• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.162.6-162
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• 2001

Confocal scanning microscopy (CSM) is an important instrument in a wide variety of imaging applications because of its ability to provide three-dimensional images of thick, volume specimens. The mechanism for two-dimensional beam scanning and optical sectioning has an important roe in CSM as the three-dimensional profiler. This optical sectioning property arises from the use of a point detector, which serves to attenuate the signals from out-of-focus. The intensity profile for the open loop scanning should be matched with its response for the standard. The non-linearity can be minimized with the optical sectioning or the optical probe of the closed loop control. This paper shows the mathematical expression of the light such as the extinction curve in the optical fields of system using AO deflector, the axial/lateral response experimentally when the error sources change, and the methods of optical sectioning. Thorough design of optical sectioner is crucial to the success of CSM in the field ...

• 한국가시화정보학회지
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• 제3권1호
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• pp.3-19
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• 2005

Novel optical techniques are presented for three-dimensional tracking of nanoparticles; Optical Serial Sectioning Microscopy (OSSM) and Ratiometric Total Internal Reflection Fluorescent Microscopy (R-TIRFM). OSSM measures optically diffracted particle images, the so-called Point Spread Function (PSF), and dotermines the defocusing or line-of-sight location of the imaged particle measured from the focal plane. The line-of-sight Brownian motion detection using the OSSM technique is proposed in lieu of the more cumbersome two-dimensional Brownian motion tracking on the imaging plane as a potentially more effective tool to nonintrusively map the temperature fields for nanoparticle suspension fluids. On the other hand, R-TIRFM is presented to experimentally examine the classic theory on the near-wall hindered Brownian diffusive motion. An evanescent wave field from the total internal reflection of a 488-nm bandwidth of an argon-ion laser is used to provide a thin illumination field of an order of a few hundred nanometers from the wall. The experimental results show good agreement with the lateral hindrance theory, but show discrepancies from the normal hindrance theory. It is conjectured that the discrepancies can be attributed to the additional hindering effects, including electrostatic and electro-osmotic interactions between the negatively charged tracer particles and the glass surface.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 춘계학술대회 논문집
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• pp.187-190
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• 2002

Confocal microscopy is very popular technology in bio-medical inspection due to its ability to reject background signals and to measure very thin slide of thick specimens, which is called optical sectioning. But intensity of detected signal in fluorescence type confocal microscopy is so small that only 0.2% of emitted fluorescence light can be detected in the best case. In this paper, we proposed the reflecting optical system to improve the detection intensity and designed the optical system by optimal design method. At the end of the paper, we analyzed the characteristics of the proposed reflecting optical system.

• 한국식품위생안전성학회지
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• 제16권3호
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• pp.232-240
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• 2001

시장에서 수집한 갈변병에 감염된 느타리버섯으로부터 125 균주를 분리하였고 그 중 45 균주는 병원성 균주로 조사되었다. WLFO 6 strains, WLFO 6 strains으로 나타났고, WLRO의 몇 균주는 병원성 검정 실험에서 약한 병원성을 나타내기도 했다. WLFO 6균주는 모두 느타리버섯의 갈변병 주원인균으로 알려진 P. tolaasii로 미생물 신속 동성을 (MIDI, gas chromatograph-microbial identification system)에 의해 동정되었고 WLRO는 P. gingeri, P. fluorescens biotype A and type C. 로 동정되었다. 그 외의 선발된 Pseudomonas spp.는 P. gingeri, P. agarici, P. fluorescens biotype B, P. chloroaphis, non-pathogenic P. tolaasii, P. putida biotype A, B 등으로 동정이 되었다. 분리된 병원성 세균의 세포배양 여과액으로 버섯에서의 갈변과 조직 함몰을 실험하였다. 병원성 검정에서 약한 병원성을 보인 분리균들은 갈변 또는 조직함몰이 나타나지 않았으나 강한 병원성을 나타냈던 균들은 두 현상이 모두 나타났다. P. tolaasii에 의해서 생성되는 세포외독소인 tolaasin의 활성을 조사하기 위하여 600 nm에서 용혈 활성을 측정하였다. P. tolaasii로 동정된 6 균주는 0.8∼0.9, 약한 병원성의 WLROs는 0.9∼1.0 그리고 Pseudomonas spp.는 10∼1.2로 나타났다. 공초점 현미경 기술을 이용하여 신선한 버섯에서의 조직을 optical sectioning image와 vertical sectioning image로 관찰하였고 또한 P. toiaasii에 의하여 오염된 조직부위의 영상을 회득하였다.

• 한국광학회지
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• 제20권6호
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• pp.361-365
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• 2009

본 연구에서는 패턴 빔을 이용하여 표면이 거친 물체의 단차를 비접촉 방식으로 측정 하는 방법에 대하여 연구하였다. 본 연구에 사용된 방법은 장치가 매우 간단하고 스페클 노이지가 영상에 미치는 영향이 작아 재현성 면에서 매우 우수하였다. 특히 기준면에 문양이 없는 경우에는 기존의 자동 초점 측정 장치로는 측정하기 어려우나, 본 연구 방법에 의해 측정이 가능함을 보였다. 그리고 측정 시간이 매우 짧고 재현성이 좋으며 장치가 간단하여 산업 현장에서 응용하기 적절한 방법이다.

• Current Optics and Photonics
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• 제8권1호
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• pp.1-15
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• 2024

This paper presents a brief introduction to self-interference incoherent digital holography (SIDH). Holography conducted under incoherent light conditions has various advantages over digital holography performed with a conventional coherent light source. We categorize the methods for SIDH, which divides the incident light into two waves and modulates them differently. We also explore various optical concepts and techniques for the implementation and advancement of SIDH. This review presents the system design, performance analysis, and improvement of SIDH, as well as recent applications of SIDH, including optical sectioning and deep-learning-based SIDH.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1995년도 추계학술대회
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• pp.257-261
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• 1995

Confocal laser scanning microscopy (CLSM)는 기존의 coherent or incoherent microscopic imaging 보다 횡축 방향 (lateral direction)으로 고해상도를 가지며, 층과 층 사이를 구분하는 광축 방향 (axial direction)의 optical sectioning에 의해 샘플의 3D 구조를 고해상도로 영상화함으로써 3D 구조 및 생체 기능 분석을 가능하게 해 준다. 본 논문에서는 CLSM에 의한 3D 영상화 원리와 촛점면 부근에서 얻어지는 광세기 분포, 얻어진 2D slice 영상의 시각화 및 응용에 대해 논의된다.

• Journal of the Optical Society of Korea
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• 제1권2호
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• pp.90-93
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• 1997

The shallow depth of focus in conventional light microscopy hinders the observation of the whole image when the object is thicker than the depth of field. Most of the existing techniques measured the object distance, which is not necessarily the actual distance of each pixel in the image. We implemented a means of determining the "best focus" of each pixel and located the height of object points by sectioning at different sample heights. Combining the height information and its gray values together, we obtained an image where the blur from the finite depth of focus is eliminated. Limitations of the technique are discussed together with composed images.ed images.

• Current Optics and Photonics
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• 제2권6호
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• pp.595-605
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• 2018

The two-photon process of microscopy provides good spatial resolution and optical sectioning ability when observing quasi-static endogenous fluorescent tissue within an in vivo animal model skin. In order to extend the use of such systems, we developed a two-photon laser scanning microscopy system capable of also capturing $512{\times}512$ pixel images at 90 frames per second. This was made possible by incorporating a 72 facet polygon mirror which was mounted on a 55 kRPM motor to enhance the fast-scan axis speed in the horizontal direction. Using the enhanced temporal resolution of our high-speed two-photon laser scanning microscope, we show that rapid processes, such as fluorescently labeled erythrocytes moving in mouse blood flow at up to 1.2 mm/s, can be achieved.

• 대한의생명과학회지
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• 제18권2호
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• pp.160-164
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• 2012

The baculovirus/insect cell expression system is of great value for the large-scale production of normal and mutant mammalian passive glucose-transport proteins heterologously for structural and functional studies. In most mammalian cells that express HepG2, this transporter isoform is predominantly located at the cell surface. However, it had been reported that heterologous expression of other membrane proteins using the baculovirus system induced highly vacuolated cytoplasmic membranes. Therefore, how a cell responds to the synthesis of large amounts of a glycoprotein could be an interesting area for investigation. In order to examine the subcellular location of the human HepG2 transport proteins when expressed in insect cells, immunofluorescence studies were carried out. Insect cells were infected with the recombinant baculovirus AcNPVHIS-GT or with wild-type virus at a MOI of 5, or were not exposed to viral infection. A high level of fluorescence displayed in cells infected with the recombinant virus indicated that transporters are expressed abundantly and present on the surface of infected Sf21 cells. The evidence for the specificity of the immunostaining was strengthened by the negative results shown in the negative controls. Distribution of the transporter protein expressed in insect cells was further revealed by making a series of optical sections through an AcNPVHIS-GT-infected cell using a confocal microscope, which permits optical sectioning of cell sample. These sections displayed intense cytoplasmic immunofluorecence surrounding the region occupied by the enlarged nucleus, indicating that the expressed protein was present not only at the cell surface but also throughout the cytoplasmic membranous structures.