• Korean Journal of Environmental Agriculture
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• v.7 no.2
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• pp.111-116
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• 1988

The aquatic plants were cultivated in pots containing pig waste water, adjusted to three levels of $NH_4-N$ concentration 50, 100 and 200ppm. The aquatic plants were Eichhornia crassipes solms-laub, Monochoria korsakowii Regel et maack, Zizania caduciflora, Typha orientalis, Acorus asiaticus, Cyperus exaltaus, Colocasia antiquorum var. Yield, content and amount of nitrogen and phosphorus absorped by plants, and growth status were investigated. The results obtained are as follows. 1. The content and removal amount of nitrogen and phosphrus by plants were the highest in Eichhornia crassipes solms-laub. 2. Yield of dry matter in plants in 100ppm $NH_4-N$ was in the order of Eichhornia crassipes solms-laub>Zizania caduciflora>Typha orientalis>Monochoria korsakowii Regel et maack>Acorus asiaticus. 3. The removal amount of nitrogen by plants in the 100ppm $NH_4-N$ was in the order of Eichhornia crassipes solms-laub>Zizania caduciflora>Monochoria korsakowii Regel et maack>Typha orientalis>Acorus asiaticus>Colocasia antiquorum var>Cyperus exaltatus. Removal amount of phosphorus was in the order of Eichhornia cras sipes solms-laub>Monochoria korsakowii Regel et maack>Zizania caducilora>Typha orientalis>Acorus asiaticus> Cyperus exaltatus>Colocasia antiquorum var. 4. Concentration causing growth damage was 200ppm of $NH_4-N$ in Eichhornia crassipes solms-laub, Zizania caduciflora and Typha orientalis and 100ppm in Monochoria korsakowii Regel et maack and Acorus asiaticus. 5. Nitrogen content was the highest in leaf and phosphorus content was the highest in float of the water hyacinth. 6. The number of panicles of the water hyacinth increased by 752 pieces and dry matters were about 5,000kg/l0a during one year.

• Research in Plant Disease
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• v.18 no.2
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• pp.109-116
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• 2012

A bacterial strain antagonistic to some fungal phytopathogens was isolated from the stem of a Persimmon tree in Yeongam, Korea. This bacterium was identified as Bacillus subtilis by 16S rRNA gene sequencing and designated as B. subtilis GDYA-1. In in vivo experiment, the fermentation broth exhibited antifungal activities against Magnaporthe oryzae on rice plants, Phytophthora infestans on tomato plants, and Puccinia recondita on wheat plants. We isolated one antifungal compound and its chemical structure was determined by mass and $^1H$-NMR spectral data. The antifungal substance was identified as benzoic acid. It inhibited mycelial growth of M. oryzae, Rhizoctonia solani, Sclerotinia sclerotiorum, and P. capsici with minimum inhibition concentration (MIC) values, ranging from 62.5 to 125 ${\mu}g/ml$. Moreover, the substance effectively suppressed Phytophthora blight of red pepper caused by P. capsici in a pot experiment. To the author's knowledge, this is the first report on the antifungal activity of benzoic acid against phytopathogenic fungi. Benzoic acid and B. subtilis GDYA-1 may contribute to environmental-friendly protect crops from phytopathogenic fungi.

• Research in Plant Disease
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• v.18 no.2
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• pp.86-92
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• 2012

Clubroot caused by Plasmodiophora brassicae Woron. is one of the most important diseases in Brassica crops worldwide. To establish more simple and reliable screening method for resistant cabbage and broccoli to P. brassicae, the development of clubroot on the plants according to inoculum concentration and incubation period after inoculating with the pathogen was investigated using P. brassicae GN1 isolate (race 9). To facilitate and acquire precise result of resistance screening of cabbage and broccoli to clubroot, 14-day-old seedlings were inoculated by drenching roots with the spore suspension of P. brassicae to give inoculum density of $2.5{\times}10^9$ spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days, and then cultivated in a greenhouse ($20{\pm}5^{\circ}C$) for five weeks. Under the optimum conditions, 16 cabbage and 17 broccoli cultivars were tested for resistance to four field isolates (GN1, GN2, GS and YC) of P. brassicae collected from four regions in Korea. Among them, some cabbage and broccoli cultivars showed different resistance response to three isolates (GN1, GN2 and GS) determined as race 9 by using the differential varieties of Williams. On the other hand, all the tested cultivars were highly susceptible to YC isolate (race 2). The results suggest that this method is efficient screening method of cabbage and broccoli for resistance to P. brassicae.

• The Plant Pathology Journal
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• v.26 no.4
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• pp.313-320
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• 2010

Genetic instability of the rice blast fungus Magnaporthe oryzae has been suggested as a major factor underlying the rapid breakdown of host resistance in the field. However, little information is available on the mechanism of genetic instability. In this study, we assessed the stability of repetitive DNA elements and several key phenotypic traits important for pathogenesis after serially transferring two isolates though rice plants and an artificial medium. Using isolate 70-15, we obtained a total of 176 single-spore isolates from 10 successive rounds of culturing on artificial medium. Another 20 isolates were obtained from germ tubes formed at the basal and apical cells of 10 three-celled conidia. Additionally, 60 isolates were obtained from isolate KJ201 after serial transfers through rice plants and an artificial medium. No apparent differences in phenotypes, including mycelial growth, conidial morphologies, conidiation, conidial germination, appressorium formation, and virulence, or in DNA fingerprints using MGR586, MAGGY, Pot2, LINE, MG-SINE and PWL2 as probes were observed among isolates from the same parent isolate. Southern hybridization and sequence analysis of two avirulence genes, AVR-Pita1 and AVR-Pikm, showed that both genes were also maintained stably during 10 successive generations on medium and plants. However, one reversible loss of restriction fragments was found in the telomere-linked helicase gene (TLH1) family, suggesting some telomere regions may be more unstable than the rest of the genome. Taken together, our results suggest that phenotype and genotype of M. oryzae isolates do not noticeably change, at least up to 10 successive generations on a cultural medium and in host plants.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.2
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• pp.105-111
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• 2013

The experiments were carried out to investigate the biocontrol potential of Lysobacter capsici YS1215 on root-knot nematode and to characterize its lytic enzyme activities. L. capsici YS1215 showed chitinase and gelatinase activities on the medium containing 0.5% chitin or 0.5% gelatin as substrates. Cell growth of L. capsici YS1215 was highest at 6 days, and the highest activities of chitinase (4.0 unit $ml^{-1}$) and gelatinase (7.43 unit $ml^{-1}$) were observed on 3 and 5 days after incubation, respectively. To investigate the effect of L. capsici YS1215 on tomato growth and nematode infection, the plants in pot trial were treated with bacterial culture (BC), half of bacterial culture (HBC), only bacterial medium (BM), tap water (TW) and commercial nematicide (CN). HBC treatd plants showed the higher shoot fresh weight and dry weight on $5^{th}$week after incubation while BM, HBC and BC had consistently higher values than TW at $9^{th}$ week. HBC appeared to be the highest shoot fresh length at $9^{th}$ week. Both CN and BC showed lower number of egg mass, root gall, and population of juveniles in soil compared to BC, HBC, BM and TW. These results suggest that L. capsici YS1215 with its strong ability of lytic enzyme production can be one of the most significant candidates for biocontrol agents against root-knot nematodes.

• Journal of Wetlands Research
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• v.17 no.2
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• pp.203-208
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• 2015

To identify the effect of fallen cherry blossom on the artificial pond ecosystem, microcosm experiment was conducted into the aquatic decomposition of Prunus species petals. Petals were put in $1mm^2$ mesh nylon litter bags. For treatment group, one flower litter bag was placed into each pot microcosm ($27{\times}20{\times}8cm^3$) filled with influent water from the artificial pond, whereas control group microcosm contained pond water only. Decomposition time were set differently (4, 8, 12, 16 days) among treatment groups. At the end of experiment, most petals were decomposed and only 32.3% of initial dry weight remained with the decay rate (k) of $7.06{\times}10^{-2}day^{-1}$. $NO_3-N$ concentration of microcosm water decreased sharply from 1.90 mg/L at first to 0.02 mg/L, whereas $NH_4-N$ concentration increased from 0.03 mg/L to 2.85 mg/L continually. $PO_4-P$ concentration was 0.03 mg/L at first and increased to 2.39 mg/L by decomposition. Therefore, available phosphorus seems to have leached with higher rate than nitrogen from the petals litter. Increase about 0.02 mg/L in $PO_4-P$ concentration could be estimated in artificial pond from the calculation on the total quantity of fallen blossoms. This result suggests that available phosphorus from the decomposed Prunus petals could cause eutrophication in the artificial pond.

• Korean Journal of Weed Science
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• v.18 no.2
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• pp.106-115
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• 1998

This study was conducted to investigate the effect of oxadiazon, molinate, thiobencarb before seeding on control of red rice and barnyardgrass in water-seeded rice. High application rate plot among oxadiazon treatment plots could observe phytotoxicity symptoms depending on field conditions, but these injury recovered gradually with time. Molinate and thiobencarb application plots at the concentration of 225~400, 210~420g ai/l0a respectively were not observed phytotoxicity. Control of red rice was different according to kinds of herbicides and application rates. Oxadiazon showed higher control performance at the concentration of more than 60g ai/10a. Control effect of molinate and thiobencarb against red rice was enhanced with the increase of application rate, and both herbicides showed satisfactory effect at more than 300g ai/10a. Control of barnyardgrass showed up to 90~100% in all tested herbicides. There was no significant yield reduction by oxadiazon, molinate, and thiobencarb application before seeding in all tested field. In the pot experiment, crop injury, seedling stand, and early growth were more advantageous at time of drainge after one day after seeding than flooding until rooting.

• FLOWER RESEARCH JOURNAL
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• v.17 no.2
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• pp.81-87
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• 2009

This study was conducted to examine the characteristics of flowering and fruiting in Nelumbo pentapetala 'Yellow Bird'. Lotus rhizomes were planted in pot (70 cm in length and 71 cm in width) on April 18, 2008, and grown under rain shelter condition at a private farm in Muan-gun, Jeonnam. Ninety percent of the lotus plants were bloomed as two flowers per a plant, and the others were one flower. The first flowers were bloomed in the middle or late July, and the second flowers in the early August. Flower stalks were formed between eighth and tenth internodes in the main rhizome. Nelumbo pentapetala 'Yellow Bird' had light yellow flowers, 12 cm in length and 13 cm in width. Lotus plants of 21.4% were not bloomed, resulting from the insufficient securing of internodes in the main rhizome and the blind. We, therefore, concluded that Nelumbo pentapetala 'Yellow Bird' as a ornamental plant should be grown up to 8 in the node number of main rhizome until mid-July.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.238-238
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• 2015

There are demands for water environmental analysis of discharge processes in paddy fields, however, it is not fully understood in nutrients discharge process for watershed modeling. As hydrological processes both surface and ground water and agricultural water managements are so complex in paddy fields, the development of lowland paddy fields watershed model is more difficult than upland watershed model. In this research, the improvement of SWAT (Soil and Water Assessment Tool) model for a paddy watershed was conducted. First, modification of surface inundated process was developed in improved pot hole option. Those modification was evaluated by monitoring data. Second, the monitoring data in river and drainage channel in lowland paddy fields from 2012 to 2014 were analyzed to understand discharge characteristics. As a case study, Imbanuma basin, Japan, was chosen as typical land and water use in Asian countries. In this basin, lowland paddy fields are irrigated from river water using small pumps that were located in distribution within the watershed. Daily hydrological fluctuation was too complex to estimate. Then, to understand surface and ground water discharge characteristics in irrigation (Apr-Aug) and non-irrigation (Sep-Mar) period, the water and material balance analysis was conducted. The analysis was composed two parts, watershed and river channel blocks. As results of model simulation, output was satisfactory in NSE, but uncertainty was large. It would be coming from discharge process in return water. The river water and ground water in paddy fields were exchanged each other in 5.7% and 10.8% to river discharge in irrigation and non-irrigation periods, respectively. Through this exchange, nutrient loads were exchanged between river and paddy fields components. It suggested that discharge from paddy fields was not only responded to rainfall but dynamically related with river water table. In general, hydrological models is assumed that a discharge process is one way from watershed to river. However, in lowland paddy fields, discharge process is dynamically changed. This function of paddy fields showed that flood was mitigated and temporally held as storage in ground water. Then, it showed that water quality was changed in mitigated function in the water exchange process in lowland paddy fields. In future, it was expected that hydrological models for lowland paddy fields would be developed with this mitigation function.

• Korean Journal of Soil Science and Fertilizer
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• v.8 no.4
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• pp.177-181
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• 1975

The pot experiment was conducted primarily to clarify the relationship between lime application and they amount of potassium for the basal and top dressings on paddy rice. (Orizae sativa L. cultivar; variety Jinheung) The soils mixed with lime and without lime were separately filled in to 0.2827 a. open-bottom concretepots. The soil with lime was treated with slaked lime, at two weeks before transplanting to correct soil pH. around 7.0. The total amount of potassium 12 kilograms per 10a was applied by two or three split doses during transplanting, 19 days after transplanting and primodial stages. The results of experiment obtained are summarized as follows: 1. The application of potash in the rate of 4kg per 10a or less for basal dressing and the remaining amount of potash for top dressing at 19 days after transplanting and primodial stage or one top dressing, at primodial stage have increased the yield of paddy with increased number of panicles per hill, milliequivalent ratio of $K^-/Ca^{++}+Mg^{++}$ in leaves in the later stage of growth, number of ripened grains per panicle, and rate of grain maturity. 2. On the other hand, the greater the amount of potassium given for the basal dressing (over 4kilograms per 10a), the smaller number of tillers and panicles were resulted. And the tendency was considered chiefly due to high salt concentration at the early stage of rice growth.