• Molecules and Cells
• /
• 제45권9호
• /
• pp.649-659
• /
• 2022

A long-term energy nutritional imbalance fundamentally causes the development of obesity and associated fat accumulation. Lysosomes, as nutrient-sensing and lipophagy centers, critically control cellular lipid catabolism in response to nutrient deprivation. However, whether lysosome activity is directly involved in nutrient-induced fat accumulation remains unclear. In this study, worm fat accumulation was induced by 1 mM glucose or 0.02 mM palmitic acid supplementation. Along with the elevation of fat accumulation, lysosomal number and acidification were also increased, suggesting that lysosome activity might be correlated with nutrient-induced fat deposition in Caenorhabditis elegans. Furthermore, treatments with the lysosomal inhibitors chloroquine and leupeptin significantly reduced basal and nutrient-induced fat accumulation in C. elegans. The knockdown of hlh-30, which is a critical gene in lysosomal biogenesis, also resulted in worm fat loss. Finally, the mutation of aak-2, daf-15, and rsks-1 showed that mTORC1 (mechanistic target of rapamycin complex-1) signaling mediated the effects of lysosomes on basal and nutrient-induced fat accumulation in C. elegans. Overall, this study reveals the previously undescribed role of lysosomes in overnutrition sensing, suggesting a new strategy for controlling body fat accumulation.

• 한국토양비료학회지
• /
• 제47권1호
• /
• pp.1-7
• /
• 2014

The objective of this study was to determine the effect of single and co-inoculation of plant growth promoting bacteria (PGPB) on early plant growth in Saemangeum reclaimed soil. Plant growth promoting Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 were inoculated on maize (Zea mays L.) and sorghum-sudangrass hybrid (Sorghum bicolor L.) grown in Saemangeum reclaimed soil. Single and co-inoculation of B. iodinum RS16 and M. oryzae CBMB20 increased plant height, dry biomass accumulation and macro-nutrient accumulation of maize and sorghum-sudangrass hybrid. M. oryzae CBMB20 treatment increased plant height in maize by 41.2% at 30 days after sowing (DAS), shoot dry weight and total dry weight compared to non-inoculated treatment. Macro-nutrient accumulation (N and P) in maize roots was significantly increased with co-inoculation treatment, K and Ca content was significantly increased at B. iodinum RS16 treatment compared to non-inoculated treatment. Macro-nutrient accumulation (P, K, Ca and Mg) in shoot was higher with M. oryzae CBMB20 treatment compared to non-inoculated treatment. In case of sorghum-sudangrass hybrid, co-inoculation treatment showed 33.7% increase in plant height compared to non-inoculated treatment at 30 DAS. M. oryzae CBMB20 treatment increased root dry weight and total dry weight, macro-nutrient accumulation in roots and N, Ca and Mg accumulation in shoot compared to non-inoculated treatment. P and K accumulation in shoot was significantly increased at co-inoculation treatment compared to non-inoculated treatment. This pot culture experiment demonstrated that single and co-inoculation of B. iodinum RS16 and M. oryzae CBMB20 increased the early growth and nutrient accumulation of maize and sorghum-sudangrass hybrid.

• 한국환경복원기술학회지
• /
• 제11권5호
• /
• pp.1-11
• /
• 2008

This study was carried out to provide primary data set for cleaning of contamination site. By having hydroponic culture on Salix alba and S. caprea seedling treated with Ni solution, the result of Ni accumulation came out as followings : In a treatment of Ni to the Salix alba and S. caprea, Ni accumulation increased in its root, leaves, and stem, as Ni concentration became higher until $10.0{\mu}mol$. But in a $100.0{\mu}mol$ treatment, the seedlings died after 4 weeks. Ni accumulation in the Salix alba and S. caprea was the highest in its roots, second-highest in leaves, and the lowest in stems. In the case of $10.0{\mu}mol$ treatment of Ni solution, Ni accumulation in roots were above 500.0mg/L, and leaves were above 20.0mg/L. But it was lower than 13.0mg/L in stems. Ni accumulation in the plant increased more when nutrient solution containing Ni was weekly changed than just refilling the same amount of nutrient solution that evaporated Ni accumulation in Salix alba was higher than S. caprea when the nutrient solution had been refilled only.

• 한국습지학회지
• /
• 제7권4호
• /
• pp.33-42
• /
• 2005

Wetlands often function as a nutrient sink. It is well known that increased input of nutrient increases the primary productivity but it is not well understood what is the fate of produced biomass in wetland ecosystem. Water and sediment quality, decomposition rate of cellulose, and sediment accumulation rate in 11 montane marshes in northern Sierra Nevada, California were analyzed to trace the effect of nitrogen and phosphorus content in water on nutrient dynamics. Concentrations of ammonium, nitrate, soluble reactive phosphorus (SRP) in water were in the range of 27 to 607, 8 to 73, and 6 to 109 ppb, respectively. Concentrations of ammonium, calcium, magnesium, sodium, and potassium in water were the highest in Markleeville, which has been impacted by animal farming. Nitrate and SRP concentrations in water were the highest in Snow Creek, which has been impacted by human residence and a golf course. Cellulose decomposition rates ranged from 4 to 75 % per 90 days and the highest values were measured in Snow Creek. Concentrations of total carbon, nitrogen, and phosphorus in sediment ranged from 8.0 to 42.8, 0.5 to 3.0, and 0.076 to 0.162 %, respectively. Accumulation rates of carbon, nitrogen, and phosphorus fluctuated between 32.7 to 97.1, 2.4 to 9.0, and 0.08 to $1.14gm^{-2}yr{-1}$, respectively. Accumulation rates of carbon and nitrogen were highest in Markleeville and that of phosphorus was highest in Lake Van Norden. Correlation analysis showed that decay rate is correlated with ammonium, nitrate, and SRP in water. There was no correlation between element content in sediment and water quality. Nitrogen accumulation rate was correlated with ammonium in water. These results showed that element accumulation rates in montane wetland ecosystems are determined by decomposition rate rather than nutrient input. This study stresses a need for eco-physiological researches on the response of microbial community to increased nutrient input and environmental change because the microbial community is responsible for the decomposition process.

• BMB Reports
• /
• 제56권2호
• /
• pp.84-89
• /
• 2023

The implications of nutrient starvation due to aging on the degeneration of the retinal pigment epithelium (RPE) is yet to be fully explored. We examined the involvement of AMPK activation in mitochondrial homeostasis and its relationship with the maintenance of a healthy mitochondrial population and epithelial characteristics of RPE cells under nutrient starvation. Nutrient starvation induced mitochondrial senescence, which led to the accumulation of reactive oxygen species (ROS) in RPE cells. As nutrient starvation persisted, RPE cells underwent pathological epithelial-mesenchymal transition (EMT) via the upregulation of TWIST1, a transcription regulator which is activated by ROS-induced NF-κB signaling. Enhanced activation of AMPK with metformin decelerated mitochondrial senescence and EMT progression through mitochondrial biogenesis, primed by activation of PGC1-α. Thus, by facilitating mitochondrial biogenesis, AMPK protects RPE cells from the loss of epithelial integrity due to the accumulation of ROS in senescent mitochondria under nutrient starvation.

• Journal of Microbiology and Biotechnology
• /
• 제3권2호
• /
• pp.115-122
• /
• 1993

Poly(3-hydroxybutyrate)(P(3HB)) accumulation under nutrient-rich condition with various amounts of $(NH_4)_2 SO_4$ was systematically investigated. The results of the electron-microscopy and the solvent extraction showed that the P(3HB) accumulation is unavoidable even under nutrient-rich condition. This indicates that in a two-step culture of Alcaligenes eutrophus H16, the researches should be careful in interpreting the data of polyhydroxyalkanoates(PHAs) accumulation in terms of the carbon-source fed in the second step because the two-step culture product contains the P(3HB) produced under nutrient-rich condition. The polyester production capability in a two-step batch culture of A. eutrophus H16(ATCC 17699) was also investigated using various saccharides and their derivatives such as glucose, fructose, gluconic acid, glucaric acid, sorbitol, lactose, galactose, and mannose. The polyesters synthesized were characterized by 500 MHz$^{1}H-NMR$ spectroscopy, intrinsic viscosity$[\eta]$ measurement in chloroform and differential scanning calorimetry(DSC). 500 MHz $^{1}H-NMR$ analysis showed that all polyesters synthesized generally contained 1~2 mol% of 3HV. Another finding is that the glucose utilization can be increased by changing the autoclaving procedure of the substrate to enhance the P(3HB) production yield up to 46 wt% of P(3HB) in dry cells.

• 한국식품영양과학회지
• /
• 제21권1호
• /
• pp.9-16
• /
• 1992

This study was carried out to evaluate the effect of Acanthopanacis Cortex boiling extract solutions on the fat accumulation in the obese rats induced by the oral high fat administration for six weeks. Total cholesterol, neutral fat and adipose acid of ACR groups were lower than the control group. During the feeding experiment, LDL and VLDL were increased while HDL was decreased in all groups. Insulin and cortisol were higher than the control group, due to the fat accumulation. Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Acanthopanacis Cortex boiling extract solutions.

• 생물환경조절학회지
• /
• 제20권3호
• /
• pp.189-196
• /
• 2011

Ebb and flow 시스템을 사용한 분화 칼랑코에 재배에서 광도와 양액 농도가 생육, 양분 흡수율 및 배지 내 무기 이온의 집적에 미치는 영향을 구명하기 위하여 본 연구를 수행하였다. 연구 목적을 달성하기 위하여 광도 3수준(PPF 4.26, 5.51, $9.75mol{\cdot}m^{-2}{\cdot}d^{-1}$)과 양액 농도 3수준(EC 1.2, 1.8, $2.4dS{\cdot}m^{-1}$)을 조합하여 요인실험으로 수행하였다. 분화 재배한 칼랑코에의 생육은 광도와 양액 농도에 뚜렷하게 영향을 받았으며, 광도가 높아질수록 생육이 양호하였다. 각 광도 내에서는 양액의 EC를 $1.8\sim2.4dS{\cdot}m^{-1}$의 범위로 조절할 경우 칼랑코에 지상부 생육이 비교적 양호하였지만, EC를 $2.4dS{\cdot}m^{-1}$로 조절한 처리의 생체중, 건물중 및 엽면적이 가장 크게 나타났다. 광도와 양액 농도는 ebb and flow 시스템의 양액 탱크로부터 양분 및 수분 흡수량과 배지 내 무기 이온의 집적에 유의하게 영향을 미쳤지만, 식물체의 무기물 함량에는 뚜렷한 영향을 미치지 않았다. 광도는 양액의 EC에 비해 이온의 흡수율에 미치는 영향이 상대적으로 적었으며, 양액 EC가 높을 수록 $NO_3^-$, P, K, Mg 이온의 흡수율은 높았다. 이상의 연구결과를 고려할 때 ebb and flow 저면 관수 시스템에서 배지 내 무기 이온 집적에 영향을 주는 주 요인은 양액 농도임을 확인하였으며, 광도와 양액의 EC를 이용하여 배지 내 무기 이온의 집적량을 예측하기 위한 회귀식을 제시하였다.

• 원예과학기술지
• /
• 제28권6호
• /
• pp.973-979
• /
• 2010

Ebb and flow저면관수 시스템에서 배양액 농도에 따른 칼랑코에(Kalanchoe blossfeldiana 'New Alter')의 생육단계별 생육특성, 양분흡수율 및 배지의 양분집적 상태를 조사하였다. 생육단계별 배양액 농도 처리(EC 0.8, 1.6, 2.4, $3.2dS{\cdot}m^{-1}$)에 따른 작물 생육은 유의한 차이가 있었다. 1, 3, 5, 8, 11주 처리 후에 작물 생육을 조사한 결과, 5주 후부터 처리 별 차이가 매우 컸고, 각 생육단계에서 생장이 가장 양호하였던 처리구는 EC $1.6dS{\cdot}m^{-1}$이었으며, 엽면적, 초장, 건물중은 EC $2.4dS{\cdot}m^{-1}$을 지나 EC $3.2dS{\cdot}m^{-1}$에서 급격히 감소하는 현상을 보였다. 칼랑코에 각 생육단계에서 생장이 가장 양호하였던 EC $1.6dS{\cdot}m^{-1}$처리구에 대해 양분흡수 특성을 조사한 결과 무기이온의 흡수율은 생육단계별 다소 차이가 있었으나 $NO_3$-N가 가장 크고 $Mg^{2+}$가 가장 낮게 나타났다. 배양액 농도 처리구에서 배지 위치 별 EC를 조사한 결과 배지 상층부의 EC는 하층부 EC보다 1-3배 높았고 또한 생육이 진행됨에 따라 배지 상층부 및 하층부에서 집적되는 무기양분의 누적량이 증가되었으며 집적량이 많은 EC $3.2dS{\cdot}m^{-1}$처리구에서는 작물의 생장을 억제시키는 효과가 나타난 것으로 분석되었다. 따라서 EBB저면관수 시스템에서는 배양액의 EC에만 의존할 것이 아니라 배지 내 양분 집적을 고려하여 배양액 공급 시 조성 및 농도를 결정할 필요가 있다.

• Journal of Microbiology and Biotechnology
• /
• 제21권6호
• /
• pp.646-651
• /
• 2011

Cultures of Saccharomyces cerevisiae were treated with pulsed electric fields to improve accumulation of zinc in the biomass. Under optimized conditions, that is, on 15 min exposure of the 20 h grown culture to PEFs of 1500 V and 10 ${\mu}s$ pulse width, accumulation of zinc in the yeast biomass reached a maximum of 15.57 mg/g d.m. Under optimum zinc concentration (100 ${\mu}g$/ml nutrient medium), its accumulation in the cells was higher by 63% in comparison with the control (without PEFs). That accumulation significantly correlated against zinc concentration in the medium. Neither multiple exposure of the cultures to PEFs nor intermittent supplementation of the cultures with zinc increased the zinc accumulation. The intermittent supplementation of the cultures with zinc and multiple exposures on PEFs could even reduce the accumulation efficiency, respectively, by 57% and 47%.