• 제목/요약/키워드: Oriental-western medicine treatment

검색결과 1,039건 처리시간 0.032초

가임기(可姙期) 여성(女性)의 임신경험(姙娠經驗)에 따른 양생수준(養生水準)과 난임변증(難姙辨證) (Yangsaeng Level and Pattern Identification of Subfertility in Childbearing Aged Women according to the Pregnancy Experience)

  • 김영희;박금숙;이경완;진은영;정헌영
    • 대한한의학원전학회지
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    • 제28권3호
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    • pp.107-124
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    • 2015
  • Objectives : The purpose of this study is to identify Yangsaeng level and pattern identification of subfertility in childbearing aged women according to the pregnancy experience. Methods : Data was collected by structured questionnaire distributed to 217 women(125 women experienced pregnancy, 92 subfertile women) in I city from Nov. to Dec. 2014. For data analysis, Chi-square test, Fisher's exact test, t-test and Pearson's correlation coefficients were performed using SPSS version 19.0. Results : Drinking rate and stress level of subfertile group were higher than pregnancy experience group. Subfertile women group have more disease related to reproductive system, irregularities of the menstrual cycle and dysmenorrhea than pregnancy experience group. Subfertile women showed lower scores for Yangsaeng level but higher scores for all types of pattern identification of subfertility more than those in the women experiencing pregnancy. Conclusions : The results of this study indicate that reproductive health program for the childbearing aged women is necessary before serviced by western or oriental medical treatment for subinfertile(infertile) women. These results can be used for a program based on oriental medicine in order to improve women's health.

Ethanolic Extract of Pancake Mixture Powder Supplemented with Helianthus tuberosus Enhances Antidiabetic Effects via Inhibiting Inflammatory Mediator NO Production

  • Lee, Kyoung-Dong;Sun, Hyeon-Jin;Lee, Mina;Chun, Jiyeon;Shin, Tai-Sun;Choi, Kap Seong;Shim, Sun-Yup
    • 한국미생물·생명공학회지
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    • 제50권2호
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    • pp.228-234
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    • 2022
  • Helianthus tuberosus is perennial plant as Compositae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixture powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro α-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3β activation in L6 cells. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory effects of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.

뇌 신경교세포에서 가미보양환오탕(加味補陽還五湯) 분획물의 항염증 효과 비교 연구 (Anti-inflammatory Effects of Different Fractions Isolated from Modified Boyanghwano-tang Extract in LPS-stimulated Microglial Cells)

  • 손혜영;박용기
    • 대한본초학회지
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    • 제24권4호
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    • pp.173-179
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    • 2009
  • Objectives : In this study, the effects of different fractions isolated from modified Boyanghwanotang(mBHT) extract on LPS-induced inflammation in BV2 microglial cells were investigated. Methods : mBHT was extracted with water, and then fractionated with n-hexane, methylene chloride, ethylacetate and n-butanol. BV2 cells, a mouse microglia line were incubated with different concentrations of each fraction of mBHT for 30 min, and then stimulated with LPS for 24 h. Cell toxicity was determined by MTT assay. The concentration of nitric oxide (NO) was measured in culture medium by Griess reagent assay. The expression of inducible nitric oxide synthease (iNOS) protein was determined by Western blot. Results : Four fractions of mBHT were significantly inhibited LPS-induced NO productions in BV2 cells in a dose-dependent manner. The methylene chloride fraction of mBHT was most strongly inhibited the NO production compared with those of the others. The methylene chloride fraction of mBHT was also suppressed LPS-induced iNOS expression comparison of other fractions at same concentration ($50\;{\mu}g/ml$) in BV2 cells. Conclusions : The results showed that the methylene chloride fraction of mBHT may have an strong anti-inflammatory property through the inhibition of NO production and iNOS expression in activated microglia, and could a therapeutic potential for the treatment of various brain inflammatory diseases.

강활의 RAW264.7 세포에서 LPS에 의해 유도되는 염증물질 생성에 대한 효과 (The Effects of Different Extracts of Ostericum koreanum on the Production of Inflammatory Mediators in LPS-stimulated RAW264.7 Cells)

  • 김창민;박용기
    • 대한본초학회지
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    • 제24권1호
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    • pp.169-178
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    • 2009
  • Objectives : In this study, the effects of ethylacetate extract of Ostericum koreanum on inflammation in RAW264.7 cells were investigated. Methods : Dried roots of Ostericum koreanum was extracted with 80% methanol for 24 h, and then fractionated with n-butanol, n-hexan and ethylacetate. RAW264.7 cells, a mouse macrophage line were incubated with different concentrations of the extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO) and prostaglandin E2 ($PGE_2$) were measured by Griess assay and enzyme immunoassay (EIA), respectively. The expressions of inducible nitric oxide synthease (iNOS) and cyclooxyganase (COX) -2 mRNA and protein were determined by RT-PCR and Western blot. Results : The methanol extract of Ostericum koreanuman and its fractions were significantly inhibited the NO and PGE2 productions in LPS-stimulated RAW264.7 cells. Among the fractions of Ostericum koreanuman the ethylacetate fraction was more strongly inhibited NO and $PGE_2$ productions compared with other fractions. The ethylacetate fraction was also suppressed LPS-induced mRNA expressions of iNOS and its protein levels in RAW264.7 cells. Conclusions : This study suggests that the ethylacetate fraction of Ostericum koreanum may have an anti-inflammatory property through suppressing inflammatory mediator productions in activated macrophages, suggesting have a therapeutic potential for the treatment of various inflammatory diseases.

성인병 환자들의 건강과 관련된 자기조절에 대한 현상학적 연구 (Phenomenological Approach of Self Regulation Related to Health of patients with Adult Disease)

  • 김숙영
    • 대한간호학회지
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    • 제25권3호
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    • pp.562-580
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    • 1995
  • This research was an attempt to make it possible to provide nursing care and health education meet- ing the need not of care givers but of patients by identifying the nature of patients' self regulation experiences. The specific objective of this study was : 1) to explore self regulation experiences of patients with adult disease. The phenomenological approach in qualitative studies is used to serve this purpose. Colaizzi's method is used for the phenomenological analysis of the data in this study, which were collected from 25 patients hospitalized in the internal medical ward and oriental medical ward of a Seoul hospital, suffering from adult disease such as hypertension, arteriosclerosis and diabetes mellitus. The research was conducted over a period of March to September, 1994. The investigator conducted participated observations and in-depth unstructured interviews which were audiotaped under the permission of patients. The investigator read the data repeatedly to identify and categorize significant statements, formulating meanings, themes and theme clusters. The result is categorized as follows : Self regulation activities, their barriers and predisposing factors of a disease. Thirteen theme clusters of self regulation activities related to health identified were. “maintaining diet regimen”, “maintaining exercise regimen”, “maintaining medication regimen”, “maintaining oriental medical regimen”, “maintaining health monitoring regimen”, “maintaining self effort”, “maintaining religious life”, “maintaining social sup-port systems”, “maintaining peaceful mind”, “maintaining moderation in life”, “maintaining sincere attitude in life”, “maintaining natural life”, and “maintaining folk remedy” This findings confirm the fact that self regulation is complicatedly and diversely influenced by oriental medicine and folk remedy, and Korean traditional ideas melted in Confucianism, Buddhism, Taoism and Shamanism, and modern medical care and western culture. Seven theme clusters of self regulation barriers identified were : "lack of knowledge and self aware-ness", "lack of social supports", "lack of awareness of need in continuous regimen and treatment", "dissatisfaction with hospital and health care provider", "lack of self management ", "lack of will to combat illness", and "overconfidence in folk remedy" Four theme clusters of predisposing factors of a disease were : "cumulation of stressors", "fatalism", "careless life style", and "family history". In conclusion, this. study will prove helpful not only in understanding clients in light of our traditional culture but also in providing them with the kind of nursing care and health education satisfying their demands and particularly cultural needs.

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HepG2 인체간암세포의 세포주기조절인자 발현에 미치는 sulforaphane의 영향 (Modulation of Cell Cycle Regulators by Sulforaphane in Human Mepatocarcinoma HepG2 Cells)

  • 배송자;김기영;유영현;최병태;최영현
    • 생명과학회지
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    • 제16권7호
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    • pp.1235-1242
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    • 2006
  • 브로콜리를 포함한 십자화과 식물에서 glucoraphanin의 가수분해를 통해 생성되는 isothiocyanate의 일종인 sulforaphane은 역학적 조사를 포함한 다양한 선행 연구에서 강력한 암예방 효과를 가지는 것으로 알려져 있다. 항암효과에 관한 최근 연구 결과에 따르면 sulforaphane은 다양한 인체암세포의 증식을 억제하고 apoptosis를 유발할 수 있는 것으로 알려지고 있으나, 정확한 분자생물학적 기전은 밝혀져 있지 않은 상태이다. 본 연구에서는 sulforaphane의 항암작용 기전을 조사하기 위하여 HepG2 인체간암세포의 증식에 미치는 sulforaphane의 영 향을 조사하였다. Sulforaphane의 처리에 의한 HepG2 세포의 증식억제 및 형태적 변형은 세포주기 G2/M arrest 및 apoptosis 유발과 밀접한 관련이 있음을 알 수 있었다. RT-PCR 및 Western blot 분석 결과, sulforaphane 처리에 의하여 cyclin A 및 cyclin B1, Cdc2의 발현이 단백질 수준에서 선택적으로 저하되었으며, 종양억제 유전자 p53 및 Cdk inhibitor p21의 발현은 전사 및 번역 수준에서 sulforaphane 처리 농도 의존적으로 증가되었다. Sulforaphane의 항암 기전을 규명하기 위해서는 더 많은 연구가 부가적으로 필요하겠지만, 본 연구의 결과들에 의하면 sulforaphane은 강력한 인체암세포의 증식 억제 및 항암작용이 있을 것을 시사하여 준다고 할 수 있다.

Effects of Korean Red Ginseng extract on tissue plasminogen activator and plasminogen activator inhibitor-1 expression in cultured rat primary astrocytes

  • Ko, Hyun Myung;Joo, So Hyun;Kim, Pitna;Park, Jin Hee;Kim, Hee Jin;Bahn, Geon Ho;Kim, Hahn Young;Lee, Jongmin;Han, Seol-Heui;Shin, Chan Young;Park, Seung Hwa
    • Journal of Ginseng Research
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    • 제37권4호
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    • pp.401-412
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    • 2013
  • Korean Red Ginseng (KRG) is an oriental herbal preparation obtained from Panax ginseng Meyer (Araliaceae). To expand our understanding of the action of KRG on central nervous system (CNS) function, we examined the effects of KRG on tissue plasminogen activator (tPA)/plasminogen activator inhibitor-1 (PAI-1) expression in rat primary astrocytes. KRG extract was treated in cultured rat primary astrocytes and neuron in a concentration range of 0.1 to 1.0 mg/mL and the expression of functional tPA/PAI-1 was examined by casein zymography, Western blot and reverse transcription-polymerase chain reaction. KRG extracts increased PAI-1 expression in rat primary astrocytes in a concentration dependent manner (0.1 to 1.0 mg/mL) without affecting the expression of tPA itself. Treatment of 1.0 mg/mL KRG increased PAI-1 protein expression in rat primary astrocytes to $319.3{\pm}65.9%$ as compared with control. The increased PAI-1 expression mediated the overall decrease in tPA activity in rat primary astrocytes. Due to the lack of PAI-1 expression in neuron, KRG did not affect tPA activity in neuron. KRG treatment induced a concentration dependent activation of PI3K, p38, ERK1/2, and JNK in rat primary astrocytes and treatment of PI3K or MAPK inhibitors such as LY294002, U0126, SB203580, and SP600125 (10 ${\mu}M$ each), significantly inhibited 1.0 mg/mL KRG-induced expression of PAI-1 and down-regulation of tPA activity in rat primary astrocytes. Furthermore, compound K but not other ginsenosides such as Rb1 and Rg1 induced PAI-1 expression. KRG-induced up-regulation of PAI-1 in astrocytes may play important role in the regulation of overall tPA activity in brain, which might underlie some of the beneficial effects of KRG on CNS such as neuroprotection in ischemia and brain damaging condition as well as prevention or recovery from addiction.

Rosuvastatin이 유도하는 ROS가 전립선암 PC-3 세포주의 세포사멸 유도에 미치는 영향 (Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells)

  • 최현덕;백종진;김상헌;유선녕;천성학;김영욱;남효원;김광연;안순철
    • 생명과학회지
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    • 제26권4호
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    • pp.398-405
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    • 2016
  • 3-Hydroxy-3-methylglutaryl coenzyme A 환원효소의 억제제로 알려진 statin은 고지혈증 치료제로 널리 사용되고 있고, 또한 다양한 암에서 항암효과를 나타낸다고 알려져 있다. 최근 연구에서는 reactive oxygen species (ROS)가 세포사멸 신호에 중요한 역할을 한다고 보고하였지만, rosuvastatin에 의한 ROS 생성의 정확한 기전은 아직 밝혀지지 않았다. 인간 전립선암 세포주인 PC-3 세포를 이용하여 rosuvastatin에 의한 세포사멸 경로를 확인하였다. 세포독성, 세포사멸과 ROS의 생성을 측정하기 위해서 MTT assay, annexin V/PI 염색과 DCFH-DA염색을 통해 flow cytometry에 의해 측정하였다. 본 연구의 결과에서, rosuvastatin은 농도와 시간 의존적으로 세포 생존율 감소와 세포형태변화를 확인할 수 있었다. Flow cytometry 분석을 통해 세포주기와 apoptosis를 확인하였고 Western blotting assay를 통하여 PARP와 procaspase-3가 감소되는 것을 통해 apoptosis를 재확인 할 수 있었다. 또한 rosuvastatin은 농도 의존적으로 ROS 생산을 증가하였고, ROS 생성 저해제인 N-acetylcysteine (NAC) 처리를 통해 ROS와 apoptosis가 회복되었다. 따라서 rosuvastatin이 ROS 생성을 통해 apoptosis를 유도한다는 것을 알 수 있었고, 이는 인간 전립선 암세포에 대한 항암치료제로서의 가능성을 제시한다.

Overexpression of Anti-apoptotic Molecules and Sax Translocation to Mitochondria by Pharbitis Nil Extracts in AGS

  • Ko Seong-Gyu
    • 동의생리병리학회지
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    • 제18권6호
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    • pp.1843-1849
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    • 2004
  • Conventional medicines have usually sorted to a number of treatments such asoperation, radiotherapy, and chemotherapy. The existing anti-cancer agents, designed to eradicate cancer cells, have strong toxicities, also with leading to harmful side effects. Recently, a number of researches on natural products have been actively carried out in efforts to develop new treatments that can decrease side effects or increase anti-cancer effects. We performed this study to understand the molecular basis underlying the antitumor effects of Pharbitis nil, and Plantago asiatica, which have been used for herbal medicinal treatments against cancers in East Asia. We analyzed the effects of these medicinal herbs on proliferation and on expression of cell growth/apoptosis related molecules, with using an AGS gastric cancer cell line. The treatment of Pharbitis nil dramatically reduced cell viabilities in a dose and time-dependent manner, but Plantago asiatica didn't. FACS analysis and Annexin V staining assay also showed that Pharbitis nil induce apoptotic cell death of AGS. Expression analyses via RT-PCR and Western blots revealed that Pharbitis nil didn't increase expression of the p53 and its downstream effector p21/sup wafl/, and that the both increased expression of apoptosis related Sax and cleavage of active caspase-3 protein. We also confirmed the translocation of Sax to mitochondria. Collectively, our data demonstrate that Pharbitis nilinduce growth inhibition and apoptosis of human gastric cancer cells, and these effects are correlated with down- and up-regulation of growth-regulating apoptotic and tumor suppressor genes, respectively.

Palmitate로 유발된 비알코올성 지방간 모델에 대한 KH청간탕(淸肝湯)의 효과 연구 (Effects of KHchunggan-tang on the Nonalcoholic Fatty Liver Disease in Palmitate-induced Cellular Model)

  • 한창우;이장훈
    • 대한한의학회지
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    • 제32권1호
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    • pp.109-120
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    • 2011
  • Objectives: The aim of this investigation was to evaluate the efficacy of KHchunggan-tang aqueous extract on the experimental nonalcoholic fatty liver disease(NAFLD) induced by palmitate. Materials and Methods: To generate a cellular model of NAFLD, we used HepG2 cells, a human hepatoma cell line, treated with 0.5 mM palmitate. By this cellular model, effects of KHchunggan-tang aqueous extract were evaluated. Intracellular lipid accumulation, free radical formation, and apoptosis were detected by Nile red staining, 2',7'-dichloroflourescin diacetate(H2DCF-DA), and 4',6-diamidino-2-phenylindole(DAPI)/propidium iodide(PI) staining, respectively. Some proteins related with NAFLD were determined by western blot. Results: Typical pathological features of NAFLD occurred in the cellular model. Palmitate increased the levels of intracellular lipid vacuoles, decreased cell viability, and increased apoptosis. Palmitate increased free radical formation and lipid peroxidation, too. However, KHchunggan-tang aqueous extract reduced palmitate-induced pathologic features, i.e. steatosis, free radical formation, and apoptosis. In addition, KHchunggan-tang aqueous extract suppressed palmitate-activated c-Jun N-terminal kinase(JNK) signaling, and SP600125, a JNK inhibitor, significantly reversed the palmitate-induced pathologic changes as KHchunggan-tang aqueous extract. It means that the signaling pathway other than JNK can be involved in the KHchunggan-tang mediated cellular protection of palmitate-treated Hep G2 cells. Conclusions: These results suggest that KHchunggan-tang aqueous extract has hepatoprotective effects on NAFLD with combined properties in cellular steatosis, ROS production, and cytoprotection, and thus may have valuable clinical applications for treatment of this chronic liver disease.