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Effect of Samjayangchin-tang on Cytokine Levels of Mouse Th1/Th2 Cells and Anti-allergic Activity in Ovalbumin-sensitized Allergic Inflammation Model  

Park, Joung-Su (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Kang, Hee (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Myung, Eu-Gene (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Park, Sung-Min (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Kim, Sung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.3, 2006 , pp. 548-556 More about this Journal
Abstract
This study was to evaluate the effect of Samjayangchin-tang (STYCT) on mouse Th1 and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of STYCT extract were measured as well as the amount of ${\beta}-hexosaminidase$ in RBL-2H3 cells and the levels of $TNF-{\alpha}$ and IL-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with STYCT extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total IgE, OVA-specific IgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4 T cell were stimulated with anti-CO3 and anti-CD28 for 48 hours in various concentrations of STYCT extract, it decreased proliferation of CD4 cells. CD4 T cells under Th1/Th2 polarizing conditions for 3 days with STYCT resulted in mild decrease of IFN- g in Th1 cells and significant decrease of IL-4 in Th2 cells. STYCT extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}-hexosaminidase$ in RBL-2H3 cells. Treatment of STYCT extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in IL-6 production. Oral administration of STYCT extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum IgE and OVA-specific IgE by 53% and 44%, respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 54% and significant decrease of IL-4 and IL-5 by 42%, and 29%, respectively. The results show that STYCT does not strongly induce mouse T cells to transform into Th1 or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.
Keywords
STYCT; Th1/Th2; IL-4; IFN-g; allergy;
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