• Journal of Microbiology and Biotechnology
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• v.32 no.4
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• pp.464-472
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• 2022

An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45℃ and was stable at pH 3.0-6.5 and < 45℃. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the K_m and V_max values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

• Biomolecules & Therapeutics
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• v.31 no.5
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• pp.484-495
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• 2023

Idiopathic pulmonary fibrosis (IPF) can be defined as a progressive chronic pulmonary disease showing scarring in the lung parenchyma, thereby resulting in increase in mortality and decrease in the quality of life. The pathophysiologic mechanism of fibrosis in IPF is still unclear. Repetitive microinjuries to alveolar epithelium with genetical predisposition and an abnormal restorative reaction accompanied by excessive deposition of collagens are involved in the pathogenesis. Although the two FDA-approved drugs, pirfenidone and nintedanib, are under use for retarding the decline in lung function of patients suffered from IPF, they are not able to improve the survival rate or quality of life. Therefore, a novel therapeutic agent acting on the major steps of the pathogenesis of disease and/or, at least, managing the clinical symptoms of IPF should be developed for the effective regulation of this incurable disease. In the present review, we tried to find a potential of managing the clinical symptoms of IPF by natural products derived from medicinal plants used for controlling the pulmonary inflammatory diseases in traditional Asian medicine. A multitude of natural products have been reported to exert an antifibrotic effect in vitro and in vivo through acting on the epithelial-mesenchymal transition pathway, transforming growth factor (TGF)- β-induced intracellular signaling, and the deposition of extracellular matrix. However, clinical antifibrotic efficacy of these natural products on IPF have not been elucidated yet. Thus, those effects should be proven by further examinations including the randomized clinical trials, in order to develop the ideal and optimal candidate for the therapeutics of IPF.

• The Journal of the Acoustical Society of Korea
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• v.18 no.3
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• pp.3-9
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• 1999

This paper presents the study on the rejection capability based on anti-phone modeling for vocabulary independent speech recognition system. The rejection system detects and rejects out-of-vocabulary words which were not included in candidate words which are defined while the speech recognizer is made. The rejection system can be classified into two categories by their implementation methods, keyword spotting method and utterance verification method. The keyword spotting method uses an extra filler model as a candidate word as well as keyword models. The utterance verification method uses the anti-models for each phoneme for the calculation of confidence score after it has constructed the anti-models for all phonemes. We implemented an utterance verification algorithm which can be used for vocabulary independent speech recognizer. We also compared three kinds of means for the calculation of confidence score, and found out that the geometric mean had shown the best result. For the normalization of confidence score, usually Sigmoid function is used. On using it, we compared the effect of the weight constant for Sigmoid function and determined the optimal value. And we compared the effects of the size of cohort set, the results showed that the larger set gave the better results. And finally we found out optimal confidence score threshold value. In case of using the threshold value, the overall recognition rate including rejection errors was about 76%. This results are going to be adapted for stock information system based on speech recognizer which is currently provided as an experimental service by Korea Telecom.

• The Korean Journal of Applied Statistics
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• v.20 no.2
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• pp.267-280
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• 2007

Various single-valued design optimality criteria such as D-, G-, and V-optimality are used often in constructing optimal experimental designs for mixture experiments in a constrained region R where lower and upper bound constraints are imposed on the ingredients proportions. Even though they are optimal in the strict sense of particular optimality criterion used, it is known that their performance is unsatisfactory with respect to the prediction capability over a constrained region. (Vining et at., 1993; Khuri et at., 1999) We assume the quadratic polynomial model as the mixture response surface model and are interested in finding efficient designs in the constrained design space for a mixture. In this paper, we make an expanded list of candidate design points by adding interior points to the extreme vertices, edge midpoints, constrained face centroids and the overall centroid. Then, we want to propose a robust design with respect to D-optimality, G-optimality, V-optimality and distance-based U-optimality. Comparing scaled prediction variance quantile plots (SPVQP) of robust designs with that of recommended designs in Khuri et al. (1999) and Vining et al. (1993) in the well-known examples of a four-component fertilizer experiment as well as McLean and Anderson's Railroad Flare Experiment, robust designs turned out to be superior to those recommended designs.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.43 no.1 s.307
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• pp.53-66
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• 2006

This paper proposes the combined image retrieval system that gives the same relevance as exhaustive search method while its performance can be considerably improved. This system is combined with two different retrieval methods and each gives the same results that full exhaustive search method does. Both of them are two-stage method. One uses condensation of feature vectors, and the other uses binary-tree clustering. These two methods extract the candidate images that always include correct answers at the first stage, and then filter out the incorrect images at the second stage. Inasmuch as these methods use equal algorithm, they can get the same result as full exhaustive search. The first method condenses the dimension of feature vectors, and it uses these condensed feature vectors to compute similarity of query and images in database. It can be found that there is an optimal condensation ratio which minimizes the overall retrieval time. The optimal ratio is applied to first stage of this method. Binary-tree clustering method, searching with recursive 2-means clustering, classifies each cluster dynamically with the same radius. For preserving relevance, its range of query has to be compensated at first stage. After candidate clusters were selected, final results are retrieved by computing similarities again at second stage. The proposed method is combined with above two methods. Because they are not dependent on each other, combined retrieval system can make a remarkable progress in performance.

• Journal of Korean Society of Transportation
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• v.23 no.3 s.81
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• pp.35-47
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• 2005

This paper is to review a literature concerning Bus Transit Route Network Design(BTRNDP), to describe a future study direction for a systematic application for the BTRNDP. Since a bus transit uses a fixed route, schedule, stop, therefore an approach methodology is different from that of auto network design problem. An approach methodology for BTRNDP is classified by 8 categories: manual & guideline, market analysis, system analytic model. heuristic model. hybrid model. experienced-based model. simulation-based model. mathematical optimization model. In most previous BTRNDP, objective function is to minimize user and operator costs, and constraints on the total operator cost, fleet size and service frequency are common to several previous approach. Transit trip assignment mostly use multi-path trip assignment. Since the search for optimal solution from a large search space of BTRNDP made up by all possible solutions, the mixed combinatorial problem are usually NP-hard. Therefore, previous researches for the BTRNDP use a sequential design process, which is composed of several design steps as follows: the generation of a candidate route set, the route analysis and evaluation process, the selection process of a optimal route set Future study will focus on a development of detailed OD trip table based on bus stop, systematic transit route network evaluation model. updated transit trip assignment technique and advanced solution search algorithm for BTRNDP.

• Journal of the Korean institute of surface engineering
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• v.39 no.4
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• pp.179-189
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• 2006

High velocity oxygen fuel(HVOF) thermal spray coating of WC-Co powder is one of the most promising candidate for the replacement of the traditional hard chrome plating and ceramics coating because of the environmental problem of the very toxic $Cr^{6+}$ known as carcinogen and the brittleness of ceramics coating. WC-Co micron and nano powder were coated by HVOF thermal spraying method for the study of durability improvement of the high speed spindle. Coatings were planned by Taguchi program for the four spray parameters of spray distance, flow rates of hydrogen, oxygen and powder feed rate. Optimal coating process was obtained by the studies of coating properties such as porosity, surface roughness, micro hardness, and micro structure. WC-Co micron and nano powder were coated on the Inconel 718 substrate by the optimal coating process obtained in this study. The wear behaviors were studied by the sliding wear tester at room temperature and at an elevated temperature of $500^{\circ}C$ for the application to high speed spindle. Sliding wear test was carried out for four most promising hard coatings of chrome coating, ceramics coatings such as $A1_2O_3,\;Cr_2O_3$ and HVOF Co-alloy T800 for the comparison of their wear behaviors. HVOF WC-Co coating was better than other coatings showing highest micro hardness of 1400 Hv and comparable friction coefficients with others. HVOF WC-Co coating is a strong candidate for the replacement of the traditional hard chrome plating for the high speed spindle.

• Journal of Digital Convergence
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• v.19 no.1
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• pp.167-176
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• 2021

Incheon Port urgently requires designation of a free trade zone to pursue development linked with the port hinterland while promoting continuous growth of the port. This study aims to evaluate the optimal location and derive policy implications for the designation of a free trade zone and analyzed factors property divided by groups. This study used the Consistent Fuzzy Preference Relation (CFPR) analysis technique to derive a practical construction direction by quantifying and evaluating linguistic measures. As a result, the Incheon New Port hinterland showed the highest location competitiveness among the four candidate areas of Incheon New Port hinterland, Aam Logistics Complex 2, North Port hinterland, and Gyeongin Port hinterland. Among the eight evaluation factors consisting of qualitative and quantitative factors, the Incheon New Port hinterland ranked no. 1 in all the four qualitative factors and one quantitative factor and received the highest total score. Also, Group 1 presented 'possibility to attract tenant companies' as first. Group 2 was 'complex size' and Group 3 was also 'possibility to attract tenant companies'. This study has the implication for suggesting the factors and evaluation structure of Free Trade Zone. Future research requires detailed empirical studies, such as expanding the subject of study or selecting factors that reflect the interests of each group.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.1
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• pp.225-232
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• 2013

In this paper, we introduce a method that can be used to select the position of head node for context-awareness information. The validity of the head node optimal location is saving the energy in the path according to the clustering. It is important how to elect one of the relay node for energy efficiency routing. Existing LEACH algorithm to elect the head node when the node's energy probability distribution function based on the management of the head node is optional cycle. However, in this case, the distance of the relay node status information including context-awareness parameters does not reflect. These factors are not suitable for the relay node or nodes are included in the probability distribution during the head node selects occurs. In particular, to solve the problems from the LEACH-based hierarchical clustering algorithms, this study defines location with the status context information and the residual energy factor in choosing topology of the structure adjacent nodes. The proposed ECOPS (Energy Conserving Optimal path Schedule) algorithm that contextual information is contributed for head node selection in topology protocols. This proposed algorithm has the head node replacement situations from the candidate head node in the optimal path and efficient energy conservation that is the path of the member nodes. The new head node election technique show improving the entire node lifetime and network in management the network from simulation results.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.2
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• pp.135-143
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• 2012

This paper suggests the optimal blood distribution center algorithm that satisfies the minimum total transportation cost and within the allowable distribution time $T^*$. Zhang and Yang proposes shifting the location of each point that has less than the average distance of two maximum distance points from each point. But they cannot decide the correct facility location because they miscompute the shortest distance. This algorithm computes the shortest distance $l_{ij}$ from one area to another areas. Then we select the $v_i$ area to thecandidate distribution center location such that $_{max}l_{ij}{\leq}L^*$ and the $v_i$ such that $l_{ij}-L^*$ area that locates in ($v_i,v_k$) and ($v_j,v_l$) from $P_{ij}=v_i,v_k,{\cdots},v_l,v_j$ path and satisfies the $_{max}l_{ij}{\leq}L^*$ condition. Finally, we decide the candidate distribution area that has minimum transportation cost to optimal distribution area.