• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.70-70
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• 2003

This study was conducted to determine the effects of the dietary microbial phytase (P) supplementation on growth performance and bioavailability of phosphorus in juvenile olive flounder, Paralichthys olivaceus and juvenile Korean rockfish, Sebastes schlegeli fed soybean meal-based diets. Nine experimental diets were formulated to be isonitrogenous and isocalolic to contain 50.0%crude protein (CP) and 16.7kJ, 48.6% crude protein (CP) and 16.0kJ available energy/g without the dietary phytase supplementation for olive flounder and Korean rockfish, respectively : 100% fish meal (FM); 70% FM ＋ 30% soybean meal (SM); 70% FM ＋ 30% SM ＋ Phytase(P) 1000U/kg diet; 70% FM ＋ 30% SM ＋ P 2000U/kg diet; 70% FM ＋ 30% SM with phytase-treated (Ptre) P 1000U/kg diet; 60% FM ＋ 40% SM; 60% FM ＋ 40% SM ＋ P 1000U/kg diet; 60% FM ＋ 40% SM ＋ P 2000U/kg diet; 60% PM ＋ 40% SM with Ptre P 1000U/kg diet. After two weeks of the conditioning period, triplicate groups of 25 fish initially averaging 6.15$\pm$0.04g, was randomly distributed into the aquarium for olive flounder, and 20 fish initially averaging 7.25$\pm$0.04g was randomly distributed into the aquarium for Korean rockfish. After 8 weeks feeding trials, there was no significant difference on weight gain fish fed between 100% FM and 70% FM＋30%SM with Ptre P 1000U in olive flounder and Korean rockfish. Apparent phosphorus digestibility fish fed phytase supplemental diets were significantly higher than those of fish fed phytase non-supplemental diets in olive flounder and Korean rockfish. Therefore, these results indicated that pre-treated soybean meal with phytase 1000U could replace fishmeal up to 30% for the maximum growth of juvenile olive flounder and Korean rockfish. Phytase supplementation could improve apparent digestibility of phosphorus in olive flounder and Korean rockfish fed soybean meal based diets.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.532-539
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• 2004

For efficient lincomycin production from Streptomyces lincolnensis L1245, various vegetable oils, natural nitrogen sources, and surfactants were investigated at the pilot-scale level in the flask. Olive oil as the sole carbon source was the most suitable one for producing lincomycin. When 20 g/lof olive oil was used, the lincomycin concentration and lipase activity reached 1.01 g/land 182 U/ml, respectively, after 5 days of culture. Among the various unsaturated fatty acids, when linolenic acid was used, the cell growth and lincomycin production were markedly decreased. On the other hand, when 0.2 g/l of oleic acid was added to the culture broth, the maximum lincomycin concentration was 1.0 g/l, which was about 1.7-fold higher than that obtained without the addition of oleic acid. Among the various natural nitrogen sources, pharmamedia or soybean meal was the most suitable nitrogen source. In particular, in the case of a mixture of 10 g/l of pharmamedia and soybean meal, 1.5 g/l of lincomycin concentration and 220 U/ml of lipase activity were obtained. When Span 180 was used as the surfactant, lincomycin production, lipase activity, and oil consumption increased. The correlation between the consumption rates of oil and lincomycin production in a culture using olive oil as the sole carbon source was also investigated. The lincomycin production depended on the consumption rate of olive oil. Using these results, fed-batch cultures for comparing the use of olive oil and starch as a conventional carbon source were carried out in a 5-1 fermentor. When olive oil was used as the sole carbon source, 34 g/l of olive oil was consumed after 7 days of culture. The maximum lincomycin concentration was 3.0 g/l, which was about 2.0-fold higher than that of starch medium after 7 days of culture. The product yield was 0.09 gig of consumed carbon source, which was about 3.0-fold higher than that of starch medium after 7 days of culture.

• 한국관광식음료학회:학술대회논문집
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• 한국관광식음료학회 2002년도 학술논문발표회
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• pp.112-122
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• 2002

본 연구는 Egg yolk을 저온 세균(62.5$^{\circ}C$에서 4분)과 고온 세균(95$^{\circ}C$에서 30초) 하여 7$0^{\circ}C$-75$^{\circ}C$ Double bath pen에서 Hot sabayon cream으로 하여 Hot Clarified Olive oil을 서서히 첨가하며 Emulsify하여 유화한 다음 Extract spices 첨가하여 유화제품의 색도 차이를 분석하여 품질을 평가하였으며, 제조된 유화난황의 관능검사를 실시하였다. Extract spices는 A군과 B.군으로 하였으며, A.군은 Garlic을 제외한 Extract였고 B.군은 Garlic을 첨가하였다. 결과는 Figure 1-5.과 같이 나타났다. \circled1 Sabayon Cream 관능검사 결과는 노랑자위 향이 감소 되었다. \circled2 Clarified Olive 관능검사 결과는 olive oily 향이 감소되고 고소한 맛이 났다. \circled3 Sabayon과 Clarified olive oil과의 Emulsified 관능검사 결과는 부드러워 졌다. \circled4 Extract Spices A.시료의 Hot Emulsified 관능검사 결과는 oily 하였다. \circled5 Extract Spices B.시료의 Hot Emulsified 관능검사 결과는 마늘의 향이 전체적 향을 부드럽게 하였으며, 전체적인 맛은 결정적으로 우수하였다. 즉 마늘 향이 첨가된 B시료가 효과와 특성이 있었다.

• 한국축산식품학회지
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• 제37권6호
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• pp.865-872
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• 2017

The objective of this study was to determine the antioxidant and quality characteristics of yogurt added green olive powder stored at $4^{\circ}C$ for 15 d. The following four groups were used in this study: Control group (GY0), Yogurt added with 1% green olive (GY1), with 3% green olive (GY3), and with 5% green olive (GY5). The more time of titratable acidity went by, the more it increased. Except GY0, viscosity tended to decrease in other groups (p>0.05), and the more time of syneresis went by, the more it increased, but GY3 of them showed the lowest syneresis. Lactic acid bacteria showed no significant with GY0 until 5 d, but after that, GY1, GY3 and GY5 showed lower than GY0. Yogurt added green olive showed darker color than GY0 (low $L^*$ and high $a^*$). The antioxidant activity of GY5 was found to be the highest among the four groups at day 1 of storage. Total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and reducing power of GY5 was found to be the highest among the four groups at day 1 of storage which were 6.96 mg GAE/kg, 47.53%, and 0.57, respectively. In the sensory evaluation sweet and overall of GY3 indicated the highest score among the four groups. Results of this study demonstrated that green olive powder might be used to improve the antioxidant capacity and sensory characteristics of yogurt.

• 한국수산과학회지
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• 제53권4호
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• pp.628-636
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• 2020

Streptococcosis in the olive flounder Paralichthys olivaceus can be caused by Streptococcus parauberis. We compared three routes of administration for experimental injections of the S. parauberis 19FBSPa0003 strain in the olive flounder. Pathological changes were observed during the experimental infection. Inflammation of the serous membrane in the liver, intestine, spleen and heart was the major pathological change found in the infected olive flounder. No mortality was observed in fish that received intraperitoneal (IP) injection at less than 1×10⁴ colony-forming unit (CFU)/fish. The lethal dose 50 for olive flounder, given an intravenous (IV) injection, was 7.94×10⁴ CFU/fish. Fish with a higher concentration of IV injected S. parauberis (1×10⁸ CFU/fish) died within a maximum of two days. However, serious necrosis and bacterial proliferation in ellipsoidal cells of the spleen and heart tissues were found in moribund or dead fish, 1-2 days after IV injection. Similar histopathological signs were observed in olive flounder inoculated by subcutaneous (SC) infected and naturally infected. In addition, SC was also strongly associated with bacteria concentration and cumulative mortality rate. Based on these results, SC is the recommended method for artificial infection by S. parauberis in the olive flounder.

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.275-286
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• 2014

To successful molecular breeding, identification and functional characterization of breeding related genes and development of molecular breeding techniques using DNA markers are essential. Although the development of a useful marker is difficult in the aspect of time, cost and effort, many markers are being developed to be used in molecular breeding and developed markers have been used in many fields. Single nucleotide polymorphisms (SNPs) markers were widely used for genomic research and breeding, but has hardly been validated for screening functional genes in olive flounder. We identified single nucleotide polymorphisms (SNPs) from expressed sequence tag (EST) database in olive flounder; out of a total 4,327 ESTs, 693 contigs and 514 SNPs were detected in total EST, and these substitutions include 297 transitions and 217 transversions. As a result, 144 SNP markers were developed on the basis of 514 SNP to selection of useful gene region, and then applied to each of eight wild and culture olive flounder (total 16 samples). In our experimental result, only 32 markers had detected polymorphism in sample, also identified 21 transitions and 11 transversions, whereas indel was not detected in polymorphic SNPs. Heterozygosity of wild and cultured olive flounder using the 32 SNP markers is 0.34 and 0.29, respectively. In conclusion, we identified SNP and polymorphism in olive flounder using newly designed marker, it supports that developed markers are suitable for SNP detection and diversity analysis in olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

• Fisheries and Aquatic Sciences
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• 제10권1호
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• pp.1-7
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• 2007

The induction of cellular and humoral immunity and cytokine gene expression by synthetic CpG oligodexoynucleotides (CpG-ODNs) has not been investigated systematically in olive flounder Paralichthys olivaceus in vivo. We optimized the proper concentration of CpG-ODNs using an in vitro assay for the superoxide anion $(O_2^-)$. CpG-ODNs induced $O_2^-$ and nitric oxide (NO) production, lysozyme activity, and the proinflammatory cytokine gene expression of $IL-1{\beta}$ and $TNF-{\alpha}$ in olive flounder significantly in vivo, whereas non-CpG-ODNs did not produce these effects or produced them to a lesser extent. This implied that CpG-ODNs could stimulate cellular and humoral immunity and cytokine gene expression in olive flounder. This is the first evidence of NO production and the first study on the mRNA expression of the proinflammatory cytokine genes $IL-1{\beta}$ and $TNF-{\alpha}$ in olive flounder in response to CpG-ODNs. Comparison of the variation in NO production and lysozyme activity to that of other studies led us to postulate that a group-specific difference exists in the immune responses of olive flounder against CpG-ODNs. Furthermore, the detailed immunostimulatory spectrum of CpG-ODNs in olive flounder could be a useful index with which to analyze the effect of CpG-ODNs against the challenge test prior to field applications.

• 공업화학
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• 제29권3호
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• pp.342-349
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• 2018

본 연구에서는 올리브 오일의 에스터 교환반응으로 생성된 복합소재(olive oil esters)의 화장품 응용을 위해 물리화학적 성질, 유화력, 보습력 및 세포독성을 평가하였다. 실험에는 반응시간이 짧은 olive oil esters S와 반응시간이 긴 olive oil esters L을 사용하였다. 먼저 TLC 이미지 분석으로 조성비를 확인한 결과, olive oil esters S의 조성은 mono-, di-, tri-glyceride가 5.2, 24.1, 46.4%이었고, fatty acid ethyl ester는 21.9%이었다. Olive oil esters L은 mono-, di-, tri-glyceride가 4.1, 24.7, 40.6%이었고, fatty acid ethyl ester는 28.8%이었다. 올리브 오일 에스터를 화장품에 사용하기 위한 기준 및 시험방법 설정을 위해 요오드가, 산가, 검화가, 불검화물, 굴절률, 비중 및 순도시험을 수행하였고 표준화시켰다. 또한 올리브 오일 에스터의 유화력 평가로서, 계면활성제 없이 O/W 에멀전을 제조하여 유화 입자를 확인하였다. 올리브 오일 에스터를 사람 피부에 도포 5일 후, 피부 수분 보유량은 초기보다 13.1% 개선되었다. 피부 세포에 대한 독성을 평가한 결과, 올리브 오일 에스터는 $0.2-200{\mu}g/mL$에서 세포 생존율 90% 이상을 나타내 안전성이 확인되었다. 결과적으로 올리브 오일 에스터는 화장품 산업에서 천연/무독성 원료로서 응용가능성이 있음을 시사하였다.

• Fisheries and Aquatic Sciences
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• 제5권4호
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• pp.258-262
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• 2002

Effects of Escherichia coli genomic DNA on the production of phagocyte activating supernatants by the head kidney leucocytes isolated from olive flounder (Paralichthys olivaceus) were investigated. Phagocyte activating activity of the supernatants was estimated by. measuring reactive oxygen species (ROS) production in target head kidney phagocytes. All supernatants from olive flounder head kidney leucocytes-stimulated with E. coli DNA induced significantly (P<0.01) higher ROS production from target pagocytes than the unstimulated control supernatant. Maximum enhancement of chemiluminescent response was observed $5.0-10.0{\mu}g\;mL^{-1}$ of bacterial DNA while the increment ability was decreased significantly (P<0.01) at the concentration of $20.0{\mu}mL^{-1}$. The results demonstrate that olive flounder head-kidney leucocytes stimulated with bacterial DNA release a soluble phagocyte activating cytokines capable of enhancing the respiratory burst activity from target phagocytes.

• 한국수산과학회지
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• 제40권3호
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• pp.128-132
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• 2007

We identified a microsatellite marker, Poli121TUF, which appears to be significantly linked (P<0.001) with a lymphocystis disease virus (LCDV)-resistance gene in the olive flounder, Paralichthys olivaceus. The olive flounder is an economically important food fish, that is widely cultured in Korea, Japan, and China. Lymphocystis disease has spread in these countries and has seriously reduced the economic value of the fish. LCDV causes lymphocystis cells (LC) to form on the body surface, fins, gills, mouth, and intestine. Fish with LC lose commercial value due to their deformed appearance. The identified micro satellite marker can be used as a candidate locus for marker-assisted selection (MAS) in order to enhance the efficiency of selection for LCDV resistance in the olive flounder.