• Title/Summary/Keyword: Organism

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Optimization of Tannase Production by Aureobasidium pullulans DBS66

  • Banerjee, Debdulal;Pati, Bikas R.
    • Journal of Microbiology and Biotechnology
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    • v.17 no.6
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    • pp.1049-1053
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    • 2007
  • Tannase production by Aureobasidium pullulans DBS66 was optimized. The organism produced maximum tannase in the presence of 1% tannic acid after 36 h. Maximum gallic acid accumulation was observed within 36 h and tannic acid in the fermented broth was completely degraded after 42 h of growth. Glucose had a stimulatory effect on tannase synthesis at 0.1% (w/v) concentration. The organism showed maximum tannase production with $(NH_4)_2HPO_4$ as nitrogen source. Shaking speed of 120 rpm and 50-ml broth volume have been found to be suitable for maximum tannase production.

Variations in Species Composition, Biomass, and Density in Shrimp Trawl Bycatch Across Seasons and Tidal Phases in Southern Korean Waters: Developing a Fisheries Risk Management Approach

  • Oh, Chul-Woong;Kim, Sung-Tae;Na, Jong-Hun
    • Fisheries and Aquatic Sciences
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    • v.12 no.2
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    • pp.138-151
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    • 2009
  • We quantitatively investigated compositions of fish and invertebrate bycatch in Korean shrimp fisheries. We sampled shrimp trawl bycatch from 48 commercial trawls over 1 year. These samples contained 108 taxa from 50 families, with 60 fish taxa contributing 51.2% of the total biomass and 48 invertebrate taxa 48.8%. However, 86.32% of the total individual organism density comprised invertebrates, while individual fishes made up the remainder. Within the bycatch, two fish species varied in population size structure by season, suggesting recruitment is seasonal in these taxa. Overall general catch characteristics (total bycatch biomass and organism density) varied significantly by season and tide, and a significant interaction effect was observed (season$\times$tide) on total density, but not on total biomass. The data collected will be used in designing a program of long-term bycatch monitoring.

Caenorhabditis elegans as a Biological Model for Multilevel Biomarker Analysis in Environmental Toxicology and Risk Assessment

  • Choi, Jin-Hee
    • Toxicological Research
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    • v.24 no.4
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    • pp.235-243
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    • 2008
  • While in some instances, loss of diversity results from acute toxicity (e.g. major pollution incidents), in most cases it results from long-term sub-lethal effects that alter the relative competitive ability and fitness of certain organisms. In such cases the sub-lethal effects will cause a physiological response in the organism that ultimately leads to community level changes. Very sensitive tools are now available to study sub-lethal responses at the molecular level. However, relating such laboratory measurements to ecological effects represents a substantial challenge that can only be met by investigation at all scales (molecular, individual organism and community level) with an appropriate group of organisms. Among the various in vertebrates which can be used as model organisms in such a way, the soil nematode, Caenorhabditis elegans appear to be a promising biological model to diagnose environmental quality. This paper reviews the current status of multilevel biomarkers in environmental toxicology, and C. elegans as promising organisms for this approach.

A Study on the Treatment of Paper Mill Wastewater with the Addition of High Thermal Microbial Inoculants (고온성 종균제를 이용한 제지폐수 처리)

  • 이성호;임택준;조준형
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.34 no.2
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    • pp.54-60
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    • 2002
  • The Wastewater of toilet paper mill recycling recovered milk carton was used as a raw material for this study. According to the actual mill conditions, hydraulic retention time was adjusted to 12 hours and F/M (Food/Micro-organism) ratio was adjusted to 0.23. Temperature of aeration basin was varied from 2$0^{\circ}C$ to 5$0^{\circ}C$. The change of Micro-organisms and removal efficiency of pollutant were investigated at the varied temperature of basin. Aeration basin using high thermal microbial inoculants showed more removal efficiency of SS, COD than aeration basin using conventional microbial inoculants at high temperature. Floc consolidation of aeration basin using high thermal microbial inoculants added sludge was better than that of sludge from aeration basin using conventional microbial inoculants.

Microbial Genome Analysis and Application to Clinical Bateriology (미생물의 유전자(Genome) 해석과 임상세균학에 이용)

  • Kim, Sung-Kwang
    • Journal of Yeungnam Medical Science
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    • v.19 no.1
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    • pp.1-10
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    • 2002
  • With the establishment of rapid sequence analysis of 16S rRNA and the recognition of its potential to determine the phylogenetic position of any prokaryotic organism, the role of 16S rRNA similarities in the present species definition in bacteriology need to be clarified. Comparative studies clearly reveal the limitations of the sequence analysis of this conserved gene and gene product in the determination of relationship at the pathogenic strain level for which DNA-DNA reassociation experiments still constitute the superior method. Since today the primary structure of 16S rRNA is easier to determine than hybridization between DNA strands, the strength of the sequence analysis is to recognize the level at which DNA pairing studies need to be performed, which certainly applies to similarities of 97% and higher.

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REGISTRATION OF MICROSCOPIC SECTION IMAGES BASED ON A RADIAL DISTORTION MODEL

  • Lee, Hoo-Sung;Yun, Il-Dong;Kim, Dong-Sik
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2009.01a
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    • pp.303-306
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    • 2009
  • Registration of microscopic section images from an organism is of importance in analyzing and understanding the function of an organism. Microscopes usually suffer from the radial distortion due to the spherical aberration. In this paper, a correction scheme for the intra-section registration is proposed. The correction scheme uses two corresponding feature points under the radial distortion model. Proposing several variations of the proposed scheme, we extensively conducted experiments for real microscopic images. Iterative versions of the correction from multiple feature points provide good performance for the registration of the optical and scanning electron microscopic images.

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The Production of Biopolymer by Zoogloea ramigera (Zoogloea ramigera에 의한 생물고분자 생산에 관한 연구)

  • 안대희;권해수정윤철
    • KSBB Journal
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    • v.7 no.3
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    • pp.166-171
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    • 1992
  • Zoogloea ramigera 115 was cultured for biopolymer production and its bioflocculant usages. Cultural conditions of the organism were examined with regard to high production of the microbial polysaccharide. The most suitable medium was found to contain glucose as a carbon source, $NaNO_3$ as a nitrogen source, and yeast extract as an organic nutrient. The initial pH of 6.0 proved to optimal. The biopolymer was extracted effectively using ultrasonication and high speed centrifugation, followed by propanol addition. Jar test results indicate that the polysaccharide produced by the organism is an effective flocculant.

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Actinomycosis of the Foot - A Case report - (족부에 발생한 방선균증 - 1예 보고 -)

  • Kim, Chong-Kwan;Kim, Jeong-Whan;Hong, Jeong-Gee;Kwon, Oh-Joon
    • Journal of Korean Foot and Ankle Society
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    • v.4 no.1
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    • pp.30-34
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    • 2000
  • The Actinomyces organism is inherent in human as normal flora found in the mouth. Outside of the mouth, this organism can cause actinomycosis. Involvement of the extremity is very rare and atypical. The authors have experienced a case of actinomycosis of the foot and report with the literature review. Histopathologic finding shows multiple sulfur granules. In histochemical stains, periodic acid-Schiff, Gomori methionine silver, and gram stain were positive, and acid fast bacilli stain was negative. The patient was treated with surgical excision and antibiotic administration with Ampicillin for 6 months.

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A Comparative Study on the Morphological Characteristics of PAO and dPAO Granule (PAO와 dPAO 입상슬러지의 형태학적 특성에 대한 비교 고찰)

  • Yun, Geumhee;Yun, Zuwhan
    • Journal of Korean Society on Water Environment
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    • v.33 no.3
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    • pp.302-310
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    • 2017
  • The morphological characteristics of granules developing in anaerobic-anoxic (An-Ax) and anaerobic-aerobic (An-Ox) sequencing batch reactors (SBRs) were examined. The granules developed in the both SBRs after 200 days of laboratory operation. The average diameters of the granules were $2.2{\pm}1.7mm$ in the An-Ax SBR and $0.4{\pm}0.3mm$ in the An-Ox SBR. To determine the possible factors affecting morphology of granules a comparative analysis of various operating conditions from reference data indicated that the availability and type of electron acceptors is a key factor determining the granulation process and granular morphology.

Trend and Technology of Gene and Genome Research (유전자 및 유전체 연구 기술과 동향)

  • 이진성;김기환;서동상;강석우;황재삼
    • Journal of Sericultural and Entomological Science
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    • v.42 no.2
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    • pp.126-141
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    • 2000
  • A major step towards understanding of the genetic basis of an organism is the complete sequence determination of all genes in target genome. The nucleotide sequence encoded in the genome contains the information that specifies the amino acid sequence of every protein and functional RNA molecule. In principle, it will be possible to identify every protein resposible for the structure and function of the body of the target organism. The pattern of expression in different cell types will specify where and when each protein is used. The amino acid sequence of the proteins encoded by each gene will be derived from the conceptional translation of the nucleotide sequence. Comparison of these sequences with those of known proteins, whose sequences are sorted in database, will suggest an approximate function for many proteins. This mini review describes the development of new sequencing methods and the optimization of sequencing strategies for whole genome, various cDNA and genomic analysis.

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