Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Samjayangchin-tang on Cytokine Levels of Mouse Th1/Th2 Cells and Anti-allergic Activity in Ovalbumin-sensitized Allergic Inflammation Model

삼자양친탕(三子養親湯) 물 추출물(抽出物)이 마우스 Th1/Th2 분화 및 알레르기 염증반응 조절에 미치는 효과

  • Park, Joung-Su (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Myung, Eu-Gene (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Park, Sung-Min (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
  • 박종수 (경희대학교 한의과대학 병리학교실) ;
  • 강희 (경희대학교 한의과대학 병리학교실) ;
  • 명유진 (경희대학교 한의과대학 병리학교실) ;
  • 박성민 (경희대학교 한의과대학 병리학교실) ;
  • 심범상 (경희대학교 한의과대학 병리학교실) ;
  • 김성훈 (경희대학교 한의과대학 병리학교실) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실) ;
  • 안규석 (경희대학교 한의과대학 병리학교실)
  • Published : 2006.06.25
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Abstract

This study was to evaluate the effect of Samjayangchin-tang (STYCT) on mouse Th1 and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of STYCT extract were measured as well as the amount of ${\beta}-hexosaminidase$ in RBL-2H3 cells and the levels of $TNF-{\alpha}$ and IL-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with STYCT extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total IgE, OVA-specific IgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4 T cell were stimulated with anti-CO3 and anti-CD28 for 48 hours in various concentrations of STYCT extract, it decreased proliferation of CD4 cells. CD4 T cells under Th1/Th2 polarizing conditions for 3 days with STYCT resulted in mild decrease of IFN- g in Th1 cells and significant decrease of IL-4 in Th2 cells. STYCT extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}-hexosaminidase$ in RBL-2H3 cells. Treatment of STYCT extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in IL-6 production. Oral administration of STYCT extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum IgE and OVA-specific IgE by 53% and 44%, respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 54% and significant decrease of IL-4 and IL-5 by 42%, and 29%, respectively. The results show that STYCT does not strongly induce mouse T cells to transform into Th1 or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.

Keywords

References

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