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Bipolar Heterothallism, a Principal Mating System of Cordyceps militaris In Vitro

  • Shrestha Bhushan;Kim Ho Kyung;Sung Gi Ho;Spatafora Joseph W.;Sung Jae Mo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.440-446
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    • 2004
  • Interest in in vitro study of entomopathogenic fungi, including Cordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. Among Cordyceps species, in vitro stromata of C militaris has been successfully produced and cultivated for industrial purposes. However, genetic study on in vitro stromata formation of C militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation of C militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, mono­ascospore cultures without combination produced either no stromata or only abnormal non­perithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains of C militaris. These observations indicated that C militaris behaves as a bipolar het­erothallic fungus and requires two mating compatible strains in order to produce regular club­shaped perithecial stromata, a fundamental requirement for its industrial cultivation.

The Degradation of Wood and Pulp by Wood-degrading Fungi

  • Jung Hyunchae;Geng Xinglian;Li Kai Chang
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.5 s.108
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    • pp.69-77
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    • 2004
  • Degradations of pine, yellow poplar and sweet gum by two fungi, Pycnoporus cinnabarinus and Trichophyton rubrum LSK-27 were investigated. P. cinabarinus degraded pine block samples much faster than T rub rum LSK-27, whereas P. cinnabarinus and T rubrum LSK-27 degraded yellow poplar and sweet gum at almost the same rate. In an effort to get a better understanding of how fungi degrade lignin in wood, contents of various functional groups were analyzed. After three-months of degradation of pine flour by these fungi, the following changes were observed: an increase in condensed phenolic OH group and carboxylic acid group content, a decrease in the guaiacyl phenolic OH content, and little change of aliphatic OH group content. Further studies in the degradation of pine flour by P. cinnabarinus indicated that the increase in condensed phenolic OH group content and the decrease in guaiacyl phenolic OH group content occurred in the first month of the degradation. The changes of functional group contents in the degradation of unbleached softwood kraft pulp by P. cinnabarinus had the same trends as those in the degradation of pine flour. That is, structural alteration of lignin due to the kraft pulping process had little effect on how P. cinnabarinus degraded lignin.

Hydroacoustic Records and Numerical Models of the Source Mechanisms from the First Historical Eruption of Anatahan Volcano, Mariana Islands

  • Park M.;Dziak R.P.;Matsumoto H.
    • 한국지구물리탐사학회:학술대회논문집
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    • 2004.06a
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    • pp.232-237
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    • 2004
  • Anatahan Volcano in the Commonwealth of the Northern Mariana Islands (CNMI) erupted for the first time in recorded history on 10 May 2003. The underwater acoustic records of earthquakes, explosions, and tremor produced during the eruption were recorded on a sound-channel hydrophone deployed in February 2003. Acoustic propagation models show the seismic to acoustic conversion at Anatahan is particularly efficient, aided by the slope of the seamount toward the hydrophone. The hydrophone records confirm the onset of earthquake activity at 01:53Z on 10 May, as well as the onset (at ${\~}$06:20Z) of continuous, low-frequency (5-40 Hz) acoustic energy that is likely volcanic tremor related to magma intrusion. The hydrophone recorded a total of 458 earthquakes associated with the eruption. To predict the character of acoustic signals generated from Anatahan, we developed a moment-tensor representation of a volcano-seismic source that is governed by the geometry of the source and the physical properties of the magma. A buried magmatic pipe model was adopted, and numerically modeling source parameters such as the pipe radius and magma viscosity enable us to grasp the inward nature of Anatahan Volcano.

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Flora of drift plastics: a new red algal genus, Tsunamia transpacifica(Stylonematophyceae) from Japanese tsunami debris in the northeast Pacific Ocean

  • West, John A.;Hansen, Gayle I.;Hanyuda, Takeaki;Zuccarello, Giuseppe C.
    • ALGAE
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    • v.31 no.4
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    • pp.289-301
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    • 2016
  • Floating debris provides substrates for dispersal of organisms by ocean currents, including algae that thrive on plastics. The 2011 earthquake and tsunami in Tohuku, Japan resulted in large amounts of debris carried by the North Pacific Current to North America from 2012 to 2016. In 2015-2016, the plastics in the debris bore a complex biota including pink algal crusts. One sample (JAW4874) was isolated into culture and a three-gene phylogeny (psbA, rbcL, and SSU) indicated it was an unknown member of the red algal class Stylonematophyceae. It is a small pulvinate crust of radiating, branched, uniseriate filaments with cells containing a single centrally suspended nucleus and a single purple to pink, multi-lobed, parietal plastid lacking a pyrenoid. Cells can be released as spores that attach and germinate to form straight filaments by transverse apical cell divisions, and subsequent longitudinal and oblique intercalary divisions produce masses of lateral branches. This alga is named Tsunamia transpacifica gen. nov. et sp. nov. Sequencing of additional samples of red algal crusts on plastics revealed another undescribed Stylonematophycean species, suggesting that these algae may be frequent on drift oceanic plastics.

Employers' Perceptions of Intimate Partner Violence among a Diverse Workforce

  • Samuel, Laura J.;Tudor, Carrie;Weinstein, Marc;Moss, Helen;Glass, Nancy
    • Safety and Health at Work
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    • v.2 no.3
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    • pp.250-259
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    • 2011
  • Objectives: Intimate partner violence (IPV) is a significant global public health concern, affecting 5.3 million US individuals annually. An estimated 1 in 3 women globally are abused by an intimate partner in their lifetime, and the effects carry over into the workplace. This article examines employers' perceptions of IPV in the workplace, targeting supervisors of Latina employees. Methods: Fourteen employers and supervisors of small service-sector companies in Oregon were interviewed using semi-structured interviews. Interpretive description was used to identify themes. These qualitative interviews preceded and helped to formulate a larger workplace intervention study. Results: The following themes were found and are detailed: (1) factors associated with recognizing IPV in the workplace, (2) effects of IPV on the work environment and (3) supervisors' responses to IPV-active vs. passive involvement. Also, supervisors' suggestions for addressing IPV in the workplace are summarized. Conclusion: These findings demonstrate the need for more IPV-related resources in the workplace to be available to supervisors as well as survivors and their coworkers. The needs of supervisors and workplaces vary by site, demonstrating the need for tailored interventions, and culturally appropriate workplace interventions are needed for Latinas and other racially and ethnically diverse populations.