• Title/Summary/Keyword: OFP

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Identification of novel genes for improvement of downy mildew resistance in Zea mays (옥수수의 노균병 저항성 증대를 위한 저항성 유용유전자 발굴)

  • Min, Kyeong Do;Kim, Hyo Chul;Kim, Kyung-Hee;Moon, Jun-Cheol;Lee, Byung-Moo;Kim, Jae Yoon
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.493-502
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    • 2019
  • Maize (Zea mays L.) is a C4-plant and one of the three major crops grown worldwide. Because of its high productivity, maize is considered as one of the most important food and feed stocks in the world. Recently, bioethanol from maize was predominantly generated in the USA and Brazil. Infection of maize by several diseases resulted in a huge disaster and prevented maize production. Downy mildew, caused by Peronosclerospora sorghi, is one of the most serious diseases of maize. Despite efforts to develop downy mildew-resistant cultivars or seed treatment with metalaxyl, downy mildew persists as a serious pathogen and is still prevalent in specific geographical locations. Analysis of soils infected with downy mildew and investigation of candidates associated with downy mildew resistance is an attractive method to overcome downy mildew damage in maize. In a previous study, we reported that maize chromosome 6 carries a possible candidate gene for downy mildew resistance. Using bioinformatics tools and RT-PCR analysis, five novel genes including bZIP, OFP transcription factor, and Ppr were identified as candidate genes associated with downy mildew resistance.

Requirement management program for the Development of the Avionics Operational Flight Program (항공전자장비 비행운용프로그램 개발을 위한 요구도 관리 시스템)

  • Park, Kyoung-Choon;Seo, Jung-Bae;Bae, Jong-Min;Kang, Hyun-Syug
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.15 no.9
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    • pp.2050-2058
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    • 2011
  • The objective of this thesis is to research the software requirement management system for the development of operational flight program (OFP) which is embedded in aircraft avionics. It offers the function to record the requirement revision log. In fact, the COTS (commercial off-the-shelf) program which has similar function already exists in the market. But this kind of software programs are too complicated and relatively expensive in acquisition. Furthermore, they require too expensive maintenance cost, medium sized business company, this thesis intended to provide the software requirement management system that can be simply installed and easily used.

Purification and Characterization of a Serine Proteinase from Acanthamoeba culbertsoni

  • Park, Ki-Won;Song, Chul-Yong
    • BMB Reports
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    • v.29 no.5
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    • pp.455-461
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    • 1996
  • A serine proteinase was purified from Acanthamoeba culbertsoni by 41~80% ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography and gel filtration chromatography. The molecular weight of the purified enzyme was estimated to be 108.0 kDa by gel filtration chromatography and 54.0 kDa by SDS-PAGE. Therefore, the purified enzyme seemed to be a dimer. Isoelectric point was 4.5. The enzyme activity was highly inhibited by the serine proteinase inhibitors diisopropyl fluorophosphate (OFP) and phenylmethyl sulfonylfluoride (PMSF). It had a narrow pH optimum of 6.5~7.5 with a maximum at pH 7.0. These data suggested that the purified enzyme was a neutral serine proteinase. Optimal temperature was $37^{\circ}C$. It was stable for at least 16 h at $4^{\circ}C$ and $37^{\circ}C$, but it was rapidly inactivated at $65^{\circ}C$ The activity of the purified enzyme was not influenced significantly by $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$ or $Ca^{2+}$. However, the enzyme activity was highly inhibited by $Hg^{2+}$ The enzyme degraded type I collagen and fibronectin, but not BSA, hemoglobin, lysozyme, immunoglobulin A or immunoglobulin G.

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Development of Verification Environment for Flight Safety Critical Software using NEXUS (NEXUS를 이용한 비행안전 필수 소프트웨어 검증환경 개발)

  • Yoon, Hyung-Sik;Han, Jong-Pyo
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.40 no.6
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    • pp.548-554
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    • 2012
  • Verification and validation of operational software of the flight control computer, which is flight safety critical, is very important to prove correctness and faultness of the software. To verify the real-time softare requirement on operational software of flight control computer, real-time software internal parameter and variable monitoring technics on hardware-in-the-loop environment, similar to on-flight environment, is required. This paper describes flight safety critical software validation and verificiation environment using standard debugging interface, NEXUS 5001.

Selenium arrest G1/S phase of cell cycle in LNCaP human prostate cancer cells (사람 전립선암세포주인 LNCaP에서 셀레늄의 G1/S 세포주기억제에 관한 연구)

  • Nam, Jeong-Seok;Jung, Ji-Youn
    • Journal of Food Hygiene and Safety
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    • v.24 no.3
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    • pp.267-272
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    • 2009
  • The trace element nutrient selenium discharges its well-known nutritional anti-tumor activity. Converging data from epidemiological, ecological and clinical studies have shown that selenium can decrease the risk for some types of human cancers, especially those of the prostate, lung, and colon. Mechanistic studies have indicated that selenium has many desirable attributes of chemoprevention targeting cancer cells through DNA single strand breaks, the induction of reactive oxygen species. However, there is no reports about the relationship between methylseleninic acid (MSeA), one of methylselenol metabolites and cell cycle arrest in LNCaP human prostate cancer cells. Our data showed that MSeA arrested G1/S pahse of cell cycle arrest and inhibited DNA synthesis in LNCaP cells and those cellular events by MSeA were due to the induction ofp27 protein which is a well-known cyclin-dependent kinase inhibitor. Taken together, cell cycle arrest occurred by MSeA may contribute to the growth-inhibition of prostate cancer cells.

Throughput Improvement and Power-Interruption Consideration of Fly-By-Wire Flight Control Computer (비행제어 컴퓨터의 Throughput 향상 및 Power-Interuption 대처 설계)

  • Lee, Cheol;Seo, Joon-Ho;Ham, Heung-Bin;Cho, In-Je;Woon, Hyung-Sik
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.35 no.10
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    • pp.940-947
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    • 2007
  • For the performance upgrade of a supersonic jet fighter, the processor and FLCC(Flight Control Computer) Architecture were upgraded from a baseline FLCC. Prior to the hardware implementation phase, the exact CPU throughput estimation is necessary. For this purpose, an experimental method for new FLCC throughput estimation was introduced in this study. While baseline FLCC operating, the CPU address bus was collected with logic analyzer, and then decoded to get the exact access times to each memory-memory and the number of program Instruction branches. Based on these data, a throughput test in CPU demo-board of the new FLCC configuration was performed. From test results, the CPU-Memory architecture was design-changed before FLCC hardware implementation phase. To check the flight stability degradation due to power-interrupt problem due to CPU-Memory architecture change, the piloted HILS (Hardware-In-the Loop Simulator) test was conducted.

Production and Properties of Tannase from Lenzites betulina (Lenzites betulina에 의한 Tannase 생산 및 성질에 관한 연구)

  • Hong, Jae-Sik;Kim, Myung-Kon;Kim, Keum-Jae;Kwak, In-Gu;Yoon, Sook
    • Microbiology and Biotechnology Letters
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    • v.18 no.6
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    • pp.591-598
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    • 1990
  • Six species under the basidiomycetes were screened for extracellular tannase (tannin acyl hydrolase EC 3.1. 1.20) production in submerged culture and Lenzites betulina was found to be most effective for the production of tannase. The optimum cultural conditions for tannase production were $25^{\circ}C$, pH 6.0 and 21 days of culture period, The efficient composition of culture medium for the production of tannase was performed in synthetic medium containing tannic acid, 2g; sucrose, 5g; bacto-peptone, 2g; ,$ KH_2PO_4, \;2g,\; MgSO_4.7H_2O \;0.5g,\; CuS0_4.5H_2O$, 2 mg; thiamine HCl, 100 ug and distilled water 100 ml, The tannase produced from Lenzites bdulin*r was 223.3 unit (umole of gaUic acidiml of brothlmin). The tannase had an optimal reaction conditions ofpH 6.0 and temperature of $40^{\circ}C$. The enzyme was stable at temperature below $40^{\circ}C$ and lost its activity by 50% above $60^{\circ}C$. And the stable pH range was 5.5 to 6.0.

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Survey of Plant Parasitic Nematodes on Economic Crops (경제작물(經濟作物) 주산단지(主産團地) 선충 발생상황(發生狀況) 조사(調査))

  • Cho, H.J.;Han, S.C.
    • Korean journal of applied entomology
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    • v.25 no.3 s.68
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    • pp.175-182
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    • 1986
  • A study was conducted to survey the fauna and the field density ofp lant parastic nematodes on important cash crops in Korea. Studies were carried out with sampling about five hundred grams of soil around roots of eighteen different kinds of crops from fifty five locations throughout the country. Nematodes were elutriated from over three thousand soil samples, and identified into sixteen different genera. Among them Ditylenchus species were found to be the most abundant in fields of garlic and onion, Helicoty-lenchus of sesame, chinese cabbage and mulberry, Meloidogyne of red-pepper, tomato, cucumber and peanut, Pratylenchus of mulberry and peach, Pratylenchus of apple, Chinese cabbage and radish, Trichodorus of potato, and Xiphinema of peach and potato. Field density of Meloidogyne species was increased with the longer continuous cultivation of red-pepper in the same field.

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Implementation of Vertigo Warning function for FA-50 aircraft

  • You, Eun-Kyung;Kim, Hyeock-Jin
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.10
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    • pp.1-9
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    • 2019
  • Fighter pilots are taking 'Advanced Pilot Training' courses to perform their missions perfectly even under adverse conditions. However, there are accidents that fall due to problems with the human body's equilibrium in the acceleration of flight, falling into the 'Vertigo, Spatial disorientation' phenomenon. As such, accidents that fighters fall due to spatial disorientation frequently occur not only in Korea but also abroad. In this study, we implemented the 'Vertigo' warning function in the fighter. First, we analyzed the aircraft's mission computer and the currently implemented warning functions. And we studied the coordinate system to utilize the aircraft attitude information. Based on this, we wanted to provide a visual warning to the HUD when the fighter flies over a certain time in the inverted flight position. Implementing this feature is expected to improve pilot flight safety. In addition, based on the results of this study, we propose a method to implement warning functions through linkage with other subsystems.