• Journal of Microbiology and Biotechnology
• /
• 제4권2호
• /
• pp.126-133
• /
• 1994

A Pseudomonas putida strain able to accumulate high amount of polyesters of medium-chain-length 3-hydroxyalkanoic acids ($PHA_{MCL)$) was isolated from soil in a landfill site using an enrichment technique. Culture condition of the isolated strain for polyester production in a one-step culture was optimized in a mineral-salts medium against pH and concentrations of ammonium sulfate, carbon source(e.g., octanoate), and phosphate. The optimal values for maximal cell growth and PHA accumulation were: pH; 7$\sim$8, $(NH_4)_2SO_4$; 8 mM, octanoate; 40 mM. The optimum temperature was in the range of $20\sim30^{\circ}C$, which was rather broader than in other bacteria. Cell growth was strongly inhibited by the phosphate limitation to less than 1 mM. An increase of phosphate concentration above 1 mM showed little effect on cell growth and polyester accumulation. When the strain was grown on octanoate under this optimized condition it produced 3.4 g dry biomass per liter and yielded 1.7 g PHA per liter amounting to 53 wt% of dry cells. The monomer units composing the polyester synthesized from octanoate were 3-hydroxyoctanoate (3HO), 3-hydroxycaproate (3HC), and 3-hydroxybutyrate (3HB) (85:13:2, mole ratio). Other low linear $C_3\simC_{10}$ monocarboxylic acids were also tested for polyester production.

• Advances in Computational Design
• /
• 제3권1호
• /
• pp.35-47
• /
• 2018

Design of settlement space is a complicated process while reasonable spatial layout bears great significance on the development and resource allocation of a settlement. The study proposes a weighted L-system generation algorithm based on CA (Cellular Automation) model which tags the spatial attributes of cells through changes in their state during the evolution of CA and thus identifies the spatial growth mode of a settlement. The entrance area of the Caidian Botanical and Animal Garden is used a case study for the model. A design method is proposed which starts from the internal logics of spatial generation, explores possibility of spatial rules and realizes the quantitative analysis and dynamic control of the design process. Taking a top-down approach, the design method takes into account the site information, studies the spatial generation mechanism of settlements and further presents a engine for the generation of multiple layout proposals based on different rules. A optimal solution is acquired using GA (Genetic Algorithm) which generates a settlement spatial layout carrying site information and dynamically linked to the surround environment. The study aims to propose a design method to optimize the spatial layout of the complex settlement system based on parametric generation.

• Journal of Nutrition and Health
• /
• 제35권1호
• /
• pp.115-123
• /
• 2002

This study was carried out to investigate the utilization status of internet dietary information by gender(boys: 363, girls: 366) in adolescent(middle & high school students). The results were summarized as follows. The internet using frequency of 6-7times per week had 45.0% of subjects and the using time of internet per a time was shown mainly'<2hours(68.5%)'. The main place for internet use was home(79.0%) and favorite search engine was 'Yahoo'(45.7%) and 'Daum'(19.3%). As main purpose using internet were mentioned 'social intercourse'(45.0%) and data search'(24.8%). The organization that offer to reliable internet information was educational institution'(49.4%). The problems in using information site were 'poor information'(26.4%), 'slow connection speed'(22.6%), and 'don't arouse interest'(18.8%). The search experience about dietary information had only 27.9% of subjects and search purpose was 'for homework'(33.3%) and 'for health'(32.0). The satisfaction degree of dietary information was not high. The connection motive to dietary information was mainly 'by site navigation casualty'(55.7%). Only 7.7% of subjects had experience of nutrition counseling using internet, and the motive of nutrition counseling was also 'by site navigation causally'(55.8%). The purpose of counseling was 'for diet'(41.5%) and 'for health problem'(30.2%), and the satisfaction degree of counseling result was very low. As the ask of improvement for counseling site were pointed out 'poor in answer content'(44.8%) and 'lazy answer'(31.0%). The subjects wanted to get the dietary information about 'growth in status'(41.4%), 'diet related skin beauty'(14.6%), the update period less 1 month, and the way of 'free board'(32.3%), 'game'(21.1%) and 'animation'(19.3%) as offer tool. The results of this study showed that although the internet using percent and frequency of subjects was high, they used dietary information very seldom and they are dissatisfied with internet nutritional information. Therefore, the information donor should consider which dietary information was needed and what is the optimal tool for adolescent.

• The Korean Journal of Physiology and Pharmacology
• /
• 제18권4호
• /
• pp.289-296
• /
• 2014

Human adipose-tissue-derived stromal cells (hADSCs) are abundant in adipose tissue and can differentiate into multi-lineage cell types, including adipocytes, osteoblasts, and chondrocytes. In order to define the optimal harvest site of adipose tissue harvest site, we solated hADSCs from different subcutaneous sites (upper abdomen, lower abdomen, and thigh) and compared their proliferation and potential to differentiate into adipocytes and osteoblasts. In addition, this study examined the effect of phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, on proliferation and differentiation of hADSCs to adipocytes or osteoblasts. hADSCs isolated from different subcutaneous depots have a similar growth rate. Fluorescence-activated cell sorting (FACS) analysis showed that the expression levels of CD73 and CD90 were similar between hADSCs from abdomen and thigh regions. However, the expression of CD105 was lower in hADSCs from the thigh than in those from the abdomen. Although the adipogenic differentiation potential of hADSCs from both tissue regions was similar, the osteogenic differentiation potential of hADSCs from the thigh was greater than that of hADSCs from the abdomen. Phorbol 12-myristate 13-acetate (PMA) treatment increased osteogenic differentiation and suppressed adipogenic differentiation of all hADSCs without affecting their growth rate and the treatment of Go6983, a general inhibitor of protein kinase C (PKC) blocked the PMA effect. These findings indicate that the thigh region might be a suitable source of hADSCs for bone regeneration and that the PKC signaling pathway may be involved in the adipogenic and osteogenic differentiation of hADSCs.

• 한국환경과학회지
• /
• 제27권11호
• /
• pp.1059-1072
• /
• 2018

Bioremediation has been recognized as a suitable alternative to conventional methods of removing contaminants, and it uses fungi, bacteria and microalgae. In contrast to other organisms, microalgae are unique in that they have the ability to perform photosynthesis like plants and to utilize organic/inorganic carbon substrates, in a process called phytoremediation. Microalgae can populate a reaction site rapidly and enhance the bioremediation efficiency. In this study, Chlorella vulgaris was used to evaluate the removal potentials of the nutrients (N and P) and heavy metals (Cu and Zn) from swine wastewater. The optimum growth conditions for Chlorella vulgaris and the removal potentials of N, P, Cu, and Zn from synthetic wastewater using Chlorella vulgaris were investigated. Based on the results, the applicability of this microalga to on-site wastewater treatment was examined. Optimal growth conditions for Chlorella vulgaris were established to be $28^{\circ}C$, a pH of 7, and light and dark cycles of 14:10 h. As the concentrations of the nutrients were increased, the efficiencies of N and P removal efficiencies by Chlorella vulgaris were decreased in the single and binary mixed treatments of the nutrients, respectively. Further, the efficiencies of Cu and Zn removal also decreased as the heavy metals concentrations added were increased, both in the single and binary mixed treatments. In addition, the efficiency of Cu removal was higher than that of Zn removal. Our results indicate that Chlorella vulgaris could be used in treatment plants for the removal of nutrients and heavy metals from swine wastewater.

• 한국인터넷방송통신학회논문지
• /
• 제18권4호
• /
• pp.155-159
• /
• 2018

본 논문은 수산물의 생육환경을 위하여 현장관리자가 원격으로 모니터링할 수 있는 시스템을 설계 제작하였다. 활수산물의 유통과정에서 발생할 수 있는 문제점은 시스템과 환경 모니터링을 모듈화시켜 해결하였다. 모의실험을 위하여 컨테이너 내부에 산소공급장치와 슬러지 처리장치, 모니터링 시스템을 설계하고 유통환경을 구축하여 실시간으로 모니터링 할 수 있도록 하였다. 또한 데이터베이스 및 통신환경으로 모니터링 시스템을 구축하였으며 센서를 통하여 생육에 맞는 최적의 환경을 마련하였다. 그 결과 수산물의 저장 및 유통을 위한 환경 모니터링을 할 수 있었으며 적합한 활수산물 관리시스템을 구축할 수 있었다.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2005년도 학술발표회 논문집
• /
• pp.260-264
• /
• 2005

Effective use of water resources has become a social problem because the deficit of water comes from population growth and Industrial development. Therefore, the conjunctive operation of surface water and groundwater will become an alternative. Groundwater has many advantages for the evaporation and effect of rainfall compared with surface water. Although the available amount of groundwater is small, groundwater dam can be used complementarily because of the sustainable supply of water. A calculating technique of the optimal amount of water resources by the groundwater dam was developed. A pilot site was selected to assess the optial amount of groungwater for the designed groungwater dam. If the developed technique is more refined by the measured data, the groundwater dam will become a good alternative to develope the water resources in the water deficit area.

• 한국식품영양과학회:학술대회논문집
• /
• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
• /
• pp.152-160
• /
• 2001

The ability of microbial strains to confer health benefits to human and animal hosts is not a feature of many organisms. Lactic acid bacteria are the most commonly used bacteria applied as probiotics and there is now strong evidence that certain strains confer tangible benefits to the host. In terms of preventing infection, the ability of probiotic lactobacilli to colonize the tissue site, even temporarily, and inhibit growth and adhesion of pathogens, has been documented. Using molecular tools, such as RAPD and DGGE, probiotic organisms can be tracked through the intestine and in the vagina, and their impact on the flora assessed. Arguably, strains L. rhamnosus GG and GR-l are the most studied probiotic strains in terms of human application to the gut (GG and GR-l) and vagina (GR-1). Combined with L. fermentum RC-14, GR-l provides a two-pronged therapeutic for the intestine and urogenital tract. Care in manufacturing and distribution is essential to ensure that optimal doses of probiotics are accessible to consumers and patients.

• KEPCO Journal on Electric Power and Energy
• /
• 제8권2호
• /
• pp.151-157
• /
• 2022

The transition to renewable energy, especially the expansion of photovoltaic (PV) generation, has become a global megatrend that can no longer be reserved. However, since the site for PV is limited, it is necessary to use the land efficiently. As an alternative, the concept of utility scale agricultural PV(UAPV) is a technology that continues farming in the lower part and installs PV in the upper part of farmland to efficiently use the land. Therefore, for UAPV, the growth of crops in the lower part and the optimal operation of PV in the upper part are important. In this, we analyze the characteristics of the upper PV generation system in the UAPV based on the empirical results under various conditions, and propose future research directions of the UAPV.

• 대한지역사회영양학회지
• /
• 제8권1호
• /
• pp.15-25
• /
• 2003

This study was carried out to investigate the utilization status of internet and dietary information by gender (boys : 442, girls : 461) in school children (total 903). The results were summarized as follows. The most of children used internet regularly (98.1%) and major purpose of using were mentioned as 'game (39.0%)' and 'social intercourse (49.5%)'. The duration of internet use was '< 2hours (80.9%)' They used internet mainly at 'home (88.8%)', and favorite search engines were 'Yahoo (54.2%)' and 'Daum (31.1%)'. The searching experience on dietary information was from only 35.6% of subjects mainly 'for homework (39.6%)' and 'for health (36.9%)'. The satisfaction degree of searched information was 'high (79.5%)'. Dissatisfactory reasons of internet site for dietary information were pointed out to be 'bring little interest (28.9)', 'difficult contents (19.2%)', and 'poor Information (18.2%)'. Only fifteen % of subjects had experience of nutrition counseling using internet, and purpose of counseling was mainly 'for homework (51.4%)' and 'for health problem (24.3%)'. The problems for nutritional counseling site were pointed out to be 'difficult answer content (31.7%)', 'insincere answer (28.6%)'and 'poor answer content (25.4%)'. They acquire information of nutrition and health management mainly through 'internet (43.7%)'. 'Growth and nutrition (28.3%)', 'improvement in studying ability (13.8%)', 'right weight control (13.3%)' and 'cooking (12.8%)'were most frequently asked information, They had a preference for 'game (40.5%)', 'animation (29.9%)' and 'quiz (18.1%)'as loaming method tools. The favorite site color was 'green (51.3%)'The results of this study showed that although the internet use was very high, they used internet to search dietary information very seldom. Therefore, the information donor should find out what is the optimal tool, what kind of dietary information was needed for school children.