• Title/Summary/Keyword: Nutrient solution culture

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Management of nutrient solution based on $NH_4H_2PO_$ concentration in deep flow culture of cherry tomato (방울토마토 담액수경재배시 $NH_4H_2PO_$ 농도에 기초한 배양액 조절)

  • 이문정;김성은;김영식
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1995.10a
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    • pp.89-92
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    • 1995
  • 순환식 수경재배에서 배양액의 조성이나 농도를 정해놓고 일정기간 공급하면 배양액의 pH와 EC의 변화가 생긴다. 보통 배양액의 공급은 재배개시시에 다량 공급하고 그 이후에는 부족한 양만큼을 공급하게 되는데, 시작배양액과 추가배양액의 조성은 같아도 이온의 총량은 다르다. 때문에 재배기간중에 pH의 변화양상이 다르게 나타나는 것이다. 이러한 현상을 피하기 위하여 이온의 총량을 기초로 하여 추가배양액의 이온조성을 조정하여 공급하므로써 배양액의 pH를 안정하게 유지할 수 있을 것으로 추측한다. (중략)

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Effect of Inorganic Nitrogen on Photorespiration of Pea Leaves (완두잎의 광호흡에 미치는 무기질소의 영향)

  • 이인철
    • Journal of Plant Biology
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    • v.27 no.4
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    • pp.253-261
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    • 1984
  • Leaf discs isolated from the pea seedling grown in nutrient solution containing 5mM ammonia or nitrate exhibited a half level of photorespiration as compared with the nitrogen free control. The manifestation of the ammonia effect appeared somewhat earlier than that of nitrate effect, but this difference subsided as the culture periods was extended. The total amount of ${CO}_2$ fixed by leaves from nitrogen-supplemented seedlings showed approximately 1.5 fold increase over the control with the ammonia effect being manifested earlier than the nitrate effect. The activities of peroxisomal serine: glyoxylate aminotransferase were always higher with ammonia than nitrate, the two types of nitrogen source, however, had similar effect on conversion rate of glyoxylate into glycine. These results indicate that exogenous ammonia does not act directly as an effector of this aminotransferase in vivo. But changes in the level of the pool size of glycine and serine, both of which are the intermediates of photorespiratory process, suggest that exogenous ammonia inhibit the transformation of serine from glycine metabolically.

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Increased Poly(3-Hydroxybutyrate) Accumulation in Recombinant Escherichia coli from Whey by Agitation Speed Control

  • Kim, Beom-Soo;Brian K. O'Neill;Lee, Sang-Yup
    • Journal of Microbiology and Biotechnology
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    • v.10 no.5
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    • pp.628-631
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    • 2000
  • The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

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Effect of $\textrm{NO}_3$:$\textrm{NH}_4$ Ratio on the $\textrm{NO}_3$ Content in Leaf Lettuce Cultured by a Deep Flow Technique (잎상추의 수경재배시 $\textrm{NO}_3$-N과 $\textrm{NH}_4$-N의 비율이 엽중 nitrate 함량에 미치는 효과)

  • 김혜진;김영식
    • Journal of Bio-Environment Control
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    • v.10 no.1
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    • pp.50-54
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    • 2001
  • Effect of NO$_3$:NH$_4$ratio and pH of nutrient solution on nitrate content of leaves was investigated for leaf lettuce(Lactuca sativa L.) grown by a deep flow technique. Ratios(in me.L$^{-1}$ ) of NO$_3$:NH$_4$tested were 12:1, 10:3 and 8:5. The treatment of 8:5 NO$_3$:NH$_4$ratio had two solutions, one with uncontrolled pH and the other with automatically controlled pH. Solution pH continuously increased in 12:1 NO$_3$:NH$_4$treatment. Solution pH decreased gradually more as NH$_4$ratio increased. Treatment of 8:5 NO$_3$:NH$_4$with automatic pH control satisfactorily maintained the solution pH in the range of pH 5.5-6.0. Nitrate content in leaves was the greatest in treatment of 12:1 NO$_3$:NH$_4$ and the least in treatment of 8:5 NO$_3$:NH$_4$with automatic pH control. Fresh weight decreased in treatments of 10:3 and 8:5 NO$_3$:NH$_4$, whereas it increased in treatments of 12:1 and 8:5 NO$_3$:NH$_4$with pH control. It was concluded that the growth and leaf nitrate content were the greatest in high NH$_4$treatment with automatic pH control.

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Studies on the Nutritional Physiology of Soybean 6. Variatio of Potassium at the Various Position of Leaf on the Main Stem (대두의 영양생리학적 연구 6. 엽위별 가리의 변이)

  • 이순희
    • Journal of Plant Biology
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    • v.17 no.3
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    • pp.127-136
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    • 1974
  • The effect of potassium metabolism on the soybean leaves was studied with comparison of other elements during the successive growing period. The results were as follows; 1. The percentage of potassium content showed remarkable increase not only in the first compound leaf at a stage which was growing vigorously and producing new leaves, but also in the fifth compound leaf at a stage which was taking a active metabolism of nitrogen and carbohydrate but not producing new leaves. However, the percentage of potassium content was decreased in the second compound leaf than in the first one. Such a result could be regarded as a potassium removal from mature leaves into immature and flowing out from stoma through respiration. During the pod-development the percentage of potassium content in the soybean leaf was decreased. 2. If nitrogen, phosphorus and potassium were added excessively in the nutrient solution, the percentage of potassium content in the soybean leaf had increased. The effects of these elements showed a remakable increase in the excessive plot of nitrogen than in that of phosphorus. At early stage the redtarded effect of phosphorus on the growth of soybean could be covered by potassium, however, at late stage it could not. The growth of soybean plant was much more inhibited by potassium, compared with nitrogen and phosphorus. New leaves could not be produced in the potassium deficient soybean plant after the third compound leaf. The normal growth of soybean plant could not be observed if only one element was excessively added to the culture solution, compared with the deficiency of other two elements.

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Equipment Development for Inorganic-Compound Concentration Measurement in a Hydroponic Culture Solution (수경배양액 무기성분농도 측정장치 개발)

  • Heo, Jeong-Wook;Park, Kyeong-Hun;Hong, Seung-Gil;Lee, Jae-Su;Baek, Jeong-Hyun;Park, Jong-Taek;Lee, Seung-Kee
    • Korean Journal of Environmental Agriculture
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    • v.39 no.4
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    • pp.319-326
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    • 2020
  • BACKGROUND: Measurement equipment was developed for inorganic nutrient concentration inside the hydroponic culture medium with several macro- and micro compositions, and applied for measuring the compositions of conventional medium. METHODS AND RESULTS: Before the equipment development, sonicator and heater were utilized to control temperature around of the module mixing with color reagents and target samples among the inorganic compositions. The measurement module and multi-sampler were also manufactured based on the COMS (Complementary Metal-Oxide Semiconductor) and installed inside the measurement equipment. Concentration of standard solution, value measured by the equipment, standard deviation or measured average value were used for estimating the accuracy and average recall of the equipment. Yamazaki solutions with EC of 0.5, 1.5, and 2.5 dS/m were offered to confirm the equipment accuracy and standard error. CONCLUSION: It was suggested that the developed equipment could be automatically applied for measurement with accuracy of over 96% and standard errors of less than 5% on 12 macro- and micro compositions such as a NO3-N, PO43- or Fe.

Effect of Potassium on Corn(Zea mays L.) Seedling Growth under Nutrient Solution Culture (양액재배시(養液栽培時) 칼륨 농도(濃度)가 옥수수(Zea mays L.) 생육(生育)에 미치는 영향(影響))

  • Oh, Jong-Seo;Kim, Moo-Sung;Kim, Se-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.29 no.1
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    • pp.26-33
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    • 1996
  • A nutrient solution culture experiment was conducted in the greenhouse at Suwon Campus. Kyung-Hee University from May 23 to July 11. 1995, to determine the effects of potassium application on seedling growth of Kwanganok and Hwengsungok cultivars of corn. Potassium was treated with four different levels, which are 0.248.496 and 744ppm for treatments without potassium (-k), standard potassium concentration (K), double(2k) and triple (3k) of standard potassium concentration, respectively. Plants were harvested at weekly interval for seven weeks. at which time plant height, leaf area and dry matter yield of corn seedling were measured, only chlorophyll content was taken for five weeks from three week after planting. Seedlings growth of Kwanganok and Hwengsungok cultivars was greatly retarded from planting to harvesting time in 3k treatment. The plant height, leaf area and dry matter yield of both cultivars during the whole period were decreased in order of K>2F>-K>3K, but dry matter yield of Kwanganok cultivar was decreased in order of 2K>K>-K>3K. Chlorophyll content of Kwanganok and Hwengsungok cultivars was irregularly fluctuated during growing period. but generally highest in K treatment and lowest in 3k treatment. The crop growth rates of seedlings of both cultivars were slow until the 3rd week after planting. but very fast thereafter. In general, CGR of both cultivars was higher in K and 2k treatments than -k and 3k treatments.

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The Effect of $\textrm{NO}_3$-N and $\textrm{NH}_4$-N Ratio on the Growth of Perilla frutescens in Hydroponics (수경재배시 $\textrm{NO}_3$-N과 $\textrm{NH}_4$-N의 비율이 잎들깨의 생육에 미치는 영향)

  • 김영식
    • Journal of Bio-Environment Control
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    • v.2 no.2
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    • pp.119-125
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    • 1993
  • The effect of NO$_3$-N and NH$_4$-N ratio on the growth of Perilla frutescens in deep flow culture was studied in winter season. NO$_3$-N and NH$_4$-N were treated in the ratios of 12 : 0, 9 : 3, 6 : 6(me/ $\ell$ ). The pH of the nutrient solution was increased an NO$_3$-N:NH$_4$-N=12 : 0 treatment, and decreased in the treatments containing NH$_4$-N, greatly in NO$_3$-N : NH$_4$-N = 6 : 6 treatment. The EC was increased regardless of treatments, but more increased in the treatments containing NH$_4$-N. The stem-base circumference, plant height, root weight, shoot weight, and yields of leaves were by far the highest in NO$_3$-N:NH$_4$-N=6 : 6 treatment and the lowest in NO$_3$-N:NH$_4$-N= 12 : 0 treatment. Among the mineral contents of leaves, N, K, Fe and P were higher in the treatments containing NH$_4$-N. Ca, Mg and Mn were higher in NO$_3$-N :NH$_4$-N= 12 : 0 treatment.

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Effect of phosphorus on Corn (Zea mays L.) Seedling Growth under Nutrient Solution Culture (양액재배(養液栽培)에서 인산시비(燐酸施肥)가 옥수수(Zea mays L.) 생육(生育)에 미치는 영향(影響))

  • Kim, Moo-Sung;Oh, Jong-Seo
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.4
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    • pp.284-289
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    • 1994
  • A nutrient solution culture experiment was conducted in the greenhouse of Suwon Campus, Kyung-Hee University from May 23 to July 11, 1994, to determine the effects of phosphorus application on seedling growth of Kwanganok and Hwengsungok cultivars of corn. Plants were harvested at weekly interval for seven weeks, at which time plant height, leaf area and dry matter yield of corn seedling were measured. The major results obtained are as follows. In without phosphorus pot, seedlings of Kwanganok and Hwengsungok cultivars have hardly been growing from planting to harvesting time. Plant height, leaf area and dry matter yield of both cultivars were increased more in the seedlings grown under complete treatment compared with the other phosphorus treatments. The total plant height, leaf area and dry matter yield during the whole period of Kwanganok and Hwengsungok cultivars were decreased in order of C>2P>3P>-P application pot. The crop growth rates of seedlings of both cultivars with in all treatments were slow until the 4th week after planting. Kwanganok with 2P and 3P treatment pot and C pot of Hwengsungok reached the maximum CGR at the 5th to 6th week after planting and showed declined CGR's thereafter, but Kwanganok C and Hwengsungok 2P treatment pots maintained a steady increase in CGR's until the end of the experiment period.

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