• Title/Summary/Keyword: Optimum germination temperature

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Factors Influencing Pollen Germination in vitro of Codonopsis lanceolata (더덕의 花粉發芽에 관한 溫度와 培地條件)

  • 김학현
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.106-110
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    • 1998
  • The present study has been undertake to obtain the fundatmental data of optimum germination condition and to establish storage time for artificial pollinationin Codonopsis lancelata pollen. In vitro condition for germination of freshly collected and stored pollen were examined. The optium temperature for germination of fresh pollen was $25^{\circ}C$. The optium sucrose concentration in the medium ranged from 30 to 40 % and optium pH 6.0% for pollen germination. The rate of pollen germination accelerated considerably in the medium with 1% agar. 30% sucrose, and pH 6. C. lanceolata pollen remained viable for 15 days when stored at 5$^{\circ}C$ with silica gel as desiccant.

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Effects of Environment and Storage Condition on Germination of Astragalus membranaceus (환경 및 저장조건이 황기 종자발아에 미치는 영향)

  • Kim, Young-Guk;Yu, Hong-Seob;Park, Hee-Woon;Seong, Nak-Sul;Son, Seok-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.4
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    • pp.265-268
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    • 2001
  • This experiment was conducted to investigate the effects of temperature, light, seed harvesting time, storage temperature, and period on its germination of Astragalus membranaceus. Optimum germination temperature was ${20{\sim}25^{\circ}C}$ and it was found to be light insensitive seed. The germination rates were not significantly different between 1-year and 2-year old plants, and seed harvesting times. Seeds stored at such cold temperatures as ${-4^{\circ}C\;and\;-20^{\circ}C}$ for 15 months showed over 80% in germination rate at the 6th day after placement. On the other hand, low germination rate of 60% at the 11th day was observed from the seeds stored at $4^{\circ}$ and room temperature.

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Dormancy type and Germination Characteristics of Seeds of Lonicera chrytsantha Turcz. Ex Ledeb (Caprifoliaceae) (각시괴불나무 종자의 휴면유형과 발아특성)

  • Park, Hyung Bin;Ko, Chung Ho;Kim, Sang Yong;Lee, Ki Cheol;Kim, Jae Hyeun;Chung, Jae Min
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.457-462
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    • 2019
  • This study was conducted to investigate seed dormancy type and germination characteristics of Lonicera chrysantha. In imbibition test, the seeds imbibed water readily. Therefore, the seeds did not have physical dormancy (PY). The seeds have undeveloped embryo at seed dispersal and, then the E:S ratio (embryo/seed ratio) increased more than about 46% just before germination. In temperature experiments (5, 15, 20 and $25^{\circ}C$), the seeds did not germinate during incubation at $5^{\circ}C$. At $20^{\circ}C$, the seeds started to germinate from a week after sowing and the final germination was 86.7% at 2 weeks after sowing. At $15^{\circ}C$, the seeds started to germinate at 2 weeks and the final germination rate was 75.0% at 4 weeks after sowing. At $25^{\circ}C$, the seeds started to germinate at a week and the final germination rate was 48.3% at 19 weeks after sowing. Consequently, Optimum temperature of germination is $20^{\circ}C$ that shown highest final germination rate and shortest mean germination time. The seeds germinated all temperature treatments within 4 weeks except to $5^{\circ}C$. Therefore, it is concluded that seeds of L. chrysantha have only morphological dormancy (MD).

Effects of Some Environmental Factors on the Germination of Seeds in Ambrosia artemisiifolia var. elatior (몇가지 환경요인이 돼지풀의 종자발아에 미치는 영향)

  • 김종홍;김원희;차승희
    • The Korean Journal of Ecology
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    • v.25 no.2
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    • pp.93-100
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    • 2002
  • In order to analyze the life cycle of A. artemisiifolia var. elation dormancy and some environmental factors inducing germination of the seeds were examined. The results were as follows : Dormancy of fertile seeds was broken in part within a month after seed collection in case of adequate moisture and alternating temperature was also effective in breaking dormancy. The temperature range, which allow germination was 12℃ ∼ 32℃. Optimum temperature for germination was 24℃. The seed of A. artemisiifolia var. elatior was light-independent. The difference of storage period appeared to have no particular effect on the viability of seeds at any time during the 9-month storage period. In the increasing temperature(IT) regime, A artemisiifolia var. elatior seeds started to germinate at 16℃, showing the higher temperature the greater germination rate, the final germination percentage was 99.34%. On the other hand, in the decresing temperature(DT) regime, seeds began to germinate at 20℃ with the 1.34% germination. An induced dormancy occurred at 12℃ making the 5.34% fecal germination in the DT regime. Low temperature was more effective to break dormancy than higher temperature Seeds of A. artemisiifolia var. elatior seems to be germinated in mid to late autumn or germination delayed until following spring. The above results suggest these variation of germination response in diverse environmental factors seems to be a physiological strategy to maintain their existence and to reproduce in the extreme thermal variation.

Optimum Chilling Terms for Germination of the Dehisced Ginseng(Panax ginseng C. A. Meyer) Seed (개갑 인삼종자의 발아 적정 저온감응기간)

  • 권우생;이장호;이명구
    • Journal of Ginseng Research
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    • v.25 no.4
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    • pp.167-170
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    • 2001
  • Experiments were conducted to study the optimum chilling period for breaking physiological dormancy of dehisced ginseng(Panax ginseng C. A. Meyer) seed. Embryo of ginseng seed is too small to be noticed with naked eyed on harvesting time. Embryo grew to half size of endosperm after seeds were stratified for 3 months. It takes 6 months for this embryo to reach the size enough for germination. And it grew faster indoors than outdoors. Dehisced ginseng seed with full-size embryo did not germinate at room temperature and required chilling treatment for 75days in outdoors and 90 days in cold chamber. While seed receiving sufficient chilling treatment were left to be in room temperature, the chilling effect decreased remarkably.

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Comparison of Seed Germination Response to Temperature by Provenances in Fraxinus rhynchophylla (채취산지별 물푸레나무 종자의 온도에 대한 발아반응 비교)

  • Choi, Chung Ho;Seo, Byeong Soo;Tak, Woo Sik;Cho, Kyung Jin;Kim, Chang Soo;Han, Sang Urk
    • Journal of Korean Society of Forest Science
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    • v.97 no.6
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    • pp.576-581
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    • 2008
  • The germination responses of Fraxinus rhynchophylla seeds collected from four provenances to constant temperature were investigated over the range $5{\sim}35^{\circ}C$. Difference among seeds in percentage and rate of germination and cardinal temperatures was observed. The seeds from Inje had high germination percentage at low temperature ($5{\sim}15^{\circ}C$) whereas those from Gangneung had high germination percentage at high temperature ($30{\sim}35^{\circ}C$). Three cardinal temperatures viz., the base ($T_b$), the maximum ($T_m$) and the optimum ($T_o$) for germination percentage and germination rate varied among four provenances. $T_b$, $T_m$ and $T_o$ for F. rhynchophylla seed germination as estimated by the quadratic models were the lowest in Inje while those were the highest in Gangneung. The cardinal temperatures ($T_b$, $T_m$ and $T_o$) were estimated by linear sub- and supra-optimal models for germination rate as a function of temperature response. $T_b$ was the lowest in Hoengseong while that was the highest in Gangneung. $T_m$ and $T_o$ were the lowest in Inje while those were also the highest in Gangneung. That is, the seeds from the provenance where the annual mean temperature was high had the higher cardinal temperatures ($T_b$, $T_m$ and $T_o$) as compared to seeds from the provenance where the annual mean temperature was low.

Temperature Effect on Seed Germination and Seedling Growth of Medicinal Umbelliferae Plants

  • Chio, Seong-Kyu;Chon, Sang-Uk
    • Plant Resources
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    • v.3 no.1
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    • pp.66-70
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    • 2000
  • Umbelliferae medicinal crops have low germination under natural condition. Special objective of this study is to investigate morphological characteristics of seeds and effects of environmental factors such as light and temperature on their germination and seedling growth in Umbelliferae plants, using them as oriental medicine materials. Fruits of Umbelliferae are bi-partite type and form 2 seeds. Their oil canals and funiculus or carpaphore has apparently specific shape. Seed shape of Buplerum falcatum, Ligusticum acutilobum and Foeniculum vulgare are oblong, Ledeboutiella seseloides, Angelica dahurica, Angelica gigas elliptic, and Ostericum koreanum ovate. Color of seed coats varied from yellow to dark brown. Seed size was 3.2 to 6.9 mm in length, 1.7 to 4.9 mm in width. One thousand-seed weight of Buplerum falcatum was lowest(at 1,660 mg) and Ledebouriella seseloides and Angelica gigas were relatively highest(at 3,970 mg) having larger size than any others. Optimum temperatures for seed germination and seedling growth ranged from 20 to $25^{\circ}C$ and especially temperature at $25^{\circ}C$ improved seed germinations of Ledebouriella seseloides, Angelica dahurica and Ligusticum acutilobum regardless of light condition.

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Priming Effect of Rice Seeds on Seedling Establishment under Adverse Soil Conditions

  • Lee, Suk-Soon;Kim, Jae-Hyeun;Hong, Seung-Beam;Yun, Sang-Hee;Park, Eui-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.3
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    • pp.194-198
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    • 1998
  • An experiment was carried out to find out the priming effects of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) on. the seedling establishment and early emergence under excess soil moisture conditions. Seeds were primed by soaking in -0.6 MPa polyethylene glycol (PEG) solution at $25^{\circ}C$ for 4 days. The primed seeds were sown in soils with various soil moistures (60, 80, 100, 120, and 140% field capacity) at 17 and $25^{\circ}C$, respectively. Germination and emergence rates, plumule height, and radicle length of primed seeds were higher than those of untreated seeds at any soil moisture and temperature examined. The time from planting to 50% germination ($T_{50}$) of primed seeds was less than that of untreated seeds by 0.9~3.7 days. Germination rate, emergence rate, plumule height, and radicle length were highest at the soil moisture of 80% field capacity among the soil moistures. Priming effects of rice seeds on germination and emergence rates were more prominent under the unfavorable soil moistures (60, 100, 120, and 140% field capacity) than those under the optimum soil moisture condition (80% field capacity). However, priming effects on seedling growth were greater at near optimum soil moisture compared with too lower or higher soil moistures. Therefore, these findings suggest that priming of rice seeds may be a useful way for better seedling establishment under the adverse soil conditions.

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Selection of Binder and Solid Materials for Pelleting Welsh Onion(Allium fistulosum L.) Seeds (파 펠렛종자 생산을 위한 접착제 및 피복재료 선발)

  • 강점순
    • Journal of Life Science
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    • v.12 no.6
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    • pp.721-730
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    • 2002
  • This study was carried out to select new pelleting binder and material for Welsh onion seeds. The optimum treatments of the various plant growth regulators to improve seed germination of the Welsh onion was also estimated. There were no significant effects of growth regulators on the germination percentage, but germination was faster according to the number of days to 50% of the final germination ($T_{50}$) and the mean number of days to germination (MDG) than those of the control. Germinability was increased when the seeds were soaked in gibberellic acid ($GA_3$) solution for 24hrs, even though there was no synergy effect on the germinability when $GA_3$ was mixed with 6-benzylaminopurine (BAP). The optimum treatment for improving germination of Welsh onion was observed when the tested seeds was soaked in 500 $\mu$M of $GA_3$ at $20^{\circ}C$ for 24hrs. Also, when the seeds soaked in the aforementioned treatment, the rate of germination was increased at lower temperature than at $20^{\circ}C$, the optimal temperature. The percentage and the speed of seed germination depended on the kinds of pelleting binder and their concentration. It showed that the higher the concentration of the binder for seeds pelleting, the lower the percentage of seed germination. Among the pelleting binder, polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) were the best binders for seed pelleting, because seed germination using those binder did not affect on the concentration of binder. On the other hand, Carboxymethy cellulose (CMC) and methyl cellulose (MC) severely inhibited the seed germination The germinability was also different arcording to the pelleting materials. Among the different 58 pelleting materials, kaoline alone, the mixture of bentonite and kaoline, the mixture of bentonite, calcium carbonate and diatomaceous earth #300 were found as the best pelleting materials for welsh onion seeds.

A Study for Use of Wild Rhododendron mucronulatum for. albiflorum as Landscape plant (야생 흰진달래의 조경식생화를 위한 연구)

  • 이기의;이우철;조현길;유시철
    • Journal of the Korean Institute of Landscape Architecture
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    • v.18 no.4
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    • pp.73-85
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    • 1991
  • Rhododendron mucronulatum for. albiflorum, native species is a shrub that has white flowers on May to June, and rare species endangered by people's rash digging or cutting. But its physiological ecological characteristics and propagation method are not being known at all. Therefore, this study was executed to utilize this species as the planting material for landscaping by analysing its habitat environment and growth form, and also experimenting its seed and vegetative propagation, and it field culture and utilization. The results are as follows; 1. The elevation, gradient and direction of this species were 295-1,350m, 10-36$^{\circ}$, northwest respectively. It was found that the species is shade-liking plant that grows under forest cover of average 51.33%. 2. The soil pH and water content of its habitat were 5.4, 25.41% respectively. The organic matter content was 6.29% that was higher than 3.2%, the average organic matter content of forest soil in Korea. 3. Representative plant community within which this species was living was Quercus mongolica community, and its main neighboring species were Lindea obtusiloba, Fraxinus sieboldiana, Rhus trichocarpa, Rhododendron Schlippenbachii, Rododendron mucronulatum. 4. The leaf length and width of this species were 39.18mm, 12.60mm respectively. This result showed that generally its leaf size was larger than that of R. micranthum, R. yedoense var. poukhanense and R. mucronulatum var. ciliatum and smaller than that of R. mucronulatum and R. schlippenbachii. 5. The whole size of its pollen was, as 59${\times}$61$\mu\textrm{m}$, the largest of plants of Rhododendron family including R. mucronulatum and R. mucronulatum var. ciliatum. 6. The result of seed germination experiment at intervals of 5$^{\circ}C$ from 15$^{\circ}C$ to 30$^{\circ}C$ presented the highest germination rate of 94.7% at 20$^{\circ}C$ numerically, but high percent germination at all temperature levels without significant difference. And the seed of this plant proved to be sun-liking seed at requiring dormancy in germination. 7. Through seed germination experiment by treatment of growth regulators such as GA. Thiourea and Kinetin under dark condition, it was found that the effect of GA treatment on germination increase and acceleration was the highest. 8. In greenwood cutting, rooted rate by treatment of various concentration of IBA and NAA on clay and vermiculite bed was not wholly high, but 100ppm plots of both IBA and NAA of clay bed showed relatively good rooted rate. 9. As result of field culture experiment for finding out optimum growth temperature and light intensity, growth conditions such as height, number of leaves, fresh weight and chlorophyll contents were the best at night/day temperature of 20/25$^{\circ}C$ and under 1/2sun. Also, the photosynthetic rate was the highest at 25$^{\circ}C$. Accordingly, it was found that optimum temperature and light intensity for growth of this plant are 25$^{\circ}C$ (day temperature), 50% of natural light respectively.

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