폐 조직과 골$.$연골 조직에서 collagen 함량이 증가하는 경향을 보였고, 그 중 연골 조직에서는 collagen 함량의 저하가 정상적으로 회복되었다. 한편 피부 조직에서도 난소 절제 후 돌나물 분획물 투여군 모두에서 collagen 합성량이 유의적으로 증가하였다. 돌나물 추출물과 분획물이 에스트로겐 감소로 인한 Collagen 합성저하를 회복시킨 본 실험 결과로 미루어 보아 돌나물 중의 phytoestrogen이 에스트로겐 유사효과를 나타냄을 알 수 있었고, 외인성 에스트로겐 투여로 인한 부작용을 줄여 줄 수 있을 것으로 기대된다. 이상과 같이 폐경기 여성에게 돌나물의 섭취는 폐경으로 인한 장애를 감소시키기 위하여 에스트로겐의 장기간 복용에 의한 부작용을 돌나물 중의 phytoestrogen이 estrogen 대체 작용을 함으로써 그 부작용을 감소시켜 줄 것으로 사료되며, 이들의 구조 및 기전에 대해서는 앞으로 연구가 더 필요한 것으로 생각된다.

• 대한의생명과학회지
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• 제20권3호
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• pp.156-161
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• 2014

It has been reported that safflower seeds have preventive effects against osteoporosis. Recently, safflower buds (SB) were found to have more useful functional ingredients than safflower seeds. In the current study, we evaluated the anti-osteoporosis effects of SB diet in ovariectomized (OVX) rats. The rats were divided into five groups; sham operated group, OVX alone group, OVX plus $17{\beta}$-estradiol ($E^2$ $10{\mu}g/kg$, i.p.) and OVX plus SB diet feeding group (0.3% or 1%). Feeding of SB diet (0.3% or 1%) to OVX rats markedly increased bone mineral density (BMD) of femurs, compared to the OVX group. The OVX rats exhibited a marked increase in trabecular separation (Tb.Sp) and this change was inhibited by the feeding of SB diet, similar to that seen with OVX+E2 group. Moreover, feeding of SB diet to OVX rats decreased the markers of bone turnover, including osteocalcin and alkaline phosphatase (ALP). These results suggest that SB extract has a bone sparing effect in OVX-induced trabecular bone loss and prevents deterioration of bone microarchitecture by suppressing the rate of bone turnover. Therefore, SB may be useful for preserving bone mass and structure in estrogen deficient women with a potential role in reducing postmenopausal osteoporosis.