• Archives of Pharmacal Research
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• v.23 no.4
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• pp.418-423
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• 2000

Oka strain VR-795 (Varicella Zoster Virus, VZV) of American Type Culture Collection (ATCC) has been used for chickenpox vaccine production. In order to use this strain for vaccine production, the strain must be identified and its stability must be confirmed. The identification of the Oka strain has been confirmed using Restriction Fragment Length Polymorphism (RFLP) and DNA sequence analysis of glycoprotein-II (gp-II). The amino acid sequences of Oka deduced from the DNA sequence of gp-II have changed at three amino acids against Ellen and at one amino acid against Webster. To prove the stability of the Oka strain during the passage, RFLP and DNA sequence analyses were also used with 11, 15 and 23 times of virus passage. We found that the Oka strain was stable at passages of up to 23 times, based on the RFLP and DNA sequence analyses. The confirmed Oka strain was renamed as BR-Oka for the purposes of chickenpox vaccine production.

• Natural Product Sciences
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• v.5 no.2
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• pp.107-111
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• 1999

To investigate less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was isolated from the carpophores of Elfvingia applanata (pers.) Karst. Anti-varicella zoster virus (Oka strain; anti-VZV/Oka) activity of EA was examined in MRC-5 cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with nucleoside anti-VZV agents, acyclovir and vidarabine, were examined on the multiplication of VZV/Oka. EA exhibited a concentration-dependent reduction in the plaque formation of VZV/Oka with a 50% effective concentration $(EC_{50})$ of $464.14\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combination of EA with acyclovir showed more potent synergism with CI values of $0.18{\sim}0.62$ for $50{\sim}90%$ effective levels than that of EA with vidarabine with CI values of $0.67{\sim}1.04$.

• The Korean Journal of Mycology
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• v.27 no.3 s.90
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• pp.237-241
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• 1999

To search for less toxic antiviral agents from Basidiomycetes, the water soluble components (=EA), were isolated from the carpophores of Elfvingia applanata (Pers.) Karst. Anti-varicella zoster virus (Oka strain; anti-VZV/Oka) activity of EA was examined in MRC-5 cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or IFN gamma were examined on the multiplication of VZV/Oka. EA exhibited a dose-dependent reduction in the plaque formation of VZV/Oka with 50% effective concentration $(EC_{50})$ of $464.14\;{\mu}g/ml$. The results of the combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combination of EA with IFN alpha showed partially synergistic or additive effects with CI values of $0.83{\sim}1.09$ for 50%, 70%, 90% effective levels, and those with IFN gamma showed antagonism with CI values of $1.20{\sim}1.24$.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.11a
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• pp.327-334
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• 2000

응력-변형률 관계의 모델링에 있어서 creep, stress relaxation, strain rate effect 등의 묘사는 중요한 지반거동중의 하나인 시간 의존적 거동의 simulation은 있어서 대단히 중요한 요소라 할 수 있다. 특히 지반은 변형률 속도에 대하여 때로는 매우 다른 거동 특성을 보이기 때문에 지반의 모델링에 있어서 변형율 속도를 고려한 구성방정식의 제시는 큰 비중을 차지한다 하겠다. 본 연구에서는 변형율에 따라 변화하는 지반의 거동특성을 보다 현실에 가깝게 묘사하기 위한 구성모델을 제시하였다. 이를 위하여 Bounding Surface Model의 점탄소성 부분을 Perzyna(1966)와 Adachi and Oka(1982)의 구성방정식 이론을 이용하여 발전시켰다. 제안된 구성모델은 기존의 모델에 비하여 다양한 변형율 속도에 적용할 수 있는 모델 정수를 비교적 간단히 결정할 수 있다는 장점이 있으며, 변형율 속도의 영향뿐 아니라 creep, stress relaxation등의 현상도 잘 simulation 할 수 있다. 본 모델은 후에 엄격히 실시되는 실내시험을 통하여 검증될 예정이다.

• Proceedings of the Korean Geotechical Society Conference
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• 2001.03a
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• pp.207-214
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• 2001

응력-변형률 관계의 모델링에 있어서 creep, stress relaxation, strain rate effect 등의 묘사는 중요한 지반거동중의 하나인 시간 의존적 거동에 대한 simulation은 있어서 대단히 중요한 요소라 할 수 있다. 특히 지반은 변형률 속도에 대하여 때로는 매우 다른 거동 특성을 보이기 때문에 지반의 모델링에 있어서 변형율 속도를 고려한 구성방정식의 제시는 큰 비중을 차지한다 하겠다. 본 연구에서는 변형율에 따라 변화하는 지반의 거동특성을 보다 현실에 가갈게 묘사하기 위한 시간 의존적 구성모델을 제시하는데 있다. Bounding Surface Model의 Stress Invariant 부분을 Perzyna(1966)와 Adachi and Oka(1982)의 변형율 속도 의존적인 구성관계 이론을 이용하여 발전시켰다 제안된 구성모델은 다양한 변형율 속도에 적용에 있어서 기존의 방식보다 간단히 모델 정수들을 결정 할 수 있다. 지반거동의 수치적인 해석을 위하여 기존의 Bounding Surface Model에 사용되었던 Program Code를 발전 시켜 사용하였으며, 엄격히 시행된 실내시험의 결과와 비교/검증하였다.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.187-190
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• 2003

In order to apply a cyclic elasto-plastic and a viscoelastic-viscoplastic constitutive model to actual multi-layered ground conditions during large earthquake, numerica simulations were performed by a liquefaction analysis in the present study. From the liquefaction analysis, it was verified that the models can give a good description of the damping characteristics and liquefaction phenomena of ground accurately during large event which induces plastic deformation in large strain range.

• Journal of Convergence for Information Technology
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• v.8 no.6
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• pp.201-207
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• 2018

A genome contains all genetic information of an organism. Within a specific species, unique traits appear for each individual, which can be identified by analyzing nucleotide sequences. Many Genome-Wide Associations Studies have been carried out to find genetic associations and cause of diseases from slightly different base among the individuals. It is important to identify occurrence of slight variations for polymorphisms of individuals. In this paper, we introduce an analysis and visualization tool for specific variation pattern identification of polymorphisms in nucleotide sequences and show the validity of the tool by applying it to analyzing nucleotide sequences of subcultured pOka strain of varicella-zoster virus. The tool is expected to help efficiently explore allele frequency variations and genetic factors within a species.

• Pediatric Infection and Vaccine
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• v.7 no.1
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• pp.129-135
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• 2000

Purpose : We previously reported the short-term immunogenecity and safety of 47 passaged Oka strain live attenuated varicella vaccine in healthy children in 1997. Now, we conducted this two-year follow-up study to confirm the maintenance of immunity, the occurrence of natural varicella infection and the activation of vaccine induced latent infection on the same vaccine. Methods : 99 children who had been immunized by 47 passaged Oka strain live attenuated varicella vaccine in 1997 were followed up by questionnaire, and 46 children out of study group were followed up serologically. They were asked to report any instance of varicella or herpes zoster since they had been immunized. If there was any evidence of varicella or herpes zoster, they should be clinically or serologically confirmed by doctor. Also, those patients' parents were asked to report any instance of varicella or herpes zoster in their family, playmate, kindergarten, school, or other settings. The immunity to VZV was confirmed by EIA and FAMA test. Results : 6 recipients developed breakthrough varicella after exposure to VZV in family, kindergarten and school during follow-up period. However, clinical features of those patients were very mild and self limited without therapy. And none of the recipients developed herpes zoster during this observation period. The results of EIA test showed that study subjects were all seropositive except one, and the antibody titers and GMT of FAMA test were seropositively maintained in all subjects. Statistically, the antibody titers of EIA and FAMA test confirmed two years after vaccination were higher than those results confirmed one month after vaccination. Conclusion : Our study results suggest that the immunity of 47 passaged Oka strain live attenuated varicella is well maintained until 2 years later after vaccination, and mild natural infection after exposure to VZV can be occurred with low rate. There were not developing zoster in study vaccine after vaccination for two-years.

• Pediatric Infection and Vaccine
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• v.4 no.2
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• pp.257-264
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• 1997

Purpose: We performed this study to find out short period humoral immunogenicity and safety of 47 passaged Oka strain live attenuated varicella vaccine(1,400PFU) in 12 months to 15 years aged healthy children. Methods: Ninety nine healthy chidren, who have no histories of varicella vaccination, recent chicken pox illness and contact, allergy to other vaccines and underlying severe diseases, were involved in this study from April 1997 to August 1997. 5ml blood were collected before vaccination and after vaccination from all vaccinees to measure varicella membrane antibody by FAMA, and varicella IgG antibody by EIA. And immediate reactions within 30 minutes after vaccination, local and systemic reactions within 3 days after vaccination and vaccine induced systemic illness during 6 weeks postvaccination period were observed in all vaccinees to identify side effects of study vaccine. Results: 1) 49 seronegative and 50 seropositive vaccinees were identified in both prevaccination serologic tests. 2) Serologic responses after vaccination measured by the FAMA in seronegative group showed that the mean GMT level revealed 64.0, and seroconversion rate was 97.9%. And serologic responses after vaccination measured by the FAMA in seropositive group showed that the mean GMT level(242.2) was markedly elevated comparing with the mean GMT level(9.2) of pre vaccination. 3) The results of EIA in seronegative group revealed that postvaccination mean GMT was 435.2(prevaccination GMT; 78.7), and 100% seroconversion rate. Also, the results of EIA in seropositve group showed that the mean GMT level(769.9) of postvaccination was almostly two fold hihger than the mean GMT level(419.7) of prevaccination. 4) Observed local reactions like injection sites redness, pain, hardness and itching sense were mild and disappeared within 3 days, also shorterm systemic reactions like irritability, lethargy, poor appetites and rash were not remarkable. And there were no remarkable side effects due to vaccine during study period in all vaccinees. Conclusion: We confirmed that 47 paasaged Oka strain live attenuated varicella vaccine has high shorterm humoral immunogenicity and safety. However, we need more detail and longterm humoral and cell mediated immunogenicity studies of this vaccine including clinical field trials.

• Geomechanics and Engineering
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• v.3 no.3
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• pp.233-254
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• 2011

The consolidation behavior of Sri Lankan peaty clay is analyzed using an elasto-viscoplastic model. The model can describe the secondary compression behavior as a continuous process and it can also account for the effect of structural degradation on the consolidation analysis. The analysis takes into account all the main features involved in the process of peat consolidation, namely, finite strain, variable permeability, and the secondary compression. The material parameters required for the analysis and the procedures to evaluate them, using both standard laboratory and field tests, are explained. Initially, the model performance is assessed by comparing the predicted and the observed peat consolidation behavior under laboratory conditions. The results indicate that the model is capable of predicting the observed creep settlements and the effect of layer thickness on the settlement analysis of peaty clay. Then, the model is applied to predict the consolidation behavior of peaty clay under different field conditions. In this context, firstly, the one-dimensional field consolidation of peaty clay, brought about by the construction of compacted earth fill, is predicted. Then, the two-dimensional peat foundation response upon embankment loading is simulated. A good agreement is seen in the comparison of the predicted results with the field observations.