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http://dx.doi.org/10.5352/JLS.2006.16.7.1235

Modulation of Cell Cycle Regulators by Sulforaphane in Human Mepatocarcinoma HepG2 Cells  

Bae, Song-Ja (Department of Food and Nutrition, Silla University and Marine Biotechnology Center for Bio-Functional Material Industries)
Kim, Gi-Young (Faculty of Applied Marine Science, Cheju National University)
Yoo, Young-Hyun (Department of Anatomy and Cell Biology, Dong-A University College of Medicine)
Choi, Byung-Tae (Anatomy and Dongeui University Oriental Medicine and Department of Biomaterial Control(BK21 program), Dongeui University Graduate School)
Choi, Yung-Hyun (Biochemistry, Dongeui University Oriental Medicine and Department of Biomateriqal Control(BK21 program), Dongeui University Graduate School)
Publication Information
Journal of Life Science / v.16, no.7, 2006 , pp. 1235-1242 More about this Journal
Abstract
Sulforaphane, an isothiocyanate derived from hydrolysis of glucoraphanin in broccoli and other cruciferous vegetables, was shown to induce phase II detoxification enzymes and inhibit chemically induced mammary tumors in rodents. Recently, sulforaphane is known to induce cell cycle arrest and apoptosis in human canter cells, however its molecular mechanisms are poorly understood. In tile present study, we demonstrated that sulforaphane acted to inhibit proliferation and induce morphological changes of human hepatocarcinoma HepG2 cells. Treatment of HepG2 cells with $10{\mu}M\;or\;15{\mu}M$ sulforaphane resulted in significant G2/M cell cycle arrest as determined by DNA flow cytometry. Moreover, $20{\mu}M$ sulforaphane significantly induced the population of sub-G1 cells suggesting that sulforaphane induced apoptosis. This anti-proliferative effect of sulforaphane was accompanied by a marked inhibition of ryclin A, cyclin 31 and Cdc2 protein. However, the levels of tumor suppressor p53 and Cdk inhibitor p21 mRNA and protein expression were significantly increased by sulforaphane treatment in a concentration-dependent manner. Although further studies are needed, the present work suggests that sulforaphane may be a potential rhemoprevetiveichemotherapeucc agent for the treatment of human cancer cells.
Keywords
Sulforaphane; HepG2 cells; cell cycle; p53; p21;
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