• Title/Summary/Keyword: Number of layers

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Revisiting the Parvilucifera infectans / P. sinerae (Alveolata, Perkinsozoa) species complex, two parasitoids of dinoflagellates

  • Jeon, Boo Seong;Nam, Seung Won;Kim, Sunju;Park, Myung Gil
    • ALGAE
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    • v.33 no.1
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    • pp.1-19
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    • 2018
  • Members of the family Parviluciferaceae (Alveolata, Perkinsozoa) are the well-known dinoflagellate parasitoids along with Amoebophrya ceratii species complex and parasitic chytrid Dinomyces arenysensis and contain six species across three genera (i.e., Parvilucifera infectans, P. sinerae, P. rostrata, and P. corolla, Dinovorax pyriformis, and Snorkelia prorocentri) so far. Among Parvilucifera species, the two species, P. infectans and P. sinerae, are very similar or almost identical each other morphologically and genetically, thereby make it difficult to distinguish between the two. The only main difference between the two species known so far is the number of sporangium wall (i.e., 2 layers in P. infectans vs. 3 layers in P. sinerae). During sampling in Masan bay, Korea during the spring season of 2015, the dinoflagellate Akashiwo sanguinea cells infected by the parasite Parvilucifera were observed and this host-parasite system was established in culture. Using this culture, its morphological and ultrastructural features with special emphasis on the variation in the number of sporangium wall over developmental times, were investigated. In addition, the sequences of rDNA regions and ${\beta}-tubulin$ genes were determined. The result clearly demonstrated that the trophocyte at 36 h was covered with 4 layers, and then outer layer of the sporocyte gradually degraded over time, resulting in wall structure consisting of two layers, with even processes being detached from 7-day-old sporangium with smooth surface, indicating that the difference in the number of layers seems not to be an appropriate ultrastructural character for distinguishing P. infectans and P. sinerae. While pairwise comparison of the large subunit rDNA sequences showed 100% identity among P. infectans / P. sinerae species complex, genetic differences were found in the small subunit (SSU) rDNA sequences but the differences were relatively small (11-13 nucleotides) compared with those (190-272 nucleotides) found among the rest of Parvilucifera species (P. rostrata and P. corolla). Those small differences in SSU rDNA sequences of P. infectans / P. sinerae species complex may reflect the variations within inter- strains of the same species from different geographical areas. Taken together, all morphological, ultrastructural, and molecular data from the present study suggest that they are the same species.

Mono- and Multi-layer Langmuir-Blodgett Films of Maleimide Polymers Possessing Nonlinear Optical-Active Side Chains

  • Yoon Kuk Ro;Lee Hoosung;Rhee Bum Ku;Jung Changsoo
    • Macromolecular Research
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    • v.12 no.6
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    • pp.581-585
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    • 2004
  • A copolymer P[OSA-MI] was synthesized by copolymerization of its corresponding monomers, N-phenyl maleimide (MI) and 2-octen-l-ylsuccinic anhydride (OSA). The polymer (poly[2-[1-(2,5-dioxo-l-phenylpyrroli­din-3-ylmethyl)heptyl]-succinic acid 4-(2-$\{$ethyl-[4-(4-nitrophen-ylazo)phenyl]amino$\}$ethyl)ester]) P[DR1MA-MI] was obtained from the reaction of P[OSA-MI] with 2-[4-(4-nitrophenylazo)-N-ethylphenylamino] ethanol (DR1). A stable monolayer of P[DRIMA-MI] was formed by spreading the solution of the polymer in chloroform. In Y-type Langmuir-Blodgett (LB) films prepared using this Langmuir-Blodgett method, the second harmonic waves generated from adjacent mono layers canceled each other out. In X-and Z-type LB films, the second harmonic intensity increased upon increasing the number of monolayers, but this increase was somewhat smaller than predicted by the square law. This phenomenon is due to defects or imperfect alignment of the dipoles in the LB film. The generation of second harmonic waves from Y-type LB films having an even number of mono layers supports this argument. The degree of imperfection seemed to increase as the number of layers increased. The second-order nonlinear optical properties of spin-cast films of these polymers were also measured. The largest second harmonic coefficient of the poled P[DRIMA-MI] film coated on a glass plate was 19 pm/V.

Evaluation of Brinell Hardness of Coated Surface Using Finite Element Analysis: Part 3 - Application to Multilayer Coatings (유한요소해석에 의한 코팅면의 브리넬 경도 평가: 제3보 - 다층 코팅에 적용)

  • Park, TaeJo;Kang, JeongGuk
    • Tribology and Lubricants
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    • v.37 no.6
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    • pp.240-245
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    • 2021
  • Ceramic coatings with high hardness and excellent chemical stability have been successfully applied to various machine elements, tools, and implants. However, in the case of monolayer coating on soft substrates, a high-stress concentration at the interface between the coating and the substrate causes delamination of the coating layer. Recently, to overcome this problem, multilayer coatings with a metal layer with a low modulus of elasticity added between the ceramic and the substrate have been widely applied. This study presents the third part of a recent study and focuses on the effect of the number of coating layers on the Brinell hardness of multilayered coating with TiN/Ti, following the two previous studies on a new Brinell hardness test method for a coated surface and on the influence of substrate and coating thickness. Indentation analyses are performed using finite element analysis software, von Mises stress and equivalent plastic strain distributions, load-displacement curves, and residual indentation shapes are presented. The number of TiN/Ti layers considerably affect the stress distributions and indentation shapes. Moreover, the greater the number of TiN/Ti layers, the higher is the Brinell hardness. The stress and plastic strain distributions confirm that the multilayer coatings improve the wear resistance. The results are expected to be used to design and evaluate various coating systems, and additional study is required.

An Experimental Study on the Compressive Strength Characteristics of Reinforced Concrete Columns Strengthened with Fiber Sheets (섬유시트로 보강된 철근콘크리트 기둥의 압축강도 특성에 관한 실험적 연구)

  • Kim, Jeong-Sup;Choi, Jin-Seok;Cho, Cheol-Hee;Go, Song-Kyoon
    • Journal of the Korea Institute of Building Construction
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    • v.3 no.2
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    • pp.119-127
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    • 2003
  • Test specimen test was performed using concrete reinforced with fiber sheet and the test variables were based on the kinds of fiber and the number of reinforcement layers. Using steel-concrete reinforced with fiber sheet, compression tests were performed and the test variables were the kinds of fiber, number reinforcement layers and reinforcement layer order. The following results were obtained: 1) It was demonstrated that compressive strength of the test specimen reinforced during test specimen test and member test increased as the number of reinforcement layers increased. 2) It was shown that non-reinforced test, specimen were destroyed during the member tests, but the specimen reinforced with CFS destroyed and the GFS-reinforced specimen and composite reinforced specimen showed ductile destruction. 3) As a result of tests on kinds of reinforcement fiber, it was demonstrated that CFS-reinforced test specimen had higher compressive strength in a 공시체 test. In the member test, 2ply-and 3ply-GFS reinforced specimens except lplied one had higher compressive strength. It was because partial destruction occurred due to the rate of height/section. 4) For layer strength order, compared with test specimen reinforced only with a single reinforced material, test specimen reinforced with CFS and GFS, and test specimen reinforced with CFS first showed better results in compressive strength and ductility judgement.

High prandtl number natural convection in a low-aspect ratio rectangular enclosure (종횡비 가 낮은 직각밀폐용기내 의 Prandtl 수 가 큰 유체 의 자연대류 에 관한 실험적 연구)

  • 이진호;황규석;현명택
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.9 no.6
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    • pp.750-756
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    • 1985
  • Experimental investigation was carried out to study the natural convection of water and silicon oil due to end temperature differences in a horizontally insulated rectangular enclosure of aspect ratio 0.1 with a special attention on the core configuration in the laminar boundary-layer flow regime. Rayleigh number ranges covered herein are Ra=4.40 * 10$^{6}$ -9.64 * 10$^{7}$ for water and Ra=1.69*10$^{5}$ -3.80*10$^{6}$ for silicon oil, respectively. In the case of water, for Ra.geq.2.21 * 10$^{7}$ there appeared distinct horizontal thermal layers adjacent to the horizontal boundaries in the core and the temperature distribution outside the horizontal thermal layers, i.e., in the mid-core region, is vertically stratified. The core flow pattern was shown to be nonparallel with a weak back flow in the mid-core for Ra.geq.3.63 *10$^{7}$ . In the case of silicon oil, distinct horizontal thermal layers appeared along the core horizontal boundaries for Ra.geq.1.27 * 10$^{6}$ with a stratified temperature distribution in the mid-core, but the core flow pattern in this case was shown to be parallel. In addition, secondary flow appeared near the hot wall for Ra.geq.3.80 * 10$^{6}$ . Nusselt number, Nu, was found to be proportional to R $a^{0.3}$ for water and R $a^{0.28}$ for silicon oil in the boundary-layer flow regime. There also in an indication from the comparison with other results that Nu is independent of aspect ratio for water in the boundary-layer flow regime in low aspect ratio enclosures.res.

Actual fatigue reliability of structural material: Vibration efficiency

  • Hussain, Muzamal;Khadimallah, Mohamed A.;Ayed, Hamdi;Alshoaibi, Adil;Loukil, Hassen;Alsoruji, Ghazi;Tounsi, Abdelouahed
    • Advances in concrete construction
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    • v.13 no.4
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    • pp.327-337
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    • 2022
  • This paper is concerned with the vibration analysis of middle layer cylindrical shell made of functionally graded material. The outer layers and inner layer are composed of functionally graded and isotropic material respectively. The Rayleigh Ritz method is applied to solve the presented shell dynamics equations. Two configurations are constructed with layers distributions. Fundamental natural frequencies of the three layered cylindrical shell is plotted against the circumferential wave number with different power law exponents. The frequency decreases with the increase of power law exponent. The fundamental natural frequencies first decreases and fall down to its minimum value, after frequencies increases with circumferential wave number. This is due to change in the magnitude of extensional and bending energies of the cylindrical shells. The computer software MATLAB has been employed for the computation of presented frequencies and tested the results obtained in order to assess the accuracy and validity of the cylindrical shell model for predicting the vibration frequencies of cylindrical shell.

Pharmacognostical Studies on the Folk Medicine 'JoRitDae' (민간약 조릿대의 생약학적 연구)

  • Ahn, Mi-Jeong;Bae, Ji-Young;Park, Jong-Hee
    • Korean Journal of Pharmacognosy
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    • v.40 no.4
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    • pp.280-285
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    • 2009
  • Korean folk medicine 'JoRitDae' has been used mainly to cure vomiting and cancer. With regard to the botanical origin of 'JoRitDae', it has been considered to designate the rhizome of Sasa species (Gramineae), but there was no pharmacognostical confirmation on it. To clarify the botanical origin of 'JoRitDae', the anatomical characteristics of the rhizome of Sasa species growing wild in Korea and Japan, Sasa borealis, S, kurilensis, S. quelpaertensis, S. charatacea and S. senanensis were studied. As a result, it was found the morphological criteria for the five Sasa species that could discriminate them by the number of cell layers in cortex, the ratio of thickness of cortex to rhizome radius, the number of cell layers in the vascular bundle sheath, the total number of vascular bundle and thickness of vascular bundle sheath. According to these criteria, it was clarified that the commercial folk medicine 'JoRitDae' was the rhizome of Sasa borealis.

The Strength Properties of Jumchi-Hanji Made from Thai Mulberry Paper (태국산 닥지로 제작한 줌치한지의 강도 특성)

  • Hong, Heesook;Jo, Hyun Jin;Kim, Seong Ju
    • Journal of the Korean Society of Clothing and Textiles
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    • v.46 no.4
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    • pp.565-582
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    • 2022
  • In recent years, Jumchi-Hanji made of mulberry paper is used as a material for fashion items. This study examined the effect of the type of Choji method (Oebal-teugi or Ssangbal-teugi), layer number (one or two layers) and Jumchichigi time (20, 40, or 60 minutes) on the tensile, wet tensile, tearing and bursting strengths of Jumchi-Hanji. Jumchi-Hanjis used as test samples in this study were made from mulbery paper which was manufactured with either bleached or unbleached paper mulberry from Thailand. The results showed that Jumchi-Hanji made with two layers of mulberry papers had higher strength properties than Jumchi-Hanji made with only one layer. The strength properties of Jumchi-Hanji also increased with longer Jumchichigi time. Furthermore, this study confirmed that the effects of Jumchichigi time on Jumchi-Hanji strengths differed based on the type of Choji method, the layer number of mulberry papers used, and the specific strength property measured. Therefore, to guarantee the strength properties required by the application of Jumchi-Hanji paper as a fashion material, it is necessary to carefully consider the adequate Choji method and layer number to apply in the manufacture of Thai mulberry paper and the adequate duration time needed to perform Jumchichigi to Thai mulbery paper.

A Study on the Spatial Resolution of Gas Detectors Based on EGS4 Calculations

  • Moon, B.S.;Han, S.H.;Kim, Y.K.;Chung, C.E.
    • Journal of Radiation Protection and Research
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    • v.29 no.1
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    • pp.25-31
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    • 2004
  • Results of EGS4 based calculations to study the spatial resolution of gas detectors are described. The calculations include radial distribution of electrons generated by photons of various energies penetrating into variable thickness of Ar and Xe gas layers. Given a desired spatial resolution, the maximum allowed thickness of gas layer for each energy level is determined. In order to obtain 0.1mm spatial resolution, the maximum thickness for the Ar gas is found to be 2mm for photon energies below 14keV while the optimum energy of photons for Xe gas with the same thickness is about 45keV. The results of calculations performed to compare the number of electrons generated by CsI coated micro-channel plate and the number of electrons generated by the Ar and Xe gas layers are described. The results show that the number of electrons generated by the gases is about 10 times higher than the one generated by CsI coated micro-channel plate. A few sample gray scale images generated by these calculations are included.

Double-Diffusive Convection in a Salt-Stratified Fluid Heated From Below (농도 성층화된 유체의 아랫면 가열에 의한 이중확산대류에 관한 연구)

  • 강신형;김무현;이진호
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.18 no.12
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    • pp.3297-3304
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    • 1994
  • Experimental investigation have been made to study the double-diffusive nature of convection of an initially stratified salt-water solution heated from below in a cylindrical cavity. The objective is to examine the process of mixed-layer formation, the flow phenomena, the heat transfer characteristics, and temperature and concentration distribution according to the changes in the effective Rayleigh number based on the reference height which represents the relation of temperature and concentration gradient. The types of initially formed flow pattern are categorized in three regimes depending on the effective Rayleigh number ; stagnant flow regime, single mixed-layer flow regime and successively formed multiple mixed-layer flow regime. The temperature and concentration profiles are both uniform in each layer due to convective mixing in the layered flow regime, but both linear in stagnant flow regime and single mixed-layer flow regime. At the interface between adjacent layers, the temperature changes smoothly but the concentration changes rapidly. The layers expand by diffusion of concentration through the interface along with its random fluctuation.