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Identification and in silico analysis of two types of serpin genes from expressed sequence tags (ESTs) of the Oriental land snail, Nesiohelix samarangae (동양달팽이 (Nesiohelix samarangae) 의 expressed sequence tags (ESTs) 로부터 분리한 2종류의 Serpin 유전자 분석)

  • Park, So Young;Jeong, Ji Eun;Hwang, Hee Ju;Wang, Tae Hun;Park, Eun Bi;Kim, Yong Min;Lee, Jun-Sang;Han, Yeon Soo;Yang, Seung-Ha;Lee, Yong Seok
    • The Korean Journal of Malacology
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    • v.30 no.2
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    • pp.155-163
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    • 2014
  • Serpins are a group of proteins involved in the regulation of serine and other type of proteases, and have been identified in many kinds of organisms from invertebrates to vertebrates. Serpins are known to regulate the proteolytic cascades of the innate immune pathways in addition to their roles in blood coagulation, angiogenesis, fibrinolysis, inflammation and tumor suppression. In this study, we have isolated two partial serpin gene fragments from expressed sequence tags (ESTs) of Nesiohelix samarangae. Dotplot analysis indicates that they are of two different types, Ns-serpin type 1 and Ns-serpin type 2. Ns-serpin type 1 has 819 bp coding region (272 amino acids), whereas Ns-serpin type 2 has 555 bp coding region (185 amino acids). Molecular phylogenetic analysis shows that the identified serpins have high similarities to their counterparts in the California see slug, Aplysia californica. Yet, the precise biological and immunological roles of these Ns-serpins remain to be further investigated using RNA interference and other molecular techniques.

Germination and Emergence of Major Upland Weeds II. Effects of Soil Depth, pH and Fertilization on Emergence of Weeds (주요(主要) 밭잡초(雜草) 종자(種子)의 발아(發芽) 및 출아(出芽)에 관(關)한 연구(硏究) II. 복토(覆土) 심도(深度), 산도(酸度), 시비(施肥)가 잡초종자(雜草種子)의 출아(出芽)에 미치는 영향(影響))

  • Woo, I.S.
    • Korean Journal of Weed Science
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    • v.11 no.3
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    • pp.224-228
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    • 1991
  • This study was carried to know factors affecting emergence of major upland weeds in soil in order to get basic information on weed control methods. Firthy eight percent of weed seeds were distributed within soil surface to 10cm in soil and 2% of weed seeds were observed in 40~50cm soil layer in field. As planting depth was deeper, emergence of weeds became poor. However Capsella bursa-pastoris can emerge at soil surface. Amaranthus retroflexus, Amaranthus lividus, Porturaca oleracea, Chenopodium album, Solanum nigrum upto 3cm, Eleusine indica, Echinochloa crus-galli, Setaria viridis, Digitaria sanguinalis upto 7cm. Emergence of weeds was very poor in very acid soil but good in soil pH 5.5~6.0. However emergence of weed was not affected by pH 3.5~4.0 or above. Emergence of E. indica, C. bursa-pastoris, A. retroflexus, A. lividus, C. album, E. crus-galli, Solanum nigrum, and S. viridis was good in loam soil and P. oleranea, D. sanguinalis in sandy loam soil. Emergence of weed seeds was not affected by fertilization.

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A study on the effects of exhaust emission standards on the required ventilation rate in vehicle tunnels (차량 배출가스 규제기준이 소요환기량에 미치는 연구)

  • Kim, Hyo-Gyu;Ryu, Ji-Oh;Song, Seog-Hun;Jung, Chang-Hoon
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.19 no.3
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    • pp.409-420
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    • 2017
  • The amount of ventilation required in making the tunnel ventilation plan is an important factor for determining the capacity of the ventilation system. The amount of pollutant emission for each type of vehicle (basic emission amount for the design of ventilation volume) for estimating the required ventilation amount is based on the 'Standard for Allowing the Emission for the car manufacturing', proposed by Ministry of Environment. However, in 2013, the Ministry of Environment announced the 'Regulations on the calculation method of total emissions from vehicles' as a regulation for calculating the pollutants emitted from vehicles. In this regulation, there are the 'Emission factors for each type of vehicle'. Therefore, it is necessary to review the application of the Regulation to the estimation of the required ventilation volume for the road tunnel. In this study, the influence of the strengthened emission regulation in 2015 caused by the case of manipulation of emission volume for the diesel vehicle on the calculation of the required ventilation volume in the road tunnel has been checked. In addition, in this study, the required ventilation volume calculated according to the Standard for Allowing the Emission for the car manufacturing revised by Ministry of Environment and "Emission factors for each type of vehicle" and that calculated according to the EURO emission standard were compared for analysis. This study has implications that it provides the basic design data for calculating the reasonable ventilation capacity of the ventilation system based on the ground for calculating the required ventilation volume.

Physiological Responses of Bupleurum latissimum Nakai, Endangered Plants to Changes in Light Environment (광환경조절에 따른 멸종위기식물 섬시호의 생리적 반응)

  • Lee, Kyeong-Cheol;Wang, Myeong-Hyeon;Song, Jae Mo
    • Journal of Bio-Environment Control
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    • v.22 no.2
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    • pp.154-161
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    • 2013
  • This study was conducted to investigate the physiological responses of Bupleurum latissimum, endangered plants by light condition. We investigated photosynthetic parameters, chlorophyll contents and chlorophyll fluorescence under different shading treatments (Shaded 50%, 70%, 90% and non-treated). Results showed that net apparent quantum yield (AQY) and chlorophyll contents were significantly increased with elevating shading level. However, light compensation point (LCP) and dark respiration ($R_d$) were shown the opposite trend. Especially, non-treated exhibited photoinhibition such as reduction of chlorophyll contents and maximum photosynthesis rate ($Pn_{max}$) also variation trend of stomatal conductance ($g_s$), and transpiration rate (E) were decreased to prevent water loss. Photosynthetic rate ($P_{Nmax}$) and photochemical efficiency (Fv/Fm) of 90% treatment showed a drastic reduction in July. This implies that photosynthetic activity will be sharply decreased with a long period of low light intensity. The 50% treatment showed relatively higher photosynthetic activity than other treated. This result suggested that growth and physiology of B. latissimum adapted to 50% of full sunlight.

Preparation of Trinitrophenyl Cellulose as Substrate for Cellulase Assay (Trinitrophenyl Cellulose의 조제)

  • Maeng, Jeong-Seob;Nam, Yoon-Kyu;Park, Seung-Heui;Choi, Woo-Young
    • Korean Journal of Agricultural Science
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    • v.22 no.2
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    • pp.151-155
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    • 1995
  • Two types of modified celluloses which contain trinitrophenyl groups as chromophore were synthesized from carboxymethyl cellulose Whatman CM 70 and CM 32. Diaminoethyl groups were added to the CM 70 and CM 32 to make DAE-CM celluloses and then the DAE-CM groups were substituted by 2,4,6-trinitrophenyl groups to produce TNP-celluloses. Average particle size of the TNP-cellulose from CM 32 was $44.6{\pm}9.6{\mu}m$ in diameter and $127.9{\pm}22.5{\mu}m$ in length, which was much smaller than those from CM 70, however its TNP-moiety per gram determined by using the molar extinction coefficient $1.33{\times}10^4$ of ${\varepsilon}$-TNP-lysine at 345 nm, was 0.68 millimoles, which was 5.6-fold greater than those from CM 70. The absorption spectrum of TNP-oligosaccharides which were the soluble products of TNP-celluloses by a cellulase preparation Onozuka R-10, showed a maximal peak at 344 nm. Increases in the absorbance during hydrolysis were linear with the enzyme concentration, and the differences of slope values between two types of TNP-celluloses that the more semsitive assay could be achieved by using those from CM 32 as substrate at the low range of the enzyme concentration.

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Changes of Qualities in Vacuum Packed Fermented Pork Using a Korean Traditional Sensoning During Storage (전통양념으로 발효숙성하여 진공포장한 돼지고기의 저장 중 품질 변화)

  • Jin, S.K.;Kim, I.S.;Hah, K.H.;Hur, S.J.;Park, K.H.;Lyou, H.J.;Bae, D.S.
    • Journal of Animal Science and Technology
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    • v.47 no.1
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    • pp.39-48
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    • 2005
  • Outside muscle of pork ham were cut to cube(7 $\times$ 10 $\times$2 ern) and three Korea traditional seasonings such as soybean paste(Tl), garlic paste(T2), red pepper paste(T3) were seasoned by the proportions of meat to seasonings(1 : 1), respectively. The seasoned samples were fermented by fill into plastic box at 0 $\pm$ 1 $^{\circ}C$ for 10 days. And then, the fermented meat from each pack was vacuum-packaged and stored at 0 $\pm$ 1 $^{\circ}C$ for up to 9 weeks. pH and shear force were decreased during storage periods in all treatment groups and WHC was decreased with storage in T2. The saccarinity of T1 was increased and salinity increased during storage in all treatment groups. pH of T2 was increased than that of other treatments, while decreased saccarinity and shear force of in T2. The salinity were higher in the order of T1 > T2 > T3. Volatile basic nitrogen (VBN) value were increased with storage in all treatment groups. Thiobarbituric acid reactive substances (TSARS) value of Tl was increased with storage while it was decreased T2. Thiobarbituric acid reactive substances(TSARS) value was higher in the order of T1 > T3 > T2 at 9weeks of storage. Surface meat L' values of T1 was increased with storage and T3 decreased with storage whereas, surface meat a' values of T1 was decreased with storage, and T2 was increased with storage. Surface meat b' values of T3 was decreased with storage. Escherichia coli were decreased during storage periods in all treatment groups.

Structural Capacity Evaluation of System Scaffolding using X-Type Advanced Guardrail (교차가새형 선행 안전난간을 적용한 시스템비계의 구조 성능 평가)

  • Park, J.D.;Lee, H.S.;Shin, W.S.;Kwon, Y.J.;Park, S.E.;Yang, S.S.;Jung, K.
    • Journal of the Korean Society of Safety
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    • v.35 no.5
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    • pp.49-58
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    • 2020
  • In domestic construction sites, when installing steel pipe scaffolding and system scaffolding, the guardrails are installed after the installation of the work platforms. This conventional guardrail system (CGS) is always exposed to the risk of falls because the safety railing is installed later. In order to prevent fall disasters during erecting and dismantling scaffolds, it is necessary to introduce the advanced guardrail system (AGS) which installs railings in advance of climbing onto a work platform. For the introduction of the AGS, the structural performance of the system scaffolding applying the CGS and the AGS was compared and evaluated. The structural analysis of the system scaffold (height: 31 m and width: 27.4 m) with AGS confirmed that structural safety was ensured because the maximum stress of each element of the system scaffolding satisfies the allowable stress of each element. As a result of performance comparison of CGS and AGS for each element, the combined stress ratio of vertical posts in AGS was 6.4% lower than that of CGS. In addition, in the case of ledger and transom, the combined stress ratios of AGS and CGS were almost the same. The compression test of the assembled system scaffolding (three-storied, 1 bay) showed that the AGS had better performance than the CGS by 9.7% (8.91 kN). The cross bracing exceeds the limit on slenderness ratio of codes for structural steel design. But the safety factor for the compressive load of the cross bracing was evaluated as meeting the design criteria by securing 3 or more. In actual experiments, it was confirmed that brace buckling did not occur even though the overall scaffold was buckled. Therefore, in the case of temporary structures, it was proposed to revise the standards for limiting on slenderness ratio of secondary or auxiliary elements to recommendations. This study can be used as basic data for the introduction of AGS for installing guardrails in advance at domestic construction sites.

Two unrecorded naturalized plants in Korea: Stachys agraria and Dracocephalum moldavica (Lamiaceae) (한반도 미기록 귀화식물: 애기석잠풀과 향용머리)

  • Jeong, Keum Seon;Jang, Chang-Seok;Park, Soo Hyun;Lee, Jung Sim;Yoon, Suk Min;Kim, Tae Hwan;Shin, Chang Ho;Choi, Kyung
    • Korean Journal of Plant Taxonomy
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    • v.46 no.4
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    • pp.413-419
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    • 2016
  • Two unrecorded naturalized species, Stachys agraria Schltdl. & Cham. and Dracocephalum moldavica L (Lamiaceae), are newly reported in Korea. Stachys agraria and D. moldavica were found at Seogwipo-si on Jeju-do and at ChunChen-si in Gangwondo in Korea, respectively. Stachys agraria is distinguished from other related Korean taxa by corolla of 2.4-5.2 mm long with six to twelve flowers in the node. Its local name is "Ae-gi-seok-jam-pul" based on shorter than longer corollar. Dracocephalum moldavica was distinguishable from other Dracocephalum in Korea by the following combination of characters, oblong to ovate-triangular ($1.7-2.4cm{\times}0.8-1.2cm$) leaves and rough crenate at the margin. This taxon is referred to as 'Hyang-yong-meo-ri' in Korean based on its fragrance. We provide a description, illustrations, photographs, and a key of related taxa in Korea.

Manufacturing Techniques of Bronze Medium Mortars(Jungwangu, 中碗口) in Joseon Dynasty (조선시대 중완구의 제작 기술)

  • Huh, Ilkwon;Kim, Haesol
    • Conservation Science in Museum
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    • v.26
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    • pp.161-182
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    • 2021
  • A jungwangu, a type of medium-sized mortar, is a firearm with a barrel and a bowl-shaped projectileloading component. A bigyeokjincheonroe (bombshell) or a danseok (stone ball) could be used as a projectile. According to the Hwaposik eonhae (Korean Translation of the Method of Production and Use of Artillery, 1635) by Yi Seo, mortars were classified into four types according to its size: large, medium, small, or extra-small. A total of three mortars from the Joseon period have survived, including one large mortar (Treasure No. 857) and two medium versions (Treasure Nos. 858 and 859). In this study, the production method for medium mortars was investigated based on scientific analysis of the two extant medium mortars, respectively housed in the Jinju National Museum (Treasure No. 858) and the Korea Naval Academy Museum (Treasure No. 859). Since only two medium mortars remain in Korea, detailed specifications were compared between them based on precise 3D scanning information of the items, and the measurements were compared with the figures in relevant records from the period. According to the investigation, the two mortars showed only a minute difference in overall size but their weight differed by 5,507 grams. In particular, the location of the wick hole and the length of the handle were distinct. The extant medium mortars are highly similar to the specifications listed in the Hwaposik eonhae. The composition of the medium mortars was analyzed and compared with other bronze gunpowder weapons. The surface composition analysis showed that the medium mortars were made of a ternary alloy of Cu-Sn-Pb with average respective proportions of (wt%) 85.24, 10.16, and 2.98. The material composition of the medium mortars was very similar to the average composition of the small gun from the Joseon period analyzed in previous research. It also showed a similarity with that of bronze gun-metal from medieval Europe. The casting technique was investigated based on a casting defect on the surface and the CT image. Judging by the mold line on the side, it appears that they were made in a piece-mold wherein the mold was halved and using a vertical design with molten metal poured through the end of the chamber and the muzzle was at the bottom. Chaplets, an auxiliary device that fixed the mold and the core to the barrel wall, were identified, which may have been applied to maintain the uniformity of the barrel wall. While the two medium mortars (Treasure Nos. 858 and 859) are highly similar to each other in appearance, considering the difference in the arrangement of the chaplets between the two items it is likely that a different mold design was used for each item.

A Case Study of Successful Strategy for Farm's Franchise Commercialization through Local Agricultural Products - Focusing on the case of Jung Donuts Co. Ltd., in Yeongju, Gyeongbuk - (지역농산물을 이용한 농촌프랜차이즈 사업화 성공 사례연구 - 경북 영주시 (주)정도너츠 사례를 중심으로 -)

  • Seo, Min-gyo;Hwang, Bo-Jun;Song, Ji-Hyeon
    • The Korean Journal of Franchise Management
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    • v.4 no.1
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    • pp.1-24
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    • 2013
  • The purpose of this work is to analyze the case that Yeongju Municipality Government of Gyeongbuk and Jung Donuts Co., Ltd., a franchise company, activated local economy through Farm's Franchise Commercialization (FCC) and to establish the concept of FCC. As a food service franchise company, Jung Donuts specializes in making and selling glutinous rice donuts. In cooperation with Commodity Dept. of Yeongju Municipality Government, the company cultivates raw materials under contract. Such farm's franchise business has the following advantages: First, it is meaningful in the point that it contributes to opening a new market of local agricultural products in a stable way. In short, by using most agricultural products of Yeongju as raw materials, the business serves as a stable market for farmers. Secondly, it is possible to set a reasonable price through stable supply of raw materials. It is to advantages of both producers and consumers. Thirdly, the business brings about the effect of employment rise. It can cause increases in employment of franchise head office and its agencies, and of the Commodity Dept.. Lastly, it produces the promotion effect of local special products. By expanding its agencies across the country, a franchise business can promote the items of raw materials in terms of marketing. The successful FCC needs to meet three requirements as follows. The first one is to establish systematic logistic system. Stable logistic system is required in order to directly distribute and deliver products to nationwide agencies by a producing place. The second one is constant R&D activity. Through the activity of R&D of raw materials and equipment, they should be used most effectively. The third one is to build mutual trust relationship. For long-term business achievements, it is required to establish mutual trust relationship in which relevant entities share their visions with each other and cooperate with each other.