• The Plant Pathology Journal
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• v.24 no.3
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• pp.309-316
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• 2008

Mycelium of Ophiostoma quercus albino strain cultured in liquid culture media was harvested, lyophilized, and stored for examining biocontrol efficacy against wood discoloration by staining fungi in the laboratory and field conditions. Dry weight of mycelium grown in brown sugar yeast extract broth(BYB) showed 3.8 times higher than that grown in potato dextrose broth(PDB). The optimum culture period in BYB was 4 weeks. In vitality test of the albino strain, the lyophilized mycelium stored in liquid nitrogen($-196^{\circ}C$) or in a refrigerator($4^{\circ}C$) kept the vitality until 13 months after storage; however, the mycelium stored at room temperature lost the vitality completely after 13 months. The mycelium stored in liquid nitrogen or in a refrigerator protected wood chips from the discoloration by pretreating mycelial suspension on pine wood chips. The mycelium stored at room temperature for 7 months also showed complete protection. These results suggest that the lyophilized mycelium have a biocontrol efficacy only if it keeps the least vitality. In the field conditions, both albino strain and $Woodguard^{(R)}$(commercial chemical protectant) showed significant differences(p=0.05) in discoloration rate as compared to the non-treated control when these were treated on the wood logs of Pinus rigida. The albino strain showed better protection than $Woodguard^{(R)}$. Isolation frequency of blue stain fungi from the chips of wood logs treated with the albino strain was 0% at three months after treatment, while that treated with $Woodguard^{(R)}$ was 76.7%. In another experiment, pre-treatment of mycelial suspension on the cut surface of wood logs also showed significant protection from wood discoloration. Spraying of both albino strain on the cut surface and insecticides on the bark also showed relatively good control effects as compared to insecticide alone on the bark or nontreated control.

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2010.10a
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• pp.46-50
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• 2010

• Journal of the Korean Wood Science and Technology
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• v.43 no.2
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• pp.224-237
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• 2015

We hypothesized that the extract from steam exploded Q. variabilis might be cytoprotective for tenofibroblasts cells during oxidative stress. In the present study, the extracts obtained from steam exploded (severity log Ro 4.68) Q. variabilis contained high quantities of phenolics and flavonoids contents. Also, the extracts appear to have, on these tenofibroblasts, a protective effect against oxidative stress. Tenofibroblasts cells incubated with the extracts and stressed with $H_2O_2$ showed an increase in cell viability by MTT assay. The extracts is found to inhibit $H_2O_2$-induced apoptosis in tenofibroblasts cells, as shown by Annexin V/PI double staining analysis. Western blot data showed that in the extracts/$H_2O_2$-treated cells, the extracts inhibited the $H_2O_2$-dependent phosphorylation of ERK and p38. From these results, it is suggested that the extracts showed the protective effect on $H_2O_2$-mediated oxidative stress. The main chemical compounds of the extract was identified as 1,8-cineole by GC-MS analysis. The anti-apoptosis activity is accordingly believed to be attributable to the 1,8-cineole.

• Journal of Ecology and Environment
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• v.34 no.3
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• pp.259-267
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• 2011

A pollen analytical study of sediment sequences collected from Yongneup moor (sampling point: $38^{\circ}$12'57.4" N, $120^{\circ}$7'30.2" E) was conducted to understand the vegetation history in the mountainous region of the central Korean peninsula. Carbon dating was carried out to measure five successive samples obtained from the bottom at a depth of 180 cm to the surface. The Yongneup moor sediment revealed four main local pollen zones; that is, four past vegetation phases as follows: Local pollen zone I: Quercus-Pinus zone; estimated age, 5,900-4,800 calibrated years (cal) before present (BP); vegetation type, cool-temperate central/montane deciduous broad-leaved forest. Local pollen zone II: Pinus-Abies-Quercus zone; estimated age, 4,800-3,400 cal BP; vegetation type, cool-temperate northern/alti-montane mixed coniferous and deciduous broad-leaved forest. Local pollen zone III: Quercus-Pinus-Abies zone; estimated age, 3,400-400 cal BP; vegetation type: cool-temperate central/montane deciduous broad-leaved forest. Local pollen zone IV: Pinus-Quercus zone; estimated age, 400-present cal BP; vegetation type, cool-temperate central/montane mixed deciduous broad-leaved and coniferous forest. It was confirmed that subalpine coniferous forests had expanded to the mountainous region of the central Korean peninsula during the period from 4,800-3,400 cal BP and thereafter deciduous forests dominated by Q. mongolica were established. Notably, secondary forests dominated by P. densiflora developed in the lower part of the mountainous region of the central Korean peninsula about 400 cal BP due to human interference.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.325-337
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• 2019

This study was conducted to analyze character of distribution of plants by landforms and altitude in Mt. myeonbong. The chemical properties of soil in this study sites were as follows; pH 4.86, organic 52.53 g/kg, $P_2O_5$ 7.57 mg/kg, potassium $0.18cmol^+/kg$, calcium $2.45cmol^+/kg$ and magnesium $0.49cmol^+/kg$. Generalists in the upper layer were analyzed as follows; Quercus mongolica, Pinus densiflora, Quercus variabilis, and specialists; Carpinus cordata, Tilia amurensis, Morus bombycis etc. In the middle layer, generalists were Betula schmidtii, Quercus mongolica, Sorbus alnifolia and specialists; Lindera erythrocarpa, Quercus serrata, Staphylea bumalda etc. Generalist of shrubs; Lindera obtusiloba, Rhododendron mucronulatum, Sorbus alnifolia, and specialists; Morus bombycis, Pinus densiflora, Deutzia glabrata etc. Generalists of herbaceous layer; Rhus tricocarpa, Lespedeza maximowiczii, Polygonatum odoratum var. pluriflorum and specialists; Liparis kumokiri, Davallia mariesii and Chrysosplenium flagelliferum etc.

• The Korean Journal of Ecology
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• v.12 no.2
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• pp.109-122
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• 1989

The vegetation and landscape of Hokkaido were phytosociologically referred. The region of F a g e t e a c r e n a t a e on Hokkaido is divided into two types of deciduous broad-leaved forest: the oak (Quercus mongolica var. grosseserrata) forests mixed with conifers (mainly Abies sachalinensis) and the beech (Fagus crenata) forests of northernmost distribution in far-east Asia. The oak forests, which is dominated by Quercus mongolica var. grosseserrata in Japanse islands, seem to be developed from different climatic and edaphic conditions, especially in the amount and sharing pattern of precipitation in a year, and weak acid brown forest soil, volcanic ash soil and sandy soil. On the all-inclusive phytogeographical view-point, Hokkaido is situated at northernmost region of F a g e t e a c r e n a t a e (cool-temperate zone)neighboring with subarctic and subalpine vegetation, vegetation, but the evergreen broad-leaved forest (C a m e l l i e t e a j a p o n i c a e, warm-temperate zone) is abscent.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.120-124
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• 1994

Browse from oriental white oak(Quercus aliena Blume) was analysed for nutritive value and biomass estimation. The content of cmde protein was high on August and September. NDF, ADF and lignin contents were increased with progressing the seasons but IVDMD, TDN, DE and ME levels were not different from the variation of seasons. Tannin content was high on May and June. Basal stem diameter $\times$ height of tress was highly correlated with browse dry weights(r=0.80**) and the linear regression equation are the follows; Y = 8.23 + O.57X(Y = browse dry weight(g), X= basal stem diameter $\times$ height(cm)). Using the traditional cutting method, the amounts of browse was determined as 823 g dry weight per tree, while the new method by the above equation gave a similar results, i. e., 793 g dry weight per tree. The possibility of browse biomass estimation of oriental white oak by the basal stem diameter $\times$ height index was found.

• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.66-73
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• 1985

Factors affecting isolation and fusion of protoplasts of three Quercus species were investigated and procedures for isolation, purification and fusion of protoplasts of the three species were also established. Unhardened leaves and rapidly growing callus cultures were good source of viable protoplasts. The optimum composition of enzyme mixture for rapid isolation of protoplasts from leaf mesophyll tissues and calli was Cellulase Onozuka R-10 (20g/l, Macerozyme R-10(10g/l), Pectinase(250 units/l, $CaCl_2$, $2H_2O$(14mM), $MgSO_4{\cdot}7H_2O$(1.8mM), $KNO_3$(1.0mM), $H_3BO_3$(1.0mM), $KH_2PO_4$(0.2mM), KI($1.0{\mu}M$), 1,4-dithiothreitol (0.1mM) and mannitol (0.6M). Optimum density of protoplasts for maximum fusion was $2{\times}10^5/ml$ which was the highest protoplast density given in this study. Optimum concentration and duration of PEG 1450 treatment for inducing fusion appeared to be 29%(W/V) final PEG 1450 concentration and 5-10 minutes, respectively.

• Journal of Korean Society of Forest Science
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• v.80 no.2
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• pp.210-219
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• 1991

This study is to diagnose the drought tolerance of twenty broad-leaved tree species by the pressure-volume(P-V) curves. As for the diagnosis of drought tolerance, the valuable water relations parameters obtained from P-V curves are the osmotic potential at full turgor, ${\Psi}_0{^{sat}}$, osmotic potential at incipient plasmolysis, ${\Psi}_0{^{tlp}}$, maximum bulk modulus of elasticity, $E_{max}$, and relative water content at incipient plasmolysis, $RWC^{tlp}$. Also, the figures related to the diagnosis of drought tolerance are the free water content (FWC) versus leaf water potential(${\Psi}_L$), volume-averaged turgor pressure ($P_{vat}$) versus leaf water potential (${\Psi}_L$), and H$\ddot{o}$fler diagram. In this study, the relatively high drought tolerant species are Fraxinus rhynchophylla, Quercus acutissima, Quercus serrata, Quercus aliena, and Populus alba${\times}$glandulosa ; the relatively low drought tolerant species are Fraxinus mandshurica, Betula platyphylla var. japonica, Populus euramericana, Kalopanax pictum, Carpinus loxiflora, Carpinus cordata, Prunus sargentii, Prunus leveilleana, and Cornus controversa ; medium species are Quercus mongolica, Acer mono, Acer triflorum, Acer pseudo-sieboldianum, Ulmus davidiana, and Zelkova serrata.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.266-273
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• 2008

The effects of soil acidification on the seedling growth and nutrition of Pinus densiflora and Quercus acutissima were investigated. The relationship between the seedling growth and molar (Ca+Mg+K)/Al ratio of in soil solution was examined. The results suggested that growth inhibition of seedling Pinus densiflora and Quercus acutissima was due to the low pH of soil solution, which was followed by leach of Al into soil solution, and decrease of essential elements, such as Ca in aerial pant of the seeding caused by the increase of Al concentration in subterranean pant of the seedlings. The level of growth inhibition was determined not only by Al concentration, but also by the balance of inorganic elements, Al, Ca, Mg and K. The growths of two species in total dry weight were clearly inhibited when molar (Ca+Mg+K)/Al ratio of the soil was lower than 6.0. The growth in dry weight, in the condition of the molar ratio was 0.8, was decreased 60% or 50% for the seedling of Pinus densiflora or Quercus acutissima respectively. It was concluded that the molar (Ca+Mg+K)/ Al ratio could be an important index for evaluation of the effects of soil acidification, due to acid deposition such as acid rain, on growth of trees and nutrition. And it might be a more useful indicator for evaluation of critical load of acid deposition on forest ecosystems.