• Title/Summary/Keyword: Oak Leaves

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Inhibitory effects of the extract from Quercus dentata gallnut against plant virus infection

  • Kwon, S.B.;Shin, J.E.;Ahn, S.Y.;Yoon, C.S.;Kim, B.S.
    • Korean Journal of Organic Agriculture
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    • v.19 no.spc
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    • pp.271-274
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    • 2011
  • Pepper mild mosaic virus (PMMoV) and cucumber mosaic virus (CMV) are important pathogens in various vegetable crops worldwide. We have found that methanol extracts of Quercus dentate (Oaimyo Oak) gal/nut strongly inhibit PMMoV and CMV infection. Based on this result, the inhibitor named as "KN0912" formulated from the extract of Q. dentate gallnut was tested for its inhibitory effects on PMMoV or CMV infection to each local lesion host plant (Nicotiana glutinosa; PMMoV, Chenopodium amaranticolor; CMV). Pre-treatment effect of KN0912 against infections of each virus to local host plant was measured to be $75.1{\pm}0.5{\sim}97.5{\pm}1.5%$ to PMMoV and $70.6{\pm}2.2{\sim}99.0{\pm}1.0%$ to CMV in 1~10mg/ml conc. and the absorption effect of the antiviral composition of KN0912 to the inside of tobacco leaves tissue, was inhibited by 55.7% to PMMoV and 63.8% to CMV. The persistence of KN0912 treatment was maintained until after the 3 days high inhibitory effect by 98% to PMMoV and by 95.1% to CMV. Inhibitory effects on systemic host plants of KN0912 were measured to be 80~90% to PMMoV and 60~75% to CMV. From the change of morphological characteristics of PMMoV particles under EM, we are tentatively suggested that one mode of action of KN0912 is inactivation due to the destruction of virus particles.

Development of Analytical Method and Monitoring for Bifenazate in Commercial Agricultural Products (농산물 중 Bifenazate의 분석법 개선 및 모니터링)

  • Park, Eun-Heui;Go, Myoung-Jin;Cho, Myong-Shik;Kim, Young-Sun;Lee, Jin-Ha;Choi, Dong-Mi
    • The Korean Journal of Pesticide Science
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    • v.14 no.1
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    • pp.21-29
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    • 2010
  • The analytical method for bifenazate was developed using a HPLC (UVD). Also, analytical condition of LC/MS was set up for bifenazate. We validated the method for the precision and the reproducibility. The correlation coefficient of bifenazate ranged from 0.05 to 2.5 mg/kg was 1.0. Limit of quantitation (LOQ) was 0.01 mg/kg. To measure recoveries from agricultural products such as foxtail millet (cereal grains), kidney bean (beans), orange (fruits), perilla leaves (vegetables) and oak mushroom (mushrooms), bifenazate was spiked. Mean recoveries of bifenazate for each sample were 82.7~104.1% at the level of 0.1 mg/kg and 73.1~104.3% at the level of 0.5 mg/kg. The relative standard deviations (n=3) were 0.2~9.7%. Pesticide residues for bifenazate were investigated in 16 commodities (rice, foxtail millet, buckwheat, kidney bean, peanut, sesame, orange, grapefruit, kiwifruit, spinach, perilla leaves, leek, garlic stem, garlic, ginger and oak mushroom) collected from 22 provinces in 2009. Bifenazate was analyzed using analytical method by HPLC from 304 samples, and residue was not detected.

A Demonstrative Study on the Intake Habits of Dairy Goats (Saanen) Fed with Roughages (유산양의 조사료 채식습성에 관한 실증적 연구)

  • Gang, Byung-Ho;Lee, In-Duk;Lee, Hyung-Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.1
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    • pp.63-72
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    • 2009
  • The experiment was conducted from 2007 to 2008. The experimental roughages include five sources and 25 species in all; grasses and legumes: 5 species (mixed grasses, orchardgrass, tall fescue, alfalfa, white clover), native grasses and weeds: 5 species (mixed native grasses, Miscanthus sinensis Anderss, Arundinella hirta (Thunb.) Tanaka, barnyard grass, short awn, forage crops and straw: 5 species (barley 4- hairy vetch, wheat 4- hairy vetch, rye silage, barley silage, baled rice straw), browse and fallen leaves: 5 species (mixed browse, oriental white oak browse, Quercus serrta Thunb., browse oriental cherry fallen leaves, Japanese chestnut fallen leaves), and imported hay and straw: 5 species (timothy hay, tail fescue straw, annual ryegrass straw, klinegrass hay, alfalfa hay). Ten dairy goats (Saanen) were selected which had nearly the same body weight (25kg). The experiment was carried out on the dairy goats farm at Geumsan-Cun in Chungnam province. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. Among all the 25 species of roughages, the favorite intake species order by dairy goats was observed like this: mixed grasses, white clover, alfalfa and the lower intake species order was baled rice straw and rye silages. The dairy goats ate more roughages which had low fibrous contents, but high dry matter digestibility. On the other hand, compared to each roughage source, the goat's favorite roughage were grasses and legumes (34.6%) among the trial species. Based on the result, it is reconfirmed that the food habit of dairy goats seems to be closer to that of graters.

Anatomical Characteristics of Korean Mistletoe [Viscum album var. coloratum(Kom.) Ohwi] Stem (한국산 겨우살이 수간의 조직특성)

  • Lee, Bo-Duk;Park, Beyung-Su
    • Korean Journal of Plant Resources
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    • v.22 no.4
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    • pp.287-292
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    • 2009
  • Recently, the consumption of mistletoe[Viscum album var. coloratum(Kom.) Ohwi] is increasing because of its good medical effectiveness with the increased concern on the natural medicines and foods. The result obtained from the investigation on the stem tissues of the mistletoe and the oriental chestnut oak, a host plant species, are as follows. Haustorium from the seeds of the mistletoe after their sticking to the branches of the host plant penetrates into the bark where it forms the endophyte system through the active cell division. The endophyte grown in the cambium of the host plant makes the stems and leaves as the outer tissues in a certain time. Even through lignification of the host wood in the branches the oriental chestnut oak was not progressive, its tylosis coas developed partially assembly due to the formation of the endophyte. The stems of the mistletoe consisted of vascular tracheid, selereid, and ray and axial parenchyma, classified as a hardwood without vessels. The vascular tracheids seemed to take a role instead of the vessels in the mistletoe plant from the result that the pits of the vessels in the host branches are linked to the vessel-form tracheid in the mistletoe stems. The constituent ratio of the sclereid cells in the mistletoe stems increased with aging. Furthermore their ratio of the parenchyma cells was higher, which contained the more cell content, compared with the cells of the general woody plant species.

Study on the Hatching Characteristics and Diet of the Stick Insect, Baculum elongatum (Phasmida: Phasmatidae) for Artificial Mass Rearing

  • Lee, Jin Gu;Kim, Hee Dong;Kang, Chang Sung;Seo, Ae Gyeong;Lee, Sang Hyun;Park, Young Kyu;Lee, Young Bo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.26 no.1
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    • pp.61-66
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    • 2013
  • Baculum elongatum has several peculiarities such as parthenogenesis, unique external features, changing body color, and dropping oviposition, which makes it a potential economically useful insect. Oviposition was performed by females without fertilization by the sperm of males. The oviposition period was $42.2{\pm}22.7$ days and the number of eggs per female was $109.5{\pm}70.5$ eggs. The hatch rate was 73.3% at $25^{\circ}C$ and 66.7% after low temperature treatment ($8^{\circ}C$ for 60 days). In nature, B. elongatum overwinters as an egg, but it can be assumed from the results that cold temperatures were not required for hatching. The hatch rate was 98.2% in the treatment using floral foam, fermented sawdust, and leaves. The developmental period was $100.9{\pm}4.2$ days for eggs, $55.3{\pm}4.6$ days for nymphs, and $49.7{\pm}16.0$ days for adults. The length of the eggs was $0.33{\pm}0.0$ cm and the lengths of the nymphs were clearly distinguishable according to the instar stage. Clover(Trifolium repens) was an excellent diet as it was similar to the host plant and could be used as an alternative diet. The rate of reaching adulthood for the insects was 66.7% on an artificial diet containing 25% acacia leaves. For sustainable mass rearing of nymphs or adults of B. elongatum, a natural diet could be used such as acacia (Robinia pseudoacacia), white oak (Quercus aliena), chestnut (Castanea crenata var. dulcis), and bush clover (Lespedeza bicolor), or an alternative diet such as clover or artificial diet.

Screening of Natural Preservatives to Inhibit Kimchi Fermentation (김치의 선도유지를 위한 천연보존제의 탐색)

  • Moon, Kwang-Deog;Byun, Jung-A;Kim, Seok-Joong;Han, Dae-Suk
    • Korean Journal of Food Science and Technology
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    • v.27 no.2
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    • pp.257-263
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    • 1995
  • As a primary step to develop natural preservative for extending the shelf-life of kimchi, the effect of 102 edible plants, 21 antimicrobial agents and related compounds on kimchi fermentation was studied. Among 42 oriental medicinal plants tested, Baical skullcap and Assam indigo were found to be highly effective for maintaining the fresh state of kimchi. Although Bugbane, Red mangolia, Bushy sophora, Szechuan pepper, Chinese quince and Scisandre significantly inhibit the growth of Lactobacilli, their effect was not high enough to be used as raw materials for kimchi preservative. When the effect of 32 herbs and spices was tested, peppermint, cinnamon, lemon balm, clove, hop, rosemary, sage, horseradish and thyme showed high antimicrobial activity against kimchi microorganisms. Among them, the effect of clove ranked top. When it was added to fresh kimchi, initial cfu value ($2.4{\times}10^{6}cfu/g$) changed little even after 2 day's fermentation ($2.6{\times}10^{6}cfu/g$). Sensory test was not a good criteria to evaluate the effect of herbs and spices, since their highly specific flavors affected the taste of kimchies. Twenty eight fruits, vegetables and related plants were tested, but only leaves of pine tree, persimmon and oak leaves showed a significant bactericidal effect, finally contributing to the storage of kimchi. In addition, when 21 natural preservatives and other compounds were added individually to fresh kimchi, nisin and caffeic acid could inhibit fermentation.

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Secular chang of density, litterfall, phytomass and primary productivity in mongolian oak(quercus mongolica)forest (신갈나무 숲의 林木密度, 落葉量, 植物量 및 1次 純生産量의 經年 變化)

  • Kwak, Toung-Se;Kim, Joon-Ho
    • The Korean Journal of Ecology
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    • v.15 no.1
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    • pp.19-33
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    • 1992
  • The density, litterfall, phytomass, and primary productivity for 7 years in quercus mongolian forest locasted at mt. nambyengsan, pyeongchang-gun, gangwon provance in central part of korean peninsula were estimated quantitatively. at the first year in 1984, a stand had 1, 450 trees/ha in tree density, which was 0.67 of skewness and 0.54 of kurtosis in frequency distribution, however, at the 7th year in 1990, the stand had 1, 133 trees/ha in the density with 22%(or 316 tree/ha)in mortality, which was 1.16 of skewness and 1.89 of kurtosis in the frequency. annual mean litterfall was 5 ton DM/ha, which was composed of 68% of leaves, 17% of branches, 3% of bud scales, 9% of arcons and cups, 0.7% of flowers and others. the phytomass of tree layer for 7 years was gradually increased from 149.7 ton DM/ha at the first year to 188.5 ton DM/ha at the 7th year.annual net productivity for the tree layer studied ranged from 8.76 ton DM/ha.yr-1 to 11.62 ton DM/ha. yr-1 with heavy fluctuation year by year. average annual productivity of the stand of trunk, branches, leaves and roots for 7 years were 4.42, 0.67, 3.85 and 1.29 ton DM/ha.yr-1, respectively. turnover rate of the stand was 6.9% at the first year and 5.6% at the 7th year. such fluctuation of the productivity was caused by the chang of density, mortality, mortality and turnover rate.

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Ecological Study of Pseudoips fagana (Lepidoptera: Noctuidae) (쌍줄푸른밤나방 (Pseudoips fagana )의 생태학적 특성)

  • Kwon, Young-Dae;Park, Il-Kwon
    • Korean journal of applied entomology
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    • v.50 no.2
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    • pp.167-170
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    • 2011
  • Pseudoips fagana Fabricius belongs to the subfamily Chloephorinae (Lepidoptera: Noctuidae). Eggs of Pseudoips fagana were white and oviposited on oak leaves. There were five P. fagana larval instars, which were light green in color with four white crosslines. The larval period was approx. 24.4 days ($25^{\circ}C$, 16L:8D). Pupae were covered with a light-green membrane and silk. The wingspan of adults was approx. 15 to 18 mm. The color of the forewings of both sexes was bright green. The color of the hindwings was yellow in males and and white in females. First emergence was from early May to late June, and the second emergence was from early July to late August. Pseudoips fagana over-wintered as pupae.

The Effects of Dance Sports Program on Health Promotion in Rural Women (댄스스포츠 프로그램이 농촌여성의 건강증진에 미치는 효과)

  • Kim, Dong-Oak;Lee, Hyeon-Soon;Kwon, Young-Sook
    • Journal of Korean Biological Nursing Science
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    • v.14 no.2
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    • pp.84-93
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    • 2012
  • Purpose: This study was performed to identify the effects of dance sports programs on the parameters of health promotion (blood pressure, pulse, glucose, blood lipids, musculoskeletal pain, fatigue, self-efficacy, and depression) in rural women. Methods: A non-equivalent control group pretest-posttest experimental design was used. The study subjects were 38 women who were aged 45-78 and cultivating perilla leaves in a rural area. An experimental group (n=20) received the dance sports program from December 3rd, 2009 to June 3rd, 2010 and a control group (n=18) did not. Data was collected 3 times (before the program, 12 during and 24 weeks after the program) and were analyzed with $X^2$ test, t-test, and Repeated Measures ANOVA using SPSS/WIN 18.0. Results: For the experimental group, significant improvement was found in high density lipoprotein cholesterol (HDL-C), low density cholesterol (LDL-C), triglyceride (TG) musculoskeletal pain, fatigue, self-efficacy, and depression as compared to the control group. Conclusion: The findings of the study indicates that the dance sports program is effective on health promotion for rural women. Therefore, dance sports programs can be recommended for health promotion of rural residents.

Enterotoxemia caused by Clostridium perfringens type A in Formosan deer (꽃사슴의 Clostridium perfringens A형에 의한 장독혈증 발생 보고)

  • 이청산;한성태;곽학구;박경재;현공율;조우영;이종인;배유찬;진영화
    • Korean Journal of Veterinary Service
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    • v.25 no.2
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    • pp.127-133
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    • 2002
  • The case reports for clostridium type A enterotoxemia in Formosan deer have rarely been reported. This paper describes a natural case of type A enterotoxemia in farmed Formosan deer in Cheongwon-gun. A dead, male 10-month-old Formosan deer was submitted to Chungbuk Livestock and Veterinary Research Institute, March 24, 2001 and examined. That deer was fed with assorted grain feed, oak leaves, acorn and bean curd. Grossly there was no visible external change. Despite of the carcass being examined within 12 hours of death, there was a quite degree of posonortem decomposition. There was severe hemorrhage in the serosa of abomasum and small intestine. Much blood tinged and watery contents were contained in those organs. Also there were severe swelling of spleen, some red foci in hepatic parenchyma. Microscopically there were severe congestion and hemorrhage in mucosa submucosa, muscular layer, and serosa of abomasum and small intestine. Also spleen and pancreas showed severe Congestion and hemorrhage. There were multifocal hemorrhage with hepatic necrosis in periportal area and focal mononuclear cell deposition in sinusoid. In bacterial culture for small intestine, Cl perfringens was isolated. By toxin typing for the strain, that had $\alpha$ -toxin belonged to type A. In electronmicroscopy for feces, no vims particle was detected. Considering clinical signs, gross lesions, microscopic lesions, bacterial culture, and toxin typing of the isolate, this case was diagnosed as enterotoxemia by Cl perfringens type A.