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Commercialization of Genetically Modified Ornamental Plants

  • Chandler, Stephen F.
    • Proceedings of the Korean Society of Plant Biotechnology Conference
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    • 2003.04a
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    • pp.39-48
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    • 2003
  • The ornamental industry encompasses cut flower, pot plant, turfgrass and nursery stock production and is an important part of the agricultural sector. As internationally traded commodities, cut flowers and plants are an integral part of the economy of a number of developing countries in South America, the Caribbean and Africa. Genetic modification (GM) is a tool with great potential to the ornamental horticulture industry. The rapid progress in our knowledge of plant molecular biology can accelerate the breeding ornamental plants using recombinant DNA technology techniques. Not only is there the possibility of creating new, novel products the driver of the industry but also the potential to develop varieties requiring less chemical and energy inputs. As an important non-food agricultural sector the use of genetically modified (GM) ornamental crops may also be ideal for the intensive farming necessary to generate pharmaceuticals and other useful products in GM plants. To date, there are only a few ornamental GM products in development and only one, a carnation genetically modified for flower colour, in the marketplace. International Flower Developments, a joint venture between Florigene Ltd. in Australia and Suntory Ltd.of Japan, developed the GM carnations. These flowers are currently on sale in USA, Japan and Australia. The research, development and commercialisation of these products are summarised. The long term prospects for ornamental GM products, like food crops, will be determined by the regulatory environment, and the acceptance of GM products in the marketplace. These critical factors will be analysed in the context of the current legislative environment, and likely public and industry opinion towards ornamental genetically modified organisms (GMO's).

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Uptake and Accumulation of Arsenate on Lettuce (Lactuca sativa L.) Grown in Soils Mixed with Various Rates of Arsenopyrite Gravel (유비철석 입자 혼합 토양내 상추(Lactuca sativa L.)의 비소이온 흡수와 축적)

  • Shim, Ho-Young;Lee, Kyo-Suk;Lee, Dong-Sung;Jeon, Dae-Sung;Shin, Ji-Su;Kim, Soo-Bin;Cho, Jin-Woong;Chung, Doug-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.532-538
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    • 2014
  • Arsenic (As) is nonessential element toxic to plants. In Korea little is not only known about the extent of actual anthropogenic sources and inputs of arsenic to the agricultural land which plays a active role as a sink, but also systematic research on arsenic as an toxic element entering the food chain via the soil-plant pathway has not been investigated in the fields and greenhouses besides in few places of abandoned mining sites. Therefore, it is important to focus on the effect of As-contaminated soils on As uptake and biomass production of lettuce plants. In this study, As concentrations in the soil and accumulation of As in lettuce transferred by As uptake from soils were investigated. To do this, soil which was mixed with various rates of arsenopyrite gravels containing arsenic from 0 to 100% was packed into a round plastic pot. Then, 10 days old vegetable crops of chinese cabbage and lettuce after germination were transplanted into a pot. Growth of lettuce was observed for four weeks with one week interval. All experiments were done by triplicate. The results showed that the growth rates for number of leaves, width and length of the crop plants were retarded with increasing amount of gravel mixed due to increasing bioavailable amount of arsenate with increasing rate of gravel in soils. With these results, we conclude that the bioavailable amount of arsenate can influence the growth of lettuce.

Changes in the Concentrations of the Tap Water Chlorination By-Products by Heating during Cooking, and Human Ingestion Exposure (조리시 가열에 따른 수돗물 중 염소소독부산물의 농도 변화와 인체 섭취 노출)

  • 김희갑;이수형
    • Environmental Analysis Health and Toxicology
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    • v.14 no.1_2
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    • pp.35-43
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    • 1999
  • A number of disinfection by-products (DBPs) are formed as a result of the addition of chlorine into the public water supply and some of them have been suggested to cause adverse health effects on humans. However, the estimation of human ingestion exposure to each DBP has been performed simply by multiplying the concentration of a chemical in the cold tap water by the volume of water consumed during a given period of time. However, a questionnaire concerning water consumptions administered to sixty people residing in Chunchon showed that the volume of tap water consumed accounted for approximately 70% of the total volume of water consumed and that of heated water represented approximately 94% of tap water ingested. Heating durations for water-containing foods (e. g., soups and pot stews) and heated beverages (e. g., barley tea) were grouped into 10, 20, 30, and 35 minutes. Based on these time frames, an aluminum pot containing one liter of tap water was heated for the above respective time periods using a gas range to determine the variations of the concentrations of individual DBPs by heating. The pH and total residual chlorine were measured before and after heating. Collected water samples were carried to the laboratory and analyzed for eight DBPs and total organic carbon. Chloroform, bromodichloromethane, chloral hydrate, 1, 2-dichloro-2-propanone, 1, 1, 1-trichloropropanone, and dichloroacetonitrile were not detected following heating for 10 minutes and longer. The concentration of dichloroacetic acid (DCAA) was elevated with heating duration, resulting in the averages of 2.0, 3.1, 4.7, and 12 times the initial concentration, respectively, for 10, 20, 30, and 35 minute heating periods. On the other hand, the concentration of trichloroacetic acid (TCAA) decreased with heating duration, with 0.65, 0.40, 0.34, and 0.19 times lower than the initial concentration. Therefore, it is suggested that ingestion exposure to DCAA increases with heating duration but that ingestion exposure to TCAA decreases. In addition, while the amount of DCAA was elevated at the initial time periods (10 or 20 minutes) and then slowly decreased, that of TCAA was rapidly decreased. In conclusion, water-heating processes during cooking influence the concentrations of individual DBPs in the tap water, with lower levels for volatile DBPs and TCAA, and higher levels for DCAA. Therefore, concentration change needs to be taken into consideration in the estimation of human ingestion exposure to DBPs.

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Evaluation of Sanitation Management Practices and Microbiological Quality of Foods in Kindergarten Foodservice Settings (유치원 급식의 위생관리 실태조사 및 미생물적 품질평가)

  • Lee, Joo-Eun;Choi, Kyung-Sook;Kang, Young-Jae;Kwak, Tong-Kung
    • Korean journal of food and cookery science
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    • v.28 no.5
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    • pp.515-530
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    • 2012
  • This research aims to audit foodservice sanitation management practices and to assess microbiological quality of foods and their food contact environments in kindergartens. Sanitation auditing was conducted in 10 kindergartens in Seoul, Gyeonggi, and Incheon areas to assess the levels of safety practices. Results revealed that the surveyed kindergartens scored 41.4 out of 100 points, on average. The average scores of each category were 6.4/11 (58.1%) for facilities sanitation, 4.2/12 (35.0%) for equipment sanitation, 2.4/10 (24.0%) for personal hygiene, 5.1/10 (51.0%) for food ingredients management, 6.0/17 (35.3%) for production process, 5.4/10 (54.0%) for environmental sanitation, 2.0/6 (33.3%) for kitchen utensils sanitation, and 2.2/6 (96.7%) for safety management. Microbiological quality of raw, prepared foods, personal sanitation (hands), environmental sanitation, and drinking water were assessed. Total plate counts (TPC) of the following menus exceeded the critical limit: seasoned leek (5 log CFU/g), cucumber (5.0 log CFU/g), panbroiled fish paste (TNTC at $10^4$), tangpyeongchae (5.3 log CFU/g), egg rolls (6.1 log CFU/g), panbroiled sausage (TNTC at $10^4$), and soft tofu pot stew (TNTC at $10^4$). Coliform which exceeded the standard limit were detected from seasoned leek (2 log CFU/g), cucumber (2.5 log CFU/g), panbroiled fish paste (2.0 log CFU/g), egg roll (3.8 log CFU/g), tangpyeongchae (4.0 log CFU/g), panbroiled sausage (2.3 log CFU/g), and soft tofu pot stew (3.7 log CFU/g). For seasoned foods (muchim), S. aureus ranged 2.2~2.9 log CFU/g. In food workers' hands, microbial profiles ranged 3.8~7.9 log CFU/hand for TPC, ND~4.5 log CFU/hand for coliforms, ND~4.7 log CFU/hand for S. aureus, and ND~5.3 log CFU/hand for Enterobacteriaceae. Microbiological profiles of food contact surface of knives, cutting boards, dish-clothes, and trays showed possibilities of cross-contamination. General bacteria were 2.1~4.5 logCFU/ml in 4 purified water samples and E. coli were found in the kitchen of one kindergarten. These results suggested that environmental sanitation management practices need more strict improvement: effective sanitation education methods and practices were strongly required, and more strict sanitation management for cooking utensils and equipment were required.

Effects of Irrigation and Ginseng Root Residue on Root Rot Disease of 2-Years-Old Ginseng and Soil Microbial Community in the Continuous Cropping Soil of Ginseng (인삼 연작토양에서 관수 및 인삼뿌리 잔사물이 토양 미생물상 및 뿌리썩음병 발생에 미치는 영향)

  • Lee, Sung Woo;Lee, Seung Ho;Seo, Moon Won;Park, Kyung Hoon;Jang, In Bok
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.5
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    • pp.345-353
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    • 2018
  • Background: Some phenolics detected in the soil may inhibit the seed germination and seedling growth of ginseng (Panax ginseng). This study investigated the effect of irrigation and ginseng root residue addition on the soil microbial community and root rot disease in 2-year-old ginseng. Methods and Results: Each $20{\ell}$ pot was filled with soil infected with ginseng root rot pathogens, and irrigated daily with $2{\ell}$ of water for one month. After the irrigation treatment, ginseng fine root powder was mixed with the irrigated soil at a rate of 20 g per pot. In descending order, ${NO_3}^-$, electric conductivity (EC), exchangeable Na (Ex. Na) and K (Ex. K) decreased due to irrigation. In descending order, ${NO_3}^-$, EC, Ex. K, and available $P_2O_5$ increased with the additon of ginseng powder to the soil. The abundance of Trichoderma crassum decreased with irrigation, but increased again with the incorporation of ginseng powder. The abundance of Haematonectria haematococca increased with irrigation, but decreased with the incorporation of ginseng powder. The abundance of Cylindrocarpon spp. and Fusarium spp., which cause ginseng root rot, increased with the incorporation of ginseng powder. The abundance of Arthrobacter oryzae and Streptomyces lavendulae increased with irrigation. The abundance of Streptomyces lavendulae decreased, and that of Arthrobacter spp. increased, with the incorporation of ginseng powder. Aerial growth of ginseng was promoted by irrigation, and ginseng root rot increased with the incorporation of ginseng powder. Conclusions: Ginseng root residues in the soil affected soil nutrients and microorganisms, and promoted ginseng root rot, but did not affect the aerial growth of ginseng.

Types and Historical Transition of Korean Traditional Seokgasan (한국 전통 석가산의 유형과 역사적 변천)

  • Yoon, Young-Jo;Yoon, Young-Hwal
    • Journal of the Korean Institute of Landscape Architecture
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    • v.38 no.3
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    • pp.83-97
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    • 2010
  • Seokgasan which is artificial rock mountain had been developed into one of the axes of traditional rock landscape of Goryo period and Joseon period as the central component of Korean traditional landscaping, but the legacy was cut off at the present. In the middle of the Goryo period, the Seokgasan made by piling rocks which were started from replicating miniature landscape has been developed into presenting the symbol and Seokgasan so it faced the new transition period of traditional rock garden culture. Seokgasan so it as small knoll, the Korean traditional Seokgasan was made differently the the surrounding landscape with big Seokgasan in China which overwhelms so it harmonize the surrounding landscape to build Korean style of the Seokgasan. This study is the objective investigation based on the old literature and the field remains, so it aims to so it comprehend the type of Seokgasan and developmental forms. At the result of investigation, 5 types of Seokgasan such as rocks piling on a pond, piling rocks, rocks in a pond, rocks on a ground, rock in a pot etc. has been developing in addition to the rocks which the oddly shaped rock is piled up as the basic framework. Among those, the piling rocks on a pond of Seokgasan which means water flows in Seokgasan is the representative Seokgasan which has been continued since the middle of the Goryo period until the end of the Joseon period. This study is expected to be the foundation which will succeed to legacy of Seokgasan tradition which was cut off and to develop by recovering historical landscaping value and identity of Seokgasan.

Insecticidal Activity of Essential Oils against Whitegrub (식물정유의 굼벵이에 대한 살충활성)

  • Lee, Dong Gon;Jung, Young Hak;Choi, Dae Hong;Choi, Sung Hwan;Choo, Ho Yul;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.4 no.2
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    • pp.129-134
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    • 2015
  • White grub damages various crops, trees and they can also be one of the most serious pests of turf grass in golf courses. This study was conducted to determine the biocontrol of white grubs with 18 different kinds (anise, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, and turpentine) of plant essential oils in laboratory, green house and field. Anise oil (88.9%), linseed oil (100%), and tea-tree oil (88.9%) were highly effective among tested essential oils against 3rd instar of Bifurcanmala aulax in cap vial experiment. However, anise and linseed oils showed low mortality against $3^{rd}$ instar of Popillia japonica in pot greenhouse experiment. Efficacy of anise, linseed oil, and tea-tree was also different depending on target white grub in field trials. Correlated mortality showed 32.6% only in tea-tree oil treatment against pupae of Adoretus tenuimaculatus however, correlated mortality of anise, linseed and tea-tree oil were 54.8, 51.6 and 56.5% respectively against $3^{rd}$ instar of Exomala orientalis in the field trial in Adelscott Country Club in Hapcheon, Gyeongnam province.

Quality Characteristics of Fruit Spirits from a Copper Distillation Apparatus (동증류기를 이용한 과실증류주의 품질 특성)

  • Cho, Ho-Cheol;Kang, Soon Ah;Choi, Sung-Inn;Cheong, Chul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.5
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    • pp.743-752
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    • 2013
  • The objective of this study was to investigate the quality characteristics of spirits derived from fruit using copper distillation equipment. First, optimal yeast strains were chosen through a fermentation test on raw materials (apples, mandarins and rowanberries). The normal fermentation condition for rowanberries observed at a rowanberry concentration of 8% during mashing included chaptalization with sugar to increase the alcohol content. During the mashing, fruits were fermented and distilled through one of three different types of distillation apparatuses (pot distiller, vacuum distiller, or multistage distiller made of copper). The results revealed that the type of apparatus used affects the content of alcohol and flavor components. The distilled spirits prepared through a copper multistage distiller had a much higher tendency to retain components of fruit aromas such as ethyl acetate and provided higher yields than spirits prepared with a pot distiller or vacuum distiller. Thus, the copper multistage distiller apparatus can be applied to positively influence the taste and flavor of fruit distilled spirits by enhancing fruit aromas, removing impurities such as sulfur components and enhancing yields.

A Study on the Development of Poly-Urea for Porcelain Restoration (폴리우레아 도자기 복원 재료의 적용)

  • Han, Won-Sik;Wi, Koang-Chul;Oh, Seung-Jun;Lee, Young-Hoon
    • Journal of Conservation Science
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    • v.34 no.1
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    • pp.23-29
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    • 2018
  • This study synthesized poly-urea and used it as a filler material for the restoration of porcelain. The synthesized poly-urea was manufactured as a resin and hardener mix that does not undergo contraction during curing, and is unfading. Given an adhesion strength of $180kg/cm^2$ and shearing strength of $200kg/cm^2$, the synthesized poly-urea exhibited the same efficacy as the epoxy putty currently sold in the market. Moreover, it also overcame the drawback of foaming encountered by urethane restoratives, which are a structurally similar type. The hardening time and pot life could also be controlled using additives. The poly-urea used for the restoration of modern artifacts presented a pot life of approximately one hour and took 12 h for complete hardening ($T_{90}$). When a $2{\times}2{\times}2cm$-sized test sample was added to xylene, poly-urea started to separate approximately two hours later and completed perfect pulverization within the solution 24 h later, demonstrating its reversibility. When directly applied to contemporary artifacts, it demonstrated the potential for restoration, as well as convenience and colorfulness.

Enhancement of Biological Control of Botrytis cinerea on Cucumber by Foliar Sprays and Bed Potting Mixes of Trichoderma harzianum YC459 and Its Application on Tomato in the Greenhouse

  • Lee Sun-Kug;Sohn Hwang-Bae;Kim Geun-Gon;Chung Young-Ryun
    • The Plant Pathology Journal
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    • v.22 no.3
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    • pp.283-288
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    • 2006
  • Trichoderma harzianum YC459 (Th 459), isolated from sawdust compost, was effective in controlling cucumber and tomato gray mold caused by Botrytis cinerea under controlled and plastic film tunnel conditions. A water suspension of the wettable powder formulation of Th 459 significantly $(P\leq0.05)$ reduced the severity of cucumber gray mold by foliar spraying at all tested concentrations from $10^5\;to\;10^8$ colony forming unit (cfu)/ml in repeated experiments. The control efficacy was maintained at least seven days with the average control value of 70% in cucumber pot tests. Mixing one to eight grams of the granular formulation ($10^8cfu/g$ dry weight) of Th 459 into one liter nursery potting mix at seeding also significantly $(P\leq0.05)$ reduced the severity of cucumber gray mold by suppression of lesion formation three weeks after treatment. Application of mixing granular formulation at seeding in combination with foliar spraying during cultivation provided a more significant reduction $(P\leq0.05)$ of cucumber gray mold than granule mixing or leaf spray alone. The foliar spraying of the formulated wettable powder of Th 459 significantly $(P\leq0.05)$ reduced the infection of tomato fruits by B. cinerea as effective as the chemical fungicide, dichlofluanid, in three plastic film tunnel experiment trials. It is suggested that effective control of gray mold of cucumber and tomato can be provided by both treatment of Th 459 into potting mix and foliar spray through induction of systemic resistance and direct inhibition of the pathogen.