• Korean Journal of Medicinal Crop Science
• /
• v.27 no.2
• /
• pp.115-125
• /
• 2019

Background: Glycyrrhiza radix (licorice root) have been used as an oriental medicine material for long time, and its protective effects on oxidative stress, inflammation and cognition deficit have been recently reported. However, the cultivation of Glycyrrhiza species as medicinal crops is associated with some problems such as low productivity and early leaf fall, etc. To resolve this problems, Glycyrrhiza cultivars have been developed by direct hybridization of each Glycyrrhiza species by Korean researchers. The present study was conducted to compare the Glycyrrhiza cultivar radix (Dagam, Sinwongam and Wongam) for their anti-oxidation, anti-inflammation, and cognition improvement effects and levels of liquiritin, isoliquiritigenin and licochalcone in order to select an excellent cultivar as a material resource. Methods and Results: For evaluating the inhibitory efficacies of the Glycyrrhiza cultivar extracts on oxidative stress and inflammation in BV2 cells, we measured their reactive oxygen species (ROS) production and nitric oxide (NO) release after treating them with lipopolysccharide. The scavenging activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxynitrite ($NOO^-$) radicals were evaluated. Cell proliferation and N-methyl-D-aspartate receptor (NMDAR) inhibition were analyzed. The total phenol, liquiritin, isoliquiritigenin and licochalcone A content in the extracts of the three culivars were quantified. Furthermore, the correlation coefficient between the activities and contents of total phenol, liquiritin, isoliquiritigenin and licochalcone A were also calculated. The results indicated that Sinwongam exhibited potent anti-oxidant, anti-inflammatory and NMDAR inhibititory activities. Sinwongam also showed higher total phenol and licochalcone A contents than the other cultivars. Among the three cultivars, Dagam exhibited a positive effects on NO release inhibition, cell proliferation and contents of liquiritin and isoliquiritigenin. Conclusions: Sinwongam is expected to be the most useful resource as a functional material for anti-oxidation/anti-inflammation and cognition improvement among the three studied licorice cultivars.

• Journal of Ginseng Culture
• /
• v.1
• /
• pp.1-10
• /
• 2019

The purpose of this study is to investigate the changes of ginseng properties through bibliographical records. The cultivation of Korean wild simulated ginseng (mountain cultivated ginseng) started from the Goryeo Dynasty around A.D. 1000. Thereafter, from A.D. 1500 to A.D. 1600, ginseng cultivation technology was established in the Joseon Dynasty. At this time, the ginseng was begun to grow in the field near the house, and it is recorded as Gasam (field cultivated ginseng). The Jung-Jo-Sil-Rok (A.D. 1790) states that Gasam cultivation has expanded to the whole country. The properties of ginseng described in almost all oriental medicine books from A.D. 250 to A.D. 1600 were a slightly cold. However, after A.D. 1600, it is said to be slightly warm or warm. Considering from this record, the slightly cold ginseng before A.D. 1600 can be recognized as the properties of wild ginseng or wild simulated ginseng, and the slightly warm ginseng after A.D. 1600 can be recognized as Gasam properties.

• The Journal of the Korea Contents Association
• /
• v.21 no.10
• /
• pp.774-787
• /
• 2021

This thesis is a follow-up study on HwangJeNaeGyeogSoMun(SoMun). Its purpose is the usage of '心' letter used in HwangjenaegyeogYoungChu(YoungChu). The original manuscript of this study was the Hu's Gulin Sanctum of YoungChu. It was conducted by a literature review. Typically, the word '心' means a tangible heart and an intangible mind in the same form. Therefore, in order to understand the contents of the YoungChu, which provides the basis for the basic ideology related to health care, meditation, GiGong training, yoga, practice and oriental medicine, it is necessary to understand the meaning of the word '心' letter. The results of this study are as follows. First, it means human heart. Second, it means the human chest. Third, it means mind such as angry, joy sad, fear and so on. Fourth, it means the transcendent concept like spiritual enlightenment. Fifth, it means the pericardium. Sixth, it means logical thinking. Seventh, it means center or core, Eighth, it means the name of the constellation in the eastern sky of ancient Asia. Ninth, it can be classified into the inside. It can be used as a basic data to understand the contents of YoungChu related to various categories. The limitation of it is that the classification of the '心' letter may be different from the researchers' perspective.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.10a
• /
• pp.92-92
• /
• 2019

Excessive or chronic inflammation contributes to the pathogenesis of many inflammatory diseases such as sepsis, rheumatoid arthritis, and ulcerative colitis. Hibiscus syriacus L. has been used as a medicinal plant in many Asian countries, even though its anti-inflammatory activity has been unclear. Therefore, we investigated the anti-inflammatory effect of anthocyanin fractions from the H. syriacus L. varieties Pulsae (PS) on the lipopolysaccharide (LPS)-induced expression of proinflammatory mediators and cytokines in RAW264.7 macrophages. PS suppressed LPS-induced nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) secretion concomitant with downregulation of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Furthermore, PS inhibited the production of proinflammatory cytokines such as tumor necrosis factor-alpha ($TNF-{\alpha}$), interleukin-6 (IL-6), and IL-12 in LPS-stimulated RAW264.7 macrophages. Further study showed that PS significantly decreased LPS-induced nuclear translocation of the nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) subunits, p65 and p50. Molecular docking data showed that many anthocyanins from PS fit into the hydrophobic pocket of MD2 and bound to Toll-like receptor 4 (TLR4), indicating that PS inhibits the TLR4-MD2-mediated inflammatory signaling pathway. Especially, apigenin-7-O-glucoside most powerfully bound to MD2 and TLR4 through LYS122, LYS122, and SER127 at a distance of $2.205{\AA}$, $3.098{\AA}$, and $2.844{\AA}$ and SER441 at a distance of $2.873{\AA}$ (docking score: -8.4) through hydrogen bonding, respectively. Additionally, PS inhibited LPS-induced TLR4 dimerization/expression on the cell surface, which consequently decreased MyD88 recruitment and IRAK4 phosphorylation. PS completely blocked LPS-mediated mortality in zebrafish larvae by diminishing the recruitment of neutrophil and macrophages accompanied by low levels of proinflammatory cytokines. Taken together, our results indicate that PS attenuates LPS-mediated inflammation in both in vitro and in vivo by blocking the TLR4/MD2-MyD88/IRAK4-$NF-{\kappa}B$ axis. Therefore, PS might be used as a novel modulatory candidate for effective treatment of LPS-mediated inflammatory diseases.

• Journal of the Korean Applied Science and Technology
• /
• v.38 no.3
• /
• pp.870-882
• /
• 2021

Coptis chinensis has been used in the treatment of various diseases such as soothing, anti-inflammation, antimicrobial and antipyretic in oriental traditional medicine. In this study, we investigated the effect of hot water extract of Coptis chinensis(CCW) on skin barrier and inflammation-related factors in UVB and TNF-α/IFN-γ-induced HaCaT cells and evaluated its potential as a moisturizing and anti-inflammatory material. Based on result, the amount of HA (Hyaluronic acid) production and protein and mRNA expression of filaggrin were measured. In TNF-α/IFN-γ-induced HaCaT cells, CCW increased the amount of HA production in a concentration-dependent manner. In the measurement of protein and mRNA expression of filaggrin, the expression rate increased as the concentration of CCW increased. In UVB-induced HaCaT cells, CCW decreased the production of ROS and showed significant results with EGCG ((-)-epigallocatechin-3-gallate), a positive control. In addition, CCW inhibited the expression of inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-8 in TNF-α/IFN-γ-induced HaCaT cells. It was confirmed that the protein and mRNA expression of COX-2, a major factor in skin inflammation, was decreased in a concentration-dependent manner. These results suggest that hot water extract from Coptis chinensis can be used as a cosmetic material having a moisturizing and anti-inflammatory effect.

• Journal of Korea Society of Industrial Information Systems
• /
• v.25 no.6
• /
• pp.47-54
• /
• 2020

The acupressure is a treatment that applies pressure to certain parts of the body and has been mainly used for pain relief in the field of oriental medicine. However, the treatment effect is often different depending on the practitioner's ability, experience, and physical strength, so standardized acupressure is needed. In this regard, the equipment is being released, but this is mainly a rolling massage method, which reduces energy concentration and poses a risk of injury. Therefore, in this study, a device that provides vertical acupressure based on variable bogie (wheel truck) was implemented. As a result of experimenting with load and body pressure distribution and desirability to validate the device's bearing pressure, the acupressure rod held up to 150kg, the body pressure ratio was measured lower than the body pressure ratio of the comparison item in section 0%

• Journal of Mushroom
• /
• v.18 no.4
• /
• pp.380-386
• /
• 2020

The study was conducted for the safety evaluation of 320 pesticide residues in 768 Lentinula edodes fruit body samples and 143 L. edodes media samples, which are distributed nationwide in South Korea. The monitoring method was the second of the multi-residue methods in the Korean Food Code. GC-ECD, GC-NPD, and GC-MSD were used as evaluation equipment for analysis. Single-analysis of the target pesticides was performed for mepiquat chloride. Through the analysis of collected L. edodes samples, pesticide residues were detected in total seven cases, including four L. edodes fruit body samples and three L. edodes media samples. The detected pesticide residues were carbendazim, diflubenzuron, fluopyram, and dinotefuran. In this study, carbendazim was detected in three L. edodes fruit body samples and one L. edodes media sample. The detected amount of carbendazim was 0.056, 0.17, 0.043, and 0.09 mg/kg, respectively. The amount of carbendazim in the collected L. edodes samples was detected below the MRLs (maximum residue level). The detected amounts of fluopyram and dinotefuran were 0.068 mg/kg and 0.06 mg/kg, respectively. Two pesticide residues were detected in the medium in one case. Mepiquat chloride was not detected in this study. These results suggested that residual pesticides were detected in a small number of collected L. edodes. However, the PLS for unregistered pesticides MRL was 0.01 ppm; therefore, we have to conduct research on preparing safety standards for mushrooms, including L. edodes.

• Journal of Life Science
• /
• v.32 no.6
• /
• pp.438-446
• /
• 2022

Some flowers have a high sensual appeal owing to their unique shape, color, smell, and taste and have been used as functional food and oriental medicine. Recently, edible dry flowers (EDFs) have attracted social attention as noble sources of functional teas. In this study, for the risk assessment of EDFs, pathogenic bacteria, heavy metals, and pesticide residues were monitored in 23 types of commercial EDF. No Enterobacteria spp. and Listeria spp. were found in all EDF products. However, common aerobic bacteria (3.24~3.85 Log CFU/g) were found in EDF, namely, Pueraria lobata, Chamaemelum nobile, Acacia decurrens, Rhododendron mucronulatum Turcz, Oenothera lamarckiana, Brassica napus, and Prunus serrulata. Staphylococcus aureus was found in 11 and Salmonella sp. was found in 8 of the 23 EDFs. Considering the cold extraction of EDF for tea and beverages, the regulation of pathogenic bacteria in EDFs is necessary. No heavy metals such as Pb, Cd, Co, Cr, Cu, Ni, and As were found in all EDFs, except the dry flower of Hemerocallis fulva, which contained Pb at 0.08 ppm. Different pesticides and fungicides were found in EDFs, but their concentrations were very low (0.01~0.08 ppm) and below the maximal residue level. Only the dry flower of Chrysanthemum morifolium had a high content of chlorpyrifos (0.215 ppm), which is long-lasting pesticide. Our results suggest that the establishment of EDF regulations for pesticide residue, culture separation between edible and garden flowers, and guidelines for preventing pathogenic microbial contamination are necessary.

• Journal of Life Science
• /
• v.32 no.7
• /
• pp.501-509
• /
• 2022

Pollen corrected from Quercus species is abundant in Korea and has been used to treat anemia and inflammation of the prostate gland. It is also used for hemostasis in oriental medicine. In this study, the ethanol extract of the acorn pollen and its subsequent organic solvent fractions using hexane, ethylacetate, butanol, and water residue were prepared, and their antioxidant, anti-thrombosis, and hemolysis activities were evaluated. The ethylacetate fraction of acorn pollen (EF-AP) showed the highest polyphenol content (225.0 mg/g) and strong antioxidant activity among the solvent fractions. The RC₅₀ of EF-AP against DPPH, ABTS, and nitrite radicals was 72.2, 27.7, and 62.6 ㎍/ml, respectively. The blood coagulation activities of the all-solvent fractions determined by thrombin time, prothrombin time, and activated partial thromboplastin time were negligible up to 5 mg/ml. Platelet aggregation inhibitory activities were observed in the EF-AP, butanol fraction, and water residue, whereas hexane fraction induced strong platelet aggregation. The EF-AP has no hemolysis activity against human RBC up to 1 mg/ml. UPLC/MS/MS analysis of the EF-AP revealed that rutin, isoquercitrin, and astragalin are major compounds for antioxidant and anti-thrombosis activities. Our results suggest that EF-AP could be developed as a noble antioxidant and anti-thrombosis agent.

• Journal of the Korean Applied Science and Technology
• /
• v.38 no.2
• /
• pp.368-377
• /
• 2021

The present study aimed to evaluate the effects of radiation mutant Perilla frutescens var. crispa and Atractylodes macrocephala Koidzumi complex extract(Perilla frutescens var. crispa complex extract) on the mediators related to degenerative arthritis in a monosodium iodoacetate-induced rat model of degenerative arthritis. Perilla frutescens var. crispa complex extract was administered orally at doses of 25, 50 or 100 mg/kg/day for 2 weeks before direct injection of monosodium iodoacetate (3 mg/50 µl of 0.9% saline) into the intra-articular space of the rats' right knees. The rats subsequently received the same doses of oral Perilla frutescens var. crispa complex extract for another 4 weeks. It was evaluated that the treatment effects based on serum bio-markers, and morphological and histopathological analysis of the knee joints. Compared with those in negative control rats, the Perilla frutescens var. crispa complex extract treatments significantly reduced the serum levels of inflammation, bone metabolism markers (i.e., TNF-α, MMP-3, COX-2, PGE₂, COMP, and Aggrecan). Otherwise, it was significantly increased the production of CTX-2 in cartilage absorption mediators. In addition, the Perilla frutescens var. crispa complex extract treatments effectively preserved the knee cartilage and synovial membrane. As a result, it indicates that the Perilla frutescens var. crispa complex extract improved degenerative arthritis symptoms. Thus, the Perilla frutescens var. crispa complex can be used in food material for the management of degenerative arthritis.