• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.123-126
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• 2008

The objective of this study was to determine the potential hazardous effects of sorting process by flowcytometry on the quality of boar spermatozoa by flowcytometer. Freshly collected boar semen was diluted and divided into two groups; control none sorted and sorted. Sperms in sorted group were processed with flowcytometer for cell sorting with $100\;{\mu}M$ nozzle under the 20 psi pressure. Measurements on each parameter were made at two time points, 0hr (right after sorting) and 24 hr post sorting. Although there was a tendency of lower viability in sorted group than none sorted control group, the percentage of live cells in control ($75.83{\pm}6.92\;&\;59.53{\pm}10.34$) was not significantly different from sorted ($59.70{\pm}7.37\;&\;43.97{\pm}3.76$) at both 0 and 24 hr post sorting. However, sorted sperm showed significantly lower mitochondrial function compared to the control at both 0 h ($79.37{\pm}3.22\;vs.\;63.50{\pm}10.05$) and 24 hr ($67.27{\pm}3.22$ vs. $46.97{\pm}5.37$) time points (p<0.007). Sperm DNA fragmentation rate was significantly lower in control ($22.0{\pm}7.04$) than that of sorted ($32.27{\pm}7.49$) at 24 hr time point (p<0.0002). Taken together, these data suggested thatsorting process by flowcytometer may have influenced sperm motility rather than viability. Also high speed sperm sorting by flowcytometer has significant effects on DNA fragmentation on elapsed time after sorting.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.4
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• pp.53-60
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• 2004

Optimal design of the hybrid motor has been performed for the first stage of nanosat air launch vehicle using F-4E Phantom as mother plane. Selected design variables are number of ports, the initial oxidizer flux, the combustion chamber pressure, and the nozzle expansion ratio. GBM(Gradient Based Method) and GA(Genetic Algorithm) are simultaneously used to compare the versatility of each algorithm for optimal design in this problem. Also, two objective functions of motor weight, and length are treated separatedly in the optimization to study how the objective function can affect the optimal design. The design results show that the optimal design can be successfully achieved either using GBM or GA regardless of the choice of the objective function; motor weight or length. And nanosat air launch vehicle which has total mass of 704.74kg, and length of first stage 3.74m is designed.

• Journal of the Korean Society of Propulsion Engineers
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• v.3 no.4
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• pp.58-66
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• 1999

The flow characteristics of supersonic ejectors is often subject to compressibility, unsteadiness and shock wave systems. The numerical works carried out thus far have been of one-dimensional analyses or some Computational Fluid Dynamics(CFD) which has been applied to only a very simplified configuration. For the design of effective ejector-pump systems the effects of secondary mass flow on the supersonic ejector flow should be fully understood. In the present work the supersonic ejector-pump flows with a secondary mass flow were simulated using CFD. A fully implicit finite volume scheme was applied to axisymmetric compressible Navier-Stokes equations. The standard two-equation turbulence model was employed to predict turbulent stresses. The results obtained showed that the flow characteristics of constant area mixing tube types were nearly independent of the secondary flow rate, but the flow fields of ejector system with the second-throat were strongly dependent on the secondary flow rate due to the effect of the back pressure near the primary nozzle exit.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2011.11a
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• pp.981-984
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• 2011

A high-resolution numerical study is carried out to investigate the transient process of the combustion and the shock-train developments in an ethylene-fueled direct-connect dual-mode scramjet combustor. Following the fuel injection, air-throttling is applied at the expansion part of the combustor to provide mass addition to block the flow to subsonic speed. The ignition occurs several ms later when the fuel and air are mixed sufficiently. The pressure build up by the combustion leads to the shock train formation in the isolator section that advances to the exit of the intake nozzle. Then, the air-throttling is deactivated and the exhaust process begins and the situation before the air-throttling is restored. Present simulation shows the detailed processes in the dual-mode scramjet combustor for better understanding of the operation regimes and characteristics.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2011.11a
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• pp.540-543
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• 2011

This work is devoted to an experimental investigation on conceptual design of dual consecutive stage micro plasma thruster (${\mu}PT$). Optimization study on the thruster configuration has been performed for various electrode gap distances from 1 mm to 2 mm and the hole diameter from 0.3 mm to 2 mm depending on desired operating conditions and corresponding nozzle design requirement. The operation of ${\mu}PT$ at low pressure from $10^{-1}$ Torr to $10^{-4}$ Torr and at various argon flow rates ranging from 5 sccm to 300 sccm has been studied to understand the physic of plasma and the gas dynamics in details. The specific impulse can reach up to 3000-4000 seconds at low power consumptions from 1 to 5 W. Image of exhaust plume from ${\mu}PT$ will be provided and electrical characteristics is also mentioned in this paper.

• The Journal of the Acoustical Society of Korea
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• v.15 no.6
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• pp.52-58
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• 1996

Using the acoustic resonances and oscillatory pulsations considered as the branch of wave technologies, the concept of the acoustic resonance diffusers for waste water treatment which maximize the oxygen transfer efficiency in gas-liquid two phase medium have been proposed, and studies for the principles and performance tests were accomplished. Besides, the design concepts for the low pressure Helmholtz resonator, cylinder and annular type reflection resonator and combined type resonance system have been implemented. The acoustic resonance energy which can speed up the mass transfer process increase the oxygen transfer efficiency, and periodic pulsations generated from the instability of air jet from nozzle make very small air bubbles. Then, the annular type jet resonator(AJR) applying these two principles successfully was evalulated as the most promising device and also the efficiency showing $20{\sim}30%$ better than conventional diffusers has been verified experimentally.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.05a
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• pp.133-137
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• 2008

Results of combustion tests performed for a regenerative cooling combustor of a 30 tonf-class liquid rocket engine were described. The combustion chamber has chamber pressure of 60 bar, propellant mass flow rate of 89 kg/s, and nozzle expansion of 12. The combustion chamber is composed of mixing head, baffle injector, and regenerative cooling chamber. The hot firing tests were performed at design and off-design points. The test results show that the combustion characteristic velocity is in the range of 1738${\sim}$1751 m/sec and the specific impulse of the combustion chamber is in the range of 253${\sim}$270 sec. The peak of combustion characteristic velocity and specific impulse for this combustor is shown at mixture ratio of 2.35 and 2.5, respectively.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.11a
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• pp.503-506
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• 2009

In this study, we presented the results of static firing tests on the PE/LN2O hybrid rocket motor, which has a thrust of 650 kgf level. Through the early tests, we found that the combustion chamber pressure and the thrust were lower than design values because an actual oxidizer flow rate was less than that expected. In order to complement this result, the methods of decrease of nozzle throat and the increase of oxidizer mass flow rate were conducted in the next experiment, and we studied the combustion phenomena with the experimental results. Also we compared and analyzed a difference of combustion characteristics on scale effect. It show that a sub-scale motor regression rate was a little less than that of a lab-scale motor with the same oxidizer mass flux. Results of this study might be used as a basic data for development of hybrid sounding rocket.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.11a
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• pp.573-577
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• 2009

Dynamic behavior simulation of supersonic engine was performed and PI control algorithm was studied for the buzz control in the inlet and the thrust control. Firstly, required thrust was tracked according to the fuel flow control and then inlet pressure was regulated through the nozzle throat area control so that the buzz margin has the positive all the time. The control was performed according to the change of flight Mach number, altitude and angle of attack. The proportional gain and the integral gain for regulating the buzz margin was induced and simulated. In the results, it was confirmed and satisfied that control target in the operating area was changed the angle of attack from $0^{\circ}$ to $10^{\circ}$ at the flight Mach number of 2.1~3.0.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2009.05a
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• pp.88-92
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• 2009

Total pressure recovery ratio in intake is crucial factor to the operational characteristics of supersonic propulsion system because it does not compress inlet air mechanically by compressor, but does compress inlet air by ram compression. As the result of that the dynamic characteristic analysis of engine was performed before the controller was designed, it could be ascertained when the AoA of flight vehicle increases, the buzz margin decreases so that the shock wave produced outside intake in the specified area according to flight operation's characteristics. Therefore the PID control algorithm was designed to be controlled buzz margin that the characteristic of shock wave could meet the requirement of performance in intake. The PID controller was designed that the buzz margin value is being positive number using the control variables; fuel flow and nozzle throat area.