• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.531-541
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• 2018

Purpose : To evaluate the acute cytotoxic effect of polyhexamethylene biguanide (PHMB), epigallocatechin gallate (EGCG) and PHMB/EGCG mixture on the cultured human corneal epithelial cell (HCEpiC). Methods : HCEpiCs were cultured in the media of HCEpiC containing 0.00001~0.005% PHMB, 0.001~5% EGCG and 0.00005% PHMB/0.05% EGCG mixture respectively for 30, 60, 120 and 240 min. Cultured HCEpiCs were fixed and stained with Draq5 and cell viability and apoptosis were evaluated using confocal microscope and ImageXpress $Ultra^{TM}$. Results : Cultured HCEpiC did not show cytotoxic effect at below 0.00005% PHMB and below 0.05% EGCG concentration. In the media containing 0.00005% PHMB/0.05% EGCG, acute cytototoxic effect was not found, whereas damaged HCEpiCS were increased and survival cells were decreased in the media incubated for 240 min. Conclusion : The mixture of 0.00005% PHMB/0.05% EGCG showed non acute cytotoxic effect on the cultured HCEpiCs, however it is needed to investigate its chronic cytotoxic effect.

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.431-442
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• 2018

Purpose : The purpose of this study was to investigate subjective symptoms according to the wearing, purchase and fitting status of soft contact lens wearers, and consumer perception of base curve. Methods : A survey was conducted for those who visited optical shops and lens shops in Seoul from June to August 2018. A total of 98 answer sheets they submitted were used for the analysis. The chi-square test and Fisher's exact test were used to compare subjective symptoms (dryness, glare, uncomfortable fitting, and decreased vision) according to whether fitting condition is screened (case history and push-up test, ect relevant to wearing sensation), and the odds ratio (OR) was obtained by the logistic regression analysis. Results : When the contact lenses were purchased, the rate of which the visual acuity test was performed was 86.6% at optical shop and 64.7% at lens shop. When purchasing contact lens and the contact lens was not tested for fitting, they more experienced dryness (OR 4.41, 95% CI 1.25-15.62) and uncomfortable fitting (OR 2.68, 95% CI 1.08-6.64) than testing for fitting. In addition, it was investigated that 87.8% of contact lens wearers did not know about the term base curve, 92.9% did not listen to an explanation related to the base curve when purchasing contact lenses, and 96.9% did not experience with base curve test. Conclusion : It would be thought that satisfaction of the contact lens fitting of existing consumers should be improved by changing a proper base curve by confirming the fitting condition when prescribing soft contact lens.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.1-7
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• 2018

In a microcolumn, a miniaturized electrostatic deflector is often adopted to scan an electron beam. Usually, a double octupole deflector is used because it can avoid excessive spherical aberrations by controlling the electron beam path close to the optical axis of the objective lens and has a wide scan field. Studies on microcolumns have been performed to improve the low throughput of an electron column through multiple column applications. On the other hand, as the number of microcolumns increases, the number of wires connected to the components of the microcolumn increases. This will result in practical problems during the process of connecting the wires to electronic controllers outside of the vacuum chamber. To reduce this problem, modified quadrupole and octupole deflectors were examined through simulation analysis by selecting an ultraminiaturized microcolumn with the Einzel lens eliminated. The modified deflectors were designed changing the size of each electrode of the conventional Si octupole deflector. The variations of the scan field and electric field strength were studied by changing the size of active electrodes to which the deflection voltage was to be applied. The scan field increased linearly with increasing deflection voltage. The scan field of the quadrupole deflector and the electric field strength at the center were calculated to be approximately 1.3 ~ 2.0 times larger than those of the octupole deflector depending on the electrode size.

• The Korean Journal of Emergency Medical Services
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• v.22 no.3
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• pp.7-34
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• 2018

Purpose: The purposes of this research were to study the validity of the National Examination based on smart based test(SBT) by 7 types of healthcare occupation. Methods: We conducted a questionnaires survey among 1,219 people including 210 professors for all 7 types of healthcare occupation and 573 clinical specialists ; 436 students from random sampling participated and the reliability of the questionnaire was tested using Cronbach's alpha. Results: Professors from 7 types of healthcare occupation were investigated for their opinions on the introduction of the SBT system ; 65.9% of the professors answered positively on the introduction of the SBT National Examination System, while 40.1% of the clinical experts answered likewise. In particular, professors and the clinical experts recognized altogether that the multimedia questionnaire, being effective for the evaluation of clinical and practical competence of the subjects, enabling the evaluation of various areas and strengthening the school education in clinical practices, the 42.0% of the students from 7 departments answered that the future direction of the national test is in the SBT test system with significant difference (p=.000) found in students majoring in order of Emergency Medical Services, Radiology and Physical therapy, compared to students from other majors, showing the positive response to the SBT system. Conclusion: The results of this study comprehensively evaluated the positive recognition of development of multimedia items and SBT system in national examination of 7 types of healthcare occupation in professors, field specialists and students.

• The Korean Journal of Vision Science
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• v.20 no.4
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• pp.589-596
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• 2018

Purpose : We compared intraocular pressure (IOP) values measured by two types of tonometers in condition of normotensive and glaucomatous rat model. We tried to determine which of tonometer can more easily and accurately measure the IOP of animal model. Methods : Glaucomatous eyes were induced by intracameral injections of hyaluronic acid in right eyes of six-week-old male Spargue-Dawley (SD) rats. Normotensive contralateral eyes were left eyes of the SD rats. IOP was measured using a rebound tonometer (Tonolab) and a immersive tonometer ($Tonopen^{(R)}$ XL) about 3:00 pm. Results : The mean IOP values of normotensive control eyes were $10.80{\pm}1.03mmHg$ by Tonopen, and $15.10{\pm}0.73mmHg$ by Tonolab. They were statistically insignificant (p = .1). The mean IOP values of glaucomatous experimental eyes were $30.20{\pm}2.67mmHg$ by Tonopen, and $37.90{\pm}2.73mmHg$ by Tonolab. They were statistically insignificant (p = .95). High IOP values of glaucomatous eyes by two types of tonometers had strong positive correlation each other (r = .904, p < .01). Conclusion : This is the first study to compare IOP values using two types of tonometers between normotensive and glaucomatous model made by intracameral injection of hyaluronic acid. Tonopen should be used carefully when the IOP is within normal range, and both Tonopen and Tonolab can be used reliably when the IOP is high.

• Journal of Korean Clinical Health Science
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• v.9 no.1
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• pp.1478-1485
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• 2021

Purpose. To analysis of astigmatism in Korean adults from 20 to 29 years old. Methods. From January 2019 to February 2020, one hundred two subjects were performed in refraction test using the Auto-Refractometry. Results. In the type of astigmatism based on refractive error, the male's right eye was 4.26% simple myopic astigmatism and 95.74% complex myopic astigmatism, and the left eye was 2.22% simple myopic astigmatism and 97.78% complex myopic astigmatism. However, in the female's right eye, simple myopic astigmatism was 2.27%, complex myopic astigmatism was 97.73%, and left eye was simple myopic astigmatism was 2.17%, complex myopic astigmatism was 97.83%. The types of astigmatism in men and women similar to other. The mean refractive astigmatism values were -0.922±-0.798 diopter and -0.877±-0.707 diopter for male right and left eye, and -0.765±-0.599 diopter and -0.853±-0.586 diopter for female. On the other hand, the refraction values of the right and left eyes of men and women have statistical significance(p=0.000). The mean power comparative between refractive astigmatism and corneal astigmatism power were -0.186±-0.745 diopter, -1.091±-0.589 diopter in male and -1.140±-0.593 diopter, -1.140±-0.534 diopter in female. Therefore, the mean power values of the refractive astigmatism and corneal astigmatism of men and women were statistical significance(p=0.000). At the 0.75 diopter or more, which can have clinical symptoms of astigmatism power, males have 60.86% and 72.09% of the right and left eyes, and females have 47,64% and 60.10% of the right and left eyes. At 0.75 diopter or more, which can have clinical symptoms of corneal astigmatism power, males have 68.75% and 74% of the right and left eyes, and females have 86.50% and 82.35% of the right and left eyes. In the type of astigmatism axis, both men and women had a high frequency of the rule astigmatism. However, men had a high frequency of against-the-rule astigmatism and women showed a high frequency of oblique astigmatism. Conclusions. In this study, the complex myopic astigmatism and with-the rule astigmatism was the most common of all subjects. However, there was a statistical significance between right and left eyes of all subjects. The corneal astigmatism was higher refractive values in both males and females than refractive astigmatism. This study suggests that the analysis of the astigmatism can provide the useful diagnosis information for the correction of visual acuity.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.3
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• pp.224-238
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• 2022

The environment and scope of work of optometric personnel in ophthalmology are unknown. We aimed to investigate these topics using an online survey. Based on the results obtained, we also make policy proposals from the perspective of clinical laboratory technologists to the University-Association-Academy. The age distribution of the study subjects was as follows; 20s (40.7%), 30s (28.4%), 40s (23.5%), and 50s (7.4%). Women constituted 56.0% of the cohort, and careers were evenly distributed from <1 to ≥15 years. We found optometric personnel had high levels of work-related stress, frequent occupational diseases, and that their duties were wide in scope. More manpower is needed to reduce work stress and improve work satisfaction. Clinical laboratory technologists held more senior positions at 48% compared to others and the occupational distribution was closely related to the senior occupation (P<0.001). Clinical laboratory technologists were responsible for about 30 types of eye examinations, and the most frequently performed examination was the refraction test; anomaloscopic examinations took the most time (27 minutes). Taken together, clinical laboratory technologists perform various eye exams. Given the importance of clinical laboratory technologists in optometry, a multifaceted effort is required at the University-Association-Academy.

• Journal of Korean Ophthalmic Optics Society
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• v.19 no.2
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• pp.163-169
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• 2014

Purpose: This study is for compared the change of corneal refractive power before and after wearing of rigid gas permeable contact lense with diagnostic method which is 1 D flatter than alignment fitting on right eye and alignment fitting on left eye for 2 months and investigate the preference. Methods: Twenty middle school and high school students (40 eyes) who had never worn a contact lense before for no corneal topographical change, no ocular disease, no experience of ophthalmic surgery and have normal tear amount were selected for this study and corneal refractive power were examined before wearing rigid gas permeable contact lense and adaptation status and corneal examination were performed after 10 days of wearing and after cheking up the continuation of wearing, all candidate wear contact lens 8 hours per day for 2 month and corneal refractive power were compared. Results: After 2 months of wearing with 1 D flatter than the alignment fitting on right eyes, there was significant difference in the central corneal refractive power was $43.84{\pm}1.33D$, flat K power was $43.05{\pm}1.29D$, and steep K power was $44.61{\pm}1.42D$ decreased than before wearing (p<0.001, 0.001, 0.047). The e-value of the principal meridians also shows statistically significant difference (p=0.037, 0.015). After 2 months of wearing with alignment fitting on left eyes, the central corneal refractive power was $44.40{\pm}1.26D$, flat K power was $43.57{\pm}1.23D$. and flat K e-value was $0.58{\pm}0.05$ which showed no statistically significant difference (p = 0.769, 0.614, 0.181). But steep K power was $45.25{\pm}1.36$, and steep K e-value was $0.45{\pm}0.18$ which shows statistically significant difference (p=0.018, 0.027). Conclusions: Consider the comfort, clear vision, dryness for preference fitting investment, 6 students (30%) prefer right eye which is 1 D flatter fitting, 14 students (70%) prefer left eye which is alignment fitting. For rigid gas permeable fitting needed for accurate examination and should prescribe the alignment fitting which is suitable for each cornea.

• Journal of Korean Ophthalmic Optics Society
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• v.11 no.3
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• pp.217-223
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• 2006

The purpose of this study is to provide improvements and standards of trial lens, in a situation that there is a lack of standards of trial lens set that have been used for self-conscious refraction test after helm refraction test at about 5,000 opticians, ophthalmologic clinics and hospitals, and contact-lens shops, that there is a lot of discrepancies between refraction specified and the actual power, and that there is no regulation of optical tolerance error. For the study, opticians who have used Trial lens set were asked to participate in a questionnaire survey through continuing education, and divided into those who have used domestic lens and those who have used imported lens, 5 opticians each for less than 5 years, 5 to 10 years, more than 10 years. The measurement of both refraction specified and the actual diopter was compared to Japan Industrial Standards(JIS T4402). As a result of comparative analysis, more than 80% of respondents have had reliability on the refraction of trial lens they had used, indicating that they have never measured the refraction specified and the actual diopter after buying them. Besides, Korean Industrial Standards(KS P4402) has been imperfect in diopter range since it was legislated in 1979. More than 95% of respondents have been unsatisfied with optometry. Also, it has indicated that refraction error is more frequent in long-term-used trial lens. The conclusion is that it is necessary to standardize trial lens set and that it is required to add lens to lens set provided under KS P4402. Moreover, it is necessary to have supervisory agency for a standardization of trial lens. I hope that both domestic lens and imported lens, as in German and Japan, will be tested to find whether they meet optical tolerance error and standard trial lens will be distributed. Good optic inspection is required for the improvement and management of eye health and optical function, and the same standard trial lens set should be used. whoever is tested. Also, I hope that trial lens set will be specified within standards and tolerance error.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.8
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• pp.353-359
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• 2018

In this study, the performance between subjective refraction and open-field/closed view autorefraction was estimated. We measured the refractive error of early adults aged 18 to 20 years who did not have eye disease. The differences between measurements obtained by subjective refraction and open-field autorefraction for SE, J0, and J45 were $-0.13{\pm}0.53D$ (p=0.17), $+0.33{\pm}0.68D$ (p=0.01), and $+0.13{\pm}0.68D$ (p=0.26), respectively, with only J0 differing significantly. The differences between the measurements of subjective refraction and closed-view autorefraction for SE, J0, and J45 were $-0.30{\pm}0.42D$ (p=0.00), $+0.30{\pm}0.71D$ (p=0.02), and $-0.02{\pm}0.63D$ (p=0.88), respectively, with only SE and J0 differing significantly. The coefficient of accuracy for SE, J0, and J45 components of open-field and closed-view autorefraction were 1.04, 1.33, and 1.34 and 0.83, 1.40, and 1.24, respectively. It is possible to predict the refractive error, which is necessary when deciding on subjective refraction, by measuring the objective refraction of open-field/closed view autorefractors.