• Molecules and Cells
• /
• 제45권7호
• /
• pp.465-478
• /
• 2022

MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target messenger RNA (mRNA) complementary to the 3' untranslated region (UTR) at the post-transcriptional level. Hsa-miR-422a, which is commonly known as miRNA derived from transposable element (MDTE), was derived from short interspersed nuclear element (SINE). Through expression analysis, hsa-miR-422a was found to be highly expressed in both the small intestine and liver of crab-eating monkey. AT-Rich Interaction Domain 5 B (ARID5B) was selected as the target gene of hsa-miR-422a, which has two binding sites in both the exon and 3'UTR of ARID5B. To identify the interaction between hsa-miR-422a and ARID5B, a dual luciferase assay was conducted in HepG2 cell line. The luciferase activity of cells treated with the hsa-miR-422a mimic was upregulated and inversely downregulated when both the hsa-miR-422a mimic and inhibitor were administered. Nuclear factor erythroid-2 (NF-E2) was selected as the core transcription factor (TF) via feed forward loop analysis. The luciferase expression was downregulated when both the hsa-miR-422a mimic and siRNA of NF-E2 were treated, compared to the treatment of the hsa-miR-422a mimic alone. The present study suggests that hsa-miR-422a derived from SINE could bind to the exon region as well as the 3'UTR of ARID5B. Additionally, hsa-miR-422a was found to share binding sites in ARID5B with several TFs, including NF-E2. The hsa-miR-422a might thus interact with TF to regulate the expression of ARID5B, as demonstrated experimentally. Altogether, hsa-miR-422a acts as a super enhancer miRNA of ARID5B by collaborating with TF and NF-E2.

• 스마트미디어저널
• /
• 제11권10호
• /
• pp.46-53
• /
• 2022

오늘날 4차 산업 혁명과 대규모 R&D 지원으로 인해 국내 기업은 세계 기술력 수준의 산업기술을 보유하기 시작하였으며 중요한 자산으로 변모하였다. 국가는 기업의 중요한 산업기술을 보호하고자 국가핵심기술로 지정하였으며, 특히 원자력, 조선, 반도체와 같은 기술이 유출될 경우 해당 기업뿐만 아니라 국가 차원에서도 심각한 경쟁력 손실로 이어질 수 있다. 매년 내부자 유출, 랜섬웨어 그룹의 해킹공격, 산업스파이에 산업기술 탈취 시도가 증가하고 있으며, 탈취된 산업기술은 다크웹 환경에서의 은밀하게 거래가 이루어진다. 본 논문에서는 다크웹 환경에서 은밀하게 이루어지는 산업기술 유출을 탐지하는 시스템을 제안한다. 제안된 모델은 먼저 OSINT 환경에서 수집한 정보를 이용하여 다크웹 크롤링을 통한 데이터베이스를 구축한다. 이후 KeyBERT 모델을 이용한 산업기술 유출 키워드를 추출한 후 다크웹 환경에서의 산업기술 유출 징후를 정량적 수치로 제안한다. 마지막으로 식별된 다크웹 환경에서의 산업기술 유출 사이트를 기반으로 PageRank 알고리즘 통한 2차 유출 가능성을 탐지한다. 제안된 모델을 통해 27,317개의 중복 없는 다크웹 사이트를 수집하였으며, 100개의 원자력 특허에서 총 15,028개의 원자력 관련 키워드를 추출하였다. 가장 높은 원자력 유출 다크웹 사이트를 기반으로 2차 유출을 탐지한 결과 12개의 다크웹 사이트를 식별하였다.

• 대한토목학회논문집
• /
• 제43권6호
• /
• pp.699-708
• /
• 2023

원자력 발전소는 안전하게 운영될 수 있도록 설계단계에서 자연재해를 포함한 예상 가능한 모든 외부사건들이 고려되고 있다. 그러나 최근에 기후변화로 인해 설계를 초과하는 자연재해의 발생 가능성이 증가하면서 설계 당시에 예측하지 못했던 극한자연재해와 외부사건에 대해 세심한 검토가 요구되고 있다. 본 연구에서는 국내 원전 부지별 잠재적 극한자연재해를 선별하고자 하였다. 이를 위해서 원전 부지특성과 원전 부지별 외부재해로 인해 발생한 사건을 조사하고 분석하였다. 그리고 기존 문헌과 연구 자료를 조사하여 국내 원전 실정에 맞는 선별절차와 기준을 수립하였다. 본 연구에서 수립된 선별기준에 근거하여 원전 부지별로 정성적 선별을 수행하였고, 선별된 자연재해를 대상으로 정량적 선별과 현장실사를 통해 국내 원전 부지별 잠재적 극한자연재해를 선별하였다. 선별 결과, 폭우에 의한 내부침수 이외에도 강풍에 의한 풍압과 극한 공기압이 모든 부지에서 공통적인 잠재적 극한자연재해로 선별되었다. 그리고 고리부지의 경우 폭풍해일이 1순위의 잠재적 극한자연재해로 선정되었다.

• 방사성폐기물학회지
• /
• 제5권4호
• /
• pp.351-357
• /
• 2007

일본 정부는 사용후핵연료의 중간저장시설의 입지로 아우모리현의 무쯔시를 선정했다. 2000년부터 시작된 유치활동이 5 년만에 결실을 맺은 것이다. 한국은 사용후핵연료를 원자력 발전소 내에 저장하고 있는데 2016년이면 저장한계에 이를 것으로 전망되고 있어 일본의 사례연구는 한국에 시사하는 바가 크다. 한국은 경주에 중, 저준위 방사성 폐기물 저장시설을 성공적으로 유치한 경험이 있어 사용후핵연료의 저장시설을 유치하는 학습경험을 축적한 바 있는데 두 나라의 큰 차이점은 한국은 경쟁적인 주민투표를 통하면서 막대한 지역지원금을 지원한다는 점이고 일본은 주민투표를 시행하지 않고 지방자치 정치의 리더쉽이 문제를 해결하는 방식을 택했다는 점이다. 엄청난 지역지원금이 지원되지 않은 점도 일본의 특징이다. 본 연구가 한국의 사용후핵 연료 저장시설을 유치하는 데 도움이 되었으면 하는 바람이다.

• 대한핵의학회지
• /
• 제33권6호
• /
• pp.537-542
• /
• 1999

In patient with Zollinger-Ellison syndrome, it is difficult to localize gastrinoma because the tumor is frequently small and multiple. However, accurate localization of the tumor is important for the treatment Among various imaging modalities, somatostatin receptor scintigraphy (SRS) has been recognized to be the most sensitive tool for the detection of neuroendocrine tumors such as gastrinomas based on the presence of high-affinity binding sites for somatostatin. Recently, we experienced a case of Zollinger-Ellison syndrome caused by gastrinomas which was localized by SRS. This is the first case report of gastrinoma detected by SRS in Korea. SRS can facilitate tumor detection in patient with Zollinger-Ellison syndrome and should be considered as the first-line diagnostic method in the early course of the disease.

• ALGAE
• /
• 제20권3호
• /
• pp.217-224
• /
• 2005

The species composition and biomass of marine algae at the discharge canals of three (Gori, Wolseong and Uljin) nuclear power plants on the east coast of Korea were investigated seasonally from February 1992 to October 2000. As a result, 103-107 species of marine algae were found at the discharge canals during the past nine years. In general, algal communities established at the discharge canals were less diverse than those at the intake canals and control sites. 43 species (6 blue-green, 9 green, 10 brown and 18 red algae) of marine algae occurred more than 1/6 frequency and thus can be categorized as warm tolerant species. Among these, two green (Urospora penicilliformis, Cladophora albida), four brown (Sphacelaria divaricata, S. rigidula, Sargassum coreanum, S. fulvellum) and four red algae (Stylonema alsidii, Bangia atropurpurea, Hypnea charoides, Chondria crassicaulis) are recorded as warm tolerant marine algae for the first time in Korea during this study. Enteromorpha compressa, Padina arborescens, Amphiroa zonata and Ahnfeltiopsis flabelliformis were common species found more than 50% frequency at the discharge canals of all three nuclear power plants investigated. Dominant species in biomass were Padina arborescens and Amphiroa zonata. Results showed that, as a whole, the red algae appeared as predominant algal group at the discharge canals of all three nuclear power plants on the east coast of Korea. However, the biomass proportion of dominant algae at the discharge canals of each nuclear power plant varied over the year during the past nine years.

• Nuclear Engineering and Technology
• /
• 제53권1호
• /
• pp.234-243
• /
• 2021

In the research or project planning for the decommissioning of a nuclear power plant, one of several preparations will be the establishment of a list of potential radionuclides to be considered at the time of characterization or final status surveys. Reliable data for selection of potential radionuclides during the transition period to prepare for decommissioning will depend heavily on historical data at the site or, where possible, sampling analysis. However, during the transition period, direct sampling can be challenging, depending on the circumstances of the site or national regulation. A methodology of selecting potential radionuclides for nuclear facility sites which largely consists of three major processes: production of initial list of radionuclides, selection of the insignificant radionuclide that will be eliminated, and consideration of site characterization or sampling. For developing a preliminary list of potential radionuclides for Kori Unit 1 decommissioning, the list of initial radionuclides was made referring to the technical documents applied at decommissioned NPPs in the U.S and additional reference materials applied until the operation of NPPs in Korea. For the screening of insignificant radionuclides, we applied criterion of less than 0.1% of the amount of radioactivity inventory and confirmed the dose fraction using the RESRAD code. The final suit of radionuclides was established, which should be supplemented by reflecting site characterization and sampling process in the future. Thus, the methodology and results for the selection of potential radionuclides suggested in this paper can give an insight as a future reference to deriving DCGLs in relation to site remediation of decommissioning nuclear plants.

• BMB Reports
• /
• 제43권6호
• /
• pp.407-412
• /
• 2010

Homeodomain (HD) is a highly conserved DNA-binding domain composed of helix-turn-helix motif. Drosophila Vnd (Ventral nervous system defective) containing HD acts as a regulator to either enhance or suppress gene expression upon binding to its target sequence. In this study, kinetic analysis of Vnd binding to DNA was performed. The result demonstrates that DNA-binding affinity of the recombinant protein containing HD and NK2-specific domain (NK2-SD) was higher than that of the full-length Vnd. To access whether phosphorylation sites within HD and NK2-SD affect the interaction of the protein with the target sequence, alanine substitutions were introduced. The result shows that S631A mutation within NK2-SD does not contribute significantly to the DNA-binding affinity. However, S571A and T600A mutations within HD showed lower affinity for DNA binding. In addition, DNA-binding analysis using embryonic nuclear protein also demonstrates that Vnd interacts with other nuclear proteins, suggesting the existence of Vnd as a complex.

• 한국방사성폐기물학회:학술대회논문집
• /
• 한국방사성폐기물학회 2005년도 Proceedings of The 6th korea-china joint workshop on nuclear waste management
• /
• pp.126-140
• /
• 2005

The tomographic gamma scanner (TGS) method, a further of extension of segmented gamma scanner (SGS), is most accurate and precise for assaying heterogeneous drummed nuclear radioactive waste; it is widely used in nuclear power plants and radioactive waste storages and disposal sites. The transmission and emission images are reconstructed by image reconstruction techniques. In the paper, the principle of TGS is introduced; image reconstruction techniques are discussed as well; finally, it is demonstrated that TGS method performance.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1995년도 추계학술발표회논문집(2)
• /
• pp.893-898
• /
• 1995

The cumulative deposition of Pu-238 and Pu-239,240 by the end of 1994 in undisturbed for the last 40 years, was determined at 9 sites in Korea. The cumulative deposition of Pu-238 and Pu-239 ranged from 0.76 to 3.77 and from 18.42 to 101.84 $Bqm^{-2}$, respectively. The mean values of the cumulative deposition of Pu-238 and Pu-239,240 were 2.16 and 54.75 $Bqm^{-2}$, respectively. These values are close to the value of worldwide fallout. No significant contribution to the cumulative deposition of Pu-238 and Pu-239,240 originating from the Chernobyl accident was found at my site.