• 동의생리병리학회지
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• 제31권5호
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• pp.277-283
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• 2017

Activated T helper 2 (Th2) immune function is hallmark of various allergic diseases. We investigated the anti-allergic effect of Jagamcho-tang extract(JE) on ovalbumin(OVA)-induced atopic dermatitis mice model and OVA-stimulated splenocytes isolated from the mice. Mice were intraperitoneally injected OVA/alum solution 2 times at interval of 14 days, followed by oral administration of JE for 7 days. After administration, mice were subcutaneously injected with OVA in ear. JE treatment reduced ear swelling and infiltration of inflammatory cells in ear. Serum levels of interleukin(IL)-4 and immunoglobulins, such as total-IgE and OVA-specific IgE, were decreased in JE treated group. Furthermore, JE treatment decreased OVA-induced Th2-associated cytokines like IL-4, IL-5 and IL-13 mRNA levels in splenocytes. In conclusion, JE reduced allergic immune response via IgE production and Th2 response in OVA-sensitized mice, suggesting that JE could be useful prescription for allergic diseases including atopic dermatitis.

• Biomolecules & Therapeutics
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• 제16권3호
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• pp.237-242
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• 2008

The effect of Picrorrhiza Rhizoma (PR) aqueous extracts were evaluated on 2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic model. Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. Three different concentrations of PR extracts (300,150 and 75mg/kg) were orally administered to DNP-OVA sensitization mice once a day for 7 days with reference materials; dexamethasone (15mg/kg, intraperitoneal treatment). End of 7 days oral administration of PR extracts or intraperitoneal treatment of dexamethasone, the changes on the edematous changes and scratching behavior were measured. Immediate after DNFB challenge on ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected with anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these DNFB-induced increases on ear and paw thicknesses, weights and scratching behaviors were decreased by treatment of all three different dosages of PR extracts and dexamethasone, respectively. In addition, the increases of anterior skin thicknesses were also dramatically inhibited by treatment of all three different dosages of PR extracts and dexamethasone at histopathological observations. The results obtained in this study suggest that oral treatment of PR extracts also has relatively favorable effects on allergic dermatitis.

• Toxicological Research
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• 제26권2호
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• pp.123-130
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• 2010

The effect of DHU001, a mixed herbal formula consisted of 7 types aqueous extracts for various respiratory disorders were evaluated on 2,4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic model. Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. Two different dosages of DHU001 (300 and 150 mg/kg) were orally administered to DNP-OVA sensitization mice once a day for 7 days with reference material, dexamethasone (15 mg/kg, intraperitoneal treatment). End of 7 days oral administration of DHU001 extracts or intraperitoneal treatment of dexamethasone, the changes on the edematous changes and scratching behavior were measured. Immediate after DNFB challenge on ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected with anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these contact dermatitis signs induced by DNFB treatment were reduced by treatment of the both different dosages of DHU001 and dexamethasone, respectively. The results obtained in this study suggest that oral treatment of DHU001 extracts also has relatively favorable effects on contact dermatitis.

• 대한한방내과학회지
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• 제30권4호
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• pp.788-798
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• 2009

목적 : 이 연구는 백련초의 천식마우스 모델에 대한 효과를 조사하기 위해 수행하였다. 방법: 폐 세척액의 전체 세포와 호산구 수 그리고 폐조직으로의 면역세포의 침윤은 헤마톡실린과 에오신 염색으로 관찰 하였다. Interleukin (IL)-4의 발현량은 Enzyme-linked immunosorbent assay을 이용하여 측정하였으며, NO는 Griess roagont을 이용하였다. 결과 : 천식 병리현상의 하나인 Ovalbumin(OVA)에 의한 전체 세포와 호산구 수의 증가는 백련초 추출물 (200 mg/kg)을 처리한 군에서 감소하였다 (respectively, p<0.001 and p<0.01). 또한 OVA에 의한 증가된 면역세포의 침투는 백련초 추출물에 의해 감소하였다 (p < 0.01). 호산구의 침윤증상은 interleukin (IL)-4 그리고 5를 포함한 T helper (Th) 2 반응과 관련있기 때문에 폐세척액에서 IL-4의 발현량을 조사하였다. OVA에 증가한 IL-4는 백련초 추출물에 의해 감소하였다 (p < 0.05). 천식이 염증성 질병이기 때문에, lipopolysaccharide (LPS)로 자극한 RAW 264.7 세포에서 nitrite (NO) 생성을 살펴보았다. LPS에 의해 증가한 NO 생성을 백련초 추출물 (50, 100 and 200 mg/ml)에서 감소하였다 (respectively, p < 0.05, p<0.01 and p<0.05). 결론 : 이런 결과들은 백련초가 천식의 호산구 침윤에 저해효과를 가지고 있다고 나타내며, 백련초 추출물의 천식을 포함한 염증성 질환의 치료에 치료후보물질로 제안할 수 있다.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2003년도 추계학술대회초록집
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• pp.7-7
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• 2003

Histological examination of biopsy or postmortem lung tissue from patients with asthma usually reveals thickened airway walls. This change is called airway remodeling, which is characterized by airway eosinophilia, hyperplasia of goblet cells and smooth muscle, and subepithelial fibrosis [1,2]. In this study, we investigated the time-course functional, morphological, biochemical changes of remodeling in a ovalbumin (OVA)-induced murine chronic asthma model. (omitted)

• 대한본초학회지
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• 제25권2호
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• pp.55-63
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• 2010

Objectives : In this study, we investigated anti-allergic effect of Okbyeongpungsan-Hap-Changijasan (KOB01) in allergic rhinitis(AR) experimental animals and mast cells. Methods : The potential anti-allergic effect of KOB01 was investigated in a rat model of compound 48/80-induced systemic anaphylactic shock and a mouse of ovalbumin(OVA)-induced AR, and human mast cell line, HMC-1 culture. Each animals were divided into four groups: normal, control, KOB01-treated(100 and 200 mg/kg) and anti-histamine drug, dosodium cromoglycate (DSCG)-treated(50 mg/kg). Animals were orally treated with KOB01 and DSCG and intraperitoneally injected with compound 48/80($10\;{\mu}g/kg$) or sensitized with 0.1% OVA. The mortality and serum histamine levels were measured in compound 48/80-induced anaphylatic rats. The histological changes in nasal mucosa were investigated in OVA-induced AR mice. Also, mast cell degranulation was observed in compound 48/80-stimulated HMC-1 cells. Results : KOB01 increased mortality and significantly decreased serum histamine levels in compound 48/80-induced anaphylatic rats. The abnormal histological changes such as expansion of grandular cells and hypertrophy of epithelium in nasal mucosa of OVA-induced AR mice was improved by KOB01 treatment nearby a normal group. Therefore, KOB01 inhibited compound 48/80-induced degranulation in HMC-1 cells. Conclusions : These results indicate that KOB01 decrease allergic response through suppressing the mast cell activation in AR and suggest a potential role for KOB01 as a source of anti-allergic agents for use in allergic disorders including of AR.

• Journal of Ginseng Research
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• 제46권4호
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• pp.550-560
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• 2022

Background: The effect of ginsenoside Rh2 (G-Rh2) on mast cell-mediated anaphylaxis remains unclear. Herein, we investigated the effects of G-Rh2 on OVA-induced asthmatic mice and on mast cell-mediated anaphylaxis. Methods: Asthma model was established for evaluating airway changes and ear allergy. RPMCs and RBL-2H3 were used for in vitro experiments. Calcium uptake, histamine release and degranulation were detected. ELISA and Western blot measured cytokine and protein levels, respectively. Results: G-Rh2 inhibited OVA-induced airway remodeling, the production of TNF-α, IL-4, IL-8, IL-1β and the degranulation of mast cells of asthmatic mice. G-Rh2 inhibited the activation of Syk and Lyn in lung tissue of OVA-induced asthmatic mice. G-Rh2 inhibited serum IgE production in OVA induced asthmatic mice. Furthermore, G-Rh2 reduced the ear allergy in IgE-sensitized mice. G-Rh2 decreased the ear thickness. In vitro experiments G-Rh2 significantly reduced calcium uptake and inhibited histamine release and degranulation in RPMCs. In addition, G-Rh2 reduced the production of IL-1β, TNF-α, IL-8, and IL-4 in IgE-sensitized RBL-2H3 cells. Interestingly, G-Rh2 was involved in the FcεRI pathway activation of mast cells and the transduction of the Lyn/Syk signaling pathway. G-Rh2 inhibited PI3K activity in a dose-dependent manner. By blocking the antigen-induced phosphorylation of Lyn, Syk, LAT, PLCγ2, PI3K ERK1/2 and Raf-1 expression, G-Rh2 inhibited the NF-κB, AKT-Nrf2, and p38MAPK-Nrf2 pathways. However, G-Rh2 up-regulated Keap-1 expression. Meanwhile, G-Rh2 reduced the levels of p-AKT, p38MAPK and Nrf2 in RBL-2H3 sensitized IgE cells and inhibited NF-κB signaling pathway activation by activating the AKT-Nrf2 and p38MAPK-Nrf2 pathways. Conclusion: G-Rh2 inhibits mast cell-induced allergic inflammation, which might be mediated by the AKT-Nrf2/NF-kB and p38MAPK-Nrf2/NF-κB signaling pathways.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1071-1077
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• 2017

A rise in the occurrence of allergic diseases is attributed to the dysregulated balance of type 1/type 2 immunity, where type 2 T-helper (Th2) cells predominate over type 1 T-helper (Th1) cells, leading to an abnormally increased production of IgE in response to unharmful antigens. Kimchi, a traditional Korean fermented food, is a rich source of beneficial lactic acid bacteria. In this study, we investigated the ability of Enterococcus faecium FC-K derived from kimchi to induce type I immunity in the presence of Th2 polarizing conditions in vitro and in vivo. Stimulation of mouse peritoneal macrophages with E. faecium FC-K induced the production of tumor necrosis factor alpha, interleukin (IL)-6, and IL-12. Under the in vitro Th2 conditions in which splenic T cells were activated in the presence of IL-4, E. faecium FC-K enhanced the ability of T cells to produce interferon $(IFN)-{\gamma}$. Using the ovalbumin (OVA)-induced allergy model, male BALB/c mice receiving E. faecium FC-K reduced the serum level of total IgE, but not that of OVA-specific IgE. Furthermore, the population of activated splenic B cells during OVA immunization was decreased in E. faecium FC-K-treated mice, accounting for a reduction of total IgE in the serum. Restimulating splenocytes from OVA-immunized mice with OVA ex vivo resulted in an increased production of $IFN-{\gamma}$, with no effect on IL-4, in E. faecium FC-K-treated mice. These observations provide the evidence that E. faecium FC-K can be a beneficial probiotic strain that can modulate the Th2-mediated pathologic response.

• Clinical and Experimental Pediatrics
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• 제53권4호
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• pp.481-487
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• 2010

목 적: 최근 여러 연구결과 천식을 포함한 알레르기질환의 발병에 소아 성장시기에 알레르겐이나 내독소(LPS)등과 같은 물질에 노출이 중요한 역할을 하는 것으로 제시되었다. 이에 연구자들은 출생 초기에 기도 점막을 통한 알레르겐과 내독소에의 노출이 성장한 후에 알레르기 기도염증과 과반응성의 발생에 미치는 효과를 생쥐 모델을 통하여 규명하고자 본 연구를 시행하였다. 방 법: 실험동물은 무균 상태의 생후 1주이내의 암컷 BALB/c 생쥐를 사용하였다. 실험동물들에게는 각각 신생생쥐시기에 생리식염수, 1% ovalbumin (OVA), $1.0{\mu}g$ LPS, $1.0{\mu}g$ LPS in 1% OVA를 각 비공을 통하여 투여하였다. 실험동물은 연구 시작 제 35일부터 10일간 5% OVA로 감작시켰으며 최종 기도 감작 10일 후부터 3일간 1% OVA로 기도 항원유발을 시행하였다. 최종 기도유발 48시간 후 체적검사(plethysmography)를 이용하여 비특이적 기도과반응성 검사(Methcholine challenge test)를 시행하였으며 검사 후 실험동물을 희생시켜 검체를 취하여 BAL액내 세포분획, 사이토카인, 혈청 면역글로불린을 측정하였다. 결 과: 1) 메타콜린에 의한 기도과반응성은 생후 초기에 OVA, LPS, OVA/LPS를 투여한 군에서 생리식염수를 투여한 군에 비해 통계적으로 유의하게 억제되었다. 2) OVA, LPS, OVA/LPS를 투여한 군에서 BAL 액내의 호산구수와 IL-4, IL-5가 유의하게 낮았다. 3) 혈청내 OVA 특이 IgE는 OVA, LPS, OVA/LPS 투여군에서 유의하게 감소되었다. 결 론: 본 연구 결과 신생생쥐 시기의 점막을 통한 항원, 내독소의 노출이 성숙한 후 항원에 의한 기도염증 및 과반응성의 발생을 억제하였으며 향후 이러한 효과의 작용기전에 대한 연구가 필요할 것으로 생각한다.

• 동의생리병리학회지
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• 제20권3호
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• pp.548-556
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• 2006

This study was to evaluate the effect of Samjayangchin-tang (STYCT) on mouse Th1 and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of STYCT extract were measured as well as the amount of ${\beta}-hexosaminidase$ in RBL-2H3 cells and the levels of $TNF-{\alpha}$ and IL-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with STYCT extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total IgE, OVA-specific IgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4 T cell were stimulated with anti-CO3 and anti-CD28 for 48 hours in various concentrations of STYCT extract, it decreased proliferation of CD4 cells. CD4 T cells under Th1/Th2 polarizing conditions for 3 days with STYCT resulted in mild decrease of IFN- g in Th1 cells and significant decrease of IL-4 in Th2 cells. STYCT extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}-hexosaminidase$ in RBL-2H3 cells. Treatment of STYCT extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in IL-6 production. Oral administration of STYCT extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum IgE and OVA-specific IgE by 53% and 44%, respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 54% and significant decrease of IL-4 and IL-5 by 42%, and 29%, respectively. The results show that STYCT does not strongly induce mouse T cells to transform into Th1 or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.