Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

A useful material isolation from the Bombycis corpus (Beauveria bassiana) growing of the silkworm, Bombyx mori L.

백강잠(Bombysis corpus)으로부터 유용물질 분리

  • 정이연 (농업과학기술원 잠사곤충부) ;
  • 남성희 (농업과학기술원 잠사곤충부) ;
  • 홍인표 (농업과학기술원 잠사곤충부) ;
  • 유승헌 (충남대학 농과대학) ;
  • 권학철 (성균관대학교 약학대학) ;
  • 이강노 (성균관대학교 약학대학)
  • Published : 2003.06.01
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Abstract

This study was carried out to investigate active constituents of Bombysis corpus on the neurite outgrowth from PC 12 cells led to isolate three new and a known sphingolipids from the n-hexane soluble portion and five amines from the butanol soluble portion of its methanol extract. On the basis of spectroscopic data, their structures have been elucidated as (4E,6E,2S,3R)-2-N-eicosanoyl-4,6-tetradecasphingadienine, (4E,2S,3R)-2-N-eicosanoyl-4-tetradecasphingenine,(4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine,(4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine,(4E,2S,3R)-2-N-octadecanoyl-4-tetradecasphingenine, 1,7-dimethyl-xanthine, uracil, urea, betaine and tyrosine, respectively. The neurite outgrowth activities of these compounds were examined in PC12 cells by measuring the length of neurites. These compounds promoted neurite outgrowth in PC12 cells significantly.

뇌신경세포주(PC12)에 백강잠의 분획물(조추출물)을 10 $\mu\textrm{g}$/ml 농도로 처리한 결과 헥산분획층과 부탄올분획층에서 뇌신경세포성장을 촉진하는 활성을 나타내어 헥산분획물로부터 화합물 4종(Fig.2: compound 1-4)과 부탄올분획물로부터 화합물 5종(Fig.2: compound 5-9)을 분리하여 각각의 화합물에 대하여도 검색한 결과 모두 뇌신경세포주(PC12)에 뇌신경세포 성장 촉진효과를 나타냈으며 특히 이들중 3종(4E, 6E, 2S, 3R)-2-N-Eicosanoy1-4,6-tetrade-casphingadienine,(4E, 6E, 2S, 3R)-2-N-Docosanoyl-4,6-tetradecasphingadienine, Urea)이 화합물은 NGF보다 우수한 활성을 나타냈다. 또한 헥산분획물로부터 분리한 4종의 스핑고신 유도체는 천연에서 처음 보고되는 물질이다. 이러한 결과로부터 백강잠 유래의 유효성분을 함유하는 추출물은 뇌졸중, 뇌허혈, 파킨슨, 노인성치매 및 헌팅턴질환을 포함하는 뇌질환 예방 및 치료를 위한 약학적 제제로 활용할 수 있을 것으로 기대된다.

Keywords

References

  1. J. Am. Chem. Soc. v.65 Raman spectra of amino acids and related compounds. Ⅵ.Sarcosine, ethanolamine, choline, betaine and betaine derivatives Edsall,J.T. https://doi.org/10.1021/ja01249a029
  2. Nature v.329 Ameliaoration of cholingergic neuron atrophy and spatial memory impairment in aged rats by nerve growth factors Fisch,W.;Wictorin,K.;Gage,F.H. https://doi.org/10.1038/329065a0
  3. J. Neurobiol. v.25 Neurotrophic factor therapy for nerve system degenerative diseases Hefti,F. https://doi.org/10.1002/neu.480251109
  4. Liebigs Annalen Isolation and structure of cerebrosides from the starfish Stellaster equestris Higuchi,R.;Harano,Y.;Mitsuyuki,M.;Isobe,R.;Yamada,K.;Miyamoto,T.;Komori,T.
  5. Chemical & Pharmaceutical Bulletin v.45 Structural elucidation of glycosphingolipids by collision-induced dissociation of sodium ion complex Isobe,R.;Inagaki,M.;Harano,Y.;Sakiyama,H.;Higuchi,R. https://doi.org/10.1248/cpb.45.1611
  6. Tetrahedron Letters v.40 Nanogram scale absolute configurational assignment of ceramides by circular dichroism Jiang,H.;Huang,X.;Nakanishi,K.;Berova,N. https://doi.org/10.1016/S0040-4039(99)01581-6
  7. Cognit. Disord. v.9 Intracerebroventricular infusion of nerve growth factor in three patients with Alzheimer's disease Jonhagen,M.E.;Nordberg,A.;Amberla,K.
  8. Journal of Lipid Research v.13 Identification and quantitation of free ceramides in human platelets Krivit,W.;Hammarstrom,S.
  9. Yakhak Hoeji v.43 Cytotoxic constituents of Bombycis corpus Kwon,H.C.;Moon,H.I.;Choi,S.H.;Lee,J.O.;Cho,S.Y.;Jung,I.Y.;Kim,S.Y.Lee,K.R.
  10. Yakhak Hoeji v.44 Cytotoxic cyclodepsipepides of Bombycis corpus 101A Kwon,H.C.;Bang,E.J.;Choi,S.U.;Lee,W.C.;Cho,S.Y.;Jung,I.Y.;Kim,S.Y.;Lee,K.R.
  11. 중약대사전(완역판) 김창민;신민교;안덕균;이경순
  12. J. Am. Chem. Soc. v.79 The infrared spectra of some amino acids Leifer,A.;Lippincott,E.R. https://doi.org/10.1021/ja01576a006
  13. Biol. Pharm. Bull. v.22 Enhancement of the nerve growth factor-mediated neurite outgrowth from PC12D cells by Chinese and Paraguayan medicinal plants Li,P.;Matsunaga,K.;Ohizumi,Y. https://doi.org/10.1248/bpb.22.752
  14. Trends Neurosci. v.17 Neurotrophins and brain insults Lindavall,O.;Kokaia,Z.;Bengzon,J.;Elmer,E.;Kokaia,M. https://doi.org/10.1016/0166-2236(94)90139-2
  15. Tetrahedron v.41 Synthesis of (4E,8E, 2S,3R, 2'R)-N-2'-hydroxyhexadecanoyl-9 methyl-4,8-sphingadienine. The ceramide portion of the fruiting-inducing cerebroside in a basidiomycete Schizophyllum commune and its(2R,3S)-isomer Mori,K.;Fumaki,Y. https://doi.org/10.1016/S0040-4020(01)96632-3
  16. Chem. Pharm. Bull. v.24 ¹³C-Nuclear magnetic resonance and raman spectroscopic studies on ionization and mercury complex of ergothioneine Motohashi,N.;Mori,I.;Sugiura,Y. https://doi.org/10.1248/cpb.24.1737
  17. Biochemi. Biophys. Res. Commu. v.751 Selegiline and Desmethylselegiline Stimulate NGF, BDNF, and GDNF Synthesis in Cultured Mouse Astrocytes Mizuta,I.;Ohta,M.;Ohta,K.;Nishimura,M.;Mizuta,E.;Hayashi,K.;Kuno,S.