• Molecules and Cells
• /
• v.40 no.12
• /
• pp.945-953
• /
• 2017

We report the biological functions of a zebrafish homologue of RING-finger protein 152 (rnf152) during embryogenesis. rnf152 was initially identified as a brain-enriched E3 ligase involved in early embryogenesis of zebrafish. Expression of rnf152 was ubiquitous in the brain at 24 hpf but restricted to the eyes, midbrain-hindbrain boundary (MHB), and rhombomeres at 48 hpf. Knockdown of rnf152 in zebrafish embryos caused defects in the eyes, MHB, and rhombomeres (r1-7) at 24 hpf. These defects in rnf152-deficient embryos were analyzed by whole-mount in situ hybridization (WISH) using neuroD, deltaD, notch1a, and notch3 probes. NeuroD expression was abolished in the marginal zone, outer nuclear layer (ONL), inner nuclear layer (INL), and ganglion cell layer (GCL) of the eyes at 27 hpf. Furthermore, deltaD and notch1a expression was remarkably reduced in the ONL, INL, subpallium, tectum, cerebellum, and rhombomeres (r1-7) at 24 hpf, whereas notch3 expression was reduced in the tectum, cerebellum, and rhombomeres at 24 hpf. Finally, we confirmed that expression of Notch target genes, her4 and ascl1a, also decreased significantly in these areas at 24 hpf. Thus, we propose that Rnf152 is essential for development of the eyes, midbrain and hindbrain, and that Delta-Notch signaling is involved.

• Proceedings of the KWS Conference
• /
• v.43
• /
• pp.275-277
• /
• 2004

Fictitious notch radius approach is based on the Neuter's microstructural support hypothesis which assumes that fatigue crack is governed by highly stressed volume of the material right on the weld toe area rather than the surface stress at a pin point of weld toe area. Variety of successes have been achieved in applying this methodology to the fatigue of welded joint, hence, it became one of recommended design procedure in IIW's recommendation as well as many ship classification societies. 1mm fictitious notch radius approach was applied to the various fatigue problems of welded joints in this study covering the effect of weld size, notch stress calculation for 3D geometry and low cycle fatigue problem. It was found that fictitious notch radius approach fumed out to be very effective and accurate in dealing with fatigue strength of welded joint.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.59 no.1
• /
• pp.176-183
• /
• 2010

This paper presents some practical design issues that should be carefully considered when a notch filter is included in a linear feedback controller. A notch filter is generally used to compensate the effects of resonant modes that may result in poor performance. It is very common that the practical engineers prefer to add such a notch filter after having previously designed a feedback controller without the filter. It is known that the resulting performance by this approach is not seriously different from when a feedback controller is designed for a plant previously compensated by a notch filter. However, we will point out that there are some cases where both approaches have different performances. In order to show this, a low-order controller design using the partial model matching method has been applied to a linear time invariant (L Tn model. The results suggest that there is a tendency to achieve much better time responses in terms of reducing the overshoot and shortening the settling time, and in the frequency domain characteristics such as the sensitivity function and the stability margins when the design of a feedback controller after including a notch filter is carried out.

• The Korean Journal of Physiology and Pharmacology
• /
• v.19 no.6
• /
• pp.515-522
• /
• 2015

Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.8 no.2
• /
• pp.127-132
• /
• 1984

In this paper, stress concentration factor and distribution of slotted or notched plate which is subjected to uniaxial tensile load are studied. The experimental measurements have shown the following; (1)The stress around slot or notch of slotted or notched plate which is subjected to uniaxial tensile load is state of biaxial stress, which is mainly varied to notch radius and depth. (2)The stress concentration factor around slot or notch is mainly influenced by the .sigma.$_{yy}$ , it is varied with notch radius and depth. (3)For the notched specimen, there is a notch depth where stress concentration factor is maximum. On the other hand, for the slotted specimen, stress concentration factor increases as the notch depth increases. An investigation of the relationship between fracture and stress concentration factor due to the slot or notch will be presented on the later paper, for reference.

• Transactions of Materials Processing
• /
• v.18 no.1
• /
• pp.59-66
• /
• 2009

In this study, V-notch part has been considered as one of safety components in rectangular cup used for mobile device. This kind of safety component in rectangular cup with the V-notch part, which controls adequately the increased internal pressure in the rectangular cup, plays an important role to prevent the explosion from the excessive internal pressure. The protecting mechanism on the mobile device against the explosion is that a series of fracture on the V-notch part at the critical internal pressure level occurs. Therefore, it is very crucial to estimate accurately the working pressure range of the safety device. Relationship between the working internal pressure and fracture phenomenon at V-Notch part was investigated through numerical analysis using ductile fracture criteria. Integral value, I, of the used ductile fracture criteria was calculated from effective stress and strain, and then the bursting pressure of the V-notch part was extracted. Comparisons between the estimated and experimental results show that this systematic approach to predict bursting pressure using the ductile fracture criteria gives fairly good agreements.

• BMB Reports
• /
• v.50 no.1
• /
• pp.1-2
• /
• 2017

Acquiring a selective growth advantage by breaking the proliferation barrier established by gatekeeper genes is a centrally important event in tumor formation. Removal of the mammalian Hippo kinase Mst1 and Mst2 in hepatocytes leads to rapid hepatocellular carcinoma (HCC) formation, indicating that the Hippo signaling pathway is a critical gatekeeper that restrains abnormal growth in hepatocytes. By rigorous genetic approaches, we identified an interacting network of the Hippo, Wnt/${\beta}$-catenin and Notch signaling pathways that control organ size and HCC development. We found that in hepatocytes, the loss of Mst1/2 leads to the activation of Notch signaling, which forms a positive feedback loop with Yap/Taz (transcription factors controlled by Mst1/2). This positive feedback loop results in severe liver enlargement and rapid HCC formation. Blocking the Yap/Taz-Notch positive feedback loop by Notch inhibition in vivo significantly reduced the Yap/Taz activities, hepatocyte proliferation and tumor formation. Furthermore, we uncovered a surprising inhibitory role of Wnt/${\beta}$-catenin signaling to Yap/Taz activities, which are important in tumor initiation. Genetic removal of ${\beta}$-catenin in the liver of the Mst1/2 mutants significantly accelerates tumoriogenesis. Therefore, Wnt/${\beta}$-catenin signaling, known for its oncogenic property, exerts an unexpected function in restricting Yap/Taz and Notch activities in HCC initiation. The molecular interplay between the three signaling pathways identified in our study provides new insights in developing novel therapeutic strategies to treat liver tumors.

• Transactions of the Korean Society of Machine Tool Engineers
• /
• v.16 no.1
• /
• pp.1-8
• /
• 2007

The present study examined the mechanical properties of the friction welding zone of solid and hollow shafts made with SM20C according to the depth of the notch. Friction welding was conducted at welding conditions of 2,000 rpm, friction pressure of 60MPa, friction time of 1.4 seconds, upset pressure of 100MPa, and upset time of 2.0 seconds. In the tensile strength test, the tensile strength decreased as the depth of the notch increased. Tensile strength was moderately high when the depth of the notch was 2mm. The tensile strength of the welding zone increased as the friction revolution radius increased, because the latter led to the generation of adequate friction heat. According to the hardness test, hardness likewise increased as e friction revolution radius increased. In the bending test, the bend strength of the solid shaft decreased when the depth of the notch was 0-2mm but increased when the latter was 3-5mm. With regard to the hollow shaft, the bend strength drastically decreased when the depth of the notch was 3-4mm. Upon examination it was found that the microstructure became finer when the friction revolution radius increased.

• Steel and Composite Structures
• /
• v.28 no.1
• /
• pp.1-11
• /
• 2018

In this paper, local ratcheting behavior of 1045 steel plates with circular cutout was investigated. Experimental tests were carried out by a Zwick/Roell HB 100 servo hydraulic machine. In order to measure the local strain at notch root, a data acquisition system with strain gauge was used. Various notch diameters and distances of strain gauges mounted from the notch root were found influential in the magnitude of local ratcheting strain. It was found that the local maximum principal stress plays a crucial role in increasing the local plastic deformation. Numerical simulation was done by ABAQUS software using nonlinear isotropic/kinematic hardening model. Material parameters of hardening model were attained from several stabilized cycles of flat specimens subjected to symmetric strain cycles. The nonlinear kinematic hardening model along with the Neuber's rule was employed to assess local ratcheting at the notch root of steel plates. The results of the numerical simulations agreed closely with those measured values in this study. Both ratcheting progress and mean stress relaxation occurred simultaneously at the notch root.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.65 no.2
• /
• pp.279-285
• /
• 2016

In this paper, a cogging torque of IPM(Interior Permanent Magnet)-type BLDC motor is analyzed by FE program and the optimized notch on the rotor surface is designed to minimize the torque ripple. A differential evolution strategy algorithm and a response surface method are employed to optimize the rotor notch. In order to verify the proposed algorithm, an IPM BLDC motor is used, which is 50 kW, 8 poles, 48 slots and 1200 rpm at the rated speed. Its characteristics of the motor is calculated by FE program and 4 design variables are set on the rotor notch. The initial shape of the notch is like a non-symmetric half-elliptic and it is optimized by the developed algorithm. The cogging torque of the final model is reduced to $1.5[N{\cdot}m]$ from $5.2[N{\cdot}m]$ of the initial, which is about 71 % reduction. Consequently, the proposed algorithm for the cogging torque reduction of IPM-type BLDC motor using the rotor notch design seems to be very useful to a mechanical design for reducing noise and vibration.