• Restorative Dentistry and Endodontics
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• v.20 no.2
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• pp.645-654
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• 1995

Porphyromonas endodontalis is a black-pigmented anaerobic Gram negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. DNA probe is an available alternative, offering the direct detection of a specific microorganism. Nucleic-acid probes can be off different types: whole different: whole-genomic, cloned or oligonucleotide probes. Wholegenomic probes are the most sensitive because the entire genome is used for possible hybridization sites. However, as genetically similar species of bacteria are likely to be present in specimences, cross-reactions need to be considered. Cloned probes are isolated sequences of DNA that do not show cross-reactivity and are produced in quantity by cloning in a plasmid vector. Cloned probes can approach the sensitivity found with whole-genomic probes while avoiding known cross-reacting species. Porphyromonas endodontalis ATCC 35406 (serotype $O_1K_1$) was selected in this experiment to develop specific cloned DNA probes. EcoR I-digested genomic DNA fragments of P. endodontalis ATCC 35406 were cloned into pUC18 plasmid vector. From the E. coli transformed with the recombinant plasmid 4 clones were selected to be tested as specific DNA probes. Restriction-digested whole-genomic DNAs prepared from P. gingivalis 38(serotype a), W50(serotype b), A7A1-28(serotype C), P. intermedia 9336(serotype b), G8-9K-3(serotype C), P. endodontalis ATCC 35406(serotype $O_1K_1$), A. a Y4(serotype b), 75(serotype a), 67(serotype c), were each seperated on agarose gel electrophoresis, blotted on nylon membranes, and were hybridized with digoxigenin-dUTP labeled probe. The results were as follows: 1. Three clones of 1.6kb(probe e), 1.6kb(probe f), and 0.9kb(probe h) in size, were obtained. These clones were identified to be a part of the genomic DNA of P. endodontalis ATCC 35406 judging from their specific hybridization to the genomic DNA fragments of their own size on Southern blot. 2. The clones of 4.9kb(probe i) was identified to be a part of the genomic DNA of P. endodontalis ATCC 35406. but not to specific for itself. It was hybridized to P. gingivalis A7A1-28, P. intermedia G89K-3.

• Archives of Plastic Surgery
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• v.37 no.6
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• pp.721-725
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• 2010

Purpose: The survival of composite graft is dependent on three steps, (1) plasmatic imbibitions, (2) inosculation, and (3) neovascularization. Among the many trials to increase the survival rate of composite graft, prostaglandin E1 (PGE1) has beneficial effects on the microcirculatory level with vasodilating, antithrombotic, anti-inflammatory and neoangiogenic properties. Lipo-PGE1 which is lipid microspheres containing PGE1 had developed to compensate the systemic and local side effects of PGE1. This study was proposed to determine whether Lipo-PGE1 administration enhanced the survival of composite graft through neovascularization quantitatively in a rabbit ear model. Methods: Fourteen New Zealand White Rabbits each weighing 3~4 kg were divided in two groups: (1) intravenous Lipo-PGE1 injection group and (2) control group. A $2{\times}1\;cm$ sized, full-thickness rectangular composite graft was harvested in each auricle. Then, the graft was reaaproximated in situ using a 5-0 nylon suture. For the experimental group, $3{\mu}g$/kg/day of Lipo-PGE1 ($5{\mu}g$/mL) was administered intravenously through the marginal vein of the ear for 14 days. The control group was received no pharmacologic treatment. On the 14th postoperative day, composite graft of the ear was harvested and immunochemistry staining used Monoclonal mouse anti-CD 31 antibody was performed. Neoangiogenesis was quantified by counting the vessels that showed luminal structures surrounded by the brown color-stained epithelium and counted from 10 random high-power fields (400x) by independent blinded observer. Statistical analysis (Wilcoxon Signed Ranks test for nonparametric data) was performed using SPSS v12.0, with values of p<0.05 considered significant. Results: The mean number of the microvessels was $15.48{\pm}8.65$ in the experimental group and $9.82{\pm}7.25$ in the control group (p=0.028). Conclusion: The use of Lipo-PGE1 facilitated the neoangiogenesis, resulted in the improvement of the survival rate of graft. On the basis of this results, we could support wider application of Lipo-PGE1 for more effective therapeutic angiogenesis and successful survival in various cases of composite graft in the human.

• Journal of Chest Surgery
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• v.31 no.5
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• pp.531-535
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• 1998

In 1964, Abbott and Colleagues published the world's first heterotopic heart transplantation technique in the rat. Their method established circulation by end-to-end anastomoses of the graft's aorta and pulmonary artery to the recipient's abdominal aorta and Inferior Vena Cava(IVC), respectively. In 1966, Tomita et al altered Abbott's technique by employing end-to-side rather than end-to-end anastomoses, thus eliminating the hind leg paralysis that sometimes resulted from Abbott's technique. In order to prevent postsuture hemorrhage (since 7-0 silk suture was the finest available at that time), Tomita's aortic anastomosis was done with double up-and-down continuous suture technique. A single layer continuous anstomosis effected the pulmonary artery-IVC anastomosis. The availability of Nylon monofilament suture made it possible for Ono and Lindsey to use a single layer suture technique for the aortic end-to-side anastomosis in their modified rat heart transplantation. We observed survival time between control group and Immunosuppression(Cyclosporine administration, 10mg/Kg${\times}$4 times postoperatively) group after heterotopic heart transplantation in the rat model. The cyclosporine adminstration group survived longer than the control group, thus we concluded that cyclosporine was based on Immunosuppressive drugs.

• Journal of Wetlands Research
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• v.17 no.2
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• pp.203-208
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• 2015

To identify the effect of fallen cherry blossom on the artificial pond ecosystem, microcosm experiment was conducted into the aquatic decomposition of Prunus species petals. Petals were put in $1mm^2$ mesh nylon litter bags. For treatment group, one flower litter bag was placed into each pot microcosm ($27{\times}20{\times}8cm^3$) filled with influent water from the artificial pond, whereas control group microcosm contained pond water only. Decomposition time were set differently (4, 8, 12, 16 days) among treatment groups. At the end of experiment, most petals were decomposed and only 32.3% of initial dry weight remained with the decay rate (k) of $7.06{\times}10^{-2}day^{-1}$. $NO_3-N$ concentration of microcosm water decreased sharply from 1.90 mg/L at first to 0.02 mg/L, whereas $NH_4-N$ concentration increased from 0.03 mg/L to 2.85 mg/L continually. $PO_4-P$ concentration was 0.03 mg/L at first and increased to 2.39 mg/L by decomposition. Therefore, available phosphorus seems to have leached with higher rate than nitrogen from the petals litter. Increase about 0.02 mg/L in $PO_4-P$ concentration could be estimated in artificial pond from the calculation on the total quantity of fallen blossoms. This result suggests that available phosphorus from the decomposed Prunus petals could cause eutrophication in the artificial pond.

• Journal of Veterinary Clinics
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• v.24 no.3
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• pp.384-391
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• 2007

This study was conducted to clarify the effects of the Korean raisin (Hovenia dulcis Thunb) extracts and polyherbal extracts consisted of 6 types herbal extracts including HDT (HDTmix) on $CCl_4$ induced organ damages. Extracts were prepared by autoclave ($121^{\circ}C$, 15 psi, 3 hours) and filtered with nylon cloth and filter paper then freezing dried. Male Sprague-Dawley rats $(200^{\circ}{\ae}20g)$ were used as experimental groups, which were divided into 5 groups; intact control group (100 mg/kg), $CCl_4$ dosing group (100 mg/kg), $CCl_4$ dosing after HDT extract dosing group (100 mg/kg), $CCl_4$ dosing after HDTmix dosing group (100 mg/kg), Silymarin dosing group (5 mg/kg) after all test articles were orally dosed once a day for 28 days. They were sacrifiled under ether anesthesia. HDT extracts and HDTmix dramatically inhibits the $CCl_4$ intoxicated hepato/nephropathies with immuno-suppress changes on the spleen. They showed more dramatical protective effects on most of specific organs compared to that of Silymarin 5 mg/kg except for hepatoprotective effects in which, quite similar effects were detected. In addition, HDT extracts showed synergic effects with other types of herbal extracts because HDTmix showed more favorable protective effects on the all specific organs showing $CCl_4-related$ histopathological changes compared to HDT extracts.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.678-684
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• 2016

The detection of an underwater work space boundary is very important when an underwater construction is carried out using seabed type underwater equipment, such as underwater machines for rubble mound leveling, because it can induce industrial disasters. Therefore, divers are needed to mark the underwater work space boundary. A nylon rope is used to improve the convenience during an underwater diver's work. The results showed that the work space boundary can be detected using a sonar system. Using these results, an efficient method to detect the underwater work space boundary can be obtained when an underwater construction is carried out using seabed type underwater equipment.

• Journal of agriculture & life science
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• v.45 no.1
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• pp.89-99
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• 2011

This study was carried out to investigate effect of irradiation doses on quality of vacuum-packed dry-cured ham during storage. Dry-cured pork slices were vacuum-packaged in nylon/ polyethylene bags. Vacuum-packed dry curd pork was stored at $10{^{\circ}C}$ for 12 weeks after irradiation (0 kGy, 2.5 kGy, 5 kGy, 7.5 kGy and 10 kGy) and physicochemical properties of dry curd porks, such as meat color surface, thiobarbituric aicd reactive substances, volatile basic nitrogen, pH, microbial (total plate count, Lactobacillus) and sensory properties (color, flavor, overall acceptability) were investigated. Irradiation treatment decreased a*-value, TPC and Lactobacillus. Irradiation dose significantly (p<0.05) increased irradiation flavor and TBA value. However, irradiation dose decreased redness and sensory color in dry-cured ham. The quality of dry-cured ham was affected by the irradiation dose. The results suggested that dry-cured ham irradiated at 5 kGy dose may show optimum quality characteristics of the products as well as microbial safety.

• Journal of the Korean GEO-environmental Society
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• v.22 no.11
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• pp.31-37
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• 2021

To prevent accidents due to the cavities and loosened layers formed due to water leakage from the deteriorated buried pipes, evaluation of the changes in water contents around the buried pipes is required. As a method to evaluate the water contents of the soils, time domain reflectometry (TDR) system can be adopted. However, slender electrodes used in standard TDR probe may be damaged when buried in the ground. Thus, in this study, buried type TDR module was developed for the evaluation of the water contents with maintaining required shape of the electrodes in the ground. The TDR module is composed of three electrodes connected to the core conductor and outer conductor and a casing to prevent deformation and maintain alignment of the electrodes in the ground. For the verification of TDR waveforms measured using the TDR module, comparative analysis was conducted with the TDR waveforms measured using the standard TDR probe, and the relationship between the volumetric water content of the soils and the travel time of the guided electromagnetic wave was constructed. In addition, a model test was conducted to test the applicability of the buried type TDR module, and the experimental result shows that the TDR module clearly evaluates the changes in volumetric water contents due to the leakage from the modeled buried pipe. Therefore, the buried type TDR module may be effectively used for the health monitoring of the buried pipe and the evaluation of the water contents around the pipes buried in the urban pavements.

• Journal of Korean Society of Spine Surgery
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• v.25 no.4
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• pp.180-184
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• 2018

Study Design: Case report Objectives: To document fistula formation between the disc and dura by an unrecognized dural tear after percutaneous endoscopic lumbar discectomy (PELD). Summary of Literature Review: The risk of durotomy is relatively low with PELD, but cases of unrecognized durotomies have been reported. An effective diagnostic tool for such situations has not yet been identified. Materials and Methods: A patient twice underwent transforaminal PELD under the diagnosis of a herniated lumbar disc at L4-5. She still complained of intractable pain and motor weakness around the left lower extremity at 6 months postoperatively. Magnetic resonance imaging showed no specific findings suggestive of violation of the nerve root. However, L5 and S1 nerve root injury was noted on electromyography. An exploratory operation was planned to characterize damage to the neural structures. Results: In the exploration, a dural tear was found at the previous operative site, along with a fistula between the disc and dura was also found at the dural tear site. The durotomy site was located on the ventrolateral side of the dura and measured approximately 5 mm. The durotomy site was repaired with Nylon 5-0 and adhesive sealants. The patient's preoperative symptoms diminished considerably. Conclusions: Fistula formation between the disc and dura can be caused by an unrecognized dural tear after PELD. Discography is a reliable diagnostic tool for fistulas formed by an unrecognized durotomy.

• Fire Science and Engineering
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• v.32 no.6
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• pp.74-83
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• 2018

In order to secure safety and improve work efficiency of forest firefighting crews, performance evaluation criteria for safety gloves were developed. Since the standard for forest firefighting safety gloves is not available in Korea, we started the study based on the standard for firefighting safety gloves operated by KFI. 12 test items were selected excluding 5 test items out of the 17 test items through the consultation of stakeholders and the review of foreign standards. Two types of general safety gloves used by forest firefighting crews and one firefighting gloves were tested by applying KFI standard and the results were compared. Materials such as nylon or polyurethane which are weak to fire are not suitable for forest firefighting safety gloves. We compared the criteria of KFI, ISO 16073, NFPA 1977, NFPA 1971 and BS EN 659, and finally selected 12 test items and acceptance criteria suitable for the work environment of forest firefighting crews in Korea.