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http://dx.doi.org/10.5657/KFAS.2020.0628

An Experimental Study on Artificial Infection of Olive Flounder Paralichthys olivaceus by Streptococcus parauberis Using Different Injection Sites  

Kim, Tae-Ho (Pathology Division, National Institute of Fisheries Science)
Lee, Nam-Sil (Pathology Division, National Institute of Fisheries Science)
Choi, Hye-Sung (Pathology Division, National Institute of Fisheries Science)
Jung, Sung-Hee (Pathology Division, National Institute of Fisheries Science)
Han, Hyun-Ja (Pathology Division, National Institute of Fisheries Science)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.53, no.4, 2020 , pp. 628-636 More about this Journal
Abstract
Streptococcosis in the olive flounder Paralichthys olivaceus can be caused by Streptococcus parauberis. We compared three routes of administration for experimental injections of the S. parauberis 19FBSPa0003 strain in the olive flounder. Pathological changes were observed during the experimental infection. Inflammation of the serous membrane in the liver, intestine, spleen and heart was the major pathological change found in the infected olive flounder. No mortality was observed in fish that received intraperitoneal (IP) injection at less than 1×104 colony-forming unit (CFU)/fish. The lethal dose 50 for olive flounder, given an intravenous (IV) injection, was 7.94×104 CFU/fish. Fish with a higher concentration of IV injected S. parauberis (1×108 CFU/fish) died within a maximum of two days. However, serious necrosis and bacterial proliferation in ellipsoidal cells of the spleen and heart tissues were found in moribund or dead fish, 1-2 days after IV injection. Similar histopathological signs were observed in olive flounder inoculated by subcutaneous (SC) infected and naturally infected. In addition, SC was also strongly associated with bacteria concentration and cumulative mortality rate. Based on these results, SC is the recommended method for artificial infection by S. parauberis in the olive flounder.
Keywords
Olive flounder; Paralichthys olivaceus; Streptococcus parauberis; Subcutaneous injection; Artificial infection;
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1 Lee CH, Kim PY, Ko CS, Oh DC and Kang BJ. 2007. Biological characteristics of Streptococcus iniae and Streptococcus parauberis isolated from cultured flounder, Paralichthys olivaceus, In Jeju. J Fish Pathol 20, 33-40.
2 Morton DB, Jennings M, Buckwell A, Ewbank R, Godfrey C, Holgate B, Inglis I, James R, Page C, Sharman I, Verschoyle R, Westall L and Wilson AB. 2001. Refining procedures for the administration of substances. Lab Anim 35, 1-41.
3 Mori K, Fukuda Y, Togo A, Miyoshi Y and Endo M. 2010. Practical inoculation site for experimental infection of Japanese flounder Paralichthys olivaceus with Streptococcus parauberis. Gyobyo Kenkyu Fish Pathol 45, 37-42. https://doi.org/10.3147/jsfp.45.37.   DOI
4 Meyburgh CM, Bragg RR and Boucher CE. 2017. Lactococcus garvieae: an emerging bacterial pathogen of fish. Dis Aquat Organ 123, 67-79. https://doi.org/10.3354/dao03083.   DOI
5 Nho SW, Shin GW, Park SB, Jang HB, Cha IS, Ha MA, Kim YR, Park YK, Dalvi RS, Kang BJ, Joh SJ and Jung TS. 2009. Phenotypic characteristics of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder Paralichthys olivaceus. FEMS Microbiol Lett 293, 20-27. https://doi.org/10.1111/j.1574-6968.2009.01491.x.   DOI
6 Nho SW, Hikima JI, Cha IS, Park SB, Jang HB, del Castillo CS, Kondo H, Hirono I, Aoki T and Jung TS. 2011. Complete genome sequence and immunoproteomic analyses of the fish bacterial pathogen Streptococcus parauberis. J Bacteriol 193, 3356-3366. https://doi.org/10.1128/JB.00182-11.   DOI
7 Nam HJ, Park KI and Choi MS. 2014. Effects of propolis extracts on the immune response in cultured flounder, Paralichthys olivaceus. J Fish Pathol 27, 47-56. https://doi.org/10.7847/jfp.2014.27.1.047.   DOI
8 Randhawa MA. 2009. Calculation of LD50 values from the method of Miller and Tainter, 1944. J Ayub Med Coll Abbottabad 21, 184-185.
9 Domeenech A, Derenaaandez-Garayzabal JF, Pascual C, Garcia JA, Cutuli MT, Moreno MA, Collins MD and Dominguez L. 1996. Streptococcosis in cultured turbot, Scopthalmus maximus (L.), associated with Streptococcus parauberis. J Fish Dis 19, 33-38. https://doi.org/10.1111/j.1365-2761.1996.tb00117.x.   DOI
10 Eldar A, Ghittino C, Asanta L, Bozzetta E, Goria M, Prearo M and Bercovier H. 1996. Enterococcus seriolicida is a junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in fish. Curr Microbiol 32, 85-88. https://doi.org/10.1007/s002849900015.   DOI
11 Eldar A and Ghittino C. 1999. Lactococcus garvieae and Streptococcus iniae infections in rainbow trout Oncorhynchus mykiss: similar, but different diseases. Dis Aquat Org 36, 227-231. https://doi.org/10.3354/dao036227.   DOI
12 Fortier AH, Slayter MV, Ziemba R, Meltzer MS and Nacy CA. 1991. Live vaccine strain of Francisella tularensis: infection and immunity in mice. Infect Immun 59, 2922-2928.   DOI
13 Hoshina T. 1958. A Streptococcus pathogenic to fish. J Tokyo Univ Fish 44, 57-68.
14 Shim JD, Hwang DH, Jang SY, Kim TW and Jeong JM. 2019. Monitoring of the mortalities in olive flounder Paralichthys olivaceus farms of Korea. J Fish Pathol 32, 29-35. https://doi.org/10.7847/jfp.2019.32.1.029.   DOI
15 Heo MS, Song CB, Lee J, Yeo IK, Jeon YJ, Lee JJ, Chung SC, Lee KW, Rho S, Choi KS and Lee YD. 2001. Characteristics of ${\beta}$-Streptococcus spp. isolated in cultured flounder Paralichthys olivaceus of Jeju Island. J Korean Fish Soc 34, 365-369.
16 Heo JH, Jung MH, Cho MH, Kim GH, Lee KC, Kim JH and Jung TS. 2002. The epidemiological study on fish diseases in the Southern Area of Kyeognam. J Vet Clin 19, 14-18.
17 Han SY, Kang BK, Kang BJ, Shin SP, Soen BH, Kim JM, Kim JH, ChorescaJr CH, Han JE, Jun JW and Park SC. 2011. Prevalence and different characteristics of two serotypes of Streptococcus parauberis isolated from the farmed olive flounder Paralichthys olivaceus (Temminck and Schlegel), in Korea. J Fish Dis 34, 731-739. https://doi.org/10.1111/j.1365-2761.2011.01289.x.   DOI
18 Ringo E and Gatesoupe FJ. 1998. Lactic acid bacteria in fish: a review. Aquaculture 160, 177-203. https://doi.org/10.1016/S0044-8486(97)00299-8.   DOI
19 Seo HG, Do JW, Jung SH and Han HJ. 2016. Outbreak of hirame rhabdovirus infection in cultured spotted sea bass Lateolabrax maculatus on the western coast of Korea. J Fish Dis 39, 1239-1246. https://doi.org/10.1111/jfd.12513.   DOI
20 Toranzo AE, Devesa S, Heinen P, Riaza A, Nunez S and Barja JL. 1994. Streptococcosis in cultured turbot caused by an Enterococcus-like bacterium. Bull Eur Ass Fish Pathol 14, 19-19.
21 Woo SH, Kim HJ, Kim JW and Park SI. 2006. Pathogenicity and classification of streptococci isolated from cultured marine fished. J Fish Pathol 19, 17-33.
22 Won KM, Cho MY, Park MA, Kim KH, Park SI, Lee DC, Kwon MG and Kim JW. 2010. Pathological characteristics of olive flounder Paralichthys olivaceus experimentally infected with Streptococcus parauberis. Fish Sci 76, 991-998. https://doi.org/10.1007/s12562-010-0287-6.   DOI
23 Yoshida T, Endo M, Sakai M and Inglis V. 1997. A cell capsule with possible involvement in resistance to opsonophagocytosis in Enterococcus seriolicida isolated from yellowtail Seriola quinqueradiata. Dis Aquat Org 29, 233-235.   DOI
24 Choi HJ, Cho MY, Lee JI, Kwon MG, Choi DL, Kim JW, Han MC and Lee DC. 2009. The pathogenicity of Streptococcus parauberis isolated from cultured olive flounder Paralichthys olivaceus. J Fish Pathol 22, 263-273.
25 Yanong RP and Francis-Floyd R. 2002. Streptococcal infections of fish. Florida Cooperative Extension Service IFAS University of Florida, Florida, FL, U.S.A., 1-5.
26 Yolanda TC, Clara FA and Ysabel S. 2019. Proteomic and molecular fingerprinting for identification and tracking of fish pathogenic Streptococcus. Aquaculture 498, 322-334. https://doi.org/10.1016/j.aquaculture.2018.08.041.   DOI
27 Bennett B, Check IJ, Olsen MR and Hunter RL. 1992. A comparison of commercially available adjuvants for use in research. J Immunol Methods 153, 31-40. https://doi.org/10.1016/0022-1759(92)90302-A.   DOI
28 Baeck GW, Kim JH, Gomez DK and Park SC. 2006. Isolation and characterization of Streptococcus sp. from diseased flounder Paralichthys olivaceus in Jeju Island. J Vet Sci 7, 53-58. https://doi.org/10.4142/jvs.2006.7.1.53.   DOI
29 Chang PH, Lin CW and Lee YC. 2002. Lactococcus garvieae infection of cultured rainbow trout, Oncorhynchus mykiss, in Taiwan and associated biophysical characteristics and histopathology. Bull Eur Ass Fish Pathol 22, 319-327.
30 Cho MY, Kim MS, Kwon MG, Jee BY, Choi HS, Choi DL, Park GH, Lee CH, Kim JD, Lee JS, Oh YK, Lee DC, Park SH and Park MA. 2007. Epidemiological study of bacterial diseases of cultured olive flounder, Paralichthys olivaceus from 2005 to 2006 in Korea. J Fish Pathol 20, 61-70.
31 Kanai K, Yamada M, Fei M, Takahashi I, Nagano T, Kawakami H, Yamashita A, Matsuoka S, Fukuda Y, Miyoshi Y, Takami I, Nakano H, Hirae T, Shutou K and Honma T. 2009. Serological differentiation of Streptococcus parauberis strains isolated from cultured Japanese flounder in Japan. Fish Pathol 44, 33-39. https://doi.org/10.3147/jsfp.44.33.   DOI
32 Jeong YU, Kang CY, Kim MJ, Heo MS, Oh DC and Kang BJ. 2006. Characterization of Streptococcosis occurrence and molecular identification of the pathogens of cultured flounder in Jeju Island. Kor J Microbiol 42, 199-204.
33 Jee BY, Shin KW, Lee DW, Kim YJ and Lee MK. 2014. Monitoring of the mortalities and medications in the inland farms of olive flounder Paralichthys olivaceus, in South Korea. J Fish Pathol 27, 77-83. https://doi.org/10.7847/jfp.2014.27.1.077.   DOI
34 King JS. 1981. Streptococcus uberis: a review of its role as causative organism of bovine mastitis I. Characteristics of the organism. Br Vet J 137, 36-52. http://doi.org/10.1016/S0007-1935(17)31786-4.   DOI
35 Kim JH, Gomez DK, Baeck GW, Shin GW, Heo GJ, Jung TS and Park SC. 2006. Pathogenicity of Streptococcus parauberis to olive flounder Paralichthys olivaceus. Fish Pathol 41, 171-173. https://doi.org/10.3147/jsfp.41.171.   DOI
36 Kang CY, Kang BJ, Moon YG, Moon YG, Kim KY and Heo MS. 2007. Characterization of Streptococcus parauberis isolated from cultured olive flounder, Paralichthys olivaceus in the Jeju Island. J Fish Pathol 20, 109-117.
37 Kwon MG, Hwang JY, Seo JS and Jung SH. 2015. Effect of olive flounder Paralichthys olivaceus, immune genes stimulation by molecular adjuvant in vitro culture condition. JFMSE 27, 1470-1478. https://doi.org/10.13000/ JFMSE.2015.27.5.1470.   DOI
38 KOSIS (Korea Statistical Information Service). 2019. Investigation of fisheries aquaculture production. Retrieved from http://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1EZ0008&conn_path=I2 on Dec 10, 2019.