Browse > Article
http://dx.doi.org/10.7745/KJSSF.2013.46.2.105

Biological Control of Root-knot Nematode by Lysobacter capsici YS1215  

Lee, Yong-Sung (Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (202), Chonnam National University)
Park, Yun-Suk (Purne Co., Ltd., Institute of Environmentally-Friendly Agriculture, Chonnam National University)
Kim, Sun-Bae (Damyang Agriculture Technical Center)
Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture (202), Chonnam National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.46, no.2, 2013 , pp. 105-111 More about this Journal
Abstract
The experiments were carried out to investigate the biocontrol potential of Lysobacter capsici YS1215 on root-knot nematode and to characterize its lytic enzyme activities. L. capsici YS1215 showed chitinase and gelatinase activities on the medium containing 0.5% chitin or 0.5% gelatin as substrates. Cell growth of L. capsici YS1215 was highest at 6 days, and the highest activities of chitinase (4.0 unit $ml^{-1}$) and gelatinase (7.43 unit $ml^{-1}$) were observed on 3 and 5 days after incubation, respectively. To investigate the effect of L. capsici YS1215 on tomato growth and nematode infection, the plants in pot trial were treated with bacterial culture (BC), half of bacterial culture (HBC), only bacterial medium (BM), tap water (TW) and commercial nematicide (CN). HBC treatd plants showed the higher shoot fresh weight and dry weight on $5^{th}$week after incubation while BM, HBC and BC had consistently higher values than TW at $9^{th}$ week. HBC appeared to be the highest shoot fresh length at $9^{th}$ week. Both CN and BC showed lower number of egg mass, root gall, and population of juveniles in soil compared to BC, HBC, BM and TW. These results suggest that L. capsici YS1215 with its strong ability of lytic enzyme production can be one of the most significant candidates for biocontrol agents against root-knot nematodes.
Keywords
Biological control; Lysobacter capsici YS1215; Root-knot nematodes; Chitinase; Gelatinase;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Mandl, I., J.D. MacLennan, E.L. Howes, R.H. DeBellis, and A. Sohler. 1953. Isolation and characterization of proteinase and collagenase from Cl. histolyticum. J Clin Invest 32:1323-1329.   DOI   ScienceOn
2 Moore, S. and W.H. Stein. 1948. Photometric ninhydrin method for use in the chromatography of amino acids. J. Biol. Chem. 176:367-388
3 Oka, Y., H. Koltai, M. Bar-Eyal, M. Mor, E. Sharon, I. Chet, and Y. Spiegel. 2000. New strategies for the control of plant-parasitic nemaotdes. Pest Manag Sci 56:983-988.   DOI   ScienceOn
4 Park, M.H., J.K. Kim, W.H. Choi, and M.H. Yoon. 2001. Nematicidal effect of root-knot nematode (Meloidogyne incognita) by biological nematicide. Korean J. Soil Sci. Fert. 44(2):228-235.
5 Park, J.H., R. Kim, Z. Aslam, C.O. Jeon, and Y.R. Chung. 2008. Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter. Int J Syst Evol Microbiol 58:387-392.   DOI   ScienceOn
6 Sasser, J.N. 1980. Root-knot nematode: a global menace to crop production. Plant Dis. 64:36-41.   DOI
7 Sela, S., H. Schickler, I. Chet, and Y. Spigel. 1998. Purification and characterization of Bacillus cereus collagenolytic/proteolytic enzyme and its effect on Meloidogyne javanica cuticular proteins. Eur. J. Plant. Pathol. 104:59-67.   DOI   ScienceOn
8 Siddiqui, Z.A. and I. Mahmood. 1999. Role of bacteria in the management of plant parasitic nematodes: a review. Bioresource Technol. 69:167-179.   DOI   ScienceOn
9 Tunlid, A. and S. Janson. 1991. Proteases and their involvement in the infection and immobilization of nematode by the nematophagous fungus Arthrobotrys oligospora. App. Environ. Microbiol. 57:2868-2872.
10 Yoon, G.Y., Y.S. Lee, S.Y. Lee, R.D. Park, H.N. Hyun, Y. Nam, and K.Y. Kim. 2012. Effects on Meloidogyne incognita of chitinase, glucanase and a secondary metabolite from Streptomyces cacaoi GY525. Nematology 14(2): 175-184.   DOI   ScienceOn
11 Zhu, Y.Z., D.S. Park, M.R. Cho, J.H. Hur, and C.K. Lim. 2005. Suppression of Meloidogyne arenaria by different treatments of Pasteuria penetrans. J. Pestic. Sci. 9(4):437-441.
12 Dicklow, M.B., N. Acosta, and B.M. Zuchkerman. 1993. A novel Streptomyces species for controlling plant-parasitic nematodes. J. Chem. Ecol. 19:159-173.   DOI   ScienceOn
13 Christensen, P. and D. Cook. 1978. Lysobacter, a new genus of non-fruiting, gliding bacteria with a high base ratio (soil and water organisms). Int. J. Syst. Bacteriol. 28:367-393.   DOI
14 Choi, Y.E. and Y.J. La. 1982. Plant nematology. pp.58-68.
15 Devidas, P. and L.A. Rehberger. 1992. The effects of exotoxin (thuringiensin) from Bacillus thuringiensis on Meloidogyne incognita and Caenorhabditis elegans. Plant Soil 145: 115-120.   DOI
16 Gautam, A., Z.A. Siddiqui, and I. Mahmood. 1995. Intergated management of Meloidogyne incognita on tomato. Nematologia Mediterranea 23:245-247.
17 Kang, S.J., Y.S. Lee, S.Y. Lee, G.Y. Yun, S.H. Hong, Y.S. Park, I.S. Kim, R.D. Park, and K.Y. Kim. 2010. Biological Control of Diamondback Moth (Plutella xylostella L.) by Lysobacter antibioticus HS124. Korean J. Soil Sci. Fert. 43(5):537-544 (2010)
18 Kim, D.J. and K.J. Choi. 2001. Effects of incorporation method of nematicides on reproduction of Meloidogyne arenaria. Kor. J. Appl. Entomol. 40:89-95.
19 Kim, J.I. and S.C. Han. 1988. Effect of solarization for control of root-knot nematode (Meloidogyne spp.) in vinyl house. Kor. J. Appl. Entomol. 27: 1-5.
20 Kim, S.S., S.I. Kang, J.S. Kim, Y.S. Lee, S.H. Hong, W.N. Kyaw, and K.Y. Kim. 2011. Biological control of root-knot nematode by Streptomyces sampsonii KK1024. Korean J. Soil Sci. Fert. 44(6):1150-1157.   DOI   ScienceOn
21 Ko, H.S., R.D. Jin, B.H. Krishnan, S.B. Lee, and K.Y. Kim. 2009. Biocontrol Ability of Lysobacter antibioticus HS124 Against Phytophthora Blight Is Mediated by the Production of 4-Hydroxyphenylacetic Acid and Several Lytic Enzymes. CurrMicrobiol 59:608-615.
22 Lingapa, Y. and J.L. Lockwood. 1962. Chitin media for selected isolation and culture of Actinomycetes. Phytopathology 52:317-323.
23 Kwon, T.Y., K.C. Jung, S.D. Park, Y.G. Sim, and B.S. Choi. 1998. Cultural and chemical control of root-knot nematodes, Meloidogyne sp. on oriental melon in plastic film house. RDA J. Crop Prot. 40:96-101.
24 Lee, J.G. 2003. Occurrence, ecology and control of root-knot nematodes under greenhouse cultivation system. Ph.D. Thesis, Chungnam National University, Daejeon, Korea.
25 Lee, J.S., H.Y, Choo, and D.W. Lee. 2011. Nematicidal Efficacy of Herbal Extracts against Meloidogyne Hpala. Kor. J. Appl. Entomol. 50(4): 315-324.   DOI   ScienceOn
26 Li, W., D.P. Roberts, P.D. Derby, S.L.F. Meyer, S. Lohrke, R.D. Lumsden, and K.P. Hebbar. 2002. Broad spectrum anti-biotic activity and disease suppression by the potential biocontrol agent Burkholderia ambifaria BC-F. Crop Prot. 21:129-135.   DOI   ScienceOn
27 Lim, S.H., Y.Z. Zhu, M.S. Kim, Y.S. Lee, J.S. Son, D.S. Park, J.H. Hur, H.Y. Kim, H.J. Choi, K.H. Kim, and S.M. Kim. 2004. Nematicidal activity of Korean native plants against root-knot nematode, Meloidogyne incognita. Kor. J. Pestic. Sci. 8:353-357.
28 Abo-Elyousr, K.A., Z. Khan, M.E. Award, and M.F. Abedel-Moneim. 2010. Evaluation of plant extracts and Pseudomonas spp. for control of root-knot nematode, Meloidogyne incognita on tomato. Nematropica 40(2):289-299.
29 Atkins, S.D., L. Hidalgo-Diaz, H. Kalisz, T.H. Mauchline, P.R. Hirsch, and B.R. Kerry. 2003. Development of a new management strategy for the control of root-knot nematodes (Meloidogyne spp.) in organic vegetable production. Pest Manag Sci. 59:183-189.   DOI   ScienceOn
30 Birch, A.N.E., W.M. Robertson, and L.E. Fellows. 1993. Plant products to control plant parasitic nematodes. Pestic. Sci. 39: 141-145.   DOI   ScienceOn
31 Chen, J., W.H. Moore, G.Y., Yuen, D. Kobayashi, and E.P. Caswell-Chen. 2006. Influence of Lysobacter enzymogenes Strain C3 on Nematodes. J Nematol 38(2):233-239.
32 Broadbent, P., K.F Baker, N. Franks, and J. Holland. 1977. Effect of Bacillus spp. on increased growth of seedling in steamed and in non treated soil. Phytopathology 67:10271034.