Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 18 Issue 4
- /
- Pages.270-277
- /
- 1986
- /
- 0367-6293(pISSN)
Isolation and Purification of Ficin from Fig Latex
무화과(無花果)에서 Ficin의 분리(分離) 및 정제(精製)
- Kim, Jun-Pyoung (Department of Food Science and Technology, Chung-Ang University) ;
- Suh, Jai-Sin (Department of Food Science and Technology, Chung-Ang University) ;
- Kim, Jung-Sook (Department of Home Economics Chung-Ang University)
- Published : 1986.08.01
Abstract
Ficin, a proteolytic enzyme in Fig latex, was extracted and purified with using ammonium sulfate and CM-cellulose column chromatography, respectively, and studied for its chemical properties. The disc gel electrophoresis showed one major and three minor bands for
Ammonium sulfate 염분획과 CM-cellulose chromatography로 Fig latex에서 ficin을 분리하여 다음과 같은 결과를 얻었다. 분리한 crude ficin을 전기영동한 결과 main band 와 3개의 minor band로 나타났으며 main peak의 정제에서 얻은 ficin은 단일 band로 나타났다. 분리한 ficin의 pH 및 열에 대한 영향을 PH7.0,
Keywords