• 한국작물학회지
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• 제36권4호
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• pp.332-339
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• 1991

본 시험은 1968-1990년까지 23년간을 동일시험구에서 무비구를 비롯하여 무질소구, 무인산구, 무가리구, 3요소구, 3요소+유기물구(퇴비 또는 생고), 3요소十석회구를 설치하고, 1990년수도품종 ‘진흥’을 공시하여 그 수량생산성과 미질의 외관 및 이화학적특성을 조사하므로써 장기간에 걸친 시비법이 수량과 양질미생산에 미치는 영향을 검토한 바 그 결과를 요약하면 다음과 같다 1 수량생산성은 3요소구에 비해 3요소+유기물구에서 3-7%의 증수효과가 있었으며, 1 증수요인은 수수와 1수당입수의 증가였다. 2. 무가리구의 수량은 85%로 등숙률의 저하가 감수요인이며, 무인산구는 74%로 수수의 감소가 감수요인이며, 무질소구와 무비구는 59-33%로 수수와 1수립수의 현저한 감소가 감소요인이었다. 3. 미질의 외관적 특성은 무비, 무질소, 무인산구에서 수량은 크게 낮은 반면에 완전미율이 높고 동할미, 심복백미율이 현저히 적으며 3미립의 투명도도 높아 양질의 외관을 보였다. 이에 대해 고수량을 보인 3요소＋유기물구(퇴비, 생고)에서 완전미율이 낮고 동할미, 심복백미율이 높고 투명도가 낮아 외관적 미질이 저하하는 경향이었다. 4 미질검정으로 이용되고 있는 물리적 특성인 알칼리붕괴성과 gel consistency는 처리간 차이를 인정할 수 없었다. 5. 식미의 량부로 평가하고 있는 미립의 화학적 특성은 처리간 차이를 인정할 수 있었다. 즉 단백질함량은 3요소＋유기물구에서 높고 무비구에서 낮았으며 Mg, Mg/K, Mg/KㆍN, Mg/KㆍN\ulcornerAmylose는 무비, 무질소, 무가리구에서 높고, 3요소+유기물. 3요소+석회구에서 낮았다 6. 이상과 같은 결과로 미루어 본 실험에 시용된 절대질소량은 많지 않음에도 불구하고 3요소＋유기물구는 유기물 분해에 따른 질소의 추비적 효과가 나타나서 무비, 무질소구에 비해 동할미, 심복백미 등 미립의 외관적 특성을 저하시키고, 단백질함량은 높이고 Mg/K\ulcornerNㆍAmylose를 낮추어 식미를 저하시키는 것으로 해석된다 7. 취급특성으로서의 흡수율, 취급액요드정색도, 취급용액출고형물은 처리간 명백한 경향을 발견할 수 없었다.

• Applied Biological Chemistry
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• 제33권2호
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• pp.133-137
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• 1990

담수토양중(湛水土壤中)에 있어서 IBP의 분해(分解)에 미치는 각종(各種) 토양환경조건(土壤環境條件)의 영향(影響)에 대하여 연구검토(硏究檢討)하였다. IBP는 비담수조건(非湛水條件)에서 보다 담수조건(湛水條件)에서 느리게 분해(分解)되었다. 분해속도(分解速度)는 토양종류(土壤種類)에 따라 크게 변화(變化)되었으며, 유기물함양(有機物含量)이 높은 토양(土壤)에서보다 낮은 토양(土壤)에서 빨랐다. IBP는 저온(低溫)에서보다 고온(高溫)의 토양(土壤)에서, 고농도(高濃度)에서보다 저농도(低濃度) 첨가(添加)에서 빨리 분해(分解)되었다. 토양중(土壤中) 분해속도(分解速度)는 볏짚첨가에 의하여 현저히 저하(低下)되었으나, 복합비료(複合肥料)나 타농약(他農藥) fenitrothion과 butachlor의 첨가(添加)에 의하여는 영향(影響)을 받지 않았다. 토양중(土壤中) IBP는 호기적(好氣的) 미생물(微生物)에 의하여 분해(分解)되는 것으로 추정(推定)되었다.

• KSBB Journal
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• 제18권6호
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• pp.466-472
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• 2003

본 연구에서는 가축분뇨의 비료화를 위한 분뇨의 악취와 암모니아성 질소를 제거시킬 수 있는 기능성 발효촉진 미생물의 선별을 목표로 쌀겨 자연발효 추출물로부터 총 61개의 균주를 순수 분리하였고, 순수 분리된 균주의 단백질, 지방, 탄수화물 등의 유기물 분해 효소활성 (amylase, protease, cellulase 및 lipase), 암모니아 가스의 탈취성능을 조사하여 최종적으로 NA 2, 12, 15의 3종의 균주를 선별하였다. 최종 선별된 균주에 대해서 동정실험을 수행한 결과, NA 2는 Bacillus acidocaldarius로 동정되었고, NA 15는 Planoroccus sp.로 부분동정되었다. 이들 분리된 미생물을 가축분뇨처리제로 개발하기 위한 최적의 혼합 배양 조건을 구하기 위해 반응표면계획법으로 배지 조성과 pH와 같은 조작 변수들의 영향과 상호작용 등을 포함한 데이터를 분석한 결과, 최적 배양 조건은 beef extract 4.59g/L, Peptone 8.73g/L, PH 6.3으로 결정되었다. 분리된 미생물들은 발효촉진 및 암모니아 탈취 성능이 우수하면서도 중$.$고온성 미생물들로서 가축분뇨처리에 활용가능성이 높은 것으로 판단되었다.

• Journal of Microbiology and Biotechnology
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• 제20권5호
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• pp.925-934
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• 2010

Three typical biological solid wastes, namely, animal manure, garbage, and sewage sludge, were compared with regard to the composting process and the changes in microbial community structure. The effects of different bulking agents such as rice straw, vermiculite, sawdust, and waste paper were compared in manure compost. The differences in the microbial community were characterized by the quinone profile method. The highest mass reduction was found in garbage composting (56.8%), compared with manure and sludge (25% and 20.2%, respectively). A quinone content of $305.2\;{\mu}mol/kg$ was observed in the late stage of garbage composting, although the diversity index of the quinone profile was 9.7, lower than that in manure composting. The predominant quinone species was found to be MK-7, which corresponds to Gram-positive bacteria with a low G+C content, such as Bacillus. The predominance of MK-7 was especially found in the garbage and sludge composting process, and the increase in quinones with partially saturated long side-chains was shown in the late composting process of manure, which corresponded to the proliferation of Actinobacteria. The effects of different bulking agents on the composting process was much smaller than the effects of different raw materials. High organic matter content in the raw materials resulted in a higher microbial biomass and activity, which was connected to the high mass reduction rate.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1288-1299
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• 2017

Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw. A total of 25 operational taxonomic units were classified based on 16S rRNA gene clone libraries, and 14 operational taxonomic units were classified based on hzo gene clone libraries. The phylogenetic tree analysis of the 16S rRNA gene and deduced HZO protein sequences from the corresponding encoding genes indicated that the majority of the obtained clones were related to the known anammox bacteria Candidatus "Brocadia," Candidatus "Kuenenia," and Candidatus "Scalindua." The abundances of anammox bacteria were determined by quantitative PCR, and between $2.13{\times}10^5$ and $1.15{\times}10^6$ 16S rRNA gene copies per gram of compost were found. This study provides the first demonstration of the existence of anammox bacteria with limited diversity in cow manure composting.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1688-1696
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• 2020

Soil physical and chemical characteristics, soil potential denitrification rates (PDR), community composition and nirK-, nirS- and nosZ-encoding denitrifiers were studied by using MiSeq sequencing, quantitative polymerase chain reaction (qPCR), and terminal restriction fragment polymorphism (T-RFLP) technologies base on short-term (5-year) tillage field experiment. The experiment included four tillage treatments: conventional tillage with crop residue incorporation (CT), rotary tillage with crop residue incorporation (RT), no-tillage with crop residue retention (NT), and rotary tillage with crop residue removed as control (RTO). The results indicated that soil organic carbon, total nitrogen and NH₄⁺-N contents were increased with CT, RT and NT treatments. Compared with RTO treatment, the copies number of nirK, nirS and nosZ in paddy soil with CT, RT and NT treatments were significantly increased. The principal coordinate analysis indicated that tillage management and crop residue returning management were the most and the second important factors for the change of denitrifying bacteria community, respectively. Meanwhile, this study indicated that activity and community composition of denitrifiers with CT, RT and NT treatments were increased, compared with RTO treatment. This result showed that nirK, nirS and nosZ-type denitrifiers communities in crop residue applied soil had higher species diversity compared with crop residue removed soil, and denitrifying bacteria community composition were dominated by Gammaproteobacteria, Deltaproteobacteria, and Betaproteobacteria. Therefore, it is a beneficial practice to increase soil PDR level, abundance and community composition of nitrogen-functional soil microorganism by combined application of tillage with crop residue management.

• 대한영양사협회학술지
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• 제24권1호
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• pp.62-74
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• 2018

The purpose of this study was to investigate dietitians or nutrition teachers' perception of sugar reduction and usage of sugars in school meal service. A survey was conducted for dietitians or nutrition teachers at elementary, middle, and high schools in Gyeonggi and Incheon. Survey data were analyzed according to the experience period and school types of dietitians or nutrition teachers. The numbers of subjects according to work experience period were 134 (44.2%) for <5 years, 68 (22.4%) for ${\geq}5$ and <10 years, and 101 (33.3%) for ${\geq}10years$. Among the subjects, 168 (55.4%) were nutrition teachers at kindergarten/elementary schools, and 135 (44.6%) were at middle/high/special schools. Perceptions of sugar reduction programs were higher in kindergarten/elementary schools than in middle/high/special schools. The perception of added sugar use increased with more work experience, and interest in sugar reduction was higher in kindergarten/elementary schools than in middle/high/special schools. Practice for added sugar reduction increased with more work experience, and the most common method for reducing sugar was use of natural sugars. The main reason for not reducing added sugars in school meals was due to preference. Nutritional education for students increased with more work experience and was higher in kindergarten/elementary schools than in middle/high/special schools. The major nutritional education methods were using school homepage (37.4%), sending family letters (37.4%), and using principle's discretion time (18.4%). Organic sugar and rice taffy were used more at kindergarten/elementary schools, and white sugar, brown sugar, and white syrup were used more at middle/high/special schools. The most common menu using added sugars was stir-fried dishes (stir-fried pork, chicken, and vegetables).

• Journal of Plant Biology
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• 제12권1호
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• pp.7-14
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• 1969

Korea has a lots of margin for security of farm land from her coastal region. The area of saline soil may be reached about 10% of present farm land if the reclamation works are finished. This paper was conducted as a part of studying the possibilities of desalination of saline soil through the experiment of some halophytes. The halophytes in this works were Salicornia herbacea L., Suaeda glauca Bunge, chenopodium acuminatum Willd, and Scirpus triquerter L. Of the above halophytes, Salicornia was proved the most effective plant for desalination of saline soil referring to the following results; 1) The seasonal uptake of chloride by Salicornia was the highest of all. However, the general tendencies of all plants showed a decrease on August. 2) Salinity of soil showed the lowest value on the site where Salicornia was grwon densely. Comparing the other sites grouped by age of saline soil with the above site, the salinity of rice-paddy (10 years after reclamation) is similar to those of the site wehre Salicornia were as well as the 50 cm below the surface soil. 3) The maximum water holding capacity of surface soil appeared in the site of Salicornia, but in 50 cm below the surface, the maximum water holding capacity are almost on equat terms having no connection with the age of saline soil. Soil pH, other chemical compositions such as organic matter, magnesium, potassium, phosphorous, and nitrate were determined to elucidate the relationship between the changes of soil properties and chemical uptakes by certain halophytes. It is assumed that the above chemical compositions are frequently affected by the factors such as coastal circulation of salts, exchangeable base, microbial growth, climatic conditions, and irrigation of water.

• 한국토양비료학회지
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• 제49권5호
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• pp.434-439
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• 2016

Anthropogenic soil in cropland is formed in the process of subsoil reversal and the refill of soil into cropland. However, there was little information on the chemical properties within soil profiles in anthropogenic soil under rice paddy near the river. In this study, we investigated the chemical properties within soil profiles in the anthropogenic soil located at 4 sites in Gumi, Kimhae, Chungju, and Euiseong to compare with the natural paddy soil near the river. Among particle sizes, the sand content decreased under soil profiles but the silt and clay contents increased compared to the natural paddy soil in soil profiles. Organic matter content in topsoil of anthropogenic soil was lower than in that of natural soil, which was shown the contrary tendency within soil profiles. Also, the soil pH, available $P_2O_5$, and exchangeable cations were higher in anthropogenic soil compared to natural paddy soil at topsoil, which was maintained these tendency into soil depth. Nutrients may be equally distributed in anthropogenic soil during the process of refill in paddy soil near the river. This results indicated that anthropogenic soil would contribute to carbon sequestration, the mitigation of compaction, and reduction of fertilizer application in paddy soil. Therefore, characteristics of anthropogenic soil can be used for the soil management in cropland.

• 한국토양비료학회지
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• 제46권2호
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• pp.73-80
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• 2013

Due to the fact that possible risk associated with soil-crop-food chain transfer, metal contamination in croplands has become a major topic of wide concern. Accumulation of toxic metals in edible parts of crops grown in contaminated soils has been reported from number of crops including rice, soybean, wheat, maize, and vegetables. Therefore, in order to ensure food safety, measures are needed to be taken in mitigating metal pollution and subsequent uptake by crop plants. Present paper critically reviewed some of the cost effective remediation techniques used in minimizing metal uptake by crops grown in contaminated soils. Liming with different materials such as limestone ($CaCO_3$), burnt lime (CaO), slaked lime [$Ca(OH)_2$], dolomite [$CaMg(CO_3)_2$], and slag ($CaSiO_3$) has been widely used because they could elevate soil pH rendering metals less-bioavailable for plant uptake. Zn fertilization, use of organic amendments, crop rotation and water management are among the other techniques successfully employed in reducing metal uptake by crop plants. However, irrespectively the mitigating measure used, heterogeneous accumulation of metals in different crop species is often reported. The inconsistency might be attributed to the genetic makeup of the crops for selective uptake, their morphological characteristics, position of edible parts on the plants in respect of their distance from roots, crop management practices, the season and to the soil characteristics. However, a sound conclusion in this regard can only be made when more scientific evidence is available on case-specific researches, in particular from long-term field trials which included risks and benefits analysis also for various remediation practices.