• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1497-1504
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• 2009

GC/MS analysis of catecholamines (CAs) in biological sample may produce poor reproducible quantitaion when chemical derivatization is used as the technique to form a volatile derivative. Significant quantities of the side products can be formed from CAs with primary amine during the derivatization reaction under un-optimized conditions. We have tested various chemical derivatization techniques in an attempt to find an optimum derivatization method that will reduce side product formation, enable to separate several catecholamine derivatives in GC chromatogram, and obtain significant improvement of detection sensitivity in GC/MS analysis. Whereas several derivatization techniques such as trimethylsilylation (TMS), trifluoroacylation (TFA), and two step derivatization methods were active, selective derivatization to form O-TMS, N-heptafluorobutylacyl (HFBA) derivative using N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) and N-methyl-bis(heptafluorobutyramide) (MBHFBA) reagents was found to be the most effective method. Moreover, this derivative formed by selective derivatization could provide sufficient sensitivity and peak separation as well as produce higher mass ion as base peak to use selected ion in SIM mode. Calibration curves based on the use of an isotopically labeled internal standard show good linearity over the range assayed, 1 ~ 5000 ng/mL, with correlation coefficients of > 0.996. The detection limits of the method ranged from 0.2 to 5.0 ppb for the different CAs studied. The developed method will be applied to the analysis of various CAs in biological sample, combined with appropriate sample pretreatment.

• Fashion & Textile Research Journal
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• v.12 no.4
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• pp.467-476
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• 2010

The purpose of this study was to develop a plausible methodology based on experimental data how to set up darts and split lines on 3D parametric body dressed with tight-fit garment. The results were as following: Through the process of making convex hull, the concave parts were straightened to make a convex hull, especially in the center part of bust, under breast part and scapular part. To figure out the optimum positions of darts and split lines, the inflection points of curve ratio were searched along the horizontal polylines of waist and bust. This procedures produced reliable results with low deviation. Using Rapidform, CATIA and Unigraphics, six patches of bodice patterns were drawn and aligned. Paired t-test results showed the outline and area between 3D surface and 2D were not significantly different, meaning this method could be adaptable when flattening 3D surfaces. The amount of waist dart measured on the pattern showed that the highest portion was allocated on 2nd dart(back), followed by 1st dart(back), 1st dart(front), 2nd dart(front)/side dart, and center back dart. A series of findings suggested that curve ration inflection point could be used as a guide to set up darts and split line on 3D parametric model with low deviation.

• The Korean Journal of Zoology
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• v.4 no.1
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• pp.7-12
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• 1961

1) Respiratory metabolism patterns and its enzyme systems in the gill tissue of the fresh water mussels, Cristaria plicata were investigated through the examination on the effects of respiratory enzyme inhibitors, (KCN, NAF) and succinoxidase assay, while studying the effects of neutral salts (NaCL, KCL, CaCl2) and pH on oxygen consumption of the gill tissue. 2) In the limited concentration of KCL (0.3mM) and NaCl (0.4mM) solutions, oxygen consumption of the intact gill tissue was accelerated, but in CaCl2(0.5mM) solution, it showed no significant effect. The oxygen consumption was gradually decreased at the above concentrations of these limitations. The optimum pH for the respiration of the gill was 7.3. 3)Cyanide in 10-8M solution inhibited 88.8% of the respiration of the intact gill tissue. Methylene blue accelerated the respiration of the noral gill tissue, and slightly but significantly reversed the cyaniide poisoned respiration. 4)Oxygen consumption of the gill homogenate was apparently increased by the mixed addition of succinate, cytochrome c and activators (AlCl3 and CaCl2). This results suggested that succinoxidase system acts on the respiratory pattern of the gil tissue. 5) It was able to recognize that the enolase, which acts on the anaerobic glycolytic system, participated in the tissue respiration of the gill for NaF in 5$\times$10-2 M solution inhibited 55.5% of the respiration of the same intact tissue.

• Journal of the Korean Society of Propulsion Engineers
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• v.5 no.1
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• pp.26-33
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• 2001

Thrust vector control system is control device which is mounted exit of the nozzle to generate pitch, yaw and roll directional force by deflecting flow direction of the supersonic jet from the nozzle. By obtaining control force, jetvane which is exposed in jet flow is working thermal and aerodynamic load. Axial thrust loss and side thrust is affected by shock patterns and interactions between jetvanes according to jetvane geometry and turning angle. In this study, we designed 6 types of jetvane to evaluate pitch, yaw and roll characteristics of ietvane in supersonic flow, and perform the cold flow test in range of turning angles of jetvanes between $0^{\cire}$ and $25^{\cire}$ by $5^{\cire}$ respectively. Also, calculation is going side by side to analyse flow interaction. Results show that there is no interactions between jetvanes upto turning angle 20$^{\circ}$, chord and lead length ratio is very important parameter to aerodynamic performance and maximum thrust loss is appeard to 17% of axial thrust in roll directional control.

• Wind and Structures
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• v.22 no.2
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• pp.161-184
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• 2016

Long-span suspension bridges have evolved through the years and with them, the bridge girder decks improved as well, changing their shapes from standard box-deck girders to twin box and multi-box decks sections. The aerodynamic characteristics of the new generation of twin and multiple-decks are investigated nowadays, to provide the best design wind speeds and the optimum dimensions such bridges could achieve. The multi-box Megane bridge deck is one of the new generation bridge decks, consisting of two side decks for traffic lanes and two middle decks for railways, linked between them with connecting beams. Three-dimensional CFD simulations were performed by employing the Large Eddy Simulation (LES) algorithm with a standard Smagorinsky subgrid-scale model, for $Re=9.3{\times}10^7$ and angles of attack ${\alpha}=-4^{\circ}$, $-2^{\circ}$, $0^{\circ}$, $2^{\circ}$ and $4^{\circ}$. Also, a wind tunnel experiment was performed for a scaled model, 1:80 of the Megane bridge deck section, for $Re=5.1{\times}10^5$ and the aerodynamic static coefficients were found to be in good agreement with the results obtained from the CFD-LES model. However the aerodynamic coefficients determined individually, from the CFD-LES model, for each of the traffic and railway decks of the Megane bridge, varied significantly, especially for the downstream traffic deck. Also the pressure distribution and the effect of the spacing between the connecting beams, on the wind speed profiles showed a slight increase in turbulence above the downstream traffic and railway decks.

• Journal of the Korean Geotechnical Society
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• v.18 no.5
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• pp.123-132
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• 2002

The purpose of this study is to predict the shear behavior of the weathered mudstone soil using dynamic neural network which mimics the biological system of human brain. SNN and RNN, which are kinds of the dynamic neural network realizing continuously a pattern recognition as time goes by, are used to predict a nonlinear behavior of soil. After analysis, parameters which have an effect on learning and predicting of neural network, the teaming rate, momentum constant and the optimum neural network model are decided to be 0.5, 0.7, 8$\times$18$\times$2 in SU model and 0.3, 0.9, 8$\times$24$\times$2 in R model. The results of appling both networks showed that both networks predicted the shear behavior of soil in normally consolidated state well, but RNN model which is effective fir input data of irregular patterns predicted more efficiently than SNN model in case of the prediction in overconsolidated state.

• Journal of Microbiology and Biotechnology
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• v.17 no.2
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• pp.262-270
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• 2007

Of the twelve lytic bacteriophages recovered from five different fermenting cucumber tanks that were inoculated with Pediococcus sp. LA0281, a lytic phage, ${\phi}ps05$, was characterized in the present study. The plaques were mostly clear and round-shaped on the lawn of starter strain, indicating lytic phage. Overall appearance indicated that it belongs to the Siphoviridae family or Bradley's group B1, with a small isometric head and a flexible noncontractile tail with swollen base plate. The average size was found to be 51.2 nm in head diameter and 11.6 nm wide ${\times}$ 129.6 nm long for the tail. The single-step growth kinetics curve showed that the eclipse and the latent period were 29 min and 34 min, respectively, and an average burst size was calculated to be 12 particles per infective center. The optimum proliferating temperature ($35^{\circ}C$) was slightly lower than that of cell growth ($35\;to\;40^{\circ}C$). The structural proteins revealed by SDS-PAGE consisted of one main protein of 33 kDa and three minor proteins of 85, 58, and 52 kDa. The phage genome was a linear double-stranded DNA without cohesive ends. Based on the single and double digestion patterns obtained by EcoRI, HindIII, and SalI, the physical map was constructed. The overall size of the phage genome was estimated to be 24.1 kb. The present report describes the presence of a lytic phage active against a commercial starter culture Pediococcus sp. LA0281 in cucumber fermentation, and a preliminary study characterizes the phage on bacterial successions in the process of starter-added cucumber fermentation.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.06a
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• pp.97-98
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• 2007

A stoichiometric. mixture of evaporating materials for $CuGaSe_2$ single crystal thin films was prepared from horizontal electric furnace. Using extrapolation method of X-ray diffraction patterns for the polycrystal $CuGaSe_2$, it was found tetragonal structure whose lattice constant $a_0$ and $c_0$ were $5.615\;{\AA}$ and $11.025\;{\AA}$, respectively. To obtain the single crystal thin films, $CuGaSe_2$ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the hot wall epitaxy (HWE) system. The source and substrate temperatures were $610^{\circ}C$ and $450^{\circ}C$, respectively. The crystalline structure of the single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). The carrier density and mobility of $CuGaSe_2$ single crystal thin films measured with Hall effect by van der Pauw method are $4.87{\times}10^{17}\;cm^{-3}$ and $129\;cm^2/V{\cdot}s$ at 293 K, respectively. The temperature dependence of the energy band gap of the $CuGaSe_2$ obtained from the absorption spectra was well described by the Varshni's relation, $E_g(T)\;=\;1.7998\;eV\;-\;(8.7489\;{\times}\;10^{-4}\;eV/K)T^2/(T\;+\;335\;K)$.

• Journal of Korean Society of Transportation
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• v.27 no.5
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• pp.195-208
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• 2009

A network design problem (NDP) formulated as a mathematical program is generally used to find an optimum value to minimize or to maximize some objectives such as total travel time, social benefit, or others. NDP has, however, some limits of describing components of travel patterns like activities and trip generation due to its modeling simplicity, and also it has difficulty in including attributes of regional planning. In order to cope with such limits, this paper extends NDP to the urban planning field and proposes a mathematical program which can describe the interactions between urban social activities and transportation planning. Based on this model the authors try to optimize both urban activities and the transportation system. The model developed in this paper is tested to assess its application with a real-size regional transportation network.

• Journal of Korean Society of Water and Wastewater
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• v.13 no.3
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• pp.101-106
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• 1999

This research aims to compare immobilized catalysts prepared by various methods and determine suitable $TiO_2$ catalyst for water purification. Sol-gel method by Anderson and powder coation method by Tanaka ate famous in the methods to immobilize catalyst. Therefore, the $TiO_2$ catalyst for this research was prepared by sol-gel method and powder coating method. Its structure was tested by X-ray diffractometer (XRD), Scanning electron microseope (SEM). Durability of a catalyst-support couple in an solution was investigated. too. Experimental results were summarized as following; i) Optimum ratio of Ti : $H_2O$ : $H^+$ to obtain stable sol was 1 : 10 : 0.1 and the XRD patterns of $TiO_2$ film immobilized by sol-gel method which were fired at $700^{\circ}C$ showed that the catalyst had an anatase structure. ii) The particle size of $TiO_2$ prepared by sol-gel method was less than $5{\mu}$, but it was observed that coated side was not unifiom. iii) Sol-gel method was very effective to obtain $TiO_2$ catalyst of thin film, but spreadability and durability of a catalyst-support couple in a solution were than $TiO_2$ film immobilized by powder coating method. iv) The particle size of $TiO_2$ immobilized by powder coating method was a little larger than it prepared by sol-gel method, but spreadability and uniformity of $TiO_2$ film and durability of a catalyst-support couple in a solution were better than it immobilized by sol-gel method.