• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.582-588
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• 1999

A Brevibacterium lactofermentum gene coding for a glucose-specific permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned, by complementing an Escherichia coli mutation affecting a ptsG gene with the B. lactofermentum genomic library, and completely sequenced. The gene was identified as a ptsG, which enables an E. coli transformant to transport non-metabolizable glucose analogue 2-deoxyglucose (2DG). The ptsG gene of B. lactofermentum consists of an open reading frame of 2,025 nucleotides encoding a polypeptide of 674 amino acid residues and a TAA stop codon. The 3' flanking region contains two stem-loop structures which may be involved in transcriptional termination. The deduced amino acid sequence of the B. lactofermentum enzyme $II^{GIe}$ specific to glucose ($EII^{GIe}$) has a high homology with the Corynebacterium glutamicum enzyme $II^{Man}$ specific to glucose and mannose ($EII^{Man}$), and the Brevibacterium ammoniagenes enzyme $II^{GIc}$ specific to glucose ($EII^{GIc}$). The 171-amino-acid C-terminal sequence of the $EII^{Glc}$ is also similar to the Escherichia coli enzyme $IIA^{GIc}$ specific to glucose ($IIA^{GIc}$). It is interesting that the arrangement of the structural domains, IIBCA, of the B. lactofermentum $EII^{GIc}$ protein is identical to that of EIIs specific to sucrose or $\beta$-glucoside. Several in vivo complementation studies indicated that the B. lactofermentum $EII^{Glc}$ protein could replace both $EII^{ Glc}$ and $EIIA^{Glc}$ in an E. coli ptsG mutant or crr mutant, respectively.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.34 no.2
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• pp.36-47
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• 2006

The process of dual spin turn maneuver is introduced for attitude acquisition or recovery from flat spin state of a satellite. The physical principle of momentum transfer during dual spin turn is explained clearly. The case studies of special dual spin turn, in addition to the conventional dual spin turn, that are known as an acceptable cases, are performed to investigate the principle of dual spin turn and to provide a physical insight as well as the solution of dual spin turn. This study is done based on case-study simulation, which includes two-state control scheme composed of open-loop maneuver and energy dissipation device. Furthermore, we investigate the stability for the verification of all control cases after implementing two-stage control. We also provide the simulation scenario of flat spin recovery using dual spin turn method as an example.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.8
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• pp.1776-1782
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• 2010

The interferometric fiber optic gyroscopes (FOGs) are well known as sensors of rotation, which are based on Sagnac effect, and have been under development for a number of years to meet a wide range of performance requirements. This paper describes the development of open-loop FOG and digital signal processing techniques implemented on FPGA. Our primary goal was to obtain intermediate accuracy (pointing grade) with a good bias stability (0.22deg) and scale factor stability, extremely low angle random walk (0.07deg) and significant cost savings by using a single mode fiber. A secondary goal is to design all digital FOG signal processing algorithms with which the SNR at the digital demodulator output is enhanced substantially due to processing gain. The Cascaded integrator bomb(CIC) type of decimation filter only requires adders and shift registers, low cost processors which has low computing power still can used in this all digital FOG processor.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.8
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• pp.1885-1894
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• 2014

There are various high-performance algorithms in the area of the existing VDSs (vehicle detection systems). However, they requires a large amount of computational time-complexity and their systems generally are very expensive and consumes high-power. This paper proposes real-time traffic information detection algorithm that can be applied to low-cost, low-power, and open development platform such as Android. This algorithm uses a vehicle's sub-shadow to set ROI(region of interest) and to count vehicles using a location of the sub-shadow and the vehicle. The proposed algorithm is able to count the vehicles per each roads and each directions separately. The experiment result show that the detection rate for going-up vehicles is 94.1% and that for going-down vehicles is 97.1%. These results are close to or surpasses 95%, the detection rate of commercial loop detectors.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.2
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• pp.187-191
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• 2004

Actin is a ubiquitous and highly conserved protein found in eukaryotic organisms. In this study, we describe the cDNA cloning and mRNA expression of an actin gene from the mulberry longicorn beetle, Apriona germari. The A. germari actin cDNA is 1524 bp containing a complete 1128 bp open reading frame that encodes a polypeptide of 376 amino acid residues with a predicted molecular weight of about 41.5 kDa. The deduced amino acid sequence of the A.germari actin cDNA showed 99% protein sequence identity to Homalodisca coagulata actin, differing at only two amino acid positions, and 92-98% protein sequence identity to known insect species actins. The predicted three-dimensional structure of A. germari actin revealed the four residue hydrophobic pulg loop characteristic of the actin family. Northern blot analysis showed that A. germari actin is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body of A. germari larva.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.3
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• pp.30-37
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• 2004

This thesis develops a fuzzy-PID control algorithm for control the filament winding tension. It is developed by applying classical PID control technique to a fuzzy logic controller. It is composed of a fuzzy-PI controller and a fuzzy-D controller. The fuzzy-PI controller uses error and integrated error as inputs, and the fuzzy-D controller uses derivative of error as input. The fuzzy-PI controller uses Takagi-Sugeno fuzzy inference system, and the fuzzy-D controller uses Mamdani fuzzy inference system. The fuzzy rule base for the fuzzy-PI controller is designed using 19 rules, and the fuzzy rule base for the fuzzy-D controller is designed using 5 rules. A test-bed is set-up for verifying the effectiveness of the developing control algorithm in control the filament winding tension. It is composed of a mandrel, a carriage, a force sensor, a driving roller, nip rollers, a creel, and a real-time control system. Nip rollers apply a vertical force to a filament, and the driving roller drives it. The real-time control system is developed by using MATLAB/xPC Target. First, experiments for showing the inherent problems of an open-loop control scheme in a filament winding are performed. Then, experiments for showing the robustness of the developing fuzzy-PID control algorithm are performed under various working conditions occurring in a filament winding such as mandrel rotating speed change, carriage traversing, spool radius change, and reference input change.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.12
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• pp.1529-1534
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• 2013

A voice coil actuator with a rotating moving magnet has been developed for a miniaturized mobile robot. The actuator has simple structure comprising a magnet, a coil, and a yoke. Actuator performance is predicted using a linearized theoretical model, and dynamic performance based on the air-gap between the magnet and the coil is predicted using motor constant and restoring constant obtained through finite element simulations. The theoretical model was verified using a prototype with 60 Hz resonance and 80 Hz bandwidth. We found that an input of 1.5 V can make the actuator rotate by $20^{\circ}$ statically. The driving configuration of the proposed actuator can be simplified because of its implementation of open-loop control.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.153-156
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• 2009

The interferometric fiber optic gyroscopes (FOGs) are well known as sensors of rotation, which are based on Sagnac effect, and have been under development for a number of years to meet a wide range of performance requirements. This paper describes the development of open-loop FOG and digital signal processing techniques implemented on FPGA. Our primary goal was to obtain intermediate accuracy (pointing grade) with a good bias stability ($0.22^{\circ}/hr$) and scale factor stability, extremely low angle random walk ($0.07^{\circ}/\sqrt{hr}$) and significant cost savings by using a single mode fiber. A secondary goal is to design all digital FOG signal processing algorithms with which the SNR at the digital demodulator output is enhanced substantially due to processing gain. The CIC type of decimation block only requires adders and shift registers, low cost processors which has low computing power still can used in this all digital FOG processor.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.10a
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• pp.267-273
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• 2004

This paper targets to define controlling factors of pressure-coupled combustion response and estimate their effects on droplet evaporation process. Dynamic characteristics of hydrocarbon propellant vaporization perturbed by acoustic pressure are numerically simulated and analyzed. 1-D droplet model including phase equilibrium between two phases is applied and acoustic wave is expressed by harmonic function. Effects of various design factors and acoustic pressure on combustion response are investigated with parametric studies. Results show that driving frequency of acoustic perturbation and ambient pressure have important roles in determining magnitude and phase of combustion response. On the other hand, other parameters such as gas temperature, initial droplet size and temperature, and amplitude of acoustic wave cause only minor changes to magnitude of combustion response. Resultant changes in phase of heat of vaporization and thermal wave in droplet highly influence magnitude and phase of combustion response.

• Journal of the Korean Society of Propulsion Engineers
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• v.19 no.3
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• pp.20-28
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• 2015

Performance parameters of solid rocket motor with multi axis pintle nozzles were analyzed theoretically and modeled. For figuring out the governed variable of dynamic characteristics of system, dynamic analysis was done by using established model. To present characteristics of this system, the model should include not only internal ballistics of propulsion unit but also actuating system to move pintle. For solid rocket motor with multi axis pintle nozzles, not only performance of steady state but also dynamic characteristic of transient state is important design parameter to precise thrust control. Therefore, response time of open-loop system was analyzed by using established model and requirement about response time was satisfied by controlling pressure.