• Fisheries and Aquatic Sciences
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• v.27 no.9
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• pp.539-551
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• 2024

The enormous diversity of molluscs has provided humans with food, colours, medicines and shells, among other resources. Molluscs have long been utilized in traditional medicine in several countries and they are a valuable source of medical supplies for many diverse communities worldwide. The purpose of this study was to identify and assess Cipangopaludina lecythis bioactive compounds in order to determine the nature of the primary ingredient that gives its medicinal properties. C. lecythis flesh and shell extracts using polar and lipophilic solvents were analyzed using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS). This study provides the first chemical assessment of flesh and shell-opercula of C. lecythis. Chemical analysis of flesh and shell-opercula of C. lecythis clearly showed the presence of major compounds such as chitin, allantoin, linoleic acid, dihydrotachysterol, cyclotrisiloxane hexamethyl and 6-gingerol besides other minor compounds with bioactive properties of medicinal significance. Overall, this research provides good evidence that C. lecythis produce secondary metabolites with a variety of intriguing pharmacological characteristics. They have also long been a part of traditional medicine in many human cultural groups. Its usage by traditional practitioners to treat a range of human diseases is justified by the presence of numerous medicinally significant bioactive chemicals. However, more research on the bioactive compounds found in snails is necessary to standardize the extraction techniques for their detection, quantification and formulations, to validate their in vivo efficacy, and confirm their safety.

• The Korean Journal of Zoology
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• v.19 no.4
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• pp.171-178
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• 1976

A new species of Cobitid fish, Cobitis longicorpus is described, based upon many specimens from several tributaries of the Seomjin River situated in the south western part in Korea. Cobitis longicorpus was previously reported as the crossband type of C. taenia by all ichthyologists in Korea. This is distributed in the Seomjin River and characterized by the distinctive cross-band color pattern, the black spots at the back of its opercula, and the structure of lamina circularis in male.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.209-213
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• 1976

Spinous loaches widely distributed in Korea, are of many color pattern types, but interestingly the crossband B and C-types of these coaches are collected only in the two rivers, the Yeongsan River and the Seomjin River, flowing through the south-western part of Korea. These two types have not only different color pattern, but also different geographical distribution: the B-type is found in the Yeongsan River and the C-typs, in the Seomjin River. But the both types have four common distinctive features, which easily distinguish them from other already known species of this genus: the B and C-types have the similar total length distribution, the same structure of lamina circularis and scales, and the black spots at the back of their opercula. These peculiarities may be explained by studing the history of these two rivers. The author considers that they are once rivers of the same origin located around the Noryeong and the Sobaeg Mountains, and then were eventually separated from each other as the water level or the crust of Korea peninsula changed. Thus, these two types, B and C, of spinous loaches should have been the same species living in the same river, but in the course of time, these two types have taken different color patterns since the separation of two rivers thus making them impossible to cross between these two populations.

• Journal of fish pathology
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• v.34 no.2
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• pp.255-259
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• 2021

This is the first report describing acute mass mortality occurred in juvenile olive flounder (Paralichthys olivaceus) caused by gas bubble disease (GBD). A total of 610 fish (average weight = 35 g), which were more than half of the fish acclimated at 17℃ in an aquarium, were killed within two days of acclimation. The dead and moribund fish showed excessively opened opercula and mouths, and occasionally, severe exophthalmia. Through microscopic observation, numerous gas emboli were found in the gills of the dead and live fish, while the fish were not infected with any microbial pathogens. The dissolved oxygen (DO) saturation level of the rearing water and seawater nearby the facility reached 145% and 286%, respectively, whereas other water quality parameters (such as salinity, pH, and chemical oxygen demand) were normal. The extreme saturation rate of seawater in the shore nearby seemed to be due to an enormous algal bloom that occurred there. Through molecular identification based on 18S rDNA sequences, the most dominant algal species was most closely related to Ulva californica (99.87% sequence identity) followed by U. prolifera, U. linza, and U. curvata (99.81%). Therefore, it can be concluded that supersaturated seawater due to mass algal bloom caused gas bubble disease in the olive flounder, leading to mass mortality. After technical adjustment, such as increased aeration, lowered water circulation rate, and inlet water filtration using micro-pore carbon filters, the DO level became normal, no further mortality occurred and the status of the fish was stabilized.

• Journal of fish pathology
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• v.14 no.2
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• pp.71-80
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• 2001

The purpose of this study was to investigate the microbiological characteristics and the distributions of the bacteria causing streptococcicosis occurred in marine fish farm, Korea. Many kinds of cultures fishes suffered from the disease accompanied with typical symptoms, including darkening of the skin, exophthalmia, petechiae inside of the opercula and distended abdomen. The isolates from the diseased fishes were compared with Lactococcus garvieae by biochmical, biophysical and serological methods and polymerase chain reaction(PCR) assay. We isolated 35 strains of the geuns Streptococcus from the diseased olive flounder, Paralichthys olivaceus, yellow tail, Seriola quinqueradiata and Korean rockfish, Sebastes schlegeli. 15 strains out of the isolates were identified to L. garvieae and the others were not because of their different biochemical and biophysical charateristics. Seven strains of the isolates were agglutinated by rabbit serum raised against L. garvieae $KG^+$ phenotypic cells(ATCC49156)as a reference strain. Twenty-one strains of the isolates identified to L. garvieae since they were formed the expected band through performing PCR assay using specific primers, pLG-1(5'-CATAACAATGAGATCGC-3') and pLG-2(5'-GCACCCCGCGGTTG-3'). In the present study, it showed that L. garvieae was a dominant strain causing streptococcicosis in the tested area due to occurrence of 21 strains as L. garvieae out of all the isolates, 9 atrains as Streptococcus sp. and 5 strains as Enterococcus sp.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.4
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• pp.665-670
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• 2004

The mandibular buccal frenum becomes a problem when its attachment is too close to the marginal gingival. Heavy buccal frenum mucogingivally results in insufficient attached gingiva, inadequate vestibular depth and also difficulty in eruption of mandibular premolar. The aim of this study is to demonstrate the effectiveness of apically positioned partial-thickness flap as adequate method in order to remove heavy buccal frenum and expose impacted teeth. The author presents the case of a 12-year-old boy. He had problems that was prolonged eruption of #34, #35 due to high pull buccal frenum. After space regaining for normal eruption of #34, #35, we performed apically positioned partial-thickness flap on #34, 35 area. As a results, we confirmed that eruption of #34, #35 has been processed normally And vestibular depth, position of buccal frenum and width of attached gingival was within a normal range. Decrease in muscle pull, adequate width of attached gingiva and increased vestibular depth can be expected from this treatment.

• Journal of fish pathology
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• v.25 no.3
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• pp.151-158
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• 2012

Diseased eel (Anguilla bicolor) displayed severe hemorrhages in the gills, and congestion and swelling in the liver. During the epizootic, the water temperature was $28^{\circ}C$ and the morality rates were about 5%. No parasites were found on the gills and skin. Bacteria were not cultured from any internal organs using TSA or SS agar at $28^{\circ}C$ for 48 hrs. Histopathologically, the gills showed epithelial hyperplasia in the base of secondary gill lamellae and hemorrhages in the capillaries. Some cells in the proliferated interlamellar epithelia exhibited marginal hyperchromatosis. And severe vacuolated changes in the parenchymal cells and congestion in the central veins were observed in the liver. The specific amplicon (396 bp) was detected from gills and opercula of affected eel PCR using Anguillid herpesvirus-1 (AngHV-1) -specific primer sets HVAPOLVPSD (5-'GTG TCG GGC TTT GTG GTG C-3') and HVAPOLOOSN (5'-CAT GCC GGG AGT CTT TTT GAT-3'). Sequencing analysis of the amplicon demonstrated that this gene was 99% homologous to the AngHV-1 sequence deposited in GenBank. This is the first report of AngHV-1 outbreak in the farmed shortfin eels (A. bicolor) in Korea. When diseased fish were maintained for 10 days at water temperatures of $32^{\circ}C$ and $35^{\circ}C$, the cumulative mortalities were 100% and 10%, respectively. Even though the AngHV-1 genome in the gills from the eel kept at $35^{\circ}C$ was detected using PCR, the structure of gill filaments was similar with that of normal fish. Increasing the water temperature to $35^{\circ}C$ was an effective way to diminish the mortality of AngHV-1 affected eel.