• Title/Summary/Keyword: Nutrient Degradation

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Screening of Organo Phosphorus Insecticide Fenitrothion-Degrading Microorganisms (유기인계 살충제 fenitrothion 분해미생물 탐색)

  • Choi, Hyuek;Kim, Bok-Jin;Bae, Do-Yong;Lee, Young-Deuk;Kang, Sun-Chul
    • Korean Journal of Environmental Agriculture
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    • v.17 no.3
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    • pp.279-285
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    • 1998
  • Fenitrothion-degrading microorganisms were isolated from 124 sampling sites of paddy, upland, forest and polluted soil, and wastewater. A total of 1,071 strains were isolated from each selective medium supplemented with 50mg/l of fenitrothion - nutrient agar (NA) 601, potato dextrose agar (PDA) 201, Actinomycetes isolation agar (AIA) 168 and basal salt medium (BSM) 101, respectively. Twenty-eight effective strains of them, which showed more than 80% degradation of fenitrothion by the gasliquid chromatography(GLC) analysis. were successfully selected from each liquid culture supplemented with 50mg/l of fenitrothion - NB 12(upland soil 3, paddy soil 3, forest soil 2, polluted soil 4), PDB 8(upland soil 1, paddy soil 2, forest soil 2, polluted soil 3) and PSB 8(upland soil 1, forest soil 1, polluted soil 6), respectively. Four strains - NPal, NFol, PFol and BPol, which have the most powerful degradation activity were finally selected among 28 fenitrothion-degrading microorganisms based on the degradation rate at the concentration of 100mg/l fenitrothion in enrichment media.

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Effects of Activated Carbon and Charcoal on the Nutrients Utilization and Ruminal Fermentation Characteristics in Goat (활성탄 및 목탄의 첨가가 산양의 영양소 이용율 및 반추위내 발효성상에 미치는 영향)

  • Cha, Sang-Woo;Lee, Soo-Kee
    • Korean Journal of Agricultural Science
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    • v.32 no.2
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    • pp.197-203
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    • 2005
  • This study was conducted to investigate the effects of the addition of activated charcoal (AC) and oak charcoal on the ruminal fermentation characteristics, nutrient utilization in Korean native goats. Compared to reference diet, digestibility of dry matter, and crude protein in AC and charcoal diets tended to incraese. However, any tendency in ruminal degradation of crude fat was not observed. Ruminal degradation of NDF in AC diets tended to be more increased than that in non-AC diet. Although it was not significant, ADF tended to be increased in AC and charcoal diets. AC and charcoal did not affect the ruminal pH and ammonia-N. Concentration of total VFA and butyrate tended to be increased by adding AC and charcoal(p<0.05). Although it was not significant, acetate/propionate ratio tended to decreased in AC and charcoal diets. Although there appeared some beneficial effects in adding AC and charcoal to ruminant diets in this study, more works could be needed with AC before we can make clear conclusion on use of AC and charcoal in the ruminant diets.

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Effects of Xylanase Supplementation on Growth Performance, Nutrient Digestibility and Non-starch Polysaccharide Degradation in Different Sections of the Gastrointestinal Tract of Broilers Fed Wheat-based Diets

  • Zhang, L.;Xu, J.;Lei, L.;Jiang, Y.;Gao, F.;Zhou, G.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.6
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    • pp.855-861
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    • 2014
  • This experiment was performed to investigate the effects of exogenous xylanase supplementation on performance, nutrient digestibility and the degradation of non-starch polysaccharides (NSP) in different sections of the gastrointestinal tract (GIT) of broilers fed wheat-based diets. A total of 120 7-day-old Arbor Acres broiler chicks were randomly allotted to two wheat-based experimental diets supplemented with 0 or 1.0 g/kg xylanase. Each treatment was composed of 6 replicates with 10 birds each. Diets were given to the birds from 7 to 21 days of age. The results showed that xylanase supplementation did not affect feed intake, but increased body weight gain of broiler at 21 day of age by 5.8% (p<0.05) and improved feed-to-gain ratio by 5.0% (p<0.05). Xylanase significantly increased (p<0.05) ileal digestibilities of crude protein (CP) by 3.5%, starch by 9.3%, soluble NSP by 43.9% and insoluble NSP by 42.2% relative to the control group, respectively. Also, compared with the control treatment, xylanase addition increased (p<0.05) total tract digestibilities of dry matter by 5.7%, CP by 4.1%, starch by 6.3%, soluble NSP by 50.8%, and had a tendency to increase (p = 0.093) insoluble NSP by 19.9%, respectively. The addition of xylanase increased the concentrations of arabinose and xylose in the digesta of gizzard, duodenum, jejunum, and ileum (p<0.05), and the order of their concentration was ileum>jejunum>duodenum>>gizzard> caecum. The supplementation of xylanse increased ileal isomaltriose concentration (p<0.05), but did not affect the concentrations of isomaltose, panose and 1-kestose in the digesta of all GIT sections. These results suggest that supplementation of xylanase to wheat-based diets cuts the arabinoxylan backbone into small fragments (mainly arabinose and xylose) in the ileum, jejunum and duodenum, and enhances digestibilites of nutrients by decreasing digesta viscosity. The release of arabinose and xylose in the small intestine may also be the important contributors to the growth-promoting effect of xylanase in broilers fed wheat-based diets.

Effect of Electrochemical Oxidation Potential on Biofilter for Bacteriological Oxidation of VOCs to $CO_2$

  • Kang Hye-Sun;Lee Jong-Kwang;Kim Moo-Hoon;Park Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.16 no.3
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    • pp.399-407
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    • 2006
  • In this study, an electrical conductive carbon fiber was used as a biofilter matrix to electrochemically improve the biofilter function. A bioreactor system was composed of carbon fiber (anode), titanium ring, porcelain ring, inorganic nutrient reservoir, and VOC reservoir. Electric DC power of 1.5 volt was charged to the carbon fiber anode (CFA) to induce the electrochemical oxidation potential on the biofilter matrix, but not to the carbon fiber (CF). We tested the effects of electrochemical oxidation potential charged to the CFA on the biofilm structure, the bacterial growth, and the activity for metabolic oxidation of VOCs to $CO_2$, According to the SEM image, the biofilm structure developed in the CFA appeared to be greatly different from that in the CF. The bacterial growth, VOCs degradation, and metabolic oxidation of VOCs to $CO_2$ in the CFA were more activated than those in the CF. On the basis of these results, we propose that the biofilm structure can be improved, and the bacterial growth and the bacterial oxidation activity of VOCs can be activated by the electrochemical oxidation potential charged to a biofilter matrix.

The Effects of Xylose Treatment on Rumen Degradability and Nutrient Digestibility of Soybean and Cottonseed Meals

  • Sacakli, P.;Tuncer, S.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.5
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    • pp.655-660
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    • 2006
  • Two trials were conducted to evaluate the effect of xylose treatment on rumen degradability characteristics of DM, OM and CP and in vivo digestibility of DM, OM, CP and crude fiber (CF) of soybean meal (SBM) and cottonseed meal (CSM). In Trial 1, three ruminally cannulated Merino rams were used. Xylose treatments at both levels, 0.5 and 1%, decreased effective degradability of DM, OM and CP of SBM, whereas 0.5 and 1% xylose treatment of CSM did not show any effect on effective degradability of DM, OM and CP. By contrast, maximum potential degradabilities of DM, OM and CP of CSM seemed to be increased by 1% xylose treatment. It was concluded that xylose treatment was effective in protecting SBM proteins from degradation in the rumen, but the same treatment was not so effective for CSM protein. In trial 2, three Merino rams were used. With treatments, DM, OM, CP and CF digestibilities of SBM and CSM were not changed. Crude fiber digestibility was numerically increased by the treatments of 0.5 and 1% xylose of both SBM and CSM compared to untreated SBM and CSM but differences were not significant. In conclusion SBM proteins can be effectively protected from degradation in the rumen by xylose treatment, without negatively affecting in vivo digestibility of protein, whereas xylose treatment appeared to be less effective on protecting of CSM proteins.

Effects of Leachate during Vegetable Waste Composting using Rotary Drum Composter

  • Varma, V. Sudharsan;Kalamdhad, Ajay S.
    • Environmental Engineering Research
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    • v.19 no.1
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    • pp.67-73
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    • 2014
  • In India, disposal of vegetable market waste along with municipal solid waste in landfills or dumpsites is creating much nuisance in terms of odor nuisance, leachate production, and greenhouse gas emission into the atmosphere. Therefore, vegetable waste with high biodegradable and nutrient content is composted in a 550-L batch scale rotary drum composter to study the degradation process and its compost properties for its potential reuse as high quality compost. A total 150 kg of working volume was fixed for composting studies with two different ratios, trial A (6:3:1) of C/N 24 and trial B (8:1:1) of C/N 30, respectively. A maximum of $63.5^{\circ}C$ and $61.2^{\circ}C$ was observed in trials A and B; an average of $55^{\circ}C$ for more than 5 days, which helped in the degradation of organic matter and reduction of total and fecal coliform. The temperature dropped suddenly after the thermophilic stage in trial B, and leachate was observed due to insufficient amount of bulking agent. Mesophilic bacteria dominated during the initial stages of composting, and reduced considerably during the thermophilic stage. During the thermophilic stage, the rise in spore-forming organisms, including spore-forming bacteria, fungi, actinomycetes and streptomycetes, increased and these were predominant until the end of the composting process. By examination, it was observed that moisture and leachate production had adverse effects on the compost parameters with higher loss of micronutrients and heavy metals.

A Study on the Foam Wastewater Treatment and Foam Collection by Inhalation Force at the Outlet of Power Plants (발전소 방류구의 흡입력을 이용한 거품수거 및 거품액 처리 연구)

  • Jang, Heui-Su;Mun, Gyeng-Seok
    • Journal of Korean Society on Water Environment
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    • v.21 no.5
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    • pp.496-499
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    • 2005
  • Power Plant is requested, by environmental bodies and fisherman, to correct the pollution of coastal area due to the outflow of foam from the outlet of the power plants. Foam wastewater cause a lot of environmental problems, expecially in aesthetic points of view. Therefore, It is needed to be collect from the stream into nearby ocean, and the collected foams should be treated before being discharged into nearby ocean. The most effective and feasible treatment method researched for the effective treatment of foam wastewater generated at the power plants. The result of the test is confirmed with collecting Foam wastewater by inhalation force. The treatment pilot ($3m^3/hr$) collected wastewater was operated by Biological degradation method(Aerobic/anaerobic Processes) for approximately two months. It was removed SS, COD, nutrient(T-P, T-N), etc. The System is expected successfully by Minimizing the operating costs such as electricity, repair expenses, chemicals and supplies expenses.

Effects of Passtein® Supplements on Protein Degradability, Ruminal Fermentation and Nutrient Digestibility (패스틴®첨가가 단백질 분해율과 반추위 발효 및 영양소 소화율에 미치는 영향)

  • Choi, Y.J.;Choi, N.J.;Park, S.H.;Song, J.Y.;Um, J.S.;Ko, J.Y.;Ha, J.K.
    • Journal of Animal Science and Technology
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    • v.44 no.5
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    • pp.549-560
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    • 2002
  • This study, including two in vitro experiments and an in vivo experiment were conducted to evaluate effects of Passtein$^{(R)}$ on crude protein degradability, ruminal fermentation characteristics and nutrient digestibility. In in vitro experiment protein degradability was examined using borate-phosphate buffer and neutral detergent, and using protease from Stroptomyces griseus at 39$^{\circ}C$ for 0, 2, 4, 8, 12, and 48 h. In addition, an in vivo experiment was conducted in a switch back design and ruminal fermentation and nutrient digestibility were determined. Four ruminal-fistulated Holstein cows weighing 300kg in mean body weight randomly allotted to 2 treatments (control and Passtein$^{(R)}$ supplementation). Although there was no significant difference on protein fraction between treatments, it appears that Passtein$^{(R)}$ supplementation decreased buffer soluble protein fraction compared to control. Protein degradability was not affected by Passtein$^{(R)}$ from 0 h to 4 h, but decreased at 12 h and 48 h compared to control. Degradation of immediately degradable fraction was higher in Passtein$^{(R)}$ treatment, but degradation of fermentable fraction was lower in Passtein$^{(R)}$ treatment compared to control. The pH and $NH_3$-N concentration tended to increase in Passtein$^{(R)}$ treatment, but VFA production, microbial counts and enzyme activity tended to decrease in Passtein$^{(R)}$ treatment compared to control. In addition, nutrient digestibility in the total tract tended to increase in Passtein$^{(R)}$ treatment compared to control.

The Concentration and Input/Output of Nitrogen and Phosphorus in Paddy Fields (논에서의 질소 및 인의 농도와 유출입)

  • Shin, Dong-Seok;Kwun, Soon-Kuk
    • Korean Journal of Environmental Agriculture
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    • v.9 no.2
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    • pp.133-141
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    • 1990
  • For the purpose of evaluating nutrient loadings into rivers and lakes from agricultural land, especially from paddy fields and also nutrient degradation in drainage channels, the Total Kjeldahl Nitrogen(TKN) and the Total Phosphorus(TP) were investigated in 29.5 ha. paddy fields in Hwa-Sung, Kyong-Ki, Korea, during the period from May 8, 1989 to Sep. 27, 1989. The results of the study can be su㎜arized as follows : 1. Annual inputs into paddy fields were 180 N-kg/ha 46 P-kg/ha. by fertilization, and 15.0 TKN-kg/ha. 10.0 TP-kg/ha. by irrigation, 8.0 TKN-kg/ha. 0.34 TP-kg/ha. by rainfall respectively. The amount of nutrient involved in surface runoff from paddies was 39.0 TKN-kg/ha. 9.2 TP-kg/ha. and in seepage 7.5 TKN-kg/ha. 2.1 TP-kg/ha. respectively 2. In WS1 stream(reach length equals 950m), nutrients decreased 0.31 TKN-mg/L/km, 0.01 TP-mg/L/km and in WS2 stream (reach length equals 750m) which are more meandering and undulating than WS1, the nutrients decreased 0.84 TKN-mg/L/km, 0.11 TP-mg/L/km. From these results, it was concluded that low stream velocity due to meandering and undulation promotes more degradation of nutrient concentrations. 3. For the purpose of decreasing nutrient loads from paddy fields, the amount of fertilizer used needs to be controlled, irrigation weirs need to be constructed in the drainage channels to delay the transportation of nutrients by decelerating the stream velocity and plants such as plantain-lily need to be cultivated in the channel to consume nutrients and therefore enlarge chances of self-purification.

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A Study on Synthesis of Lignin Peroxidase and Degradation of Pentachlorophenol(PCP) by Phanerochaete chrysosporium (Phanerochaete chrysosporium에 의한 Lignin Peroxidase의 생성과 Pentachlorophenol(PCP)의 분해)

  • Choi, Sue-Hyung;Song, Eun;Gu, Man-Bock;Moon, Seung-Hyeon
    • KSBB Journal
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    • v.13 no.3
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    • pp.223-230
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    • 1998
  • Experiments for lignin peroxidase production have been conducted by aerobic fermentation of Phanerochaete chrysosporium under low shear rate and enriched oxygen environment. The result of flask cultures of white rot fungus indicated that high oxygen concentration and low shear force were essential for enhancement of lignin peroxidase production. Pentachlorophenol was readily degraded by lignin peroxidase produced in nutrient limited flask cultures. Polyurethane foam was fond to be an effective immobilization matrix of P. chrysosporium.

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