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http://dx.doi.org/10.5352/JLS.2016.26.4.398

Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells  

Choi, Hyeun Deok (Department of Microbiology & Immunology, Busan National University School of Medicine)
Baik, Jong Jin (Pusan National University School of Medicine)
Kim, Sang Hun (Department of Microbiology & Immunology, Busan National University School of Medicine)
Yu, Sun Nyoung (Department of Microbiology & Immunology, Busan National University School of Medicine)
Chun, Sung Hak (Department of Microbiology & Immunology, Busan National University School of Medicine)
Kim, Young Wook (Department of Microbiology & Immunology, Busan National University School of Medicine)
Nam, Hyo Won (Department of Microbiology & Immunology, Busan National University School of Medicine)
Kim, Kwang Youn (Department of Herbal Fomula, Medical Research Center (MRC-GHF), College of Oriental Medicine, Daegu Haany University)
Ahn, Soon Cheol (Department of Microbiology & Immunology, Busan National University School of Medicine)
Publication Information
Journal of Life Science / v.26, no.4, 2016 , pp. 398-405 More about this Journal
Abstract
Statins, the inhibitors of 3-hydroxy 3-methylglutaryl coenzyme A (HMG-CoA) reductase, are widely used in treatments of hypercholesterolemia and newly known as anti-cancer effect of various cancer cells. Recently, several studies suggested that reactive oxygen species (ROS) play a critical role on cell death signaling. However, mechanism of ROS by rosuvastatin is currently unclear. This study aimed to explore the molecular mechanism of apoptosis by rosuvastatin in human prostate cancer PC-3 cells. Cell viability and apoptosis-related protein expression were measured by MTT assay and western blotting, respectively. In addition, the levels of apoptosis and ROS were analyzed. The results showed that rosuvastatin dramatically reduced cell viability in a dose- and time-dependent manner. We confirmed that rosuvastatin induced apoptosis through reduction of procaspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) in PC-3 cells. In addition, rosuvastatin stimulated ROS production in a dose-dependent manner and pre-treatment with N-acetylcysteine (NAC), a ROS scavenger, significantly recovered rosuvastatin-induced ROS and apoptosis. Thus, we concluded that rosuvastain induces apoptosis through generation of ROS in human prostate cancer PC-3 cells and provides a promising approach to improve the efficacy of cancer therapy.
Keywords
Apoptosis; human prostate cancer PC-3 cells; N-acetylcysteine; reactive oxygen species; rosuvastatin;
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