• Journal of Life Science
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• v.20 no.7
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• pp.1027-1033
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• 2010

Liriope platyphylla has traditionally been used in Korea and China as a therapeutic drug for the treatment of coughing, sputum, neurodegenerative disorders, obesity, and diabetes. In an effort to assess the functions of a novel extract from Liriope platyphylla in diabetes therapy, the insulin secretion abilities of 10 extracts were screened via measurements of insulin concentration in the culture supernatant using an Insulin ELISA kit. The results of this assay showed the highest levels of insulin in the LP9M80-H treated group, followed by the LP-H, LP-M, LP-E and LP9M80-C treated groups, whereas other extracts did not induce insulin secretion in the HIT-T15 cells. However, the extracts capable of stimulating insulin secretion simultaneously evidenced high apoptotic activity as compared with other extracts. Therefore, one of these extracts, LP9M80-H, was initially selected as the optimal candidate for a therapeutic drug and its optimal concentration was determined. The results of the ELISA and MTT assay demonstrated that a concentration of approximately 100-125 ug/ml of LP9M80-H was optimal with regards to cell viability and insulin secretion in the HIT-T15 cells. These results suggest that LP9M80-H could be considered as an excellent candidate for a diabetes-therapeutic drug that could induce insulin secretion in pancreatic $\beta$-cells.

• Korean Journal of Agricultural and Forest Meteorology
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• v.18 no.4
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• pp.208-220
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• 2016

Automatic Mountain Meteorology Observation System (AMOS) is an important ingredient for several climatological and forest disaster prediction studies. In this study, we select the optimal sites for AMOS in the mountain areas of Honam and Jeju in order to prevent forest disasters such as forest fires and landslides. So, this study used spatial dataset such as national forest map, forest roads, hiking trails and 30m DEM(Digital Elevation Model) as well as forest risk map(forest fire and landslide), national AWS information to extract optimal site selection of AMOS. Technical methods for optimal site selection of the AMOS was the firstly used multifractal model, IDW interpolation, spatial redundancy for 2.5km AWS buffering analysis, and 200m buffering analysis by using ArcGIS. Secondly, optimal sites selected by spatial analysis were estimated site accessibility, observatory environment of solar power and wireless communication through field survey. The threshold score for the final selection of the sites have to be higher than 70 points in the field assessment. In the result, a total of 159 polygons in national forest map were extracted by the spatial analysis and a total of 64 secondary candidate sites were selected for the ridge and the top of the area using Google Earth. Finally, a total of 26 optimal sites were selected by quantitative assessment based on field survey. Our selection criteria will serve for the establishment of the AMOS network for the best observations of weather conditions in the national forests. The effective observation network may enhance the mountain weather observations, which leads to accurate prediction of forest disasters.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.35 no.6
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• pp.609-616
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• 2017

National Geographic Information Institute has established a plan that preoccupies UCPs (Unified Control Points) at 2~3km intervals in urban areas by considering the distance between existing UCPs by satellite images and aerial photographs in 2015. In this study, we discussed the method of selecting the locations of optimal UCPs by simulating GPS reception environment in candidate sites for UCPs using GIS. For this purpose, we selected new candidate sites for installing UCPs using satellite images and aerial photographs, and analyzed the GPS reception environment by calculating the visibility distance from buildings around UCPs using GIS skyline analysis. The number of and the arrangement of visible satellites that are capable of GPS satellite reception from the viewpoint of sky view were showed by GIS skyline analysis. Quality evaluation results of GPS observation data were compared with average PDOP calculated from hourly PDOP and TEQC in two points of Sungkyunkwan University during 8 hours. As a result of GPS reception environment using GIS, if the PDOP increases, the data acquisition rate is lowed, and the multipath error and the cycle slip are increased. Thus, this study verified that the quality of GPS observation data can be secured by constructing three-dimensional spatial information and simulating PDOP when preoccupying multiple UCPs using GIS.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.779-783
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• 2006

The adiponectin gene is known to be involved in the regulation of energy homeostasis involving food intake, carbohydrate and lipid metabolism. Human adiponectin gene polymorphisms have been recently reported to be associated with obesity, insulin sensitivity and the risk of type 2 diabetes. The present study was carried out to investigate the porcine adiponectin gene as a candidate gene for fat deposition and carcass traits. A mutation of A178G of the porcine adiponectin gene that resulted in substitution of the amino acid Isoleucine to Valine was identified. AcyI PCR-RFLP was used to detect the polymorphism of the genotypes in five different pig populations (Large White, Landrace, Duroc, Chinese breeds Meishan and Qingping). The A allele frequency was significantly higher among subjects from Chinsese lard type breeds, while the G allele was the only one present in those from Western lean type breeds. To determine if there was an association of the polymorphism with phenotypic variation, the mutation was tested in 267 pigs of the "Large $White{\times}Meishan$" F2 resource population. The results of association analyses showed significant associations of the genotypes with fat deposition and carcass traits. Allele G was significantly associated with increase in loin eye height, loin eye area and lean meat percentage and bone percentage, and decrease in fat mean percentage, ratio of lean to fat, shoulder fat thickness, 6-7 rib fat thickness, thorax-waist fat thickness and buttock fat thickness. The substitution of A178G (Ile60Val) happened to be located at amino acid 60 in the collagenous domain of porcine adiponectin which might affect the association into higher-order structures, and accordingly affect the posttranslational modifications and optimal biological activity of the multimeric forms. The identified functional polymorphism provides new evidence of adiponectin as an important candidate gene affecting fat deposition and carcass traits in pigs.

• Journal of Korea Port Economic Association
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• v.27 no.2
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• pp.1-26
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• 2011

This study was conducted to select the location of the logistics center for environment-friendly agricultural products in the Gwangyang Bay Area. AHP(Analytic Hierarchy Process) technique was used to examine location selection factors and factor hierarchy was made through a questionnaire survey and an expert interview for objective and quantitative decision. The hierarchy process of location factors of logistics center for environment-friendly agricultural products in the Gwangyang Bay Area were categorized into five factors such as natural factors, economic factors, social factors, distribution efficiency, and land use plan. Then, those factors were sub-categorized into three factors each. As a result of pair-wise comparison analysis of five categories, the weight of economic factors was the highest, and easy cargo transportation, fitness to higher-order plan, climate, land price, and limitation regulations of sub-categorized factors appeared as comparative evaluation criteria. The priority of the final candidate was decided through this process. While the weight of the Yulchon II Industrial Complex was the highest in natural and economic factors were the highest, the weight of the Gwangyang Hwanggeum Industrial Complex was the highest in social factors, distribution efficiency, and land use plan. The result of the final analysis showed that the Gwangyang Hwanggeum Industrial Complex was the most optimal location candidate for the logistics center for environment-friendly agricultural products.

• Journal of Ecology and Environment
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• v.47 no.4
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• pp.200-210
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• 2023

Background: Cambaroides similis is an endangered candidate species living in the stream of South Korea. Freshwater crayfish is known to decline rapidly not only domestically, but also internationally. Its decline is projected to be further exacerbated due to climate change. Understanding physical characteristics of the habitat is crucial for the conservation of an organism. However, comprehensive data regarding the distribution and physical habitat characteristics of C. similis are currently unavailable in South Korea. Thus, the objective of this study was to ascertain preferred ranges for water depth, current velocity, and streambed substrate of C. similis using Weibull model. Results: In this study, C. similis was found at 59 sites across 12 regions in South Korea. Its optimal water depth preferences ranged from 11.9 cm to 30.1 cm. Its current velocity preferences ranged from 9.8 cm s^-1 to 29.1 cm s^-1. Its substrate preferences ranged from -5.1 𝜱_m to -2.5 𝜱_m. Median values of central tendency were determined as follows: water depth of 21.4 cm, current velocity of 21.2 cm s^-1, and substrate of -4.1 𝜱_m. Mean values of central tendency were determined as follows: water depth of 21.8 cm, current velocity of 22.0 cm s^-1, and substrate of -4.4 𝜱_m. Mode values of central tendency were determined as follows: water depth of 21.7 cm, current velocity of 20.1 cm s^-1, and substrate of -3.7 𝜱_m. Conclusions: Based on habitat suitability analysis, physical microhabitat characteristics of C. similis within a stream were identified as Run section with coarse particle substrate, low water depth, and slow current velocity. Due to high sensitivity of these habitats to environmental changes, prioritized selection and assessment of threats should be carried out as a primary step.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.410-416
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• 2008

The gene SfXyn10, which encodes a protease-resistant xylanase, was isolated using colony PCR screening from a genomic library of a feather-degrading bacterial strain Streptomyces fradiae var. k11. The full-length gene consists of 1,437bp and encodes 479 amino acids, which includes 41 residues of a putative signal peptide at its N terminus. The amino acid sequence shares the highest similarity (80%) to the endo-1,4-${\beta}$-xylanase from Streptomyces coelicolor A3, which belongs to the glycoside hydrolase family 10. The gene fragment encoding the mature xylanase was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified to homogeneity by acetone precipitation and anion-exchange chromatography, and subsequently characterized. The optimal pH and temperature for the purified recombinant enzyme were 7.8 and $60^{\circ}C$, respectively. The enzyme showed stability over a pH range of 4.0-10.0. The kinetic values on oat spelt xylan and birchwood xylan substrates were also determined. The enzyme activity was enhanced by $Fe^{2+}$ and strongly inhibited by $Hg^{2+}$ and SDS. The enzyme also showed resistance to neutral and alkaline proteases. Therefore, these characteristics suggest that SfXyn10 could be an important candidate for protease-resistant mechanistic research and has potential applications in the food industry, cotton scouring, and improving animal nutrition.

• Journal of Ginseng Research
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• v.38 no.2
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• pp.136-145
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• 2014

Background: This study aimed to develop a biocontrol system for ginseng root rot caused by Fusarium cf. incarnatum. Methods: In total, 392 bacteria isolated from ginseng roots and various soils were screened for their antifungal activity against the fungal pathogen, and a bacterial isolate (B2-5) was selected as a promising candidate for the biocontrol because of the strong antagonistic activity of the bacterial cell suspension and culture filtrate against pathogen. Results: The bacterial isolate B2-5 displayed an enhanced inhibitory activity against the pathogen mycelial growth with a temperature increase to $25^{\circ}C$, produced no pectinase (related to root rotting) an no critical rot symptoms at low [$10^6$ colony-forming units (CFU)/mL] and high ($10^8CFU/mL$) inoculum concentrations. In pot experiments, pretreatment with the bacterial isolate in the presumed optimal time for disease control reduced disease severity significantly with a higher control efficacy at an inoculum concentration of $10^6CFU/mL$ than at $10^8CFU/mL$. The establishment and colonization ability of the bacterial isolates on the ginseng rhizosphere appeared to be higher when both the bacterial isolate and the pathogen were coinoculated than when the bacterial isolate was inoculated alone, suggesting its target-oriented biocontrol activity against the pathogen. Scanning electron microscopy showed that the pathogen hyphae were twisted and shriveled by the bacterial treatment, which may be a symptom of direct damage by antifungal substances. Conclusion: All of these results suggest that the bacterial isolate has good potential as a microbial agent for the biocontrol of the ginseng root rot caused by F. cf. incarnatum.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.139-148
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• 2015

BACKGROUND: The aim of this study was to isolate and identify algicidal bacterium that tends to kill the toxic dinoflagellate Alexandrium catenella, and to determine the algicidal activity and algicidal range of algicide. METHODS AND RESULTS: Among of algicidal bacteria isolated in this study, NH-3 isolate was the strongest algicidal activity against A. catenella. NH-3 isolate was identified on the basis of biochemical characteristics and analysis of 16S rRNA gene sequences. The NH-3 isolate showed over 99% homology with Arthrobacter oxydans, and was designated as Arthrobacter sp. NH-3. The optimal culture conditions were $25^{\circ}C$, initial pH 7.0, and 2.0% (w/v) NaCl concentration. The algicidal activity of Arthrobacter sp. NH-3 was significantly increased to maximum value in the late of logarithmic phase. Arthrobacter sp. NH-3 showed algicidal activity through indirect attack, which excreted active substance into the culture filtrate. When 10% culture filtrate of NH-3 was applied to A. catenella, 100% of algal cells were destroyed within 30 h. In addition, the algicidal activities were increased in dose and time dependent manners. The pure algicide was isolated from the ethyl acetate extract of the culture filtrate of NH-3 by using silica gel column chromatography and high performance liquid chromatography (HPLC). We investigated the algicidal activity of this algicide on the growth of harmful algal bloom (HAB) species, including A. catenella. As a result, it showed algicidal activity against several HAB species at a concentration of $100{\mu}g/mL$ and had a relatively wide host range. CONCLUSION: Taken together, our results suggest that Arthrobacter sp. NH-3 and its algicide could be a candidate for controlling of toxic and harmful algal blooms.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.199-205
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• 2000

The field control efficacy of entomopathogenic nematode, Steinernema carpocapsae Weiser, was evaluated on the beet armyworm, Spodoptera exigua (Hiibner). The insect pest has been known to be a defoliator at the aerial part of the crop and difficult to be controlled effectively with most commercial chemical insecticides due to its insecticide resistance. To overcome the susceptibility of the nematodes to desiccation when they were applied by leaf spray in field condition, we screened several commercial antidesiccants (alkyl glucoside, CMC, glycerol, Keltrol-F, Kunipia-G, and Laponite LXG) optimal for survival of the nematodes. Keltrol-F (0.1 %) was selected as a candidate supplement for field application of the nematodes. Leaf spray of the nematodes at 5,000 infective juveniles/ml of distilled water containing 0.1 % Keltrol-F resulted in 87.7% control efficacy on the 3rd instar larvae of Sp. exigua.