• Journal of Animal Reproduction and Biotechnology
• /
• v.34 no.4
• /
• pp.305-310
• /
• 2019

Embryos produced with serum show the alterations in their ultrastructure, impaired compaction, abnormal blastulation, aberrant mRNA expression profiles and large calf syndrome with greater incidences of stillbirths and deaths after birth. The aim of the present study was to describe in vitro embryo production by analyzing embryo production, fetal production and pregnancy rate in free-serum medium. The OPU-IVP data used in this study from 2016. Approximately, sixteen cows (Hanwoo), which belonged to the Institute of Gyeongsang National University, were used. Two experimental group is used in this study. Serum groups were conducted in March to July and free-serum group was conducted in September to December. The recovered cumulus-oocyte complexes were morphologically classified to four grades based on the compaction of cumulus cells layers and homogeneity of the cytoplasm. The number of oocyte was significantly greater in serum groups than that in free-serum groups (29.61 ± 0.63 vs. 15.6 ± 0.62; p < 0.05). Between serum and free-serum groups indicate that average of 1st and 2nd grade oocytes were no difference (2.38 ± 1.67 vs. 2.38 ± 1.48; p > 0.05), but number of 3rd and 4th grade oocytes were greater in serum groups than that in free-serum groups (7.31 ± 7.64 vs. 5.60 ± 6.29; p < 0.05). Embryo cleaved competence was higher in rate in free-serum groups than that in serum groups (62.1% vs. 58.3; p < 0.05). However, blastocyst developmental rate was no difference between serum and free-serum groups (33.1% vs. 43.5%; p < 0.05). 986 recipients were used for embryo transfer. Pregnancy rate was indicated that between serum and free-serum group was no difference (54.6% vs. 56.3%; p < 0.05). In conclusion, we developed the free-serum system for production of in vitro bovine embryos in order to meet the developmental and qualitative requirements for large scale commercial use.

• Korean Journal of Agricultural Science
• /
• v.10 no.1
• /
• pp.97-109
• /
• 1983

In this study, the effects on the soil compaction were investigated through the various testing method by changing the number of layers, the number of blows per layer and drop height. The results obtained in this study were summarized as follows. 1. Soil compaction was largely influenced by maximum grain size of soil in case of small testing mold diameter. 2. The compaction effect showed best in the well graded soil. In this test, compaction effect showed the best value in the soil which contained about 30~40% of particles finer than No. 200 sieves although it would vary according to the content of coarse grained soil. 3. Though the compaction method was changed at the level of compaction energy fixed, the effect of soil compaction showed little. 4. The increment of compaction energy increased the effect of soil compaction, but over a certain limit the soil compaction showed little on the effect. 5. In the method to increase the compaction energy for the purpose of the most reasonable effect, the soil compaction effect was differently shown according to the grain size distribution of the soil.

• Applied Microscopy
• /
• v.39 no.2
• /
• pp.107-115
• /
• 2009

The programed cell death of the cutaneous epithelial tissue during tail regression stages in anuran tadpoles of the blackspotted frog, Rana nigromaculata were visualized by the histochemical and transmission electron microscopic techniques. Metamorphotic changes in the tail regression during the period of the Shumway stage number 31 to 33 are characterized by the disappearance of mucous layer and formation of compound epithelium through cutaneous thickening. Following the TUNEL (terminal deoxynucleotidyl transferase-mediated biotinylated d-uridine triphosphate nick end labeling) staining technique, the apoptotic cells were detected at the distal region of the tail skin initially, but they can be seen at the proximal region according to their following development. It has been also revealed that the number of the TUNEL-positive cells gradually increased from apical to basal direction of the epithelial layers during the tail regressing stages. Following the TEM observation, the early apoptotic cells shown in the epithelium demonstrated condensation and margination of the chromatin material at the nuclear periphery. Another epithelial apoptotic cells were shown nuclear fragmentation, membrane blebbing and cytoplasmic condensation. Following the process of the apoptotic degradation, well preserved organelles and nuclear fragments can be identified in the cytoplasm of lysosome-rich cells, however they soon reduced to lysosomal residual bodies through the progressive degradation.

• 한국지구물리탐사학회:학술대회논문집
• /
• 1999.08a
• /
• pp.48-64
• /
• 1999

For quantitative evaluation of geotechnical engineering properties such as rippability and diggability, clear interpretation on the subsurface velocity structures should be preceded by figuring out top soil, weathered and soft rock layers, shape of basement, fracture zones, geologic boundary and etc. from the seismic refraction data. It is very important to set up suitable field parameters, which are the configuration of profile and its length, spacings of geophones and sources and topographic conditions, for increasing field data quality Geophone spacing of 3 to 5m is recommended in the land slope area for house land development and 5 to 10m in the tunnel site. In refraction tomography technique, the number of source points should be more than a half of available channel number of instrument, which can make topographic effect ignorable. Compared with core logging data, it is shown that the velocity range of the soil is less than 700m/s, weathered rock 700${\~}$1,200m/s, soft rock 1,200${\~}$1,800m/s. And the upper limit of P-wave velocity for rippability is estimated 1,200 to 1,800m/s in land slope area of gneiss. In case of tunnel site, it is recommended in tunnel design and construction to consider that tunnel is in contact with soft rock layer where three lineaments intersecting each other are recognized from the results of the other survey.

• The Journal of Engineering Geology
• /
• v.11 no.2
• /
• pp.163-174
• /
• 2001

For quantitative evaluation of geotechnical engineering properties such as rippability and diggability, clear interpretation on the subsUJiace velocity structures should be preceded by figuring out top soil, weathered and soft rock layers, shape of basement, fracture zones, geologic boundary and etC. from the seismic refraction data. It is very important to set up suitable field parameters, which are the configuration of profile and its length, spacings of geophones and sources and topographic conditions, for increasing field data Quality. Geophone spacing of 3 to 5m is reconunended in the land slope area of house land development site. In refraction tomography technique, the number of source points should be more than a Cluarter of available channel number of instrument and the subsurface structure interpretation can be decreased the artifact of inversion by topographic effect. Compared with core logging data, it is shown that the velocity range of the soil is less than 700m/s, weathered rock 700~1,200m/s, soft rock 1,200~1,800m/s on the velocity tomogram section. And the upper limit of P-wave velocity for rippability is estimated 1,200 to 1,800m/s in land slope area of gneiss.

• Korean Journal of Environment and Ecology
• /
• v.24 no.2
• /
• pp.166-177
• /
• 2010

This study is to identify potential factors to impact on appearance of wildbirds which live in the core forest around urban area in Gangdong-gu, Seoul. As a result of analysis of, studies on biotope showed most of urbanization biotope was biotope of residential areas with high green coverage and biotope of residential and business areas with low green coverage while most of biotope of green and openspace was core green biotope. The research area was divided into several blocks based on biotope types in the urbanization areas excluding green and openspace. As a result of research on wildbirds, total 51 species 3,419 individuals appeared in spring and total 35 species 4,213 individuals appeared in winter. 24 wild bird species were selected as subjects of this study among 31 species seen in urbanization areas, since urban birds, rapacious birds, waterside birds were excluded from the study for the proper consideration. Then this study looked at how many species and individuals of the subjects were observed at each research block in urbanization areas during spring and winter separately. Landuse structure and green structure in each block were examined to see whether these structures affect the number of wild birds observed in urbanization areas of Gangdong-gu. Furthermore, the distance between these blocks and green was assessed. While studying the potential links between the landuse structure and the number of wild birds observed in urbanization areas of Gangdong-gu, block area, green coverage, and building-to-land ratio were believed to affect the number of types and species of wild birds in the research area. In terms of correlation analysis of whether green structure affected the number of wild birds observed in urbanization areas of Gangdong-gu, crown volume of layers, the average green patch area, the average height of canopy layer were found to have an impact not only on the number of types but also species of wild birds in the research area.

• Journal of Wetlands Research
• /
• v.8 no.3
• /
• pp.79-89
• /
• 2006

The survival rate and tube number of Monocorophium uenoi, one of the dominant species in Lake Sihwa, was investigated under laboratory condition with salinity gradients (1, 5, 10, 15, 20, 25, 30, 35, 40‰). Another laboratory experiments were conducted to investigate the survival rate of Monocorophium uenoi in sulphide-rich sediment with oxic overlying water, and the effect of amphipod bioturbation on sulphide and oxygen profiles. The survival rate and tube-forming number of the amphipod were high in salinity range of 20 to 30‰. The amphipod survival rate was also high in sulphidic sediment when the water column was oxic. The amphipod bioturbation affected the oxygen and sulphide content in the sediment; oxygen conditions in the upper sediment layers were improved, and simultaneously the concentrations of sulphide were reduced. And their depth was clearly dependent on amphipod density. In Lake Sihwa, previously anoxic bottoms with sulphidic sediment during summer turn oxic with pycnocline disappearance during autumn. Amphipods seem to have the capability to quickly invade such areas, and their tubing of the sediment may play an important role in preparing the sediment for further recolonisation of other macrobenthos.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.12
• /
• pp.106-111
• /
• 2017

In this study, the stiffness of an LMU (Leg Mating Unit), which is a device required for installing the top side part of an offshore structure, was examined through structural analysis. This unit is mounted on the supporting point of the structure and is used to absorb the shock at installation. It is a cylindrical structure with an empty center. To support the vertical load, elastomeric bearings (EBs) and iron plates are laminated in layers. The stiffness of the EBs is basically influenced by the size of the bearings, but it varies with the number of laminated sheets inside the same sized structure. The relationship between the stiffener and the compressive stiffness is investigated, and its design is suggested. The stiffness of the EBs is analyzed by calculating the reaction force, while controlling the displacement. First, the relationship between the size of the reinforcing plate and the compressive stiffness is considered. Next, the relationship between the number of stacked reinforcing plates and the compression stiffness is considered. Different loads are required for each installed point. The goal is to design the compression stiffness in such a way that the same deformation occurs at each point in the analysis. In this study, ANSYS is used to perform the FE analysis.

• Journal of Convergence for Information Technology
• /
• v.11 no.5
• /
• pp.30-37
• /
• 2021

Various devices are connected to the Internet, and attacks using the Internet are occurring. Among such attacks, there are attacks that use malicious URLs to make users access to wrong phishing sites or distribute malicious viruses. Therefore, how to detect such malicious URL attacks is one of the important security issues. Among recent deep learning technologies, neural networks are showing good performance in image recognition, speech recognition, and pattern recognition. This neural network can be applied to research that analyzes and detects patterns of malicious URL characteristics. In this paper, performance analysis according to various parameters was performed on a method of detecting malicious URLs using neural networks. In this paper, malicious URL detection performance was analyzed while changing the activation function, learning rate, and neural network structure. The experimental data was crawled by Alexa top 1 million and Whois to build the data, and the machine learning library used TensorFlow. As a result of the experiment, when the number of layers is 4, the learning rate is 0.005, and the number of nodes in each layer is 100, the accuracy of 97.8% and the f1 score of 92.94% are obtained.

• Journal of Korean Society for Geospatial Information Science
• /
• v.22 no.1
• /
• pp.89-99
• /
• 2014

The current Development Impact Fee (DIF) Zoning Law allows Korean localities to designate the DIF Zone for the areas where there have been up-zoning in land-uses due to any minute additions and/or amendments in the existing clauses or provisions in the National Territory Planning Law as well as all other laws related to urban and regional planning. In reality, however, it is almost impossible to trace the sporadic and infinitesimal changes that might occur in every corner of the statutory clauses of a great number of planning related laws. This study, in an effort to overcome such practical difficulties, tries to chase the time-series zoning alterations in especially the consecutive land-use information layers of the Korea Land Information System (KLIS) as comparable analogues of the outcomes of the amendments in various planning laws. A study locality is chosen among the entire localities in the Capital Region based on the selection criteria dictated by the DIF Zoning Law such as the population- and building permit increase rate. It has been verified that the methodology suggested herein is practically applicable and successfully capable of extracting a number of DIF zones with considerable areal sizes, which could not have otherwise been possible. The consequences of this study, in this context, are expected to contribute to prevent the uncontrolled developments as the DIF Zoning Law itself was originally intended to achieve.