• Journal of Plant Biology
• /
• v.32 no.4
• /
• pp.235-245
• /
• 1989

The contents and the compositions of total free amino acids were investigated in the rice(Oryza sativa L. cv. Chuncheong) seedlings under low temperatures. Activities of some enzymes associated with the markedly changed amino acid content were also investigaetd. Under low temperature, the contents of soluble protein and the total free amino acids increased, while the content of total nitrogen decreased. Although asparagine＋glycine were the most abundant amino acid speceis in the rice seedlings at the control temeprature, low temperature treatment for 3days brought about the decrease in their amount to about 60% level of the control plants. On the other hand, alanine showed the highest increase in the content among all the free amino acids, though glutamine, proline, asprtic acid, valine and tyrosine also increased after low temperature treatment. To eludicate the decrease of asparagine＋glycine level under low temperature, the activities of asparagine aminotransferase and asparaginase which metabolize asparagine were investigated in the rice seedlings under low temperature. The activity of asparaginase increased markedly, while that of asparagine aminotransferase decreased under low temperatures. Therefore, it was suggested that asparaginase metabolizes asparagine predominatly in the rice seedlings under low temperatures.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.11a
• /
• pp.387-387
• /
• 2005

• Journal of Plant Biotechnology
• /
• v.4 no.1
• /
• pp.39-44
• /
• 2002

The fast-migrating cationic peroxidase isozyme, named RC3, was purified from rice (Oryza sativa cv. Nak-Dong) callus. Purification of the enzyme was accomplished by ammonium sulfate fractionation, CM-cellulose ionexchange chromatography, and Sephacryl S-100 gel filtration. The molecular mass of the enzyme was about 34 KDa as determined by SDS-PACE and 38 KDa by Sephacryl-100 gel filtration. The pI value of the enzyme was 8.9. Antiserum against RC3 was raised in rabbits, and anti RC3 antiserum reacted with RC3 isozyme by Ouchterlony double immunodiffusion. The optimum pHs and Km values of the enzyme for various substrates were determined. Kinetic studies with various substrates showed that RC3 had very low Km value of 0.01 mM for ferulic acid and ascorbic acid. However, the enzyme did not use esculetin as a substrate.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.47 no.4
• /
• pp.301-304
• /
• 2002

The variation of alternative and cytochrome respiration during ripening in rice leaves (Oryza sativa L. cv. Takanari) was examined. The capacity of both respiration pathway was measured by inhibitor titration method using gas-phase oxygen electrodes. The alternative respiration rate decreased from 31.3% of the total respiration rate at around heading to 11.7% at 34 days after heading in the first fully expanded leaf from the top, and further to 6.4 % at 34 days after heading in the fourth leaf from the top. In contrast, the proportion of cytochrome respiration to total respiration increased with leaf senescence. The possible cause of alternative respiration as either an increase in inefficient respiration or a decrease in growth efficiency during ripening was discussed.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2004.10a
• /
• pp.78-79
• /
• 2004

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2004.04a
• /
• pp.134-135
• /
• 2004

• Proceedings of the Korean Society of Plant Pathology Conference
• /
• 2003.10a
• /
• pp.83.2-83
• /
• 2003

A full length cDNA, OsLEUZIP, encoding leucine zipper containing protein from rice EST of rice (0ryza sativa L. cv. Dongjin) treated Xanthomonas oryzae pv. oryzae 10331. OsLEUZIP contains 1,227 bp nucleotides and encodes a protein of 408 amino acid residues with predicted molecular weight of 47,229 Da. The deduced amino acid sequence of OsLEUZIP has consensus sequence of leucine zipper from PROSITE (PDOC00029), L-X(6)-L-X(6)-L-X(6) -L. OsLEUZIP gene were preferentially induced in rice during incompatible interaction with Xanthomonas oryzae pv. oryzae 10331 and Pyracuraria grisea KJ-301. Expression of OsLEUZIP gene was also induced by treatment of abiotics such as ethephon and ABA. Our data represented in this study suggesting that OsLEUZIP gene may play an important role in the rice defense-related. Further studies of this gene, overexpression in rice, yeast-two hybrid assay, electrophoretic mobility shift assay and northern blot analyses of transgenic plant, would be useful to elucidate the role of the OsLEUZIP gene in defense responses of rice.

• KSBB Journal
• /
• v.10 no.1
• /
• pp.23-29
• /
• 1995

Calli were induced from leaf base region of germinated rice(Oryza sativa L. cv. Nakdong) with high frequency of up to 65% on LS medium supplemented with $2.5mg/{\ell}2$, 4-D in the dark at $27^{\circ}C$. Embryogenic calli of pale yellow, globular type were selected and used for the initiation of cell suspension cultures in AA2 liquid medium with $2mg/\ell$ 2,4-D, 0.2mg/$\ell$ kinetin arid $0.1mg/\ell$ GA3. Protoplasts were isolated from the embryogenic cell suspensions after 4 months of culture and then were electroporated with 400V/cm for 1 msec. Electroporated protoplasts divided with plating efficiency of 1.1% on PCM liquid medium supplemented with $2.5mg/\ell$ 2, 4-D, $0.1mg/\ell$ kinetin and 10mM proline. The protoplasts-derived microcalli were cultured on $0.2{\mu}m$ membrane fitter placed onto LS2.5 solid medium containing fine suspension cells as a feeder cells, for 2 weeks in the dark at $27^{\circ}C$. After an additional 2 weeks of culture under fluorescent light of $30{\pm}/3{\mu}E$.m^{-2}S^{-1}, yellow calli of 2mm diameter were transferred to regeneration medium. Shoots were produced from the green spot of protoplasts-derived calli and plants were regenerated form protoplast-derived green calli with frequencies of 11∼33%.

• Korean Journal of Weed Science
• /
• v.18 no.1
• /
• pp.20-27
• /
• 1998

This experiments were conducted to investigate the effects of fertilizer for herbicides phytotoxicity of rice(Oryza sativa L. japonica cv Dongjin). The shoot and root growth of rice were inhibited more by pyrazosulfuron-ethyl or pyrazosulfuronethyl+molinate than bensulfuron methyl or bensulfuron methyl+dimepiperate application in nitrogen or nitrogen mixed solution. In phosphate or phosphate mixed solution, rice growth were inhibited more by bensulfuron methyl or bensulfuron methyl+dimepiperate than pyrazosulfuron-ethyl or pyrazosulfuron-ethyl+molinate application. In solution mixed with nitrogen and phosphate or fertilizer three elements, rice shoots were more. inhibited by pyrazosulfuron-ethyl or pyrazosulfuron-ethyl+molinate treatments, roots were inhibited more relatively by bensulfuron methyl or bensulfuron methyl+dimepiperate treatments. In all fertilizer solutions, rice plant heights were reduced by dimepiperate and molinate applications, but root growth was reduced only by nitrogen and phosphate mixed solution. Rice growth in sulfonylurea or their mixed herbicide application were more inhibited in high :nitrogen concentration arid by phosphate exclusion than by nitrogen exclusion culture.

• Journal of Wetlands Research
• /
• v.16 no.4
• /
• pp.363-370
• /
• 2014

We grew seedlings of Saechucheongbyeo and Odaebyeo of rice cultivars that are cultivated dominantly in the northern and middle regions of Korea under control(ambient condition), ambient $CO_2$ concentration+elevated temperature, and elevated $CO_2$ concentration+elevated temperature in order to study how growth responses and crop yield of major rice of Korea change as the global warming proceeds and compared the results. Aboveground biomass, belowground biomass, total biomass, and panicles weight per individual and ripended grain rate of cv. Saechucheongbyeo were the highest under control, but those of cv. Odaebyeo were the highest under elevated $CO_2$ concentration+elevated temperature. There was no difference in the number of panicles per individual of cv. Saechucheongbyeo and cv. Odaebyeo in these experiments. There was no difference in the number of grains per panicle of cv. Saechucheongbyeo among three environmental gradients, but that of cv. Odaebyeo was the highest under elevated $CO_2$ concentration+elevated temperature. Weight of a grain of cv. Saechucheongbyeo was highest under elevated $CO_2$ concentration+elevated temperature, but that of cv. Odaebyeo was the higher under ambient $CO_2$ concentration+elevated temperature and elevated $CO_2$ concentration+elevated temperature. Thus, if global warming continues in Korea, selection of rice cultivation varieties must be chosen carefully for commendation.