• 한국잠사곤충학회지
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• 제45권1호
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• pp.25-30
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• 2003

뇌신경세포주(PC12)에 백강잠의 분획물(조추출물)을 10 $\mu\textrm{g}$/ml 농도로 처리한 결과 헥산분획층과 부탄올분획층에서 뇌신경세포성장을 촉진하는 활성을 나타내어 헥산분획물로부터 화합물 4종(Fig.2: compound 1-4)과 부탄올분획물로부터 화합물 5종(Fig.2: compound 5-9)을 분리하여 각각의 화합물에 대하여도 검색한 결과 모두 뇌신경세포주(PC12)에 뇌신경세포 성장 촉진효과를 나타냈으며 특히 이들중 3종(4E, 6E, 2S, 3R)-2-N-Eicosanoy1-4,6-tetrade-casphingadienine,(4E, 6E, 2S, 3R)-2-N-Docosanoyl-4,6-tetradecasphingadienine, Urea)이 화합물은 NGF보다 우수한 활성을 나타냈다. 또한 헥산분획물로부터 분리한 4종의 스핑고신 유도체는 천연에서 처음 보고되는 물질이다. 이러한 결과로부터 백강잠 유래의 유효성분을 함유하는 추출물은 뇌졸중, 뇌허혈, 파킨슨, 노인성치매 및 헌팅턴질환을 포함하는 뇌질환 예방 및 치료를 위한 약학적 제제로 활용할 수 있을 것으로 기대된다.

• 대한약리학회지
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• 제29권2호
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• pp.165-174
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• 1993

칼슘이온은 신경섬유 성장의 중요한 조절인자이나 그 정확한 작용기전은 불명확하다. 세포골격 단백은 in vivo 및 in vitro에서 칼슘의존성 단백분해효소(칼파인)에 의해 신속히 분해되므로, 칼슘이온에 의한 신경섬유의 퇴행에 있어서 칼파인의 관련성을 추구하기위하여, 배양된 해마신경세포에서 칼슘이온 ionophore인 A23187에 의한 신경섬유의 성장억제가 칼파인의 억제제인 EST 및 MDL 28170에 의해 차단되는지를 조사하였다. A23187은 100nM의 농도에서 축삭에는 영향이 없이 수상돌기의 퇴행을 유발하였으나, 300 nM의 농도에서는 축삭의 성장을 억제하였다. EST(5 혹은 20 uM) 및 MDL 28170(20 uM)은 100 nM A23187의 수상돌기에 대한 작용과 300 nM A23187의 축삭에 대한 작용을 효과적으로 차단하였다. EST는 A23187에의한 세포내 칼슘이온의 증가를 차단하지 못하였다. 이상의 결과는 해마추상체세포에서 칼슘에 의한 신경섬유의 퇴행이 칼파인에 의해 매개됨을 시사한다.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 1996년도 추계 학술대회 및 정기총회
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• pp.61.2-62
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• 1996

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.229.2-229
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• 1998

No Abstract, See Full Text

• 한국당과학회:학술대회논문집
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• 한국당과학회 2006년도 제1회 연례학술대회
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• pp.47-47
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• 2006

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
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• pp.189.2-189
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• 2000

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.181.1-181
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• 1999

No Abstract, See Full Text

• Food Science and Biotechnology
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• 제15권5호
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• pp.735-738
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• 2006

We examined the neuroprotective and neurotrophic effects of genipin fractionated from gardenia (Gardenia jasminoides Ellis) originating from Korea. The neurotrophic effects of the genipin containing fraction was evaluated by microscopically monitoring its potency to induce neurite outgrowth in PC12h cells. The genipin containing fraction from Korean gardenia promoted neurite outgrowth in PC12h cells in this study, similar to previously reported effects by Wako Chemical, Japan. When cells were treated with the genipin containing fraction prior to ${\beta}$-amyloid peptide treatment (active domain of A peptide 25-35 treated), toxicity was significantly diminished (p<0.0l). These results suggest that genipin prepared from Korean gardenia might potentially be used as a precautionary agent in neurodegenerative disease, such as Alzheimer's disease, etc.

• 한국초전도학회:학술대회논문집
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• 한국초전도학회 2000년도 High Temperature Superconductivity Vol.X
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• pp.51-55
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• 2000

Using modified melt-textured grown targets, YBa$_2$Cu$_3$O$_{7-{\delta}}$ thin films were prepared by pulsed laser deposition technique at the laser energy density from 1 J/cm$_2$ to 4 J/cm$_2$. All the films showed c-axis preferred orientations, however, a-axis outgrowths on the film surface were considerably increased with an increase of the laser energy density. To examine the origin of the a-axis outgrowth formation, the microstructures of films deposited at 2 J/cm$_2$ and 4 J/cm$_2$ were investigated using X-ray diffraction, transmission electron microscopy, and high-resolution electron microscopy. It was shown that a significant number of Y$_2$O$_3$ inclusions were formed during the growth of c-axis oriented films at 4 J/cm$_2$. These inclusions formed nucleation sites for the a-axis outgrowths. It is considered that, due to the unstable growth conditions with a high flux density of incident vapor species and the strain induced by the surrounding c-axis films, the Y$_2$O$_3$ inclusions would prefer the nucleation of α-axis grains.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1490-1495
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• 2009

The development of nanotechnology has penetrated the fields of biology and medicine, resulting in remarkable applications for tissue regeneration. In order to apply this technology to tissue engineering, we have developed nano-scaled 3D scaffolds consisting of growth factor-loaded heparin/poly(l-lysine) nanoparticles (NPs) attached to the surface of polymeric micro spheres via polyionic complex methods. Growth factor-loaded NPs were simply produced as polyelectrolyte complexes with diameters of 100-200 nm. They were then coated onto positively charged poly(lactic-co-glycolic acid) (PLGA) pretreated with polyethyleneimine to enable cell adhesion, proliferation, and stimulation of neurite outgrowth. Propidium iodide staining and $\beta$-tubulin analysis revealed that neuronal PC12 cells proliferated extensively, expressed significant amounts of b-tubulin, and showed well-structured neurite outgrowth on polymeric microspheres by stimulation with growth factors. These results suggest that cellular adhesion and biological functionality on prepared PLGA microspheres enabled terminal differentiation of neuronal cells.