• Clinical and Experimental Pediatrics
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• v.48 no.12
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• pp.1317-1323
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• 2005

Purpose : This study was performed to evaluate the recent frequency of karyotypes in different sex chromosome abnormalities and to evaluate the age and clinical manifestations at diagnosis. Methods : Peripheral blood leukocytes were obtained from subjects who were clinically suspected to have sex chromosome abnormalities and referred to the cytogenetic laboratory in the Department of Pediatrics, Kyungpook National University Hospital from February 1981 to August 2001. Results : The relative frequencies of different sex chromosome abnormalities were Klinefelter(52 percent), Turner(42 percent), XXX syndrome(3 percent) and mixed gonadal dysgenesis(3 percent). The populations of different karyotypes in Klinefelter syndrome were 47,XXY(97 percent) and 46,XY/47,XYY(3 percent). The populations of different karyotypes in Turner syndrome were 45,X(67 percent,), mosaicism(23 percent), and structural aberrations(10 percent). The populations of different karyotypes in XXX syndrome were 47,XXX(67 percent,) and 46,XX/47,XXX(33 percent). All mixed gonadal dysgenesis were 45,X/46,XY. Eighty one percent of sex chromosome abnormalities was diagnosed after puberty. Patients diagnosed with Klinefelter and Turner syndrome in infancy showed nearly normal phenotypes or had minor congenital malformations. Conclusion : Early diagnoses of sex chromosome abnormalities is required to prevent associated morbidities and to maximize growth and development. We have to pay careful attention in diagnoses of Turner syndrome because of the high proportion of mosaicism and structural aberrations.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.494-504
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• 2010

The effect of the addition of the fresh medium, volume of under solid medium in double layer culture as well as the medium change after pretreating microspores on the production of embryos in microspore culture of hot pepper (Capsicum annuum L.) has been studied. When cultured after heat pre-treatment, changing pretreatment media with fresh culture media proved to be more effective for embryo production rather than supplementing additional culture media. Heat-pretreating for 3 days turned out more effective for embryo production than pretreating for 1 or 2 days. In the case of anther pretreatment, the addition of fresh medium after culture was not effective for embryo production. In pretreating microspores, however, supplementing additional fresh culture media greatly improved embryo yield and quality. The best time point of media addition was 4 days after culture commenced, and the most effective number of times of media addition was one time addition. Moreover, the effective volume of added medium in double layer culture for embryo production was 1.5 ml. The addition of media more than 1.5 ml reduced both embryo yield and quality. Double layer medium was more effective for embryo development than liquid medium. When the volume of under solid medium increased ranging from 3 ml to 7 ml, more cotyledonary embryos were produced in either 5 ml or 7 ml compared to 3 ml, even though the total number of embryos were highest in 3 ml. These results can be used as an important data for establishing an efficient microspore culture system for producing high frequency of normal embryos in hot pepper.

• Pediatric Infection and Vaccine
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• v.4 no.2
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• pp.218-224
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• 1997

Purpose: The antimicrobial resistance of S. pneumoniae has encountered with increasing frequency from around the world. In our country, penicillin resistant strains of S. penumococci are rapidly increasing. It has been known that colonized pneumococci in upper respiratory tract cause sinisitis, otitis media, meningitis and pneumonia. We tried to reveal the colonization rate of pneumonocci in upper respiratory tract, their antimicrobial resistance and DNA fingerprinting pattern in normal children. Methods: We got specimens from 117 children of day-care center in Seoul through oropharyngeal swab. After incubation on BAP, optochin test and slide latex agglutination test were used for identification. Antimicobial susceptibility test to penicillin, vancomycin, erythromycin and TMP-SMZ was done with disk diffusion method. Penicillin MIC was gotten through the broth microdilution method. Genotyping of 45 pneumococci was done by rep-PCR using REP1R-Dt and REP2-Dt primer. Results: The carriage rate of pneumococci in the day-care center children was 38%(45/117). The resistance of penicillin, erhthromycin, TMP/SMZ, vancomycin by the disk diffusion method are 89%, 91%, 64% and 0%, respectively. 64% of the isolates showed multiple resistance. 7 types of DNA fingerprinting were gotten and 78% of isolates belonged to three types. Conclusion: We found that the antimicrobial resistance of children attending the day-care center in Seoul was much higher than expected. We assumed that this might be due to their easy and frequent exposure to antimicrobial agents and crowded day-care center environment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.3
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• pp.223-228
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• 2011

The present study was conducted to determine the optimum in vitro culture condition for callus induction and plant regeneration from mature seeds of bermudagrass (Cynodon dactylon cv. Common). It was revealed that mature seeds cultured on MS medium supplemented with 2 mg/L 2,4-D, 0.5 g/L proline, 0.5 g/L casamino acid and 3 g/L Gelrite under light condition produced the highest percentage of callus formation (39.2%). The most suitable medium for plant regeneration from dehydrated calli was MS agar medium supplemented with 0.5 mg/L 2,4-D, 2 mg/L BA, 0.5 g/L proline, 0.5 g/L casamino acid 3 g/L Gelrite which induced the highest percentage of calli forming shoots (57.7%). The frequency of callus induction and plant regeneration were the highest on sucrose, followed by maltose. The shoots were rooted at the highest rate (100%) when transferred onto 1/2 MS medium. Regenerated plants were morphologically uniform with normal growth pattern.

• Korean Journal of Food Science and Technology
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• v.46 no.5
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• pp.593-600
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• 2014

Normal rice starch (NRS) possesses high gelling and retrogradation tendencies, with poor freeze-thaw stability. This study investigated the effects of partial replacement of waxy rice starch (WRS) with gums on the pasting and viscoelastic properties as well as the freeze-thaw stability of the WRS paste. Xanthan gum (XAT), locust bean gum (LBG), and their mixtures were individually mixed with WRS at a ratio of 1:19 (w/w). WRS-gum mixtures were pasted using a rapid visco-analyzer at 5% total solid content, and analyzed with respect to the pasting and viscoelastic characteristics, and freeze-thaw stability. Pasting properties of WRS were retarded in pasting temperature and enhanced in pasting viscosity (although peak viscosity was varied) by partial replacement with gum and gum mixtures. Storage moduli of WRS-XAT:LBG pastes became similar to those of NRS paste with increasing angular frequency from 1 to 10 rad/s. Finally, WRS-XAT and WRS-XAT:LBG possessed more enhanced freeze-thaw stability than NRS.

• Nutritional Sciences
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• v.5 no.1
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• pp.34-39
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• 2002

The objective of tole study was to compare nutritional knowledge, attitudes and food practices of competitive athletes, recreational athletes and non-participants. All students enrolled at the University of Nebraska in an Introductory Nutrition course in which sports nutrition was stressed, were asked to participate in the study. The course was typical of many introductory nutrition courses ; about two thirds of the students were from the Department of Health, Physical Education and Recreation, and most of the students were encouraged to take this course by their advisers. The students were divided into three groups : 20 competitive athletes (mostly runners, swimmers, basketball players and football players for the University), 14 recreational athletes (who were involved in a planned exercise program at least 2 hours per week), and 19 non-participants. At the beginning of the semester, students completed a food frequency questionnaire, and a 24-hour food record based on the Food Guide Pyramid. A nutrition knowledge and attitude questionnaire was formulated specifically for this study, covering normal nutrition and sports nutrition. The results showed that consumption of foods from all Food Guide Pyramid food groups except the fats/oils/sweets group was not significantly different among the three student groups. Regarding the fats/oils/sweets group, competitive athletes consumed significantly more numbers of servings than recreational athletes, while the recreational athletes consumed significantly more than non-participants (p<0.01). The total mean scores from the nutrition attitude and knowledge questionnaire were not significantly different among the three student groups. However, combined athletes (competitive athletes and recreational athletes) obtained significantly higher scores on some of the nutrition attitude questions (p<0.05). The results of the study indicate that athletes, significantly lower scores on some of the nutrition knowledge questions (p<0.05). The results of the study indicate that athletes, whether competitive or some of the nutrition knowledge questions (p<0.05). The results of the study indicate that athletes, whether competitive or recreational, had a very positive attitude regarding the effects of nutrition on their sports performance and health: however, these athletes did not appear to apply scientific principles of sports nutrition to their diet. In conclusion, advising athletes to simply increase or decrease their intakes of certain foods is too simplistic an approach for maintenance of good health and for better sporting performance. Athletes and non-athletes need continuous nutrition education to help with improving their competitive edge and food choices.

• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.102-108
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• 1986

This study was carried out to examine the effects of seasonality, age, abnormalities of reproductive tract and the frequency of superovulatory treatments on the superovulatory responses and rate of pregnancy in repeat breeder for embryo transfer. Thirty eight eggs were obtained from 6 donors by flushing the uterine horns and 33 embryos were transfered to 33 recipients. (29 holstein heifers, 3 holstein cows and 1 Korean native heifer). From this study, the following results could be obtained: 1. It seemed that spring (Feb.-Apr.) was the better season than any others to produce good embryos. Rate of pregnancy decreased significantly after August because of low heating rate. 2. Rate of pregnancy in the cows with normal reproductive tract showed much better superovulatory responses (80%) than the cows with abnormal reproductive tract (33%), as expected. 3. 8 years old cows produced higher rater of pregnancy than 4 years old cow (79.3 vs 50), and also in superovulatory responses, 8 years old cows showed slightly better results than 4 years old cow. 4. The number of ovulated eggs and rate of pregnancy in the repeated superovulatory treatment more than once were 41 eggs and 85.7%, and those of the donors treated the first superovulatory treatment were 32 eggs and 68.4%, respectively. 5. These results are showing the possibility that utilizing the repeat breeders as donors in embryo transfer could be able to elevate their economic values.

• Journal of Korean Society of Forest Science
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• v.25 no.1
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• pp.80-84
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• 1975

The seeds, scions and plants of chestnut tree (Castanea. crenata) and Chinese chestnut tree (C. bungeana) were irradiated by gamma ray in order to know their biological effects on germination, plant growth and mutation at several accumulative doses and dose rates. The results of this study could be summarized as follows: 1. In general, the radio-sensitivity of scions was more sensitive to irradiation, showing 50% reduction dose of the control for grafting percentage at 3.1 kR as compared with 4.6 kR for the above reduction dose in germination rates of seeds. 2. The seeds treated by 5 kR dose resulted in some albino mutants at a rate of 0.84%. There was a general tendency that the seedling height reduced significantly as the irradiation dose increased. 3. The scions treated by an acute irradiation showed their 50% reduction dose in grafting at 3.2 kR for Chinese chestnut and at 3.1 kR for chestnut, respectively, while their irradiation doses increased three times, having 10.2 kR for the 50% reduction dose in the case of semi-acute irradiation. 4. When Chinese chestnut trees were irradiated during their dormant period with a total dose of 7.5 kR to 4.9 kR at a dose rate of 150 R to 98 R per day, there were induced giant leaf bud-sports at a frequency of 16.6%. The averag leaf area of the giant leaf bud-sports were measured at 96.36 square centimeters, while the area of normal leaf was only 26.28 square centimeters.

• Women's Health Nursing
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• v.4 no.1
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• pp.84-102
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• 1998

In this study the levels and influencing characteristics of maternal identity and self confidence for caring the baby were identified during the immediate postpartum period. 114 primiparous women who delivered vaginally normal baby participated in the survey from August 1 to October 31, 1996. SD scale was to measure maternal identity which consisted of 11 items for mother and 6 items for baby. Likert scale was to measure self confidence for caring the baby (38 items). Cronbach's alphas for evaluating internal consistency as follows : .86 for maternal identity and .96 for self confidence scale for caring the baby. The study showed these results : 1. Mean score of maternal identity(82.03 : 52.65 for mother, 29.38 for baby) was considered relatively low. 2. Mean score of self confidence for caring the baby(113.91) was considered relatively low. 3. There were significant influencing characteristics of primiparous to maternal identity : age(total ; F=3.53, p=.0329, for mother ; F=2.60, p=.0719, for baby ; F=3.12, p=.0481), prenatal infant care preparation(total ; t=2.31, p=.0306, for mother ; F=2.62, p=.0160), knowledge about infant care during the hospitalization(for baby ; F=3.94, p=.02222), colostrum feeding(total ; t=1.95, p=.0541, for baby ; t=2.71, p=.0080), frequency of breast feeding during the hospitalization(for baby ; F=3.91, p=.0228)and feeding type after discharge(for baby ; F=3.18, p=.0456). 4. There were significant influencing characteristics of primiparous to self confidence for caring the baby : routine husband support(F=6.09, p=.0031), prenatal infant care preparation(t=2.04, p=.0574), knowledge about infant care during the hospitalization(F=3.15, p=.0467), education of breast feeding during the hospitalization(t=-1.79, p=.0850). 5. Correlation between maternal identity and self confidence for caring the baby was r=.37608(p=.0001). This study implies that special education programs for primipara are needed. For the future, this study suggest that maternal role variables need to be monitored through the home visiting follow up. Also intervention programs related to prenatal care, discharge education, home visiting follow up need to be developed and then evaluated their effectiveness.

• FLOWER RESEARCH JOURNAL
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• v.18 no.2
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• pp.136-141
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• 2010

In order to analyze the horticultural-therapy program, which was carried out targeting the mentally disabled, relevant 559 copies of 'Confirmation Note of horticultural activity' submitted for to be used the use in license examination to Korean Horticultural Therapy and Wellbeing Association were used as a tool. It contains 64 horticultural therapists for level 1 and 524 horticultural therapists for level 2 from May in 2000 to February in 2008. With the aim of examining difference depending on people covered by the program, license kind and horticultural therapy activity period in horticulture therapists, ${\chi}^2$ test was conducted on the basis of frequency in each. Data was analyzed by using SPSS (Statistical Package for the Social Science) Win 13.0 program, which was carried out targeting the mentally disabled, the 'art and craft activity' was the largest with 46.0%. In terms of 'growing activity', the 'normal growing' showed the highest ratio with 74.7%. In the 'art and craft activity', the 'flower decoration' showed the highest ratio with 37.5%. In the result of 'Cooking activity', the activity related to 'tea' was the largest ratio with 33.6%. As a result of 'learning activity', 'orientation' was the largest ratio with 47.6%. In the 'outdoor activity', 'excursion' was the largest ratio with 36.7%.