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http://dx.doi.org/10.5423/RPD.2012.18.2.109

Antifungal Activity of Benzoic Acid from Bacillus subtilis GDYA-1 against Fungal Phytopathogens  

Yoon, Mi-Young (Ecofriendly New Materials Research Group, Korea Research Institute of Chemical Technology)
Seo, Kook-Hwa (Green Dynamics Corp.)
Lee, Sang-Heon (Green Dynamics Corp.)
Choi, Gyung-Ja (Ecofriendly New Materials Research Group, Korea Research Institute of Chemical Technology)
Jang, Kyoung-Soo (Ecofriendly New Materials Research Group, Korea Research Institute of Chemical Technology)
Choi, Yong-Ho (Ecofriendly New Materials Research Group, Korea Research Institute of Chemical Technology)
Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
Kim, Jin-Cheol (Ecofriendly New Materials Research Group, Korea Research Institute of Chemical Technology)
Publication Information
Research in Plant Disease / v.18, no.2, 2012 , pp. 109-116 More about this Journal
Abstract
A bacterial strain antagonistic to some fungal phytopathogens was isolated from the stem of a Persimmon tree in Yeongam, Korea. This bacterium was identified as Bacillus subtilis by 16S rRNA gene sequencing and designated as B. subtilis GDYA-1. In in vivo experiment, the fermentation broth exhibited antifungal activities against Magnaporthe oryzae on rice plants, Phytophthora infestans on tomato plants, and Puccinia recondita on wheat plants. We isolated one antifungal compound and its chemical structure was determined by mass and $^1H$-NMR spectral data. The antifungal substance was identified as benzoic acid. It inhibited mycelial growth of M. oryzae, Rhizoctonia solani, Sclerotinia sclerotiorum, and P. capsici with minimum inhibition concentration (MIC) values, ranging from 62.5 to 125 ${\mu}g/ml$. Moreover, the substance effectively suppressed Phytophthora blight of red pepper caused by P. capsici in a pot experiment. To the author's knowledge, this is the first report on the antifungal activity of benzoic acid against phytopathogenic fungi. Benzoic acid and B. subtilis GDYA-1 may contribute to environmental-friendly protect crops from phytopathogenic fungi.
Keywords
Biological control; Biopesticides; Plant diseases;
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