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Occurrence and antibiotic resistance of Enterococcus spp. from retail fresh-cut products in Korea (국내 신선 편이식품으로부터 분리한 Enterococcus의 항생제 저항성)

  • Kim, Hyun Jung;Kim, Seung Min
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.581-586
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    • 2018
  • Enterococcus spp. have been considered major indicator organisms for antibiotic resistance due to their ability to easily acquire and to harbor antibiotic resistance. In this study, Enterococcus spp. were isolated from 174 retail fresh-cut products (fresh vegetable salads, microgreens, and sprouts) in Korea. Among the 20 Enterococcus isolates obtained, 18 (90.0%) were Enterococcus faecalis and 2 (10.0%) were Enterococcus faecium. The patterns of antibiotic resistance against nine antimicrobials were analyzed. Most of the isolates (85.0%) were resistant to quinupristin/dalfopristin, 40.0% and 50.0% of the isolates showed intermediate resistance to two critically important antimicrobials for human medicine, ciprofloxacin and erythromycin, respectively. Vancomycin-resistant enterococci were not detected in this study. Given the importance of antimicrobial resistance of enterococci in food safety as well as in public health, our results regarding the occurrence (level of contamination) and antimicrobial resistance of Enterococcus spp. could provide useful information that aids the risk analysis of antibiotic resistance.

Effect on Acute reflux Esophagitis by Evodiae Fructus Aquous Extract (오수유(吳茱萸) 물 추출물이 급성역류성 식도염에 미치는 효과)

  • Kim, Dae-Jun;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.27 no.1
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    • pp.51-58
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    • 2012
  • Objectives : This study was performed to investigate effect of evodiae fructus on acute reflux esophigitis rat induced by pylorus and forestomach ligation operation. Methods : Twenty-four laboratory rats were divided four groups and each group had six rats ; normal intact group, acute reflux esophagitis (RE) control group, two experiment RE group treated extract of evodiae fructus 600 mg/kg (EEF600) and 300 mg/kg (EEF300). All rats was fasted for 18 hr but free water, we induced RE by pylorus and forestomach ligation operation. Intact group and RE control group rats were orally administered a distilled water and two experiment groups were orally administed with EEF 600 mg/5ml/kg and 300 mg/5ml/kg. One hour after, rats were anesthetized, intact group was cut the abdomen open and sutured with 2.0 silk thread. RE control group and EEF group were cut the abdomen open, ligated pyloric canal and forestomach with 2.0 silk thread and sutured. Six hour after the operation, rats were sacrified, collected bloods in the abdominal vein, disectted a esophagus and stomach. The stomach was washed a 1 ml PBS and the esophagus was cut longitudinally and pictured a innter mucosa area to research damages in esophagus. Results : The esophagic tissue damage percentage of reflux esophagitis rat was increased compared to that of normal intact group. But esophagic damage percentage of EEF 600 were significantly decreased compared to that of RE control group. But there was no difference on gastric juice pH between control RE, alpha-tocopherol administration rat group and EEF administration rat group. In esophagus of RE control rat, gastric damage occurred severely and injury percentage of mucosa were increased, but EEF 600 mucous inflammatory damage percentage was significantly compared to that of RE control group. Proinflammatory cytokines such as TNF-alpha, IL-1beta and IL-6 in serum on RE control group were markedly grew than those of intact rat, those of vechicle group treated with EEF 600 and EEF 300 were remarkably decreased compared to production of proinflammatory cytokine of RE control group. In microscopic observation, intact group rat had no hyperemia, mucous injury and exclusion, ulcer and edema. But it could showed mucosa damages, submucosa edema and ulcer in RE control. However, administration of EEF 600 and EEF 300 made esophagus have less inflammation and injury by gastric acid. Conclusions : The results suggest that antiinflammatory Effect of EEF could attenuate the severity of reflux esophagitis and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease.

A Low-pass Filter with Wide-stop Band Using Radial-shaped Open Stub (광대역의 저지대역을 갖는 부채꼴 형태의 개방형 스터브를 이용한 저역통과 여파기)

  • Yoon, Ki-cheol;Kim, Seong-cheol
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.7
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    • pp.1237-1242
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    • 2016
  • In this paper, the low pass filter with harmonics suppression and rejection using parallel coupled-line and radial type open stub is presented. And the resultant characteristic of the proposed low pass filter is that the harmonics are suppressed and rejected in wide bandwidth due to the parallel coupled-line structure. The open stub of a low pass filter is constructed in radial type which can be used to adjust the size of filter. The size of the proposed low pass filter is $6.98{\times}7.60mm2$ and the cut off frequency is 2.45 GHz. And the filter is economical in unit cost and can be constructed easily and has the merit of mass product because the filter is composed of distributed element. Also, the widely rejected harmonics is 128 % in the bandwidth and the insertion and return losses of the low pass filter are 1.07 dB and 19.5 dB, respectively.

The Method of Real-Time Location Information Service by using GPS and OpenAPI for Cost-cutting (비용 절감을 위한 GPS와 OpenAPI을 이용한 실시간 위치정보 서비스 방안)

  • Youn, Jae-Hong;Kim, Eun-Seok;Hur, Gi-Taek
    • Proceedings of the Korea Contents Association Conference
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    • 2008.05a
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    • pp.902-905
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    • 2008
  • With development of internet, location-based service (LBS) is getting more interest in wire and wireless network, and many studies are in progress to provide various services such as GIS and GPS technique related to this. Today positioning and information providing service is accomplished by mobile communication companies' chasing the cell or building unique GIS system, but there is a lot of building-cost that must be endured to define boundary of collecting precise location information from chasing Cell and provide unique location information service. In this paper, it desires to make the database of location information with using GPS, relatively precise satellite positioning system, to cut the cost of building location information service, and to present service models such as children's positioning service that is to reduce the cost of building GIS by using Web Open API and provide location information of discrete object in real-time.

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Effect of Residual Stress on Femoral Arterial Stress-Strain Behavior

  • Chandran, K.B.;Mun, J.H.;Chen, J.S.;Nagaraj, A.;McPherson, D.D.
    • Journal of Mechanical Science and Technology
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    • v.15 no.7
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    • pp.965-973
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    • 2001
  • It is well established that arteries are subjected to residual stress. Due to the effect of residual stress, the arteries open to a horse-shoe shape when a longitudinal cut is made on an excised arterial segment. Previously, the residual stress has been quantified by the opening angle of the horse-shoe shape. We have employed a finite element analysis of the open arterial segment to restore the same to the original cylindrical shape and computed the circumferential strain as well as the stress distribution in the wall. In this study, the stress distribution in the femoral arteries of miniswine was computed with and without the residual stress for a range of transmural pressures. Our analysis showed that the residual stress has the effect of redistribution of the circumferential stresses between the intima and the adventitia under physiological loading. The redistribution of the stress with the inclusion of residual stress may be important in the studies on effect of wall stresses on the endothelial and vascular smooth muscle cells.

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Cloning and Sequence Analysis of Two Catechol-degrading Gene Clusters from a Phenol-utilizing Bacterium Pseudomonas putida SM25

  • Jung, Young-Hee;Ka, Jong-Ok;Cheon, Choong-Ⅰll;Lee, Myeong-Sok;Song, Eun-Sook;Daeho Cho;Park, Sang-Ho;Ha, Kwon-Soo;Park, Young-Mok
    • Journal of Microbiology
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    • v.41 no.2
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    • pp.102-108
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    • 2003
  • A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the veetor pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed activesite region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, the could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RBI.

Numerical Simulations of Added Resistance and Motions of KCS in Regular Head Waves (선수 규칙파 중 KCS의 부가저항 및 운동성능 수치해석)

  • Seo, Seonguk;Park, Sunho
    • Journal of the Society of Naval Architects of Korea
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    • v.54 no.2
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    • pp.132-142
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    • 2017
  • As the International Maritime Organization (IMO) recently introduced the Energy Efficiency Design Index (EEDI) for new ships building and the Energy Efficiency Operational Indicator (EEOI) for ship operation, thus an accurate estimation of added resistance of ships advancing in waves has become necessary. In the present study, OpenFOAM, computational fluid dynamics libraries of which source codes are opened to the public, was used to calculate the added resistance and motions of the KCS. Unstructured grid using a hanging-node and cut-cell method was used to generate dense grid around a wave and KCS. A dynamic deformation mesh method was used to consider the motions of the KCS. Five wavelengths from a short wavelength (${\lambda}/LPP=0.65$) to a long wavelength (${\lambda}/LPP=1.95$) were considered. The added resistance and the heave & pitch motions calculated for various waves were compared with the results of model experiments.

A Location-Routing Problem for Logistics Network Integrating Forward and Reverse Flow (역물류를 고려한 통합물류망에서의 입지:경로문제)

  • Na, Ho-Young;Lee, Sang-Heon
    • IE interfaces
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    • v.22 no.2
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    • pp.153-164
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    • 2009
  • An effective management for reverse flows of products such as reuse, repair and disposal, has become an important issue for every aspect of business. In this paper, we study the Location-Routing Problem (LRP) in the multi-stage closed-loop supply chain network. The closed-loop supply chain in this study integrated both forward and reverse flows. In forward flow, a factory, Distribution Center (DC) and retailer are considered as usual. Additionally in reverse flow, we consider the Central Returns collection Center (CRC) and disposal facility. We propose a mixed integer programming model for the design of closed-loop supply chain integrating both forward and reverse flows. Since the LRP belongs to an NP-hard problem, we suggest a heuristic algorithm based on genetic algorithm. For some test problems, we found the optimal locations and routes by changing the numbers of retailers and facility candidates. Furthermore, we compare the efficiencies between open-loop and closed-loop supply chain networks. The results show that the closed-loop design is better than the open one in respect to the total routing distance and cost. This phenomenon enlarges the cut down effect on cost as an experimental space become larger.

Effect of Cycloheximide and Holding Solution on Vase Life of Cut 'Blue Magic' Iris Flowers according to the Flower Development and Opening Stages (Cycloheximide 및 보존용액 처리가 개화단계에 따른 절화 아이리스 '블루매직'의 수명에 미치는 영향)

  • Kim, Young-A;Lee, Jong-Suk
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.790-795
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    • 2010
  • This experiment was conducted to clarify the effects of cycloheximide and holding solution on vase life of cut 'Blue Magic' iris. The vase life of iris flowers held in 3% sucrose (S) + $100mg{\cdot}L^{-1}$ hydroxy quinoline sulfate (HQS) + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ Benzylaminopurine (BA), 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ cycloheximide (CHI), or 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI were much longer than those held in distilled water. Squeeze stem phenomenon that showed at a holding solution containing $200mg{\cdot}L^{-1}$ HQS disappeared at a holding solution containing $100mg{\cdot}L^{-1}$ HQS. The holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA extended the most effective treatments on vase life, fresh weight, water balance, and flowering of cut iris flowers. However, the holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI and 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI was not effective in solution uptake or transpiration, but did result in high water balance. Iris flowers treated with CHI at the half-open flower stage showed increases in ornamental value, such as full flower opening and extended vase life. To improve flower quality and prolonging vase life of cut iris flowers, a holding solution containing $50{\mu}M$ CHI can be used continuously from the half-open stage.

Japanese Companies Investment in China After Chinas Entry into WTO (중국(中國)의 WTO가맹(加盟)에 의한 일본기업(日本企業)의 대중투자(對中投資)에 관한 연구(硏究))

  • Wang, Guo An;Choi, Seok-Beom
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.23
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    • pp.341-372
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    • 2004
  • Since China was admitted to the World Trade Organization (WTO) on December 11, 2001, China has sped up its active participation in the competition of the world economy by lifting its trade and investment restrictions and limits. Its trading system is getting more and more free and its huge market with a population of 1.3 billion has been getting more and more open. With Chinas WTO accession, China has been honoring its commitments to its WTO members by complying with international business practices and WTO rules. For example, it will cut down its import tariffs for automobiles from the present 80% to 25% by 2006. Its financial sector and service industries are open to foreign investors now. As Chinas biggest business partner, Japan has benefited tremendously from Chinas open policies and deepening economic reform. In order to reduce the costs of production, a large number of enterprises from Japan have set up production bases in China since a Chinese workers wages accounts for only one tenth of those of a Japanese worker on the average. Japan has become one of Chinas biggest investors on a par with the United States of America (USA). How are the Japanese enterprises in Japan and in China adjusting themselves to the rapid changes of the Chinese market? What characteristics do the Japanese enterprises in China have? What effects has Chinas WTO accession had on those Japanese enterprises that have invested in China? This paper finds answers to the above questions. It at first reviews the Sino-Japanese economic and trade relations since 1980s, then analyses the situation and characteristics of Japanese-invested enterprises in China, and explores their problems in China. Finally it puts forward some suggestions for the Japanese enterprises in China.

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