• Composites Research
• /
• 제12권1호
• /
• pp.68-75
• /
• 1999

본 연구에서는 저속 충격에서 충격 속도에 따른 금속복합재료의 동적 거동을 연구하였다. 시험에 사용된 재료는 모재로 AC8A와 보강재로 알루미나($Al_2O_3$)와 탄소를 사용하였으며 용탕 주조법을 이용하여 금속복합재료를 제조하였다. 금속복합재료에는 15%의 부피분율을 가진 알루미나 예비성형체와 알루미나와 탄소를 각각 12%와 3% 사용한 혼합 에비성형체가 사용되었다. 제조된 금속복합재료는 인장 시험과 진동 시험을 통해 인장 강도와 탄성계수를 구하였으며, 저주파 여파기(low pass filter)와 계장화 충격 시험기를 이용하여 충격 속도에 따른 금속복합재료의 충격 거동을 연구하였다. 저주파 여파기를 이용함으로써 충격 속도에 관계없이 안정적인 실험치를 확보할 수 있었다. 충격 속도의 증가에 따라 모재와 금속복합재료의 충격에너지는 증가하였으나, 동적인성치는 일정한 값을 보였다. 충격 속도가 증가할수록 충격에너지 중 균열전파에너지의 향상이 두드러졌으며, 재료의 변형량이 증가하였다. 충격에너지 중 균열개시에너지와 동적파괴인성치의 관계를 설명하기 위하여 변형율 에너지와 노치에서의 응력 분포를 이용하여 간단한 모델을 제시하였으며, 이로부터 균열개시에너지는 동적 파괴 인성치의 자승에 비례하고 탄성계수에 반비례하는 것을 보였다.

• 열처리공학회지
• /
• 제23권4호
• /
• pp.183-190
• /
• 2010

There are many methods to reduce the weight and the cost of the automobile body, among them, Tailor Welded Blank (TWB) is new welding method applied to body structure. It is necessary to evaluate mechanical properties of TWB structures or sheets for the application to automobile body parts. In this study, the stiffness of T-type and L-type joint structures, composite of TWB panel, which simplified two portions of side structure in automobile body were investigated. Additionally, the fatigue properties of TWB panels were obtained. Two types of welding technologies, laser and mash seam welding, were used to join mild panels with different thickness. This results are compared with conventional structures. The results are as follows: 1) The stiffness of joint structures, composite of TWB panel, is approximately 17% higher than that of conventional ones. 2) The location of welding line in TWB had a effect on the in plane bending stiffness, but not on the out of plane bending stiffness. 3) In terms of welding technology type, the mash seam welding show higher stiffness than the laser welding for in plane bending stiffness. But minimal differences in both types are revealed for out of plane bending stiffness. 4) The fatigue strength, composite of TWB panel, is lower than that of base steel. It is thought that defects in the welding zone had the action of notch in the fatigue test.

• Restorative Dentistry and Endodontics
• /
• 제23권2호
• /
• pp.690-700
• /
• 1998

Rubber-toughened particles which are used in the field of chemical engineering are used to increase the fracture toughness of thermoset resin. The application of Core-Shell particles, one of rubber-toughened particles, as a filler for dental composite or restoration have not been examined. The purpose of this study was to evaluate possible use of Core-Shell particles for dental composite, and the hypothesis was that additional toughening mechanisms are activated by the addition of Core-Shell particles. After blending 50vol% quartz with Bis-GMA/TEGDMA resin matrix, the experimental resins were made by the addition of Core-Shell particles with varied content level as 0, 2.5, 5, 7.5, 10, 12.5, 15, and 20wt%. Fracture toughness was determined on three-point bending specimen with single-edge notch according to ASTM-E 399. Also, flexural properties, that is, strength and modulus were measured by three-point bending testing. Fractogragh of fracture toughness specimen was observed using SEM (JEOL 6400 SEM, MA). The following results from this study were obtained ; 1. Fracture toughness of composite resin added 2.5wt% Core-Shell particles was significantly higher than control group ($p{\leq}0.05$). 2. Flexural properties were decreased with increasing Core-Shell particle content, which showed a correlation statistically ($p{\leq}0.05$). 3. A toughening mechanism such as lamination and microcrack was observed in specimen determined high fracture toughness. 4. The dispersion of Core-Shell itself and quartz filler particles was limited present high content of Core-Shell particles, which decreased a resulting mechanical properties of composites. These results suggest that adequate Core-Shell particles can be used to enhance mechanical properties included toughening for dental composites.

• Design & Manufacturing
• /
• 제14권3호
• /
• pp.23-30
• /
• 2020

Laser Assisted Thermo-Compression Bonding (LATCB) has been proposed to improve the "chip tilt due to the difference in solder bump height" that occurs during the conventional semiconductor chip bonding process. The bonding module of the LATCB system has used a piezoelectric actuator to control the inclination of the compression jig on a micro scale, and the piezoelectric actuator has been directly coupled to the compression jig to minimize the assembly tolerance of the compression jig. However, this structure generates a lateral force in the piezoelectric actuator when the compression jig is tilted, and the stacked piezoelectric element vulnerable to the lateral force has a risk of failure. In this paper, the optimal design of the flexure hinge was performed to minimize the lateral force generated in the piezoelectric actuator when the compression jig is tilted by using the displacement difference of the piezoelectric actuator in the bonding module for LATCB. The design variables of the flexure hinge were defined as the hinge height, the minimum diameter, and the notch radius. And the effect of the change of each variable on the stress generated in the flexible hinge and the lateral force acting on the piezoelectric actuator was analyzed. Also, optimization was carried out using commercial structural analysis software. As a result, when the displacement difference between the piezoelectric actuators is the maximum (90um), the maximum stress generated in the flexible hinge is 11.5% of the elastic limit of the hinge material, and the lateral force acting on the piezoelectric actuator is less than 1N.

• Molecules and Cells
• /
• 제37권5호
• /
• pp.406-411
• /
• 2014

The initial step of atrioventricular (AV) valve development involves the deposition of extracellular matrix (ECM) components of the endocardial cushion and the endocardialmesenchymal transition. While the appropriately regulated expression of the major ECM components, Versican and Hyaluronan, that form the endocardial cushion is important for heart valve development, the underlying mechanism that regulates ECM gene expression remains unclear. We found that zebrafish crip2 expression is restricted to a subset of cells in the AV canal (AVC) endocardium at 55 hours post-fertilization (hpf). Knockdown of crip2 induced a heart-looping defect in zebrafish embryos, although the development of cardiac chambers appeared to be normal. In the AVC of Crip2-deficient embryos, the expression of both versican a and hyaluronan synthase 2 (has2) was highly upregulated, but the expression of bone morphogenetic protein 4 (bmp4) and T-box 2b (tbx2b) in the myocardium and of notch1b in the endocardium in the AVC did not change. Taken together, these results indicate that crip2 plays an important role in AV valve development by downregulating the expression of ECM components in the endocardial cushion.

• Molecules and Cells
• /
• 제37권4호
• /
• pp.330-336
• /
• 2014

Thymosin beta4 (TB4) has multiple functions in cellular response in processes as diverse as embryonic organ development and the pathogeneses of disease, especially those associated with cardiac coronary vessels. However, the specific roles played by TB4 during heart valve development in vertebrates are largely unknown. Here, we identified a novel function of TB4 in endothelial-mesenchymal transformation (EMT) in cardiac valve endocardial cushions in zebrafish. The expressions of thymosin family members in developing zebrafish embryos were determined by whole mount in situ hybridization. Of the thymosin family members only zTB4 was expressed in the developing heart region. Cardiac valve development at 48 h post fertilization was defected in zebrafish TB4 (zTB4) morpholino-injected embryos (morphants). In zTB4 morphants, abnormal linear heart tube development was observed. The expressions of bone morphogenetic protein (BMP) 4, notch1b, and hyaluronic acid synthase (HAS) 2 genes were also markedly reduced in atrio-ventricular canal (AVC). Endocardial cells in the AVC region were stained with anti-Zn5 antibody reactive against Dm-grasp (an EMT marker) to observe EMT in developing cardiac valves in zTB4 morphants. EMT marker expression in valve endothelial cells was confirmed after transfection with TB4 siRNA in the presence of transforming growth factor ${\beta}$ ($TGF{\beta}$) by RT-PCR and immunofluorescent assay. Zn5-positive endocardial AVC cells were not observed in zTB4 morphants, and knockdown of TB4 suppressed TGF-${\beta}$-induced EMT in ovine valve endothelial cells. Taken together, our results demonstrate that TB4 plays a pivotal role in cardiac valve formation by increasing EMT.

• 한국강구조학회 논문집
• /
• 제10권1호통권34호
• /
• pp.85-99
• /
• 1998

본 논문은 케이슨의 인양작업중 Fitting Anchor의 취성파괴로 인한 들고리의 붕괴원인을 조사하기 위한 실험적 연구이다. 또한, 본 연구에서는 응력해석을 통하여 들고리의 붕괴메카니즘을 분석하고 이를 실제 붕괴과정과 비교한다. 본 연구에서는 파괴된 강재에 대한 파면해석 뿐만아니라 화학성분시험, 인장시험 및 샤르피 V-노치 충격시험을 실시한다. 그리고 이의 시험 결과를 정상적인 강재에 대한 시험결과와 비교한다. 본 연구의 거시적, 미시적인 방법으로 결함을 관찰한 결과, Fitting Anchor 내부에 원주방향으로 나타난 표면결함은 케이슨의 진수시 발생하는 들고리의 인장응력이 작용하기 전에 발생한 것임을 확인할 수 있었다. 이는 균열선단의 응력집중이 발생하여 작용응력보다 큰 응력이 결함에 발생한 것임을 알 수 있다. 또한, 잠재한 결함의 크기가 임계값 이상으로 증가하여 본 연구대상 강재의 응력확대계수가 증가하였을 것으로 판단된다. 그래서 균열선단의 응력확대계수가 본 강재의 파괴인성보다 크게 되어 케이슨의 인양작업중에 취성파괴를 일으킨 것으로 사료된다. 본 연구결과에 따르면 케이슨 들고리의 붕괴는 Fitting Anchor의 취성파괴로 발생한 것으로 사료된다.

• Nuclear Engineering and Technology
• /
• 제38권7호
• /
• pp.639-656
• /
• 2006

In order to apply the leak before break (LBB) concept to nuclear piping systems, the dynamic strain aging effect of low carbon steel materials has to be taken into account, in compliance with the requirements of the Korean Standard Review Guide (KSRG) 3.6.3-1. For this goal, J-R tests are needed for a range of various temperatures and loading rates, including dynamic loading conditions. In the dynamic loading J-R test, the unloading compliance method can not be applied to measure the crack growth and direct current potential drop (DCPD) method; this method also has a problem defining the crack initiation point. The normalization method is known as a very useful method to determine the J-R curve under dynamic loading because it does not need additional equipment or complicated loading sequences such as electric current or unloading. This method was accepted by the American Society for Testing and Materials (ASTM) as a standard test method E1820 A15 in 2001. However, it has not yet been clearly verified yet if the normalization method is sufficiently reliable to be applied to LBB. In this study, the basic background of the J-integral, LBB and dynamic loading J-R test are explained, and the current status for dynamic loading J-R test methods are reviewed from the view point of LBB for nuclear piping. In particular, the theoretical and historical background of the normalization method which has received attention recently, is summarized. Recent studies for this method are introduced and future works are suggested that may improve the reliability of LBB for nuclear piping.

• Nuclear Engineering and Technology
• /
• 제52권12호
• /
• pp.2949-2957
• /
• 2020

Fatigue crack growth model has been developed for dissimilar metal weld joints of a piping component under cyclic loading, where in the crack is located at the center of the weld in the circumferential direction. The fracture parameter, Stress Intensity Factor (SIF) has been computed by using principle of superposition as K_H + K_M. K_H is evaluated by assuming that, the complete specimen is made of the material containing the notch location. In second stage, the stress field ahead of the crack tip, accounting for the strength mismatch, the applied load and geometry has been characterized to evaluate SIF (K_M). For each incremental crack depth, stress field ahead of the crack tip has been quantified by using J-integral (elastic), mismatch ratio, plastic interaction factor and stress parallel to the crack surface. The associated constants for evaluation of K_M have been computed by using the quantified stress field with respect to the distance from the crack tip. Net SIF (K_H + K_M) computed, has been used for the crack growth analysis and remaining life prediction by Paris crack growth model. To validate the model, SIF and remaining life has been predicted for a pipe made up of (i) SA312 Type 304LN austenitic stainless steel and SA508 Gr. 3 Cl. 1. Low alloy carbon steel (ii) welded SA312 Type 304LN austenitic stainless-steel pipe. From the studies, it is observed that the model could predict the remaining life of DMWJ piping components with a maximum difference of 15% compared to experimental observations.

• Genomics & Informatics
• /
• 제20권2호
• /
• pp.20.1-20.13
• /
• 2022

Recent studies have focused on the early detection of ovarian cancer (OC) using tumor materials by liquid biopsy. The mechanisms of microRNAs (miRNAs) to impact OC and signaling pathways are still unknown. This study aims to reliably perform functional analysis of previously validated circulating miRNAs' target genes by using pathfindR. Also, overall survival and pathological stage analyses were evaluated with miRNAs' target genes which are common in the The Cancer Genome Atlas and GTEx datasets. Our previous studies have validated three downregulated miRNAs (hsa-miR-885-5p, hsa-miR-1909-5p, and hsa-let7d-3p) having a diagnostic value in OC patients' sera, with high-throughput techniques. The predicted target genes of these miRNAs were retrieved from the miRDB database (v6.0). Active-subnetwork-oriented Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was conducted by pathfindR using the target genes. Enrichment of KEGG pathways assessed by the analysis of pathfindR indicated that 24 pathways were related to the target genes. Ubiquitin-mediated proteolysis, spliceosome and Notch signaling pathway were the top three pathways with the lowest p-values (p < 0.001). Ninety-three common genes were found to be differentially expressed (p < 0.05) in the datasets. No significant genes were found to be significant in the analysis of overall survival analyses, but 24 genes were found to be significant with pathological stages analysis (p < 0.05). The findings of our study provide in-silico evidence that validated circulating miRNAs' target genes and enriched pathways are related to OC and have potential roles in theranostics applications. Further experimental investigations are required to validate our results which will ultimately provide a new perspective for translational applications in OC management.