• Biomolecules & Therapeutics
• /
• v.10 no.2
• /
• pp.129-135
• /
• 2002

HM10411 is a recombinant granulocyte-colony stimulating factor (rG-CSF) that has been developing as a drug for neutropenia. In this study, antigenic potential of HM10411 was examined by active systemic anaphylaxis (ASA) in guinea pigs and passive cutaneous anaphylaxis (PCA) in guinea pig-guinea pig system. HM10411 was subcutaneously administered at 0,5, and 50 mg/kg and also as a suspension with adjuvant (50 mg/kg+FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control responses. In the ASA test, no symptoms except urination and evacuation that were considered as physiological phenomena were observed at 0 and 5 mg/kg. Two of 5 animals at 50 mg/kg showed sneering, dyspnea, or cyanosis. All animals in the adjuvant mixture group showed severe symptoms of anaphylatic shock and 3 of them died. In the PCA test, no antibody against HM 10411 was detected in the sera from the animals sensitized with 0 or 5 mg/kg. Only 1 serum sample from the animals immunized with 50 mg/kg showed positive reaction against HM10411, while all 5 sera collected from the HM10411 and FCA mixture group contained the HM10411-specific antibodies. These results suggest HM10411 is considered to have antigenicity In guinea pig.

• Journal of Pharmacopuncture
• /
• v.14 no.4
• /
• pp.23-32
• /
• 2011

Objectives: This study was performed to examine the antigenic potential of pure melittin (Sweet Bee Venom - SBV) extracted from the bee venom by utilizing protein isolation method of gel filtration. Methods: All experiments were conducted at Biotoxtech (Chungwon, Korea), authorized a non-clinical studies institution, under the regulations of Good Laboratory Practice (GLP). Antigenic potential of SBV was examined by active systemic anaphylaxis (ASA) and passive cutaneous anaphylaxis (PCA) in guinea pigs. SBV was subcutaneously administered at 0.07 and 0.28mg/kg and also as a suspension with adjuvant (Freund's complete adjuvant: FCA). Ovalbumin (OVA) as a suspension with adjuvant was used to induce positive control response ($5mg/m{\ell}$-FCA). Results: 1. In the ASA test, experimental groups showed some symptoms of anaphylaxis like piloerection, hyperpnea and staggering gait. 2. In the PCA test, low dosage group did not show any antibody responses, whereas high dosage group showed positive responses. 3. In the weight measurement and clinical observation, experimental groups didn't show any significant changes compared with control group. 4. In the autopsy of body, the abnormalities of lung were detected in the corpse. This means that the cause of death may induced anaphylactic shock. Conclusions: Above findings suggested that SBV had antigenic potential in guinea pig. Further studies on the subject should be conducted to yield more concrete evidences.

• The Korean Journal of Physiology and Pharmacology
• /
• v.22 no.1
• /
• pp.81-89
• /
• 2018

This study evaluated the anti-asthmatic activities of 2,6-di-tert-butyl-4-hydroxymethylphenol (DBHP) that is a potent phenolic antioxidant in edible vegetable oil. The effects of DBHP on bronchial asthma were evaluated by determining the specific airway resistance (sRaw) and tidal volume (TV) during the immediate asthmatic response (IAR) and the late-phase asthmatic response (LAR) in guinea pigs with aerosolized ovalbumin-induced asthma. Recruitment of leukocytes and the levels of biochemical inflammatory mediators were determined in the bronchoalveolar lavage fluids (BALFs), and histopathological surveys performed in lung tissues. DBHP significantly inhibited the increased sRaw and improved the decreased TV on IAR and LAR, and also inhibited recruitment of eosinophils and neutrophils into the lung, and release of biochemical inflammatory mediators such as histamine and phospholipase $A_2$ from these infiltrated leukocytes, and improved pathological changes. However, anti-asthmatic activities of DBHP at oral doses of 12.5 to 50 mg/kg was less than those of dexamethasone (5 mg/kg, p.o.) and cromoglycate (10 mg/kg, p.o.), but more potent or similar to that of salbutamol (5 mg/kg, p.o.). These results in the present study suggest that anti-asthmatic effects of DBHP in the guinea pigs model of OVA-induced asthmatic responses principally are mediated by inhibiting the recruitments of the leukocytes and the release of biochemical inflammatory mediators from these infiltrated leukocytes.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.1
• /
• pp.15-20
• /
• 2013

Urinary bladder squamous cell carcinoma (SCC), one of the most common neoplasms in Egypt, is attributed to chronic urinary infection with Schistosoma haematobium (Schistosomiasis). The proto-oncogene c-KIT, encoding a tyrosine kinase receptor and implicated in the development of a number of human malignancies, has not been studied so far in schistosomal urinary bladder SCCs. We therefore determined immunohistochemical (IHC) expression of c-KIT in paraffin sections from 120 radical cystectomies of SCCs originally obtained from the Pathology Department of Suez Canal University (Ismailia, Egypt). Each slide was evaluated for staining intensity where the staining extent of >10% of cells was considered positive. c-KIT overexpression was detected in 78.3% (94/120) of the patients, the staining extents in the tumor cells were 11-50% and >50% in 40 (42.6%) and 54 (57.4%) respectively. The positive cases had 14.9%, 63.8%, 21.3% as weak, moderate and strong intensity respectively. Patients with positive bilharzial ova had significantly higher c-KIT expression than patients without (95.2% vs. 38.9%, P=0.000). Mutation analysis of exons 9-13 was negative in thirty KIT positive cases. The high rate of positivity in SBSCC was one of the striking findings; However, CD117 may be a potential target for site specific immunotherapy to improve the outcome of this tumor.

• Biomedical Science Letters
• /
• v.12 no.3
• /
• pp.177-183
• /
• 2006

To demonstrate the effect of formulation conditions and evaluations of structural integrity from ovalbumin containing poly lactide glycolide copolymer (PLGA) microspheres for Vaccine delivery, OVA microspheres were prepared by a W/O/W multiple emulsion solvent extraction technique. Dichloromethan (DCM) and Ethyl acetate (EA) were applied as an organic phase and poly vinyl alcohol (PVA) as a secondary emulsion stabilizer. Microspheres were characterized for particle size, morphology (optical microscopy and Scanning Electron Microscope (SEM)). Protein denaturation was evaluated by size exclusion chromatography (SEC), SDS-PAGE and isoelectric focusing (IEF). Residual organic solvent was estimated by gas chromatography (GC) and differential scanning calorimetry (DSC). Optical photomicrograph and SEM revealed that micro spheres were typically spherical but various morphologies were observed. Mean particle size $(d_{vs})$ of microspheres were in the range of $3{\sim}50{\mu}m$. Also, The protein stability was not affected by the fonnulation process and residual organic solvent was beyond the detection below 0.1ppm. These results demonstrated that micro spheres might be a good candidate for the parenteral vaccine delivery system.

• Journal of the Korea Safety Management & Science
• /
• v.16 no.3
• /
• pp.327-333
• /
• 2014

Changes in the business environment in which intense and sustained growth and survival must meet a variety of customer needs (Q, C, D) and business side of the enterprise for profit structure reformation is absolutely necessary for innovation activities. So far, management of innovation in method BPR, PI, OVA, 6 Sigma, Strategic Purchasing, PPM, SCM etc. are being introduced. However, they have a limit of partial optimization and improvement-oriented techniques. So this paper studied the TPI(Total Profit Innovation) application in order to derive empirical methodology to maximize profitability for the domestic S foundry factory. To this end, long-term gains through structural analysis and intensity analysis to ensure continued growth and profitability strategy are devised through management Innovation analysis. And improvement projects was presented to solve main issues of five categories(Inventory, Sales Mix, Cost, Quality Cost, Skill and Work-load) We will expect the office productivity improvement and financial performance improvement and then continually accumulate and review the results.

• Parasites, Hosts and Diseases
• /
• v.24 no.2
• /
• pp.209-212
• /
• 1986

The present study was undertaken to evaluate the present status of intestinal parasitic infection in Seoul area, Korea. During the period from June 1985 to July 1986, a total of 5,251 stool samples were collected in Department of Clinical Pathology, Hanyang University Hospital and examined by formalin-ether sedimentation technique once for helminth ova and protozoan cysts. The results were summarized as follows: 1. The overall egg positive rate of intestinal helminthes was 2.53%; and 1.43% for Clonorchis sinensis, 0.7% for Trichuris trichiura, 0.13% for Metagonimus yokogawai, 0.06% for hookworm, and 0.02% for Ascaris lumbricoides, Diphyllobothrium latum and Hymenolepis nana, respectively. 2. The overall cyst positive rate of intestinal protozoa was 1.07%. Cyst positive rate was 0.06% for Entamoeba histolytica and 0.13% for Giardia lamblia, respectively. 3. Higher prevalence of intestinal parasitic infection was observed in male and in $21{\sim}50$ year-old groups. 4. The highest prevalence of Clonorchis sinensis infection was observed in 40th age group (3.4%), and male (2.3%) was more infected than female (0.7%) in general.

• Journal of the Korean Society for Precision Engineering
• /
• v.19 no.7
• /
• pp.43-50
• /
• 2002

This paper presents the new gait implementation of a biped robot with smooth walking using 3-dimensional continuous trunk motion and kick action of ankle joints. Trajectory generation ova trunk is performed not on a unit gait but on a whole walking interval. In applying kick action such as heel-touch or toe-off, varying coordinate system was employed for the simplification of the kinematic analysis. Desired ZMP (zero moment point) is also changed to implement the efficient kick action. As a result, balancing motion of the proposed gait was much more decreased than that of conventional one. Moreover, robot\\`s walking behavior is very smooth, natural and similar to the pace of a human. The walking experiment system is composed of eight AC servo motors and a DSP controller. The walking simulation and the experimental results are shown using the proposed new walking algorithm.

• Korean Journal of Veterinary Service
• /
• v.18 no.1
• /
• pp.33-40
• /
• 1995

As a series of basic study for the prevention of zoonotic parasites, fecal samples and internal organs of cats in Iri and its vicinity were examined for helminth and their ova or protozoan oocysts from November 1994 to March 1995. The results obtained were summarized as follows； Out of the 133 samples, 101(75.9%) were positive and 135(101.5%) were cumulative positive for parasites. The following were identified in the samples and positive numbers of each species detected were ; Toxocura cari, 51；Isospora spp., 27；Spirometra erinacei, 26；Tasnka taentaeformis, 24；Diphyllobothrium latum, 4；and Capillaria spp., 3, respectively. In analyzing the state of infection, 64.9%(61 heads) of all the cats inspected had a single infection, while the rest(excluding the 32 uninfected cats) had mixed infections : 27.6%(26 heads), 6.4%(6 heads) and 1.1%(1 heads) had double, triple, quadruple Infections, respectively. From these results, it can be concluded that since the helminth, zoonotic parasites, in Korean autochthonal cats cause not only various disorders, but also can be very harmful to human beings, they are considered to be of great significance to public health.

• Journal of the Korean Society of School Health
• /
• v.19 no.2
• /
• pp.13-24
• /
• 2006

Purpose : The purpose of this study was to develop an obese elementary students education program available for blended learning environments, which refered to a combination of ofline and online learning. And it's also intended to verify its efect to bost the eficiency of obese schol children's learning about obesity.Methods : The subjects were 52(experimental group=21, control group=31 ) fourth, fifth and sixth graders who OVA. Results : The obese elementary students education program tailored to blended learning setting wasn't more conducive to lowering the obesity level of the obese students t han the typical obese elementary students education program. But the obese elementary students education was more efective than the typical one in improving the knowle dge of the obese students about obesity, and in enhancing the academic self-eficacy of the children. Conclusion : As the obese elementary students education program tailored to blended learning setting was effective in boosting the relevant knowledge and academic self-efficacy of the children, sustained research efforts should be put into developing obese elementary students education programs available for both online and offline to help students to access information on obesity and manage it better.