• Korean Journal of Food Science and Technology
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• v.18 no.4
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• pp.270-277
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• 1986

Ficin, a proteolytic enzyme in Fig latex, was extracted and purified with using ammonium sulfate and CM-cellulose column chromatography, respectively, and studied for its chemical properties. The disc gel electrophoresis showed one major and three minor bands for $(NH_4)_2SO_4$ extract and only one band showed after CM-cellulose chromatography. The optimum conditions for ficin activity was found to be pH 7.0 and $50^{\circ}C$. The amino acids composition of the purified ficin were 21.8% as acidic, 3.5% as basic and 74.7% as neutral amino acids. The amino acids analysis indicated that the ficin was composed of 174 amino acids residue having molecular weight of 19,500.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.2
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• pp.355-362
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• 2015

In this study, we have fabricated 9.385[GHz] circulator that is composed of WR112 waveguide and Ferrite for X-band radar. For designing Ferrite, B/R mode(Below Resonance mode) was used and calculated the condition of 120 degree rotation of the electric field in Ferrite and calculated internal DC magnetic field and external DC magnetic field. Also, dielectric materials of the same shape with Ferrite was filled between two Ferrite for improving the performance of the circulator, including impedance matching, bandwidth, quality factor, insertion loss. To obtain optimum shape of the Ferrite and dielectric material, we used CST MWS. Simulation result of the circulator is that 1.02 : 1 VSWR, -40dB isolation, 0.2dB insertion loss and measurement result is that 1.03 : 1, -38dB, 1.2dB at 9.385[GHz]. We can get good agreement at isolation and VSWR, but insertion loss was 1 dB great than simulation result.

• Journal of Navigation and Port Research
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• v.26 no.5
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• pp.525-528
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• 2002

The proposed EMC filter composed with feed-through capacitors and ferrite beads of high permeability was prepared which satisfy the EMC standard for a wide-band noise signal in the frequence of 10 MHz to 1.5 GHz in power supply line. The optimum structure of ferrite bead was found by calculating the load effect of ferrite beads. As a result, the filter showed excellent differential- and common-mode noises filtering characteristics above 30dB in the frequency band from 10 MHz to 1.5 GHz. The immunity characteristics are improved more than 10 to 30 dB over the frequency band from DC to 1.8GHz.

• Fisheries and Aquatic Sciences
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• v.6 no.1
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• pp.7-12
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• 2003

Chitinase and chitobiase produced by Aeromonas sp. J-5003 were purified and characterized. The chitinase was purified to 19.4 folds by gel chromatography and ion-exchange chromatography with the overall yield of $2.2\%$ and the specific activity of 93.1 unit/mg. The purified enzyme showed a single band on SDS-PAGE with MW 54kDa. The optimum pH and temperature of the purified chitinase were 7.0 and $37^{\circ}C$, respectively, and this enzyme stable in the range of pH 6.0 to 10.0 below $37^{\circ}C$. $Mg^{2+},\;Ca^{2+}\;and\;Na^+$ slightly stimulated the chitinase activity. However, $Hg^{2+}\;and\;Fe^{3+}$ inhibited chitinase activity. The chitobiase was purified by Sephacryl HR-l00 gel chromatography and DEAE-Sephadex A-50 ion-exchange chromatography with 33.5 purification folds and $4.3\%$ yield. The purified enzyme showed a single band with MW 63 kDa. The optimum pH and temperature of the purified chitobiase were 7.0 and $37^{\circ}C$, respectively. And this enzyme was stable in the range of pH 6.0 to 9.0 and at the temperature below $37^{\circ}C$. The enzyme activity was increased by $Mn^{2+}$, but it was inhibited by $Ag^+$.

• Applied Chemistry for Engineering
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• v.25 no.6
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• pp.577-580
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• 2014

Indium hydroxy sulfide ($In(OH)_xS_y$) as a cadmium (Cd)-free buffer layer for $CuInGaSe_2$ (CIGS) solar cells was prepared by the chemical bath deposition (CBD) and the reaction time was optimized. The band gap energy and transmittance data alongside the thickness results from the direct observation with focused ion beam system (FIB) could be a powerful tool for optimizing the conditions. In addition, X-ray diffractometer (XRD), X-ray photoelectron microscopy (XPS), and scanning electron microscope (SEM) were also employed for the layer characterization. The results indicated that the optimum reaction time for $In(OH)_xS_y$ buffer layer deposition by CBD was 20 min at $70^{\circ}C$ under the conditions employed. At the optimum conditions, the buffer layer thickness was near 57 nm and the band gap energy was 2.7 eV. In addition, it was found that there was no XPS peak shift in between the buffer layers deposited on molybdenum (Mo)/glass and that on CIGS layer.

• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.409-416
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• 1992

The optimum cultural temperature and time for the pullulanase production by Bacillus cereus were $15^{\circ}C$ and 72 hrs, respectively. The addition of casein, nutrient broth and egg albumin to the basal medium, respectively, increased greatly the enzyme production. The enzyme was purified by ammonium sulfate fractionation, CM-cellulose and DEAE-cellulose column chromatographies. The specific activity of the purified enzyme was 29.09 U/mg protein and the yield of enzyme activity was 17.1% The purified enzyme showed a single band on polyacrylamide disc gel electrophoresis and its molecular weight was estimated to be 61,000 by SDSpolyacrylamide disc gel electrophoresis. The isoelectric point for the purified enzyme was pH 7.0. The optimum temperature and pH were $40^{\circ}C$ and 6.5. The purified enzyme was stable below $35^{\circ}C$ and in the pH range of 6.5-11.0. It was greatly inhibited by $Ag^{+}$, $Hg^{2+}$ and $Zn^{2+}$, and its thermal stability was increased by the addition of $Ca^{2+}$ Among various substrates, pullulan was favorably hydrolyzed by the purified enzyme and the hydrolysis product 011 pulluIan was maltotriose.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.6
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• pp.914-919
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• 2000

A simple orthogonal mode transducer(OMT) for X-band has been proposed in this paper. This OMT is designed such that a polarized wave can be guided in a direction using several thin guiding posts, and the other orthogonally polarized wave can be guided in the other direction using other thin guiding posts. The OMT consists of a T-shaped waveguide section with guiding posts, two tapering waveguide arms, and has three flanges for connection with two adapters and an antenna. The optimum numbers and the optimum positions of the guiding posts were obtained numerically using the HFSS CAD tool. The isolation between two polarizations in a manufactured OMT is higher than 42.8 dB and the reflection from the OMT is lower than -17.5 dB at the frequency band of 9.5 GHz

• Journal of Navigation and Port Research
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• v.27 no.4
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• pp.359-366
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• 2003

The paper describes on the construction and analysis of marine casualty numerical D/B (N-D/B) to implement Korean MArine Casualty Forecasting System (K-MACFOS). The main target of K-MACFOS is to broadcast the prediction number and risk level of marine casualties as like daily weather forecasting. The data relating to a total of 724 ship casualties in the west-southern sea area (33oN∼35oN, 124oE∼127oE) of Korean peninsula for 11 years (1990∼2000) have been compiled and converted into quantitative data with 14 numeric conversion scales. Through the statistical analysis using contour-map visualization, the usability of N-D/B and the casualty features of the target sea areas are discussed. In addition, the optimum year-band selection method is also proposed to provide correct N-D/B analysis and precise prediction of the number of marine casualties.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.1
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• pp.28-35
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• 2010

In this paper, we designed and fabricated a ultra-wideband(UWB) bandpass filter with a good performance of a stopband, and a notched band in passband. The transformed equivalent circuit of the highpass filter was realized by distributed element. A wide-passband with 3-dB fractional bandwidth of more than 100 % was achieved by using optimum response of the HPF. For improving lower and upper stopband characteristic, a cross coupling between feed lines was employed, which was analyzed by desegmentation technique. In order to reject interference of Wireless LAN and Hyper LAN(5.15~5.825 GHz), the narrow notched(rejection) band was realized by a spurline. The fabricated BPF indicated the passband from 3.1 to 10.55 GHz and the flat group delay of less than 0.94 ns over the entire passband except the rejection band. The filter shown sharp attenuation both inside and outside the band and notched band from 5.2 to 6.12 GHz.

• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.4
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• pp.397-402
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• 1984

As a basic research for inhibition of enzymatic browning of apple wine, polyphenol oxidase (EC 1.10.3.1) from apple (Jonathan) was extracted, partially purified, and some properties of the enzyme and changes o( active bands by heat treatment were investigated. Optimum conditions for the enzyme reaction were pH6.5 and temperature of $30^{\circ}C$, and o-diphenol was the main substrate for the enzyme. Approximately 35% and 15% of initia lpolyphenol oxidase activity remained after heating at $60^{\circ}C$ and $70^{\circ}C$ for 1 hour, respectively. About 0.5mM of the inhibitor such as sodium metabisulfite, cysteine and ascorbic acid was required for effective inhibition of the enzyme reaction. However, EDTA was found to be a very poor inhibitor. Ethanol did not affect the enzyme activity. The number of active bands of polyphenol oxidase from apple(Jonathan) was found to be four, but two bands and one band were observed after heating at $60^{\circ}C$ and $70^{\circ}C$ for 1 hour, respectively, which showed a significant difference in thermal stability among active bands.